Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,412,786 G→T 100% E42* (GAA→TAA)  ulaE → L‑xylulose 5‑phosphate 3‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,412,7860GT97.9% 584.7 / 3.4 186E42* (GAA→TAA) ulaEL‑xylulose 5‑phosphate 3‑epimerase
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base T (104/78);  total (106/80)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 186 total aligned reads displayed.

CTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACG  >  CP009273/4412642‑4412930
                                                                                                                                                |                                                                                                                                                
cTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTaaa                                                                                                                                               >  2:1495484/1‑148 (MQ=255)
cTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTaaa                                                                                                                                               >  1:1387316/1‑148 (MQ=255)
  gtgGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACt                                                                                                                                             >  1:3261745/1‑148 (MQ=255)
   tgGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTg                                                                                                                                            <  1:1464301/148‑1 (MQ=255)
   tgGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTg                                                                                                                                            >  2:3778949/1‑148 (MQ=255)
          aaGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGACCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCg                                                                                                                                     >  2:3046086/1‑148 (MQ=255)
           aGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGc                                                                                                                                    <  1:1917859/148‑1 (MQ=255)
             gAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCt                                                                                                                                  >  1:2074479/1‑148 (MQ=255)
                cGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTc                                                                                                                               >  1:3022916/1‑148 (MQ=255)
                   atatGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTcgcg                                                                                                                            >  2:852778/1‑148 (MQ=255)
                   atatGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTcgcg                                                                                                                            >  1:310787/1‑148 (MQ=255)
                   atatGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTcgcg                                                                                                                            >  1:1776702/1‑148 (MQ=255)
                     atGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGcc                                                                                                                          >  1:1000425/1‑148 (MQ=255)
                        ttgtCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCa                                                                                                                       >  2:1855710/1‑148 (MQ=255)
                         tgtCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCaa                                                                                                                      >  2:2426811/1‑148 (MQ=255)
                         tgtCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCaa                                                                                                                      <  2:1783919/148‑1 (MQ=255)
                            ccAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTg                                                                                                                   >  1:3242458/1‑148 (MQ=255)
                               aacaaaTCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAg                                                                                                                >  1:2614068/1‑148 (MQ=255)
                                    aTCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCcgcg                                                                                                           >  1:2890603/1‑148 (MQ=255)
                                    aTCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCcgcg                                                                                                           >  1:2889615/1‑148 (MQ=255)
                                    aTCCCGCATGGCATCTATGAAAAAGCGCTCCCCGCCGGCGAGTGCTGGCTGGAACGCCTGCAACAGGCACAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGTCGATCGCCTGTCGCGCCTCAACTGGAGCcgcg                                                                                                           >  1:2930562/1‑148 (MQ=255)
                                       ccGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGagc                                                                                                        >  1:3718603/1‑148 (MQ=255)
                                       ccGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGagc                                                                                                        >  1:1770749/1‑148 (MQ=255)
                                       ccGCTTGGCATCTATGAAAAAGCGCGCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGagc                                                                                                        <  1:1299938/148‑1 (MQ=255)
                                        cGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGagca                                                                                                       >  1:1014954/1‑148 (MQ=255)
                                        cGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGagca                                                                                                       <  1:1533156/148‑1 (MQ=255)
                                          cTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGagcagc                                                                                                     >  1:3576439/1‑148 (MQ=255)
                                            tGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGt                                                                                                   >  2:1839658/1‑148 (MQ=255)
                                            tGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGt                                                                                                   <  2:3210926/148‑1 (MQ=255)
                                             ggCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTc                                                                                                  <  1:2598007/148‑1 (MQ=255)
                                              gCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCt                                                                                                 >  1:494521/1‑148 (MQ=255)
                                              gCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCt                                                                                                 >  1:1364905/1‑148 (MQ=255)
                                              gCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCt                                                                                                 <  1:1265680/148‑1 (MQ=255)
                                                 tCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGgc                                                                                              >  1:2758104/1‑148 (MQ=255)
                                                  cTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGgcg                                                                                             >  1:3413632/1‑148 (MQ=255)
                                                  cTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGgcg                                                                                             <  1:689856/148‑1 (MQ=255)
                                                  cTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGgcg                                                                                             <  1:3434620/148‑1 (MQ=255)
                                                  cTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGgcg                                                                                             >  2:3179627/1‑148 (MQ=255)
                                                  cTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGgcg                                                                                             <  1:689877/148‑1 (MQ=255)
                                                  cTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGgcg                                                                                             <  1:689884/148‑1 (MQ=255)
                                                   tATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGgcgc                                                                                            <  2:257390/148‑1 (MQ=255)
                                                     tGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTg                                                                                          <  2:1821514/148‑1 (MQ=255)
                                                     tGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTg                                                                                          <  2:1714379/148‑1 (MQ=255)
                                                         aaaGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAACTGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCa                                                                                      >  2:3412605/1‑148 (MQ=255)
                                                         aaaGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCa                                                                                      >  2:600448/1‑148 (MQ=255)
                                                         aaaGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCa                                                                                      >  2:2767102/1‑148 (MQ=255)
                                                          aaGCGCTCCCCGCCGGGGAGTGCTGGCTGGCACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCaa                                                                                     <  1:3590742/148‑1 (MQ=255)
                                                          aaGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCac                                                                                     >  1:504690/1‑147 (MQ=255)
                                                           aGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAAt                                                                                    >  2:1278367/1‑148 (MQ=255)
                                                           aGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAAt                                                                                    >  1:417250/1‑148 (MQ=255)
                                                            gcgcTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATg                                                                                   <  1:2325524/148‑1 (MQ=255)
                                                            gcgcTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATg                                                                                   >  1:1067541/1‑148 (MQ=255)
                                                             cgcTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGc                                                                                  >  2:355774/1‑148 (MQ=255)
                                                               cTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGa                                                                                <  2:133728/148‑1 (MQ=255)
                                                               cTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGa                                                                                >  1:3662950/1‑148 (MQ=255)
                                                                 ccccGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGAtt                                                                              >  2:3252371/1‑148 (MQ=255)
                                                                   ccgccgGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGAttgt                                                                            <  2:3085803/148‑1 (MQ=255)
                                                                         gggAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAAc                                                                      >  1:24590/1‑148 (MQ=255)
                                                                           gAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCg                                                                    >  1:1582722/1‑148 (MQ=255)
                                                                                  ggctggAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGgcgtgcg                                                             >  1:314462/1‑148 (MQ=255)
                                                                                  ggctggAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGgcgtgcg                                                             >  1:693258/1‑148 (MQ=255)
                                                                                      ggAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGgcgtgcgcgtg                                                         <  1:2501194/148‑1 (MQ=255)
                                                                                      ggAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGgcgtgcgcgtg                                                         <  2:1387316/148‑1 (MQ=255)
                                                                                       gAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGgcgtgcgcgtgc                                                        >  1:3672989/1‑148 (MQ=255)
                                                                                          cGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGt                                                     >  2:397877/1‑148 (MQ=255)
                                                                                          cGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGt                                                     >  2:1118410/1‑148 (MQ=255)
                                                                                           gCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTc                                                    >  1:2121458/1‑148 (MQ=255)
                                                                                           gCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTc                                                    >  1:3623585/1‑148 (MQ=255)
                                                                                            ccTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTcc                                                   >  2:3217367/1‑148 (MQ=255)
                                                                                             cTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCa                                                  <  1:3722453/148‑1 (MQ=255)
                                                                                              tGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCAt                                                 >  2:2138731/1‑148 (MQ=255)
                                                                                               gCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCAtg                                                >  1:1344957/1‑148 (MQ=255)
                                                                                                      gCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCttt                                         <  1:2186404/148‑1 (MQ=255)
                                                                                                           aaCGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCt                                    <  1:1031812/148‑1 (MQ=255)
                                                                                                           aaCGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCt                                    <  1:582052/148‑1 (MQ=255)
                                                                                                            aCGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTc                                   <  2:2737200/148‑1 (MQ=255)
                                                                                                             cGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCa                                  >  1:382929/1‑148 (MQ=255)
                                                                                                             cGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCa                                  >  2:1951929/1‑148 (MQ=255)
                                                                                                               ttAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCAtc                                >  1:373067/1‑148 (MQ=255)
                                                                                                                  ggCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCAtcgtc                             >  1:3127473/1‑148 (MQ=255)
                                                                                                                  ggCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCAtcgtc                             >  2:225456/1‑148 (MQ=255)
                                                                                                                   gCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCAtcgtcg                            <  2:1211888/148‑1 (MQ=255)
                                                                                                                   gCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCAtcgtcg                            <  1:201152/148‑1 (MQ=255)
                                                                                                                   gCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCAtcgtcg                            <  1:278084/148‑1 (MQ=255)
                                                                                                                     ttCGATTTTGTCGAAATGTCGGTAGATTAAACCGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGtt                          <  2:3327941/148‑1 (MQ=255)
                                                                                                                      tCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATcgtcgtgt                         >  2:3303029/1‑146 (MQ=255)
                                                                                                                      tCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGttt                         >  1:2784904/1‑148 (MQ=255)
                                                                                                                      tCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGttt                         >  1:930712/1‑148 (MQ=255)
                                                                                                                       cGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCACCTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTc                        <  2:3371899/148‑1 (MQ=255)
                                                                                                                          ttttGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCg                     <  1:2428879/148‑1 (MQ=255)
                                                                                                                          ttttGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCg                     <  1:583563/148‑1 (MQ=255)
                                                                                                                           tttGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGc                    >  1:2509916/1‑148 (MQ=255)
                                                                                                                             tGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTg                  <  1:3778949/148‑1 (MQ=255)
                                                                                                                                  aaaTGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTGTCCCGCTGGGCAg             >  1:3126349/1‑148 (MQ=255)
                                                                                                                                    aTGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTg           >  2:1037811/1‑148 (MQ=255)
                                                                                                                                     tGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGa          <  2:1552180/148‑1 (MQ=255)
                                                                                                                                           tAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATGGTCGTTTCCCGCTGGGCAGTGAAGATGa    <  2:2890603/148‑1 (MQ=255)
                                                                                                                                           tAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATGGTCGTTTCCCGCTGGGCAGTGAAGATGa    <  2:2889615/148‑1 (MQ=255)
                                                                                                                                             gATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGAcg  <  2:310787/148‑1 (MQ=255)
                                                                                                                                             gATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGAcg  <  2:2528056/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACG  >  CP009273/4412642‑4412930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑