Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,318,517 A→T 100% T522T (ACA→ACT)  crfC → clamp‑binding sister replication fork colocalization protein, dynamin‑related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,318,5170AT97.4% 661.3 / 4.8 206T522T (ACA→ACT) crfCclamp‑binding sister replication fork colocalization protein, dynamin‑related
Reads supporting (aligned to +/- strand):  ref base A (3/3);  new base T (87/113);  total (90/116)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 206 total aligned reads displayed.

CGAGATTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGTT  >  CP009273/4318373‑4318660
                                                                                                                                                |                                                                                                                                               
cgAGATTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTg                                                                                                                                              <  1:1432154/148‑1 (MQ=255)
  agaTTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGtt                                                                                                                                            <  2:2365520/148‑1 (MQ=255)
    aTTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTAc                                                                                                                                          >  1:2277371/1‑148 (MQ=255)
      tAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTTCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAg                                                                                                                                        <  2:780349/148‑1 (MQ=255)
       aaaCATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGa                                                                                                                                       <  2:3219762/148‑1 (MQ=255)
         aCATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACg                                                                                                                                     >  2:3171662/1‑148 (MQ=255)
          cATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACgg                                                                                                                                    >  1:252216/1‑148 (MQ=255)
           aTGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGgc                                                                                                                                   >  1:3272699/1‑148 (MQ=255)
            tGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGgcg                                                                                                                                  >  1:2619739/1‑148 (MQ=255)
            tGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGgcg                                                                                                                                  >  1:524741/1‑148 (MQ=255)
              aaaTCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCa                                                                                                                                >  1:2677365/1‑148 (MQ=255)
                aTCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGa                                                                                                                              >  1:2854744/1‑148 (MQ=255)
                 tCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGaa                                                                                                                             >  2:249311/1‑148 (MQ=255)
                     gCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACAcc                                                                                                                         <  2:2804831/148‑1 (MQ=255)
                     gCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACAcc                                                                                                                         <  1:344708/148‑1 (MQ=255)
                       tGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCTGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCAt                                                                                                                       <  2:543646/148‑1 (MQ=255)
                            cTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTc                                                                                                                  >  2:1918728/1‑148 (MQ=255)
                                   ccGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACtt                                                                                                           >  1:1132843/1‑148 (MQ=255)
                                   ccGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACtt                                                                                                           >  2:1713171/1‑148 (MQ=255)
                                       cAACCACGTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTGATCCGCCTTGTGTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACg                                                                                                       <  2:2129336/148‑1 (MQ=255)
                                        aaCCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGc                                                                                                      >  2:1882115/1‑148 (MQ=255)
                                          ccACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTg                                                                                                    >  2:3805325/1‑148 (MQ=255)
                                              tttCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGtt                                                                                                <  2:1264737/148‑1 (MQ=255)
                                              tttCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGtt                                                                                                <  2:3091281/148‑1 (MQ=255)
                                              tttCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGtt                                                                                                >  1:3037478/1‑148 (MQ=255)
                                               ttCTGCGTCAACAGCCAGATGCGCTGAAGGTGCAGTTAGCCGCCGTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGttt                                                                                               <  1:1479841/148‑1 (MQ=255)
                                               ttCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGttt                                                                                               <  2:497119/148‑1 (MQ=255)
                                               ttCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGttt                                                                                               >  2:3614958/1‑148 (MQ=255)
                                               ttCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGttt                                                                                               <  2:3694596/148‑1 (MQ=255)
                                                tCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGtttt                                                                                              >  2:1368358/1‑148 (MQ=255)
                                                   gCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGcc                                                                                           >  2:1419418/1‑148 (MQ=255)
                                                   gCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGcc                                                                                           >  1:2693422/1‑148 (MQ=255)
                                                    cGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCg                                                                                          <  2:3425795/148‑1 (MQ=255)
                                                           aGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGa                                                                                   <  2:2934027/148‑1 (MQ=255)
                                                            gCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGCGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGaa                                                                                  >  2:1342187/1‑148 (MQ=255)
                                                                gATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAATCGAGATTCCTACCCGCTGTGAGACTCTGTTACAGACGGCGCATAACACACTCAGTCTCGACTTTACGCTGCGTTTTGCCGAGCTTGAATcac                                                                              >  1:1412658/1‑146 (MQ=255)
                                                                    cgcTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACt                                                                                                            <  2:903735/114‑1 (MQ=255)
                                                                    cgcTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACt                                                                                                            >  1:903735/1‑114 (MQ=255)
                                                                    cgcTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCt                                                                          <  1:941632/148‑1 (MQ=255)
                                                                    cgcTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCt                                                                          <  1:328522/148‑1 (MQ=255)
                                                                        gAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGc                                                                      >  2:1769702/1‑148 (MQ=255)
                                                                         aaGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGcc                                                                     <  1:3249056/148‑1 (MQ=255)
                                                                            gTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCggg                                                                  <  1:844944/148‑1 (MQ=255)
                                                                              gCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGgtg                                                                <  2:737020/148‑1 (MQ=255)
                                                                               cAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGgtgt                                                               <  1:42120/148‑1 (MQ=255)
                                                                                 gTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTa                                                             <  1:1809810/148‑1 (MQ=255)
                                                                                 gTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTa                                                             <  1:30782/148‑1 (MQ=255)
                                                                                 gTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTa                                                             >  1:1167058/1‑148 (MQ=255)
                                                                                       cgccTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGAt                                                       <  2:635819/148‑1 (MQ=255)
                                                                                        gccTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATg                                                      <  1:869414/148‑1 (MQ=255)
                                                                                        gccTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATg                                                      >  1:1056722/1‑148 (MQ=255)
                                                                                        gccTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATg                                                      <  1:2037591/148‑1 (MQ=255)
                                                                                        gccTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATg                                                      >  1:2881795/1‑148 (MQ=255)
                                                                                         ccTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGt                                                     <  1:358862/148‑1 (MQ=255)
                                                                                         ccTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGt                                                     >  1:1277304/1‑148 (MQ=255)
                                                                                           ttGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTa                                                   <  1:2207551/148‑1 (MQ=255)
                                                                                            tGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCCTCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTAt                                                  >  2:2377481/1‑148 (MQ=255)
                                                                                            tGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTAt                                                  <  1:2493036/148‑1 (MQ=255)
                                                                                            tGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTAt                                                  <  1:2876086/148‑1 (MQ=255)
                                                                                                 cAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGcc                                             <  2:743860/148‑1 (MQ=255)
                                                                                                   ggATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGcccc                                           >  1:1649794/1‑148 (MQ=255)
                                                                                                    gatgatTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCa                                          <  1:3171662/148‑1 (MQ=255)
                                                                                                    gatgatTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCa                                          >  2:2111679/1‑148 (MQ=255)
                                                                                                     atgatTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCAt                                         >  1:2363000/1‑148 (MQ=255)
                                                                                                       gatTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTg                                       <  1:1882115/148‑1 (MQ=255)
                                                                                                       gatTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTg                                       >  1:2094260/1‑148 (MQ=255)
                                                                                                       gactCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTg                                       >  1:2288923/4‑148 (MQ=255)
                                                                                                            ggAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGcaa                                  <  2:3037478/148‑1 (MQ=255)
                                                                                                            ggAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGcaa                                  <  2:1653354/148‑1 (MQ=255)
                                                                                                               gCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGcaacaa                               >  1:3571408/1‑148 (MQ=255)
                                                                                                                ccGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAg                              >  2:359705/1‑148 (MQ=255)
                                                                                                                     aaGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCaaa                         <  1:3528743/148‑1 (MQ=255)
                                                                                                                     aaGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCaaa                         <  1:2167634/148‑1 (MQ=255)
                                                                                                                      aGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCaaaa                        >  2:1090887/1‑148 (MQ=255)
                                                                                                                       gCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAAt                       >  1:1693769/1‑148 (MQ=255)
                                                                                                                         gagaTTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATgg                     <  2:1673605/148‑1 (MQ=255)
                                                                                                                           gaTTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGaa                   <  2:408518/148‑1 (MQ=255)
                                                                                                                           gaTTCGTACCCGCTGTGAGACACTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGaa                   <  2:1405097/148‑1 (MQ=255)
                                                                                                                              tCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTg                >  2:2616351/1‑148 (MQ=255)
                                                                                                                              tCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTg                <  2:2881795/148‑1 (MQ=255)
                                                                                                                              tCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTg                <  2:3278514/148‑1 (MQ=255)
                                                                                                                               cGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGa               <  2:1712379/148‑1 (MQ=255)
                                                                                                                                gTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAg              >  1:1303801/1‑148 (MQ=255)
                                                                                                                                 tACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGc             <  2:1928504/148‑1 (MQ=255)
                                                                                                                                  aCCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCg            <  2:613185/148‑1 (MQ=255)
                                                                                                                                   cccGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGa           <  1:1455278/148‑1 (MQ=255)
                                                                                                                                   cccGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGa           <  2:2144677/148‑1 (MQ=255)
                                                                                                                                     cGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGt         <  1:1918728/148‑1 (MQ=255)
                                                                                                                                     cGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGt         >  2:2817411/1‑148 (MQ=255)
                                                                                                                                     cGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGt         <  1:3805325/148‑1 (MQ=255)
                                                                                                                                      gCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTc        <  1:2108584/148‑1 (MQ=255)
                                                                                                                                        tgtgAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAg      >  2:1672317/1‑148 (MQ=255)
                                                                                                                                         gtgAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAgg     <  2:192218/148‑1 (MQ=255)
                                                                                                                                          tgAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAggg    <  1:1713171/148‑1 (MQ=255)
                                                                                                                                          tgAGACACTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAggg    >  2:3460062/1‑148 (MQ=255)
                                                                                                                                           gagaCTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGt   <  2:3516514/148‑1 (MQ=255)
                                                                                                                                            agaCTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAACTGGACTTGAGCGAGTCAGGGtt  >  2:2029821/1‑148 (MQ=255)
                                                                                                                                            agaCTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGtt  >  1:1093229/1‑148 (MQ=255)
                                                                                                                                            agaCTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGtt  <  1:2070028/148‑1 (MQ=255)
                                                                                                                                            agaCTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGtt  >  1:2151201/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                               
CGAGATTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGTT  >  CP009273/4318373‑4318660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑