Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,376,178 C→A 100% E1004* (GAA→TAA)  mscM ← mechanosensitive channel protein, miniconductance

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,376,1780CA98.1% 690.3 / 3.3 220E1004* (GAA→TAA) mscMmechanosensitive channel protein, miniconductance
Reads supporting (aligned to +/- strand):  ref base C (2/3);  new base A (107/107);  total (109/111)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 223 total aligned reads displayed.

GTGACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTCGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTGATGGTGGTG  >  CP009273/4376035‑4376320
                                                                                                                                               |                                                                                                                                              
 tGACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTAt                                                                                                                                           <  1:2027943/148‑1 (MQ=255)
     ccATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGc                                                                                                                                       <  2:2014796/148‑1 (MQ=255)
     ccATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGc                                                                                                                                       <  1:3635159/148‑1 (MQ=255)
     ccATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGc                                                                                                                                       <  2:338452/148‑1 (MQ=255)
      cATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTCGCTATTGGCa                                                                                                                                      <  2:707547/148‑1 (MQ=255)
      cATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTCGCTATTGGCa                                                                                                                                      <  2:396408/148‑1 (MQ=255)
      cATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCa                                                                                                                                      >  1:3142018/1‑148 (MQ=255)
        tctcAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATc                                                                                                                                    >  1:24564/1‑148 (MQ=255)
             gcggcgTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAg                                                                                                                               <  1:622839/148‑1 (MQ=255)
             gcggcgTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAg                                                                                                                               <  1:1836614/148‑1 (MQ=255)
              cggcgTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAgg                                                                                                                              <  1:3350564/148‑1 (MQ=255)
              cggcgTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAgg                                                                                                                              >  2:2922335/1‑148 (MQ=255)
                   tAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCg                                                                                                                         >  1:993941/1‑148 (MQ=255)
                    aaaTACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCgg                                                                                                                        >  1:3563303/1‑148 (MQ=255)
                    aaaTACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCgg                                                                                                                        <  1:23964/148‑1 (MQ=255)
                      aTACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTa                                                                                                                      >  2:139592/1‑148 (MQ=255)
                       tACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTAt                                                                                                                     >  1:1306603/1‑148 (MQ=255)
                        aCGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTAta                                                                                                                    >  2:3392005/1‑147 (MQ=255)
                             gCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCaa                                                                                                               >  1:2672944/1‑148 (MQ=255)
                                cGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAacac                                                                                                            >  1:968324/1‑148 (MQ=255)
                                     aTCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCAcgc                                                                                                       >  1:1749837/1‑148 (MQ=255)
                                     aTCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCAcgc                                                                                                       >  1:772519/1‑148 (MQ=255)
                                      tCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTCGCTATTGGCATCGGCAGGGGCCGGTCTCGTCAACACCAcgcg                                                                                                      >  2:679680/1‑148 (MQ=255)
                                       cTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCAcgcgc                                                                                                     >  1:2831462/1‑148 (MQ=255)
                                         gAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGt                                                                                                   <  2:2668649/148‑1 (MQ=255)
                                          aaTCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTg                                                                                                  >  1:1436260/1‑148 (MQ=255)
                                           aTCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGa                                                                                                 >  2:101031/1‑148 (MQ=255)
                                            tCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGAc                                                                                                >  1:916486/1‑148 (MQ=255)
                                             ccccTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGAcc                                                                                               >  1:1426544/1‑148 (MQ=255)
                                              cccTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCg                                                                                              >  1:2614744/1‑148 (MQ=255)
                                              cccTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCg                                                                                              <  2:3105556/148‑1 (MQ=255)
                                              cccTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCg                                                                                              <  1:1948700/148‑1 (MQ=255)
                                               ccTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGa                                                                                             <  1:1235191/148‑1 (MQ=255)
                                               ccTGTTGCAGATCCACCAGGAAGACGTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGa                                                                                             <  2:3662208/148‑1 (MQ=255)
                                                cTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAg                                                                                            >  2:1162237/1‑148 (MQ=255)
                                                 tGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGt                                                                                           >  1:1045221/1‑148 (MQ=255)
                                                   ttGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCa                                                                                         >  1:1917223/1‑148 (MQ=255)
                                                    tGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCag                                                                                        <  1:2140258/148‑1 (MQ=255)
                                                      cAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCagag                                                                                      >  2:1813063/1‑148 (MQ=255)
                                                        gATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCagagag                                                                                    >  2:353655/1‑148 (MQ=255)
                                                        gATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCagagag                                                                                    >  2:3298543/1‑148 (MQ=255)
                                                          tCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTCTTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCg                                                                                  >  2:357302/1‑148 (MQ=255)
                                                          tCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCg                                                                                  >  2:2477725/1‑148 (MQ=255)
                                                          tCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCg                                                                                  >  1:931516/1‑148 (MQ=255)
                                                            caccaGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGAc                                                                                <  1:2876635/148‑1 (MQ=255)
                                                            caccaGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGAc                                                                                >  1:3506634/1‑148 (MQ=255)
                                                             accaGGAAGACTTCGGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCTGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGAcc                                                                               <  1:1128455/148‑1 (MQ=255)
                                                             accaGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGAcc                                                                               <  2:3677138/148‑1 (MQ=255)
                                                                aGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGt                                                                            >  2:1540725/1‑148 (MQ=255)
                                                                  gAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTg                                                                          >  1:491145/1‑148 (MQ=255)
                                                                  gAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTg                                                                          >  1:490927/1‑148 (MQ=255)
                                                                  gAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTg                                                                          >  2:781498/1‑148 (MQ=255)
                                                                   aaGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTTGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGGGACCGAGTCAGAGAGCGACCAGTTGa                                                                         <  1:2941910/148‑1 (MQ=255)
                                                                   aaGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTCTTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGa                                                                         >  1:3704690/1‑148 (MQ=255)
                                                                   aaGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGa                                                                         <  2:650465/148‑1 (MQ=255)
                                                                   aaGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGa                                                                         >  1:1775086/1‑148 (MQ=255)
                                                                   aaGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGa                                                                         <  1:1571510/148‑1 (MQ=255)
                                                                          ccGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGGGACCGAGTCAGAGAGCGACCAGTTGATAAACTg                                                                  <  2:1652433/148‑1 (MQ=255)
                                                                           cGGGGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGc                                                                 <  2:2832349/148‑1 (MQ=255)
                                                                              tGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCg                                                              >  1:3322419/1‑148 (MQ=255)
                                                                                 cGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGCGTCAGAGAGCGACCAGTTGATAAACTGCTCGGta                                                           <  1:3227342/148‑1 (MQ=255)
                                                                                 cGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGAGACCAGTTGATAAACTGCTCGGta                                                           <  1:1065194/148‑1 (MQ=255)
                                                                                  gggTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGtaa                                                          <  2:3191787/148‑1 (MQ=255)
                                                                                     ttGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGtaataa                                                       <  2:3480568/148‑1 (MQ=255)
                                                                                          gATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGcc                                                  <  2:2515860/148‑1 (MQ=255)
                                                                                           aTCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCt                                                 <  2:96160/148‑1 (MQ=255)
                                                                                           aTCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCt                                                 <  1:964844/148‑1 (MQ=255)
                                                                                               ccAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGtt                                             >  2:2693741/1‑148 (MQ=255)
                                                                                                      gcagcGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACg                                      <  2:931516/148‑1 (MQ=255)
                                                                                                      gcagcGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACg                                      >  2:51040/1‑148 (MQ=255)
                                                                                                      gcagcGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACg                                      >  2:3231426/1‑148 (MQ=255)
                                                                                                       cagcGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGa                                     >  2:689118/1‑148 (MQ=255)
                                                                                                        agcGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGAt                                    <  2:234246/148‑1 (MQ=255)
                                                                                                         gcGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGAtt                                   <  2:2392593/148‑1 (MQ=255)
                                                                                                           gACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTAt                                 <  2:415501/148‑1 (MQ=255)
                                                                                                           gACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTAt                                 <  2:415916/148‑1 (MQ=255)
                                                                                                            aCGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTAtc                                >  2:30284/1‑148 (MQ=255)
                                                                                                            aCGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTAtc                                >  2:1274943/1‑148 (MQ=255)
                                                                                                            aCGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTAtc                                >  2:227050/1‑148 (MQ=255)
                                                                                                               cgcTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATctct                             >  2:435274/1‑148 (MQ=255)
                                                                                                               cgcTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATctct                             <  2:1775086/148‑1 (MQ=255)
                                                                                                               cgcTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATctct                             <  1:2477725/148‑1 (MQ=255)
                                                                                                               cgcTGCGGTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATctct                             <  1:3092638/148‑1 (MQ=255)
                                                                                                                      gTGAGCAGGATTTCCGTCACTTCTTAGCTTTTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGt                      <  1:3208914/148‑1 (MQ=255)
                                                                                                                      gTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGt                      <  2:2206943/148‑1 (MQ=255)
                                                                                                                      gTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGt                      <  1:2696955/148‑1 (MQ=255)
                                                                                                                      gTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGt                      <  1:3008787/148‑1 (MQ=255)
                                                                                                                      gTGAGCAGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGt                      <  1:3759807/148‑1 (MQ=255)
                                                                                                                        gAGCAGGATTTCCGTCACTTCTTAGCTATGGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTcc                    <  1:1376419/148‑1 (MQ=255)
                                                                                                                        gAGCAGGAGTTCCGTCACTTCCTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTcc                    <  1:3280866/148‑1 (MQ=255)
                                                                                                                            aGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTTTGTCTTTACGGTCCCAGt                <  1:1773817/148‑1 (MQ=255)
                                                                                                                            aGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGt                <  1:3661891/148‑1 (MQ=255)
                                                                                                                            aGGATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGt                <  1:139592/148‑1 (MQ=255)
                                                                                                                              gATTTCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCg              <  2:1857447/148‑1 (MQ=255)
                                                                                                                                tttCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCt            >  2:2546585/1‑148 (MQ=255)
                                                                                                                                 ttCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTg           <  2:2068928/148‑1 (MQ=255)
                                                                                                                                  tCCGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTGa          <  2:1779963/148‑1 (MQ=255)
                                                                                                                                    cGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTGAtg        <  2:3142018/148‑1 (MQ=255)
                                                                                                                                    cGTCACTTCTTAGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTGAtg        <  2:855559/148‑1 (MQ=255)
                                                                                                                                          ttcttaGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTGAtggtggtg  >  2:1357398/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                              
GTGACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGAAGACTTCCGGTGCCGGGTTGTCGATCACCAGCGAGCAGCGACGCGCTGCGGTGAGCAGGATTTCCGTCACTTCTTCGCTATTGGCATCGGCAGGGGCCGGTATCGTCAACACCACGCGCGTGACCGAGTCAGAGAGCGACCAGTTGATAAACTGCTCGGTAATAAACGCCTTGTTCGGCACGATTATCTCTTTACGGTCCCAGTCGCTGATGGTGGTG  >  CP009273/4376035‑4376320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑