Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,378,778 G→T 100% S137* (TCG→TAG)  mscM ← mechanosensitive channel protein, miniconductance

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,378,7780GT98.9% 567.2 / ‑1.6 174S137* (TCG→TAG) mscMmechanosensitive channel protein, miniconductance
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (83/88);  total (85/89)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 174 total aligned reads displayed.

CGCCTTAAGACGTGCAGAGTCAGACTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCGAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAGCGCGTCGGTAGACATTCCCGGCGACACGCTGCGCGGCTCGTCACGCATGT  >  CP009273/4378633‑4378922
                                                                                                                                                 |                                                                                                                                                
cgcCTTAAGACGTGCAGAGTCAGACTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTaa                                                                                                                                                <  1:1510652/148‑1 (MQ=255)
 gcCTTAAGACGTGCAGAGTCAGACTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAAt                                                                                                                                               <  2:1500788/148‑1 (MQ=255)
        gACGTGCAGAGTCAGACTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCCTTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAAt                                                                                                                                        <  1:717599/148‑1 (MQ=255)
        gACGTGCAGAGTCAGACTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCCTTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAAt                                                                                                                                        <  1:432565/148‑1 (MQ=255)
        gACGTGCAGAGTCAGACTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAAt                                                                                                                                        <  1:717600/148‑1 (MQ=255)
        gACGTGCAGAGTCAGACTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAAt                                                                                                                                        <  1:3540909/148‑1 (MQ=255)
        gACGTGCAGAGTCAGACTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAAt                                                                                                                                        <  1:3465046/148‑1 (MQ=255)
        gACGTGCAGAGTCAGACTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAAt                                                                                                                                        <  2:2788231/148‑1 (MQ=255)
        gACGTGCAGAGTCAGACTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAAt                                                                                                                                        <  1:1852078/148‑1 (MQ=255)
        gACGTGCAGAGTCAGACTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAAt                                                                                                                                        >  2:721676/1‑148 (MQ=255)
         aCGTGCAGAGTCAGACTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAAtc                                                                                                                                       >  2:3525104/1‑148 (MQ=255)
             gCAGAGTCAGACTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTcgc                                                                                                                                   >  2:1416695/1‑148 (MQ=255)
              cAGAGTCAGACTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTcgcg                                                                                                                                  <  1:110468/148‑1 (MQ=255)
                   tCAGACTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGCCAATCTCGCGGgcgc                                                                                                                             >  2:3659159/1‑148 (MQ=255)
                    cAGACTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGgcgcg                                                                                                                            <  1:1718524/148‑1 (MQ=255)
                     aGACTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGgcgcgc                                                                                                                           <  2:874289/148‑1 (MQ=255)
                       aCTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTc                                                                                                                         >  1:2396921/1‑148 (MQ=255)
                       aCTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTc                                                                                                                         <  2:814140/148‑1 (MQ=255)
                       aCTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTc                                                                                                                         >  1:1770719/1‑148 (MQ=255)
                        cTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTcc                                                                                                                        <  2:2674904/148‑1 (MQ=255)
                        cTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTcc                                                                                                                        <  2:2674948/148‑1 (MQ=255)
                         tGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCt                                                                                                                       >  2:726989/1‑148 (MQ=255)
                         tGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCt                                                                                                                       >  2:103455/1‑148 (MQ=255)
                             aCGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCCTTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGctc                                                                                                                   <  1:1883100/148‑1 (MQ=255)
                                cgAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTg                                                                                                                <  2:3811247/148‑1 (MQ=255)
                                 gAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGc                                                                                                               <  2:1705393/148‑1 (MQ=255)
                                  aaaTTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCCTTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCt                                                                                                              <  1:1890362/148‑1 (MQ=255)
                                  aaaTTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCt                                                                                                              <  1:2992389/148‑1 (MQ=255)
                                   aaTTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTg                                                                                                             <  1:1479710/148‑1 (MQ=255)
                                   aaTTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTg                                                                                                             <  1:1365790/148‑1 (MQ=255)
                                    aTTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTgg                                                                                                            <  2:477580/148‑1 (MQ=255)
                                     ttttGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTggg                                                                                                           >  2:2605078/1‑148 (MQ=255)
                                       ttGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGcc                                                                                                         <  1:3567273/148‑1 (MQ=255)
                                        tgtgCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCt                                                                                                        >  2:3189059/1‑148 (MQ=255)
                                             cTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCCTTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACgg                                                                                                   <  1:2639929/148‑1 (MQ=255)
                                             cTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCCTTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACgg                                                                                                   <  1:949391/148‑1 (MQ=255)
                                             cTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACgg                                                                                                   >  1:2758004/1‑148 (MQ=255)
                                                aTTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCtt                                                                                                >  2:2941972/1‑148 (MQ=255)
                                                aTTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCGGGGCCTGACGGCtt                                                                                                >  1:1052947/1‑148 (MQ=255)
                                                    aGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTAt                                                                                            >  2:702077/1‑148 (MQ=255)
                                                    aGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTAt                                                                                            <  2:955455/148‑1 (MQ=255)
                                                    aGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTAt                                                                                            >  1:3707521/1‑148 (MQ=255)
                                                    aGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTAt                                                                                            >  1:340194/1‑148 (MQ=255)
                                                     gCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATc                                                                                           <  2:3093301/148‑1 (MQ=255)
                                                      cGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATcc                                                                                          >  2:2999941/1‑148 (MQ=255)
                                                      cGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATcc                                                                                          <  1:3636218/148‑1 (MQ=255)
                                                      cGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATcc                                                                                          >  2:3260601/1‑148 (MQ=255)
                                                      cGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTGTTATcc                                                                                          >  2:2010779/1‑148 (MQ=255)
                                                           tagtGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCaa                                                                                     <  2:1863812/145‑1 (MQ=255)
                                                           tATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCaa                                                                                     >  1:1904174/1‑148 (MQ=255)
                                                           tATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCaa                                                                                     <  2:1863889/148‑1 (MQ=255)
                                                               gCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCCTTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTgg                                                                                 <  1:2212216/148‑1 (MQ=255)
                                                               gCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTgg                                                                                 >  2:793952/1‑148 (MQ=255)
                                                                  ggTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGgctg                                                                              <  1:618952/148‑1 (MQ=255)
                                                                     aaGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGctgctg                                                                           <  2:598086/148‑1 (MQ=255)
                                                                     aaGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGctgctg                                                                           >  2:1273312/1‑148 (MQ=255)
                                                                     aaGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGctgctg                                                                           >  2:1273092/1‑148 (MQ=255)
                                                                      aGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGa                                                                          >  2:143006/1‑148 (MQ=255)
                                                                      aGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACAGGCTGCTGa                                                                          >  1:2724482/1‑148 (MQ=255)
                                                                       gCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGAc                                                                         <  1:3539151/148‑1 (MQ=255)
                                                                       gCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGAc                                                                         <  1:2893247/148‑1 (MQ=255)
                                                                        ctttCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGAcc                                                                        <  1:3184679/146‑1 (MQ=255)
                                                                         gTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCt                                                                       <  1:2813227/148‑1 (MQ=255)
                                                                           tCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTgg                                                                     >  2:292115/1‑148 (MQ=255)
                                                                            cccAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGga                                                                    >  2:2890787/1‑148 (MQ=255)
                                                                              cAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGgaga                                                                  <  2:1970462/148‑1 (MQ=255)
                                                                                ggcggcGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAAt                                                                >  1:1257784/1‑148 (MQ=255)
                                                                                ggcggcGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAAt                                                                >  1:3603036/1‑148 (MQ=255)
                                                                                ggcggcGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAAt                                                                <  1:1734094/148‑1 (MQ=255)
                                                                                 gcggcgCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAAtt                                                               >  2:2394570/1‑148 (MQ=255)
                                                                                   ggcgCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTc                                                             <  2:1918192/148‑1 (MQ=255)
                                                                                       cTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGa                                                         <  2:2273097/148‑1 (MQ=255)
                                                                                       cTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGa                                                         >  1:2881004/1‑148 (MQ=255)
                                                                                         cGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGAtt                                                       >  1:2806731/1‑148 (MQ=255)
                                                                                         cGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGAtt                                                       >  1:2806733/1‑148 (MQ=255)
                                                                                           aTCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCa                                                     >  1:588023/1‑148 (MQ=255)
                                                                                            tctcATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAg                                                    >  2:1048552/1‑148 (MQ=255)
                                                                                            tctcATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAg                                                    >  2:217247/1‑148 (MQ=255)
                                                                                            tctcATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAg                                                    >  1:728853/1‑148 (MQ=255)
                                                                                            tctcATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAAGTTCCTGATTCAg                                                    >  2:2193507/1‑148 (MQ=255)
                                                                                             ctcATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAgc                                                   >  1:23016/1‑148 (MQ=255)
                                                                                              tcATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAgcg                                                  >  2:1171620/1‑148 (MQ=255)
                                                                                              tcATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAgcg                                                  >  2:950715/1‑148 (MQ=255)
                                                                                              tcATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAgcg                                                  >  2:3802558/1‑148 (MQ=255)
                                                                                                  ttAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAGCGCGTc                                              >  2:3045592/1‑148 (MQ=255)
                                                                                                    aaCTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAGCGCGTCgg                                            >  2:2861282/1‑148 (MQ=255)
                                                                                                     aCTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAGCGCGTCGGt                                           >  2:253301/1‑148 (MQ=255)
                                                                                                         aCGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAGCGCGTCGGTAGAc                                       >  2:1167131/1‑148 (MQ=255)
                                                                                                            gtgggcgTCGGTTTGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAGCGCGTCGGTAGACAtt                                    <  1:2162140/146‑1 (MQ=255)
                                                                                                                   tCGGTTTGCTGTTGCGGCAGTTGATTCAGCAAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCCGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAGCGCGTCGGTAGACATTCCCGGCg                             >  2:2038168/1‑148 (MQ=255)
                                                                                                                      gTTTGCTGTTGCGGCAGTTGATTCAGCGAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAGCGCGTCGGTAGACATTCCCGGCGaca                          >  1:3385141/1‑148 (MQ=255)
                                                                                                                       tttGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAGCGCGTCGGTAGACATTCCCGGCGacac                         >  2:3755971/1‑148 (MQ=255)
                                                                                                                       tttGCTGTTGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAGCGCGTCGGTAGACATTCCCGGCGacac                         >  2:3511411/1‑148 (MQ=255)
                                                                                                                              ttGCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAGCGCGTCGGTAGACATTCCCGGCGACACGCTgcgc                  >  2:3480491/1‑148 (MQ=255)
                                                                                                                                gCGGCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGTTTCAGCGCGTCGGTAGACATTCCCGGCGACACGCTGCGCgg                <  2:1736234/148‑1 (MQ=255)
                                                                                                                                  ggCAGTTGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAGCGCGTCGGTAGACATTCCCGGCGACACGCTGCGCGGCt              <  1:1167131/148‑1 (MQ=255)
                                                                                                                                       ttGATTCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAGCGCGTCGGTAGACATTCCCGGCGACACGCTGCGCGGCTCGTCa         <  2:207582/148‑1 (MQ=255)
                                                                                                                                           ttCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAGCGCGTCGGTAGACATTCCCGGCGACACGCTGCGCGGCTCGTCACGCa     >  1:2023005/1‑148 (MQ=255)
                                                                                                                                           ttCAGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAGCGCGTCGGTAGACATTCCCGGCGACACGCTGCGCGGCTCGTCACGCa     >  1:2659239/1‑148 (MQ=255)
                                                                                                                                              aGCTAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAGCGCGTCGGTAGACATTCCCGGCGACACGCTGCGCGGCTCGTCACGCAtgt  <  2:2789690/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CGCCTTAAGACGTGCAGAGTCAGACTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCGAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAGAATTTCCTGATTCAGCGCGTCGGTAGACATTCCCGGCGACACGCTGCGCGGCTCGTCACGCATGT  >  CP009273/4378633‑4378922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑