Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,350,223 G→A 100% A510V (GCT→GTT)  cadC ← cadBA operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,350,2230GA97.0% 1093.6 / 16.8 336A510V (GCT→GTT) cadCcadBA operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (2/8);  new base A (163/163);  total (165/171)
Fisher's exact test for biased strand distribution p-value = 1.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 336 total aligned reads displayed.

TGCGTGACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCA  >  CP009273/4350079‑4350367
                                                                                                                                                |                                                                                                                                                
tGCGTGACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACaa                                                                                                                                               >  2:1752685/1‑148 (MQ=255)
tGCGTGACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACaa                                                                                                                                               >  1:1421039/1‑148 (MQ=255)
  cGTGACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGa                                                                                                                                             <  1:1522235/148‑1 (MQ=255)
  cGTGACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGa                                                                                                                                             <  1:3421552/148‑1 (MQ=255)
  cGTGACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGa                                                                                                                                             <  1:3481632/148‑1 (MQ=255)
   gTGACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGaa                                                                                                                                            <  2:993967/148‑1 (MQ=255)
    tGACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACACGaaa                                                                                                                                           >  2:100249/1‑148 (MQ=255)
     gACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAAt                                                                                                                                          >  2:3724840/1‑148 (MQ=255)
     gACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCAGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAAt                                                                                                                                          >  2:2247150/1‑148 (MQ=255)
      aCATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAAtt                                                                                                                                         >  2:451934/1‑148 (MQ=255)
      aCATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAAtt                                                                                                                                         >  1:3351387/1‑148 (MQ=255)
        aTTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTg                                                                                                                                       <  1:2278617/148‑1 (MQ=255)
            tctGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCga                                                                                                                                   <  1:169751/148‑1 (MQ=255)
             ctGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCgag                                                                                                                                  <  2:160146/148‑1 (MQ=255)
                 ttAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATaa                                                                                                                              <  2:376960/148‑1 (MQ=255)
                 ttAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATaa                                                                                                                              <  2:2292125/148‑1 (MQ=255)
                     aTTTATGTAATAAAAATTATGCGGCAAATAACTTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTa                                                                                                                          >  1:3106636/1‑148 (MQ=255)
                      tttATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTac                                                                                                                         >  2:768523/1‑148 (MQ=255)
                       ttATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTaca                                                                                                                        <  1:2513003/148‑1 (MQ=255)
                        tATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTacaa                                                                                                                       <  1:2140394/148‑1 (MQ=255)
                             aataaAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATaa                                                                                                                  <  1:3732969/148‑1 (MQ=255)
                               taaAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAgg                                                                                                                >  2:1270120/1‑148 (MQ=255)
                               taaAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAgg                                                                                                                >  1:1878001/1‑148 (MQ=255)
                               taaAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAgg                                                                                                                >  1:1109662/1‑148 (MQ=255)
                                aaaaaTTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATAAGGTACAACATAAGGa                                                                                                               <  2:3454834/148‑1 (MQ=255)
                                aaaaaTTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGa                                                                                                               >  1:3216784/1‑148 (MQ=255)
                                aaaaaTTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGa                                                                                                               >  1:890850/1‑148 (MQ=255)
                                aaaaaTTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGa                                                                                                               <  1:2362853/148‑1 (MQ=255)
                                    aTTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAg                                                                                                           <  1:3161119/148‑1 (MQ=255)
                                    aTTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAg                                                                                                           >  2:2467192/1‑148 (MQ=255)
                                     ttATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGa                                                                                                          <  1:2781927/148‑1 (MQ=255)
                                     ttATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGa                                                                                                          <  2:967642/148‑1 (MQ=255)
                                      tATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGaa                                                                                                         <  2:1485050/148‑1 (MQ=255)
                                        tGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGt                                                                                                       <  2:3430773/148‑1 (MQ=255)
                                         gCGGCAAATAAATTGCCGCAACATATTATACCAACATGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTc                                                                                                      <  2:718684/148‑1 (MQ=255)
                                          cGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATTAGGTACAACATAAGGAACAGAAGTCt                                                                                                     <  2:3094734/148‑1 (MQ=255)
                                               aataaatTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAg                                                                                                        <  1:105975/140‑1 (MQ=255)
                                               aataaatTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAat                                                                                                <  1:662425/148‑1 (MQ=255)
                                                ataaatTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAata                                                                                               >  2:3359034/1‑148 (MQ=255)
                                                  aaatTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGACATTTGTAGAGATAAGGTACAACATAAGGAACATAAGTCTGGAAtata                                                                                             >  2:2444904/1‑148 (MQ=255)
                                                  aaatTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAAtata                                                                                             <  2:1984885/148‑1 (MQ=255)
                                                     ttGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCa                                                                                          >  1:539063/1‑148 (MQ=255)
                                                     ttGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCa                                                                                          >  1:2061445/1‑148 (MQ=255)
                                                      tGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCAt                                                                                         <  2:2748415/148‑1 (MQ=255)
                                                        ccGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCAttt                                                                                       <  1:198896/148‑1 (MQ=255)
                                                        ccGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCAttt                                                                                       <  1:970909/148‑1 (MQ=255)
                                                           cAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCa                                                                                    >  1:1325356/1‑148 (MQ=255)
                                                             aCATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAAt                                                                                  >  1:2994126/1‑148 (MQ=255)
                                                              cATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATc                                                                                 <  2:2100554/148‑1 (MQ=255)
                                                                tattatACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCa                                                                               >  2:2603214/1‑148 (MQ=255)
                                                                tattatACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCa                                                                               >  2:3591137/1‑148 (MQ=255)
                                                                tattatACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCa                                                                               >  2:2955512/1‑148 (MQ=255)
                                                                     taCCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTaaa                                                                          <  2:2782241/148‑1 (MQ=255)
                                                                        cAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAggg                                                                       <  2:862596/148‑1 (MQ=255)
                                                                        cAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAggg                                                                       >  1:1234564/1‑148 (MQ=255)
                                                                           cAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGgtgt                                                                    >  1:2299685/1‑148 (MQ=255)
                                                                             ggAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGttt                                                                  >  2:1888178/1‑148 (MQ=255)
                                                                                aCATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGcc                                                               >  1:3773579/1‑148 (MQ=255)
                                                                                 cATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGccc                                                              <  1:2161537/148‑1 (MQ=255)
                                                                                 cATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGccc                                                              <  1:3490656/148‑1 (MQ=255)
                                                                                    aCAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTg                                                           >  2:1976186/1‑148 (MQ=255)
                                                                                    aCAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTg                                                           >  1:1644704/1‑148 (MQ=255)
                                                                                    aCAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTg                                                           <  1:3632239/148‑1 (MQ=255)
                                                                                      aaaaaCTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTggg                                                         >  1:2875083/1‑148 (MQ=255)
                                                                                        aaaCTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCg                                                       <  2:1817977/148‑1 (MQ=255)
                                                                                         aaCTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGt                                                      <  1:2694233/148‑1 (MQ=255)
                                                                                          aCTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTa                                                     >  1:1365035/1‑148 (MQ=255)
                                                                                           cTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTAATTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACAATTTTCAATCCAGTAAAGGGTGTTTGCCCATGGGCGTaa                                                    >  1:2755958/1‑148 (MQ=255)
                                                                                            tCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTaaa                                                   <  1:1311072/148‑1 (MQ=255)
                                                                                                caacaaATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTaa                                               >  2:3580685/1‑148 (MQ=255)
                                                                                                caacaaATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTaa                                               <  2:677539/148‑1 (MQ=255)
                                                                                                caacaaATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTaa                                               <  1:3702344/148‑1 (MQ=255)
                                                                                                caacaaATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTaa                                               <  2:2669509/148‑1 (MQ=255)
                                                                                                 aacaaATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTaaa                                              <  2:3197398/148‑1 (MQ=255)
                                                                                                 aacaaATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTaaa                                              <  2:166607/148‑1 (MQ=255)
                                                                                                  acaaATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCAAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAg                                             >  1:3542056/1‑148 (MQ=255)
                                                                                                     aaTATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCg                                          >  1:1792645/1‑148 (MQ=255)
                                                                                                       tatTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGt                                        >  1:1016233/1‑148 (MQ=255)
                                                                                                        atTTCCGAGCATAAATCAACCCGGAGATACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTg                                       >  2:680402/1‑148 (MQ=255)
                                                                                                          ttCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTgag                                     <  1:197621/148‑1 (MQ=255)
                                                                                                           tCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTgaga                                    <  1:3595080/148‑1 (MQ=255)
                                                                                                           tCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTgaga                                    <  2:3684190/148‑1 (MQ=255)
                                                                                                            ccGAGCATAAATCAACCCGGAGTTACTTATTCTTAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGat                                   <  2:439074/148‑1 (MQ=255)
                                                                                                            ccGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGat                                   <  2:1055874/148‑1 (MQ=255)
                                                                                                            ccGAGCATAAATCAACCAGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGat                                   <  1:3739626/148‑1 (MQ=255)
                                                                                                                 cATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCa                              >  1:2656321/1‑148 (MQ=255)
                                                                                                                 cATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCa                              <  2:3287778/148‑1 (MQ=255)
                                                                                                                      aTCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAgc                         >  1:3243851/1‑148 (MQ=255)
                                                                                                                      aTCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAgc                         <  2:3005110/148‑1 (MQ=255)
                                                                                                                      aTCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAgc                         >  1:427883/1‑148 (MQ=255)
                                                                                                                           cccGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCtt                    <  2:2153925/148‑1 (MQ=255)
                                                                                                                             cGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTcc                  <  1:14238/148‑1 (MQ=255)
                                                                                                                             cGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTcc                  <  2:604750/148‑1 (MQ=255)
                                                                                                                                  ttACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGtt             >  1:599048/1‑148 (MQ=255)
                                                                                                                                  ttACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGtt             >  1:3334529/1‑148 (MQ=255)
                                                                                                                                  ttACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGtt             >  2:3290471/1‑148 (MQ=255)
                                                                                                                                   tACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTc            >  2:3537777/1‑148 (MQ=255)
                                                                                                                                   tACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTc            >  1:3339210/1‑148 (MQ=255)
                                                                                                                                   tACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTc            >  2:117021/1‑148 (MQ=255)
                                                                                                                                      ttATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATc         >  2:3500080/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TGCGTGACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCA  >  CP009273/4350079‑4350367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑