Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,396,598 A→T 100% E43V (GAA→GTA)  rnr → exoribonuclease R, RNase R

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,396,5980AT98.9% 902.1 / ‑1.8 274E43V (GAA→GTA) rnrexoribonuclease R, RNase R
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base T (146/124);  total (149/125)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 274 total aligned reads displayed.

GACAACGGAGGAACCGAGATGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGAACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGG  >  CP009273/4396453‑4396742
                                                                                                                                                 |                                                                                                                                                
    aCGGAGGAACCGAGATGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGca                                                                                                                                            >  2:2547196/1‑148 (MQ=255)
    aCGGAGGAACCGAGATGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGca                                                                                                                                            >  2:1467114/1‑148 (MQ=255)
     cGGAGGAACCGAGATGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGcac                                                                                                                                           <  2:3153238/148‑1 (MQ=255)
       gaggaACCGAGATGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACAt                                                                                                                                         <  2:1733000/148‑1 (MQ=255)
        aggaACCGAGATGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACAtt                                                                                                                                        >  1:3251310/1‑148 (MQ=255)
            aCCGAGATGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAg                                                                                                                                    <  2:3637601/148‑1 (MQ=255)
             ccGAGATGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAgg                                                                                                                                   >  2:342984/1‑148 (MQ=255)
              cGAGATGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGc                                                                                                                                  >  2:3390289/1‑148 (MQ=255)
                agaTGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTGGTGAATTTATCCTCGAACATTTAACCAACCGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGa                                                                                                                                <  1:2739083/148‑1 (MQ=255)
                agaTGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGa                                                                                                                                >  1:3366872/1‑148 (MQ=255)
                  aTGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAag                                                                                                                              >  1:2916111/1‑148 (MQ=255)
                  aTGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAag                                                                                                                              >  1:3597359/1‑148 (MQ=255)
                      cacaAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGagca                                                                                                                          <  2:29663/148‑1 (MQ=255)
                      cacaAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGagca                                                                                                                          <  2:29919/148‑1 (MQ=255)
                          aGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCtt                                                                                                                      >  2:2881025/1‑148 (MQ=255)
                           gATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTg                                                                                                                     >  2:2043212/1‑148 (MQ=255)
                            aTCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGACTTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGa                                                                                                                    >  2:3181864/1‑148 (MQ=255)
                             tCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGaa                                                                                                                   >  2:1947111/1‑148 (MQ=255)
                             tCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGaa                                                                                                                   >  2:1947940/1‑148 (MQ=255)
                             tCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGaa                                                                                                                   >  1:3145150/1‑148 (MQ=255)
                               cTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAgg                                                                                                                 <  2:2837811/148‑1 (MQ=255)
                               cTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAgg                                                                                                                 <  2:3806697/148‑1 (MQ=255)
                               cTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAgg                                                                                                                 <  1:2123198/148‑1 (MQ=255)
                                tttCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGc                                                                                                                <  2:664411/148‑1 (MQ=255)
                                tttCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGc                                                                                                                <  1:38404/148‑1 (MQ=255)
                                tttCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGc                                                                                                                <  2:1744537/148‑1 (MQ=255)
                                      ggAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGt                                                                                                          >  2:2560692/1‑148 (MQ=255)
                                       gAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTc                                                                                                         >  1:2396145/1‑148 (MQ=255)
                                         aCGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTcgc                                                                                                       >  2:1179118/1‑148 (MQ=255)
                                         aCGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTcgc                                                                                                       <  2:2577302/148‑1 (MQ=255)
                                         aCGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTcgc                                                                                                       >  1:2548509/1‑148 (MQ=255)
                                          cgcgAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTcgcc                                                                                                      >  2:2162003/1‑148 (MQ=255)
                                          cgcgAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGCCCAGCCGGGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGAcgcc                                                                                                      >  2:3080305/1‑148 (MQ=255)
                                            cgAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTcgccgc                                                                                                    <  2:91734/148‑1 (MQ=255)
                                            cgAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTcgccgc                                                                                                    >  1:375910/1‑148 (MQ=255)
                                             gAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCgccgcc                                                                                                   <  1:1576106/148‑1 (MQ=255)
                                                gCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTCTCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCCGCTTGAAGGCCTGCGTCGCCGCCGgc                                                                                                >  2:3255061/1‑148 (MQ=255)
                                                gCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTgc                                                                                                >  1:3066947/1‑148 (MQ=255)
                                                 cTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTgcg                                                                                               >  1:1716374/1‑148 (MQ=255)
                                                 cTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTgcg                                                                                               >  1:1597843/1‑148 (MQ=255)
                                                 cTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTgcg                                                                                               >  2:2827799/1‑148 (MQ=255)
                                                   gAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTgcgcg                                                                                             >  2:2737332/1‑148 (MQ=255)
                                                      aaaTACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGa                                                                                          >  2:3414921/1‑148 (MQ=255)
                                                      aaaTACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGa                                                                                          <  1:2267227/148‑1 (MQ=255)
                                                      aaaTACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGa                                                                                          <  1:2819913/148‑1 (MQ=255)
                                                               aaTCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGcgcg                                                                                 <  2:1530742/148‑1 (MQ=255)
                                                               aaTCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGcgcg                                                                                 <  2:1124560/148‑1 (MQ=255)
                                                                atcccatcccTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGa                                                                                >  2:3083866/1‑148 (MQ=255)
                                                                    catcccTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGt                                                                            >  1:2705162/1‑148 (MQ=255)
                                                                    catcccTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGt                                                                            <  2:2856848/148‑1 (MQ=255)
                                                                       cccTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCTCATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCaa                                                                         <  2:876512/148‑1 (MQ=255)
                                                                       cccTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCaa                                                                         <  1:1927106/148‑1 (MQ=255)
                                                                       cccTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCaa                                                                         >  1:1928153/1‑148 (MQ=255)
                                                                         cTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACt                                                                       <  1:1467114/148‑1 (MQ=255)
                                                                           aGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTgg                                                                     >  2:3545864/1‑148 (MQ=255)
                                                                             tCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTc                                                                   <  2:1664378/148‑1 (MQ=255)
                                                                                 gAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCa                                                               >  1:1385456/1‑148 (MQ=255)
                                                                                  aaTTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCAc                                                              >  1:672628/1‑148 (MQ=255)
                                                                                   aTTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACt                                                             <  2:3475379/148‑1 (MQ=255)
                                                                                    tttATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACtc                                                            >  1:3709519/1‑148 (MQ=255)
                                                                                    tttATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACtc                                                            >  1:3310801/1‑148 (MQ=255)
                                                                                           tCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCa                                                     >  1:1765359/1‑148 (MQ=255)
                                                                                           tCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCa                                                     >  1:2629355/1‑148 (MQ=255)
                                                                                            cGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAg                                                    <  1:2333275/148‑1 (MQ=255)
                                                                                               aCATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGc                                                 >  1:1603508/1‑148 (MQ=255)
                                                                                                cATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCt                                                <  2:1281754/148‑1 (MQ=255)
                                                                                                 aTTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTa                                               <  1:1161354/148‑1 (MQ=255)
                                                                                                 aTTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTa                                               <  1:1947198/148‑1 (MQ=255)
                                                                                                 aTTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTa                                               <  1:3431550/148‑1 (MQ=255)
                                                                                                  tttAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTAt                                              >  1:906498/1‑148 (MQ=255)
                                                                                                     cacccaccGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACGGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATgcg                                           <  2:1354013/141‑1 (MQ=255)
                                                                                                     aaCCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATgcg                                           <  2:3156687/148‑1 (MQ=255)
                                                                                                     aaCCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATgcg                                           <  2:3131841/148‑1 (MQ=255)
                                                                                                      aCCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATgcgc                                          >  1:1070060/1‑148 (MQ=255)
                                                                                                      aCCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATgcgc                                          >  1:3014102/1‑148 (MQ=255)
                                                                                                      aCCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATgcgc                                          >  1:410863/1‑148 (MQ=255)
                                                                                                       ccAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCt                                         >  1:1273537/1‑148 (MQ=255)
                                                                                                        cAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTg                                        >  2:2223221/1‑148 (MQ=255)
                                                                                                               gAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAAc                                 >  2:1616644/1‑148 (MQ=255)
                                                                                                                aaaaaCCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACg                                >  1:3472749/1‑148 (MQ=255)
                                                                                                                aaaaaCCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACg                                >  1:2374156/1‑148 (MQ=255)
                                                                                                                 aaaaCCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCATCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGAGCTGCCGGAACga                               >  2:2481440/1‑147 (MQ=255)
                                                                                                                 aaaaCCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGc                               <  2:2813070/148‑1 (MQ=255)
                                                                                                                 aaaaCCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGc                               >  1:1304903/1‑148 (MQ=255)
                                                                                                                   aaCCGGCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCt                             <  2:2252382/148‑1 (MQ=255)
                                                                                                                        gCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGAcc                        <  1:2664723/148‑1 (MQ=255)
                                                                                                                        gCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGAcc                        <  1:1842646/148‑1 (MQ=255)
                                                                                                                        gCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGAcc                        <  2:2601512/148‑1 (MQ=255)
                                                                                                                        gCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGAcc                        <  2:3660164/148‑1 (MQ=255)
                                                                                                                        gCCAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGAcc                        <  2:2598314/148‑1 (MQ=255)
                                                                                                                         ccAGCCGTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCt                       <  1:1195922/148‑1 (MQ=255)
                                                                                                                         ccAGCCGTGATGAGCAGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCt                       >  2:271337/1‑148 (MQ=255)
                                                                                                                               gTGATGAGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGaa                 <  2:797733/148‑1 (MQ=255)
                                                                                                                                 gatgaGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAg               >  1:800104/1‑148 (MQ=255)
                                                                                                                                  atgaGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGACCGCCTCGACCTGGTGACAgg              >  2:3014494/1‑148 (MQ=255)
                                                                                                                                    gaGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTa            <  2:9411/148‑1 (MQ=255)
                                                                                                                                    gaGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTa            >  1:1450079/1‑148 (MQ=255)
                                                                                                                                     aGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTAc           <  2:2916111/148‑1 (MQ=255)
                                                                                                                                     aGCTGGCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTAc           <  2:1411728/148‑1 (MQ=255)
                                                                                                                                         ggCGGTAGTACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGtt       <  1:969442/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GACAACGGAGGAACCGAGATGTCACAAGATCCTTTCCAGGAACGCGAAGCTGAAAAATACGCGAATCCCATCCCTAGTCGGGAATTTATCCTCGAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGAACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTTCACTCGTCGTCAGTGCTATGCGCTGCCGGAACGCCTCGACCTGGTGAAAGGTACCGTTATTGG  >  CP009273/4396453‑4396742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑