Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,366,322 A→C 100% intergenic (+63/‑48) ecnA → / → ecnB entericidin A membrane lipoprotein, antidote entericidin B/entericidin B membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,366,3220AC99.0% 808.4 / ‑3.8 202intergenic (+63/‑48)ecnA/ecnBentericidin A membrane lipoprotein, antidote entericidin B/entericidin B membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base C (97/103);  total (97/105)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 209 total aligned reads displayed.

TGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTT  >  CP009273/4366177‑4366466
                                                                                                                                                 |                                                                                                                                                
tgctCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCGTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCAt                                                                                                                                                <  2:2532761/148‑1 (MQ=255)
    cACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGc                                                                                                                                            <  2:2167728/148‑1 (MQ=255)
     aCGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCt                                                                                                                                           <  2:1084423/148‑1 (MQ=255)
       gggCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCtat                                                                                                                                         <  1:1163709/148‑1 (MQ=255)
       gggCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCtat                                                                                                                                         <  2:1835533/148‑1 (MQ=255)
          cTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAgg                                                                                                                                      >  2:2021057/1‑148 (MQ=255)
           tGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGc                                                                                                                                     >  2:3402126/1‑148 (MQ=255)
              aaCACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCaaa                                                                                                                                  <  1:3667131/148‑1 (MQ=255)
               acacCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAAc                                                                                                                                 <  2:517374/148‑1 (MQ=255)
               acacCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAAc                                                                                                                                 >  1:1557618/1‑148 (MQ=255)
               acacCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAAc                                                                                                                                 <  2:1907976/148‑1 (MQ=255)
               acacCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAAc                                                                                                                                 <  2:1907478/148‑1 (MQ=255)
                cacCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACa                                                                                                                                <  1:3010298/148‑1 (MQ=255)
                   cGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACataa                                                                                                                             >  2:1901552/1‑148 (MQ=255)
                   cGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACataa                                                                                                                             <  2:463717/148‑1 (MQ=255)
                      tCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACataaata                                                                                                                          <  2:905617/148‑1 (MQ=255)
                      tCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTGTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACataaata                                                                                                                          <  2:3243268/148‑1 (MQ=255)
                       cgcgGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACataaataa                                                                                                                         >  2:1229311/1‑148 (MQ=255)
                         cgGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACa                                                                                                                       <  2:2029664/148‑1 (MQ=255)
                          ggTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACAt                                                                                                                      <  1:3730997/148‑1 (MQ=255)
                          ggTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGCCTATTATTCATTTGCTATAGGCAAACATAAATAACAt                                                                                                                      <  2:629056/148‑1 (MQ=255)
                           gTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACAtt                                                                                                                     <  1:1000546/148‑1 (MQ=255)
                           gTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACAtt                                                                                                                     <  1:3317730/148‑1 (MQ=255)
                           gTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACAtt                                                                                                                     <  1:1415758/148‑1 (MQ=255)
                            tttCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTa                                                                                                                    <  2:337782/148‑1 (MQ=255)
                              tCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTAcc                                                                                                                  <  1:1101224/148‑1 (MQ=255)
                                 gCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTaa                                                                                                               <  1:2683594/148‑1 (MQ=255)
                                  cGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTaaa                                                                                                              <  2:10723/148‑1 (MQ=255)
                                    aaGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAg                                                                                                            <  2:3221388/148‑1 (MQ=255)
                                       aCATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGaa                                                                                                         >  2:3361201/1‑148 (MQ=255)
                                        cATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAg                                                                                                        >  1:2500227/1‑148 (MQ=255)
                                          tCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGAc                                                                                                      >  2:2073974/1‑148 (MQ=255)
                                           cAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACg                                                                                                     >  2:1257609/1‑148 (MQ=255)
                                           cAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACg                                                                                                     <  2:732501/148‑1 (MQ=255)
                                             aaCATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGtt                                                                                                   >  2:2646982/1‑148 (MQ=255)
                                              aCATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTa                                                                                                  >  2:3539429/1‑148 (MQ=255)
                                              aCATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTa                                                                                                  <  2:1141399/148‑1 (MQ=255)
                                               cATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTAt                                                                                                 <  2:884412/148‑1 (MQ=255)
                                                 tctcGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATgg                                                                                               >  1:1720759/1‑148 (MQ=255)
                                                  ctcGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGt                                                                                              >  1:3116330/1‑148 (MQ=255)
                                                    cGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTga                                                                                            >  1:1061254/1‑148 (MQ=255)
                                                     ggCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTgaa                                                                                           >  2:2138839/1‑148 (MQ=255)
                                                      gCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTgaag                                                                                          >  1:2827468/1‑148 (MQ=255)
                                                      gCAACTCCATCTCTCGCGCGGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTgaag                                                                                          <  2:148174/148‑1 (MQ=255)
                                                             cATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACaa                                                                                   <  2:2461288/148‑1 (MQ=255)
                                                             cATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACaa                                                                                   <  2:1303202/148‑1 (MQ=255)
                                                                ctctcGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTg                                                                                >  1:491543/1‑148 (MQ=255)
                                                                 tctcGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGc                                                                               >  2:3766341/1‑148 (MQ=255)
                                                                 tctcGCACTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGc                                                                               >  2:2849765/1‑148 (MQ=255)
                                                                  ctcGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCa                                                                              >  2:1719192/1‑148 (MQ=255)
                                                                   tcGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAg                                                                             <  2:504800/148‑1 (MQ=255)
                                                                    cgcgCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGc                                                                            <  1:2021060/148‑1 (MQ=255)
                                                                       gcTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGAt                                                                         <  1:1307629/148‑1 (MQ=255)
                                                                         tGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCt                                                                       <  1:45334/148‑1 (MQ=255)
                                                                          gCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCtt                                                                      >  1:3082088/1‑148 (MQ=255)
                                                                             aGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTttc                                                                   <  1:688298/148‑1 (MQ=255)
                                                                             aGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTttc                                                                   <  1:690543/148‑1 (MQ=255)
                                                                                 aaTTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTttctgtt                                                               >  1:741293/1‑148 (MQ=255)
                                                                                  aTTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTttctgttc                                                              >  1:3601248/1‑148 (MQ=255)
                                                                                   tttttCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTttctgttct                                                             >  1:2476682/1‑148 (MQ=255)
                                                                                   tttttCTTCTCTTACGAAAAATCATCAGATTCCCATCATTTTTGGCTATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACATAAAAGGAAGAACTTATTGTGAAGAAGACAATTGCAGCGATcttttcttttat                                                             >  2:3138498/1‑146 (MQ=255)
                                                                                    ttttCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTttctgttctg                                                            <  1:3035336/148‑1 (MQ=255)
                                                                                    ttttCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTttctgttctg                                                            >  2:1833428/1‑148 (MQ=255)
                                                                                    ttttCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACCTTATGGTGAAGAAGACAATTGCAGCGATCTTttctgttctg                                                            >  2:207965/1‑148 (MQ=255)
                                                                                     tttCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTgg                                                           <  1:2782979/148‑1 (MQ=255)
                                                                                     tttCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTgg                                                           >  1:3369004/1‑148 (MQ=255)
                                                                                       tcttctcttcCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTg                                                         <  1:1553666/148‑1 (MQ=255)
                                                                                        cttctcttcCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGc                                                        >  1:1707170/1‑148 (MQ=255)
                                                                                         ttctcttcCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCt                                                       <  1:1883244/148‑1 (MQ=255)
                                                                                                   aaaaaTCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTa                                             >  1:2771250/1‑148 (MQ=255)
                                                                                                   aaaaaTCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTa                                             >  1:857217/1‑148 (MQ=255)
                                                                                                     aaaTCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTAtt                                           >  1:1339879/1‑148 (MQ=255)
                                                                                                      aaTCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTa                                          >  2:3536348/1‑148 (MQ=255)
                                                                                                      aaTCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTa                                          >  2:2904837/1‑148 (MQ=255)
                                                                                                       atcatcAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTaa                                         >  2:2676885/1‑148 (MQ=255)
                                                                                                       atcatcAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTaa                                         <  2:2500227/148‑1 (MQ=255)
                                                                                                         catcaGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAAct                                       >  1:2219955/1‑148 (MQ=255)
                                                                                                           tcaGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAActgc                                     >  2:3739924/1‑148 (MQ=255)
                                                                                                              gATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAActgcctg                                  >  2:2989713/1‑148 (MQ=255)
                                                                                                              gATTACCATCAAGATTGGCGATGTAGTATATTATTCATTTGCTAAACGAAAACATAAATAACAATACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGATATGGTGCTTTAAACAGTATTAActgcctg                                  >  2:2342870/1‑148 (MQ=255)
                                                                                                                 tCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCaa                               >  1:705245/1‑148 (MQ=255)
                                                                                                                     atcatTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAAcacc                           <  2:844994/148‑1 (MQ=255)
                                                                                                                      tcatTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAAcacca                          >  2:828609/1‑148 (MQ=255)
                                                                                                                      tcatTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAAcacca                          >  1:2918222/1‑148 (MQ=255)
                                                                                                                       catTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAAcaccac                         >  2:3248670/1‑148 (MQ=255)
                                                                                                                         tttttGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCAcgc                       >  1:3797876/1‑148 (MQ=255)
                                                                                                                            ttGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTg                    <  1:2138839/148‑1 (MQ=255)
                                                                                                                               gCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCg                 <  1:991422/148‑1 (MQ=255)
                                                                                                                                   tgttgtCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTgg             >  1:1553618/1‑148 (MQ=255)
                                                                                                                                   tgttgtCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTgg             <  1:1833428/148‑1 (MQ=255)
                                                                                                                                     ttgtCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTg           >  1:2019194/1‑148 (MQ=255)
                                                                                                                                       gtCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGaa         >  1:1941908/1‑148 (MQ=255)
                                                                                                                                        tCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAg        <  2:3356476/148‑1 (MQ=255)
                                                                                                                                          tattatTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGAc      >  2:532754/1‑148 (MQ=255)
                                                                                                                                           attattCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACa     <  1:2073974/148‑1 (MQ=255)
                                                                                                                                           attattCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACa     >  2:2305628/1‑148 (MQ=255)
                                                                                                                                             tattcATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACAtt   >  1:233390/1‑148 (MQ=255)
                                                                                                                                             tattcATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACAtt   >  1:992532/1‑148 (MQ=255)
                                                                                                                                              attcATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACAttt  <  2:3488408/148‑1 (MQ=255)
                                                                                                                                              attcATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACAttt  <  2:79726/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTT  >  CP009273/4366177‑4366466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑