Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,266,318 G→T 100% R307L (CGT→CTT)  yjcC → putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,266,3180GT98.9% 932.5 / 0.4 284R307L (CGT→CTT) yjcCputative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (147/134);  total (148/136)
Fisher's exact test for biased strand distribution p-value = 6.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 289 total aligned reads displayed.

TTACTCTGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACGTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGA  >  CP009273/4266173‑4266462
                                                                                                                                                 |                                                                                                                                                
ttACTCTGGCTACGTATTCGACAAAACTATTTATCACCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACttt                                                                                                                                                >  2:1137492/1‑148 (MQ=255)
   ctctGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGc                                                                                                                                             <  1:1800071/148‑1 (MQ=255)
    tctGGCTACGTATTCGACAAAACTATTTATCACCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGcc                                                                                                                                            >  2:2202134/1‑148 (MQ=255)
     ctGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCt                                                                                                                                           >  2:2285402/1‑148 (MQ=255)
     ctGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCt                                                                                                                                           <  1:1986704/148‑1 (MQ=255)
     ctGGCTACGTATTCGACAAAACTATTTATCACCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCt                                                                                                                                           <  2:1365916/148‑1 (MQ=255)
        gCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGt                                                                                                                                        <  2:79126/148‑1 (MQ=255)
             gTATTCGAAAAAACTATTTATCACCAAAACGTAAATTGCAACGCGCCCCCGAAAAACATCAACTTTGTCTTTTTTACCATCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCa                                                                                                                                   <  1:2700304/148‑1 (MQ=255)
              tATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAg                                                                                                                                  <  1:1022617/148‑1 (MQ=255)
              tATTCGACAAAACTATTTATCACCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAg                                                                                                                                  >  1:2643357/1‑148 (MQ=255)
               aTTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAgg                                                                                                                                 <  1:559990/148‑1 (MQ=255)
                 tCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCGTGGTGAGCAgggg                                                                                                                               >  2:758606/1‑148 (MQ=255)
                 tCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAgggg                                                                                                                               <  2:49280/148‑1 (MQ=255)
                    aCAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCaa                                                                                                                            <  2:2750211/148‑1 (MQ=255)
                    aCAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCaa                                                                                                                            >  2:1961306/1‑148 (MQ=255)
                    aCAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCaa                                                                                                                            <  2:3758820/148‑1 (MQ=255)
                        aaCTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTGGTTACTTTGGCCTGGTGAGCAGGGGCAaataa                                                                                                                        >  2:395693/1‑148 (MQ=255)
                        aaCTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACAAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAaataa                                                                                                                        <  1:434305/148‑1 (MQ=255)
                         aCTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAaataat                                                                                                                       >  1:1271786/1‑148 (MQ=255)
                           tATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGa                                                                                                                     <  1:1745916/148‑1 (MQ=255)
                            aTTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGaa                                                                                                                    >  2:755534/1‑148 (MQ=255)
                            aTTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGaa                                                                                                                    >  1:3326486/1‑148 (MQ=255)
                            aTTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGaa                                                                                                                    <  2:2266565/148‑1 (MQ=255)
                              ttATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATc                                                                                                                  <  2:3088744/148‑1 (MQ=255)
                                 tctcCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCgg                                                                                                               >  1:1592419/1‑148 (MQ=255)
                                      cAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCagag                                                                                                          <  2:564077/148‑1 (MQ=255)
                                        aaCGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGtt                                                                                                        >  2:2792693/1‑148 (MQ=255)
                                        aaCGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGtt                                                                                                        <  2:2232863/148‑1 (MQ=255)
                                         aCGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGttt                                                                                                       <  2:206863/148‑1 (MQ=255)
                                           gTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTAt                                                                                                     <  1:2070295/148‑1 (MQ=255)
                                            tAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTAtt                                                                                                    <  2:3078399/148‑1 (MQ=255)
                                             aaaTTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTc                                                                                                   <  2:3158621/148‑1 (MQ=255)
                                             aaaTTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTc                                                                                                   >  2:3645917/1‑148 (MQ=255)
                                             aaaTTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTc                                                                                                   >  1:753923/1‑148 (MQ=255)
                                               aTTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCg                                                                                                 >  2:1193634/1‑148 (MQ=255)
                                               aTTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCg                                                                                                 >  2:2379644/1‑148 (MQ=255)
                                                 tGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCt                                                                                               >  2:1665761/1‑148 (MQ=255)
                                                     aCGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCa                                                                                           >  1:2190517/1‑148 (MQ=255)
                                                          cccTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGaaaa                                                                                      <  1:614204/148‑1 (MQ=255)
                                                              cGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAaggag                                                                                  >  1:3127757/1‑148 (MQ=255)
                                                                  aaaCATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGga                                                                              <  1:3713692/148‑1 (MQ=255)
                                                                    aCATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGgatg                                                                            >  2:1799961/1‑148 (MQ=255)
                                                                    aCATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGgatg                                                                            >  2:2698747/1‑148 (MQ=255)
                                                                    aCATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGgatg                                                                            <  1:1450806/148‑1 (MQ=255)
                                                                     cATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGgatga                                                                           <  1:2792693/148‑1 (MQ=255)
                                                                     cATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGgatga                                                                           >  2:2956315/1‑148 (MQ=255)
                                                                     cATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGgatga                                                                           <  2:184/148‑1 (MQ=255)
                                                                     cATCAACTGTGTCATTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGgatga                                                                           <  2:2430814/148‑1 (MQ=255)
                                                                       tCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATa                                                                         >  2:1004185/1‑148 (MQ=255)
                                                                         aaCTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGa                                                                       <  2:433800/148‑1 (MQ=255)
                                                                          aCTTTGTCTTTATTCCCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCGGGCAGAAAAGGAGGGGATGATAGaa                                                                      <  1:1477697/148‑1 (MQ=255)
                                                                            tttGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACa                                                                    >  2:3029052/1‑148 (MQ=255)
                                                                            tttGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTAATTTGGACTTGTGATCAGGGGCAAATAATTAATCCGGCAGAGTTTATTACGCTGGCAGAAAAGGAGGGGATGATAGAACa                                                                    >  1:2922529/1‑148 (MQ=255)
                                                                             ttGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAg                                                                   <  1:3280658/148‑1 (MQ=255)
                                                                               gTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCTGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGAt                                                                 <  1:1825975/148‑1 (MQ=255)
                                                                                tCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATa                                                                >  2:466426/1‑148 (MQ=255)
                                                                                tCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATa                                                                <  2:3711306/148‑1 (MQ=255)
                                                                                 cTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATaa                                                               <  2:1701635/148‑1 (MQ=255)
                                                                                 cTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATaa                                                               >  2:1245413/1‑148 (MQ=255)
                                                                                 cTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATaa                                                               >  2:3765550/1‑148 (MQ=255)
                                                                                  tttATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAAc                                                              <  2:619815/148‑1 (MQ=255)
                                                                                  tttATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAAc                                                              <  1:3604366/148‑1 (MQ=255)
                                                                                   ttattaCCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAAAAGATAACt                                                             >  1:655662/1‑148 (MQ=255)
                                                                                      ttaCCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGAt                                                          <  1:404123/148‑1 (MQ=255)
                                                                                      ttaCCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGAt                                                          <  1:3503705/148‑1 (MQ=255)
                                                                                      ttaCCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGAt                                                          >  1:1522046/1‑148 (MQ=255)
                                                                                       taCCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGAtt                                                         <  1:2853441/148‑1 (MQ=255)
                                                                                        aCCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTa                                                        <  2:1581368/148‑1 (MQ=255)
                                                                                        aCCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTa                                                        >  2:837797/1‑148 (MQ=255)
                                                                                        aCCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTa                                                        >  2:3172204/1‑148 (MQ=255)
                                                                                        aCCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTa                                                        >  2:1807173/1‑148 (MQ=255)
                                                                                         ccAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTAt                                                       <  2:1425755/148‑1 (MQ=255)
                                                                                         ccAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTAt                                                       <  2:1062271/148‑1 (MQ=255)
                                                                                              cAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTAt                                                  <  2:2887686/148‑1 (MQ=255)
                                                                                              cAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTAt                                                  >  1:3040312/1‑148 (MQ=255)
                                                                                                  aatCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGAt                                              >  1:3427435/1‑148 (MQ=255)
                                                                                                    tCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATaa                                            >  1:2446507/1‑148 (MQ=255)
                                                                                                       atatCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGt                                         <  1:441189/148‑1 (MQ=255)
                                                                                                          tCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGACTCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCtt                                      >  2:3531428/1‑148 (MQ=255)
                                                                                                          tCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCtt                                      >  2:3530179/1‑148 (MQ=255)
                                                                                                           cAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTc                                     >  1:1666536/1‑148 (MQ=255)
                                                                                                              aaCAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCcgc                                  >  1:1062766/1‑148 (MQ=255)
                                                                                                              aaCAGAAAAATGTATCGGCGCTGAAGCGGTGTTAATTTGGCCAGGGGAGCACGGGCAAATAATGAATCCGGCACAGATTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCcgc                                  >  1:2981108/1‑148 (MQ=255)
                                                                                                               aCAGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCcgcg                                 >  2:1869954/1‑148 (MQ=255)
                                                                                                                 aGAAAAATGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGAt                               <  1:1134648/148‑1 (MQ=255)
                                                                                                                   aaaaaTGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCt                             >  2:2053816/1‑148 (MQ=255)
                                                                                                                   aaaaaTGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCt                             >  1:3213690/1‑148 (MQ=255)
                                                                                                                    aaaaTGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTg                            <  2:2554247/148‑1 (MQ=255)
                                                                                                                       aTGTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGc                         >  2:2364079/1‑148 (MQ=255)
                                                                                                                         gTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGa                       <  2:3336547/148‑1 (MQ=255)
                                                                                                                         gTATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGa                       >  1:2828648/1‑148 (MQ=255)
                                                                                                                          tATCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGAt                      <  1:770998/148‑1 (MQ=255)
                                                                                                                           aTCGGCGCTGAAGCGTTGTTACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGAtt                     >  2:3616220/1‑148 (MQ=255)
                                                                                                                                          ttgttACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATg      >  2:779260/1‑148 (MQ=255)
                                                                                                                                           tgttACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTTATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGc     <  1:2528764/148‑1 (MQ=255)
                                                                                                                                           tgttACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGc     >  2:911448/1‑148 (MQ=255)
                                                                                                                                           tgttACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGc     <  2:3437289/148‑1 (MQ=255)
                                                                                                                                              tACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGa  <  1:1915592/148‑1 (MQ=255)
                                                                                                                                              tACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGa  <  1:3697925/148‑1 (MQ=255)
                                                                                                                                              tACTTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGa  <  1:582355/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TTACTCTGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACGTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGA  >  CP009273/4266173‑4266462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑