Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 329,372 A→T 100% L258Q (CTG→CAG)  yahB ← putative DNA‑bindng transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273329,3720AT99.3% 895.4 / ‑2.7 272L258Q (CTG→CAG) yahBputative DNA‑bindng transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base T (149/120);  total (150/122)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 277 total aligned reads displayed.

CAGTAAATCCTGATAGATCCCCGTTAATATGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCAGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTTTATTAA  >  CP009273/329227‑329519
                                                                                                                                                 |                                                                                                                                                   
cAGTAAATCCTGATAGATCCCCGTTAATATGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACctgc                                                                                                                                                   >  2:2394924/1‑148 (MQ=255)
   tAAATCCTGATAGATCCCCGTTAATATGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAgg                                                                                                                                                >  2:1700935/1‑148 (MQ=255)
   tAAATCCTGATAGATCCCCGTTAATATGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAgg                                                                                                                                                >  1:3654187/1‑148 (MQ=255)
    aaaTCCTGATAGATCCCCGTTAATATGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAggg                                                                                                                                               <  2:2686616/148‑1 (MQ=255)
    aaaTCCTGATAGATCCCCGTTAATATGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAggg                                                                                                                                               <  2:3108049/148‑1 (MQ=255)
      aTCCTGATAGATCCCCGTTAATATGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGAt                                                                                                                                             >  2:1166062/1‑148 (MQ=255)
       tCCTGATAGATCCCCGTTAATATGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGAtt                                                                                                                                            >  1:2397872/1‑148 (MQ=255)
             tAGATCCCCGTTAATATGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCa                                                                                                                                      <  2:3023353/148‑1 (MQ=255)
             tAGATCCCCGTTAATATGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCa                                                                                                                                      <  1:3044084/148‑1 (MQ=255)
              aGATCCCCGTTAATATGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCAt                                                                                                                                     >  2:100531/1‑148 (MQ=255)
               gATCCCCGTTAATATGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATc                                                                                                                                    >  1:1486497/1‑148 (MQ=255)
               gATCCCCGTTAATATGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATc                                                                                                                                    >  2:1341841/1‑148 (MQ=255)
                  ccccGTTAATATGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGcc                                                                                                                                 <  2:3491383/148‑1 (MQ=255)
                   cccGTTAATATGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCt                                                                                                                                <  1:310091/148‑1 (MQ=255)
                    ccGTTAATATGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTc                                                                                                                               >  1:389720/1‑148 (MQ=255)
                        tAATATGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACgg                                                                                                                           >  2:3328604/1‑148 (MQ=255)
                        tAATATGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACgg                                                                                                                           >  2:3329508/1‑148 (MQ=255)
                          atatGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGAc                                                                                                                         >  2:3383455/1‑148 (MQ=255)
                           tatGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGAcc                                                                                                                        >  1:3722946/1‑148 (MQ=255)
                            atGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCa                                                                                                                       >  1:851877/1‑148 (MQ=255)
                            atGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCa                                                                                                                       >  2:412560/1‑148 (MQ=255)
                             tGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCAt                                                                                                                      >  1:2109887/1‑148 (MQ=255)
                              gCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATg                                                                                                                     >  2:2337719/1‑148 (MQ=255)
                              gCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATg                                                                                                                     >  1:2647029/1‑148 (MQ=255)
                              gCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATg                                                                                                                     >  1:2746174/1‑148 (MQ=255)
                                cATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTa                                                                                                                   <  1:252525/148‑1 (MQ=255)
                                cATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTa                                                                                                                   <  2:750301/148‑1 (MQ=255)
                                 aTTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTaa                                                                                                                  >  1:62141/1‑148 (MQ=255)
                                 aTTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTaa                                                                                                                  >  1:62313/1‑148 (MQ=255)
                                  tttGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAAt                                                                                                                 >  2:1399307/1‑148 (MQ=255)
                                   ttGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATc                                                                                                                <  2:1183150/148‑1 (MQ=255)
                                     ggCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCg                                                                                                              <  1:2664056/148‑1 (MQ=255)
                                      gcgcAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCgg                                                                                                             >  2:609366/1‑148 (MQ=255)
                                           aaTTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCaaaa                                                                                                        >  2:2772862/1‑148 (MQ=255)
                                           aaTTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCaaaa                                                                                                        >  1:300700/1‑148 (MQ=255)
                                           aaTTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCaaaa                                                                                                        >  2:3293021/1‑148 (MQ=255)
                                            aTTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCaaaga                                                                                                       >  2:1985329/1‑146 (MQ=255)
                                            aTTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCaaaaa                                                                                                       >  1:3512770/1‑148 (MQ=255)
                                             ttGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCaaaaaa                                                                                                      >  2:213026/1‑148 (MQ=255)
                                             ttGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCaaaaaa                                                                                                      >  1:1401205/1‑148 (MQ=255)
                                              tGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAAc                                                                                                     >  1:3608496/1‑148 (MQ=255)
                                               gTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAAcc                                                                                                    >  1:2556162/1‑148 (MQ=255)
                                                ttttttAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAACCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCa                                                                                                   <  2:481861/148‑1 (MQ=255)
                                                 tttttAATCCACTGCGTCACCTGACCCGTAGCAGAATGTTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCaa                                                                                                  <  2:876754/148‑1 (MQ=255)
                                                 tttttAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCaa                                                                                                  <  2:2647432/148‑1 (MQ=255)
                                                 tttttAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCaa                                                                                                  <  2:2647029/148‑1 (MQ=255)
                                                     tAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTcc                                                                                              <  1:86330/148‑1 (MQ=255)
                                                      aaTCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCt                                                                                             <  1:3281288/148‑1 (MQ=255)
                                                       aTCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCtt                                                                                            <  2:2948655/148‑1 (MQ=255)
                                                       aTCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCtt                                                                                            <  2:121129/148‑1 (MQ=255)
                                                        tCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTc                                                                                           <  1:897774/148‑1 (MQ=255)
                                                         ccACTGCGTCACCTGACCCGTATCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCa                                                                                          >  1:1950339/1‑148 (MQ=255)
                                                           aCTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCAcc                                                                                        >  1:2532828/1‑148 (MQ=255)
                                                            cTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCAGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCt                                                                                       >  1:169063/1‑148 (MQ=255)
                                                                gTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTaaaa                                                                                   <  2:2581133/148‑1 (MQ=255)
                                                                 tCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAAt                                                                                  <  1:1700935/148‑1 (MQ=255)
                                                                 tCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAAt                                                                                  <  1:492640/148‑1 (MQ=255)
                                                                  cACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATc                                                                                 >  1:272860/1‑148 (MQ=255)
                                                                  cACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATc                                                                                 >  1:3755942/1‑148 (MQ=255)
                                                                       gACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCa                                                                            <  1:1511591/148‑1 (MQ=255)
                                                                       gACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCa                                                                            <  1:70989/148‑1 (MQ=255)
                                                                       gACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCa                                                                            >  2:2487182/1‑148 (MQ=255)
                                                                        aCCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCAc                                                                           >  2:960850/1‑148 (MQ=255)
                                                                        aCCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCAc                                                                           >  1:3622878/1‑148 (MQ=255)
                                                                         cccGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACt                                                                          <  2:3654187/148‑1 (MQ=255)
                                                                          ccGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTg                                                                         <  2:2085820/148‑1 (MQ=255)
                                                                                cAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACAttt                                                                   <  2:1687802/148‑1 (MQ=255)
                                                                                   aaTGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGgt                                                                >  1:1058107/1‑148 (MQ=255)
                                                                                    aTGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGgtg                                                               <  2:200334/148‑1 (MQ=255)
                                                                                     tGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGgtgt                                                              <  2:947646/148‑1 (MQ=255)
                                                                                       cTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCATCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTg                                                            <  1:1341841/148‑1 (MQ=255)
                                                                                          aGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGaaa                                                         <  1:1166062/148‑1 (MQ=255)
                                                                                          aGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGaaa                                                         <  1:2368081/148‑1 (MQ=255)
                                                                                            tCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATc                                                       <  2:2397872/148‑1 (MQ=255)
                                                                                            tCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATc                                                       <  1:1874780/148‑1 (MQ=255)
                                                                                                cAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGc                                                   >  1:1449573/1‑148 (MQ=255)
                                                                                                     aCATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAg                                              >  1:1029187/1‑148 (MQ=255)
                                                                                                      cATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGa                                             <  1:411373/148‑1 (MQ=255)
                                                                                                       aTGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGaa                                            <  1:2605413/148‑1 (MQ=255)
                                                                                                       aTGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGaa                                            <  1:590349/148‑1 (MQ=255)
                                                                                                        tGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAAt                                           <  2:3241527/148‑1 (MQ=255)
                                                                                                           gcgAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGa                                        <  2:1539670/148‑1 (MQ=255)
                                                                                                           gcgAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGa                                        <  1:3329502/148‑1 (MQ=255)
                                                                                                           gcgAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGa                                        <  1:3328604/148‑1 (MQ=255)
                                                                                                           gcgAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGa                                        <  2:3440392/148‑1 (MQ=255)
                                                                                                             gAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACt                                      >  2:3558971/1‑148 (MQ=255)
                                                                                                               aTCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTcc                                    >  2:589506/1‑148 (MQ=255)
                                                                                                                tCCTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCt                                   >  2:1857907/1‑148 (MQ=255)
                                                                                                                 ccTGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTg                                  >  1:232595/1‑148 (MQ=255)
                                                                                                                   tGGCGCGGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGcc                                >  2:683842/1‑148 (MQ=255)
                                                                                                                       gcgGATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTg                            <  2:2834766/148‑1 (MQ=255)
                                                                                                                         ggATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTgca                          <  1:2390380/148‑1 (MQ=255)
                                                                                                                         ggATTATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTgca                          <  1:468927/148‑1 (MQ=255)
                                                                                                                            ttATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTgcagca                       >  1:2043832/1‑148 (MQ=255)
                                                                                                                            ttATGGATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTgcagca                       <  1:2138416/148‑1 (MQ=255)
                                                                                                                                 gATTTGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAg                  >  1:2971559/1‑148 (MQ=255)
                                                                                                                                   tttGTCGGGTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGcc                <  1:3591907/148‑1 (MQ=255)
                                                                                                                                          ggTAACCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACtttt         <  1:2574046/148‑1 (MQ=255)
                                                                                                                                             aaCCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTtta      >  1:3048075/1‑148 (MQ=255)
                                                                                                                                              aCCTGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTttat     <  2:767683/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CAGTAAATCCTGATAGATCCCCGTTAATATGCCATTTGGCGCAAATTGTTTTTTAATCCACTGCGTCACCTGACCCGTAGCAGAATGCTGAGTCGCCAGTAACATGCGCGAATCCTGGCGCGGATTATGGATTTGTCGGGTAACCAGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTTTATTAA  >  CP009273/329227‑329519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑