Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,445,719 A→T 100% T40S (ACC→TCC)  mpl → UDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,445,7190AT99.6% 829.8 / ‑4.7 249T40S (ACC→TCC) mplUDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base T (124/124);  total (125/124)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 249 total aligned reads displayed.

CGGCAGATTAAAAAAGGATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCACCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGAAAAAAATATCCCTT  >  CP009273/4445575‑4445863
                                                                                                                                                |                                                                                                                                                
cGGCAGATTAAAAAAGGATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCt                                                                                                                                               >  1:2642030/1‑148 (MQ=255)
  gCAGATTAAAAAAGGATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTa                                                                                                                                             <  2:2037680/148‑1 (MQ=255)
  gCAGATTAAAAAAGGATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTa                                                                                                                                             <  2:106451/148‑1 (MQ=255)
   cAGATTAAAAAAGGATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTAc                                                                                                                                            >  2:3102721/1‑148 (MQ=255)
    aGATTAAAAAAGGATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACt                                                                                                                                           <  1:2674459/148‑1 (MQ=255)
     gATTAAAAAAGGATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACtt                                                                                                                                          >  1:3100905/1‑148 (MQ=255)
     gATTAAAAAAGGATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACtt                                                                                                                                          >  1:179927/1‑148 (MQ=255)
      aTTAAAAAAGGATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTg                                                                                                                                         <  2:2261288/148‑1 (MQ=255)
         aaaaaaGGATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTgaga                                                                                                                                      <  2:2434920/148‑1 (MQ=255)
          aaaaaGGATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGaa                                                                                                                                     >  2:2460364/1‑148 (MQ=255)
            aaaGGATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGc                                                                                                                                   >  1:17394/1‑148 (MQ=255)
              aGGATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCaa                                                                                                                                 >  2:2913777/1‑148 (MQ=255)
                gATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAgg                                                                                                                               >  2:1290783/1‑148 (MQ=255)
                gATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAgg                                                                                                                               >  2:1246915/1‑148 (MQ=255)
                  tAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCa                                                                                                                             >  2:565807/1‑148 (MQ=255)
                  tAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCa                                                                                                                             >  1:132733/1‑148 (MQ=255)
                   aGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCAt                                                                                                                            <  1:1836820/148‑1 (MQ=255)
                     tGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTg                                                                                                                          >  2:864539/1‑148 (MQ=255)
                        cGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGc                                                                                                                       <  1:1582753/148‑1 (MQ=255)
                          tATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTg                                                                                                                     <  2:2098458/148‑1 (MQ=255)
                           aTGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGa                                                                                                                    <  2:1832522/148‑1 (MQ=255)
                           aTGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGa                                                                                                                    <  1:882463/148‑1 (MQ=255)
                           aTGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGa                                                                                                                    <  1:2895777/148‑1 (MQ=255)
                           aTGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGa                                                                                                                    >  2:1958552/1‑148 (MQ=255)
                             gcgcATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGAtt                                                                                                                  <  2:1786767/148‑1 (MQ=255)
                             gcgcATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTAATTGAGAAGCAAGGCATTGAGCTGAtt                                                                                                                  >  1:1017353/1‑148 (MQ=255)
                               gcATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCa                                                                                                                <  1:2933370/148‑1 (MQ=255)
                                 aTTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAgg                                                                                                              >  2:1504229/1‑148 (MQ=255)
                                 aTTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAgg                                                                                                              <  1:2073102/148‑1 (MQ=255)
                                 aTTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGGTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAgg                                                                                                              >  2:3188632/1‑148 (MQ=255)
                                  ttCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAggg                                                                                                             >  1:2018226/1‑148 (MQ=255)
                                     atatTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTa                                                                                                          <  2:136365/148‑1 (MQ=255)
                                       atTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACg                                                                                                        >  1:3518743/1‑148 (MQ=255)
                                        ttttAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGa                                                                                                       <  2:3004293/148‑1 (MQ=255)
                                              gAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATgccag                                                                                                 >  2:2814831/1‑148 (MQ=255)
                                              gAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATgccag                                                                                                 >  2:577006/1‑148 (MQ=255)
                                              gAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATgccag                                                                                                 >  1:2363441/1‑148 (MQ=255)
                                                  ttGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGccagccag                                                                                             <  2:2685212/148‑1 (MQ=255)
                                                  ttGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGccagccag                                                                                             >  1:1409793/1‑148 (MQ=255)
                                                         acaTTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGc                                                                                      >  1:1262688/1‑148 (MQ=255)
                                                             ttATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCa                                                                                  >  2:2184195/1‑148 (MQ=255)
                                                             ttATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCa                                                                                  >  1:962338/1‑148 (MQ=255)
                                                             ttATGGGCGGTCTGGCGATGCTGGCGCGCCAGGGAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCAGATGAGCTCCTTAATTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCACCCAGCACGAGCCGCa                                                                                  >  1:714847/1‑148 (MQ=255)
                                                              tATGGGCGGTCTGGCGATGCTGGCGCTCCAGTTAGGCCATGAAGTAACGGTTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAg                                                                                 <  2:149998/148‑1 (MQ=255)
                                                              tATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAg                                                                                 <  1:3313575/148‑1 (MQ=255)
                                                               aTGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGc                                                                                >  2:1618605/1‑148 (MQ=255)
                                                                    cGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGAt                                                                           <  2:2279888/148‑1 (MQ=255)
                                                                    cGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGAt                                                                           <  2:2280059/148‑1 (MQ=255)
                                                                        cTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTgg                                                                       <  1:1841361/148‑1 (MQ=255)
                                                                          ggCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTg                                                                     <  2:2832384/148‑1 (MQ=255)
                                                                          ggCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTg                                                                     <  2:1662520/148‑1 (MQ=255)
                                                                             gATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGatt                                                                  <  2:3769131/148‑1 (MQ=255)
                                                                             gATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGatt                                                                  <  2:389414/148‑1 (MQ=255)
                                                                              aTGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGatta                                                                 <  2:2508131/148‑1 (MQ=255)
                                                                               tGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGattat                                                                <  2:918087/148‑1 (MQ=255)
                                                                                gCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGattatt                                                               <  2:3374095/148‑1 (MQ=255)
                                                                                gCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGattatt                                                               <  1:2491184/148‑1 (MQ=255)
                                                                                gCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGattatt                                                               >  2:2660889/1‑148 (MQ=255)
                                                                                gCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGattatt                                                               >  2:2477443/1‑148 (MQ=255)
                                                                                     cgcgcCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCaa                                                          <  1:3627209/148‑1 (MQ=255)
                                                                                     cgcgcCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCaa                                                          <  1:474453/148‑1 (MQ=255)
                                                                                             ttAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGa                                                  >  1:3189751/1‑148 (MQ=255)
                                                                                             ttAGGCCATGAAGTAACGGGTTCGGACGACAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGa                                                  >  2:1025183/1‑148 (MQ=255)
                                                                                              tAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGAc                                                 >  1:2990311/1‑148 (MQ=255)
                                                                                                ggCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGAccc                                               >  2:2375539/1‑148 (MQ=255)
                                                                                                 gCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCg                                              >  1:2164278/1‑148 (MQ=255)
                                                                                                    aTGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTgg                                           >  1:2796722/1‑148 (MQ=255)
                                                                                                      gAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGaa                                         <  2:1617156/148‑1 (MQ=255)
                                                                                                      gAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGaa                                         <  1:894940/148‑1 (MQ=255)
                                                                                                         gTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATc                                      <  1:251882/148‑1 (MQ=255)
                                                                                                          tAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATcc                                     >  2:3706818/1‑148 (MQ=255)
                                                                                                          tAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATcc                                     >  2:3706840/1‑148 (MQ=255)
                                                                                                          tAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATcc                                     >  2:3418518/1‑148 (MQ=255)
                                                                                                            aCGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCgt                                   >  1:1920726/1‑148 (MQ=255)
                                                                                                            aCGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCgt                                   >  1:3175928/1‑148 (MQ=255)
                                                                                                            aCGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCgt                                   >  2:2850740/1‑148 (MQ=255)
                                                                                                             cGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCgtg                                  <  2:1918483/148‑1 (MQ=255)
                                                                                                              gggTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCgtgt                                 <  1:1112802/148‑1 (MQ=255)
                                                                                                               ggTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCgtgtg                                <  1:2591311/148‑1 (MQ=255)
                                                                                                                    ggACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGaa                           >  2:2827851/1‑148 (MQ=255)
                                                                                                                     gACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAg                          <  2:823636/148‑1 (MQ=255)
                                                                                                                      aCGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGc                         <  2:179816/148‑1 (MQ=255)
                                                                                                                       cGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCg                        <  1:565900/148‑1 (MQ=255)
                                                                                                                         ccAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGt                      >  2:2416793/1‑148 (MQ=255)
                                                                                                                           aaTGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTAc                    >  1:375178/1‑148 (MQ=255)
                                                                                                                             tgtgTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTg                  >  1:2740337/1‑148 (MQ=255)
                                                                                                                                   tCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGaaaaa            <  2:3547063/148‑1 (MQ=255)
                                                                                                                                    ccgccgATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGaaaaaa           <  1:1102224/148‑1 (MQ=255)
                                                                                                                                     cgccgATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGaaaaaaa          <  1:3812858/148‑1 (MQ=255)
                                                                                                                                      gccgATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGAAAAAAat         <  2:2018199/148‑1 (MQ=255)
                                                                                                                                      gccgATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGAAAAAAat         <  2:1740731/148‑1 (MQ=255)
                                                                                                                                       ccgATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGAAAAAAata        <  1:2246228/148‑1 (MQ=255)
                                                                                                                                        cgATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGAAAAAAatat       <  2:118941/148‑1 (MQ=255)
                                                                                                                                         gATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGAAAAAAATATc      <  1:3691245/148‑1 (MQ=255)
                                                                                                                                         gATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGAAAAAAATATc      <  2:2172892/148‑1 (MQ=255)
                                                                                                                                           tGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGAAAAAAATATccc    <  1:2269025/148‑1 (MQ=255)
                                                                                                                                           tGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGAAAAAAATATccc    >  1:634038/1‑148 (MQ=255)
                                                                                                                                           tGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGAAAAAAATATccc    <  2:1303151/148‑1 (MQ=255)
                                                                                                                                             aGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGAAAAAAATATCCCtt  <  2:3357837/148‑1 (MQ=255)
                                                                                                                                             aGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGAAAAAAATATCCCtt  >  2:756791/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CGGCAGATTAAAAAAGGATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCACCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGAAAAAAATATCCCTT  >  CP009273/4445575‑4445863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑