Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,457,499 C→A 100% R20S (CGT→AGT)  mgtA → magnesium transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,457,4990CA98.1% 667.2 / 3.2 210R20S (CGT→AGT) mgtAmagnesium transporter
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base A (96/110);  total (98/112)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 211 total aligned reads displayed.

CCCTGCTCAGCTTTATTACCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAG  >  CP009273/4457354‑4457646
                                                                                                                                                 |                                                                                                                                                   
cccTGCTCAGCTTTATTACCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGt                                                                                                                                                   <  2:3416509/148‑1 (MQ=255)
cccTGCTCAGCTTTATTACCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGt                                                                                                                                                   >  1:455932/1‑148 (MQ=255)
 ccTGCTCAGCTTTATTACCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTg                                                                                                                                                  <  1:3233169/148‑1 (MQ=255)
   tGCTCAGCTTTATTACCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGAt                                                                                                                                                <  1:3500688/148‑1 (MQ=255)
    gCTCAGCTTTATTACCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATc                                                                                                                                               >  1:2710346/1‑148 (MQ=255)
    gCTCAGCTTTATTACCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTATACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATc                                                                                                                                               >  2:483712/1‑148 (MQ=255)
     cTCAGCTTTATTACCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATcc                                                                                                                                              <  2:2099129/148‑1 (MQ=255)
     cTCAGCTTTATTACCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATcc                                                                                                                                              <  2:1829868/148‑1 (MQ=255)
     cTCAGCTTTATTACCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATcc                                                                                                                                              <  2:1580832/148‑1 (MQ=255)
         gCTTTATTACCTTCATGTAAGGCTTCGCCACGACTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTg                                                                                                                                          <  1:3217846/148‑1 (MQ=255)
          cTTTATTACCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGc                                                                                                                                         <  1:239569/148‑1 (MQ=255)
          cTTTATTACCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGc                                                                                                                                         <  1:2167715/148‑1 (MQ=255)
           tttATTACCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGcc                                                                                                                                        <  2:2299362/148‑1 (MQ=255)
            ttattaCCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCt                                                                                                                                       >  1:2217898/1‑148 (MQ=255)
            ttattaCCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCt                                                                                                                                       >  2:2527743/1‑148 (MQ=255)
                taCCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGgcg                                                                                                                                   >  1:1575063/1‑148 (MQ=255)
                  ccTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGgcgcg                                                                                                                                 >  2:369424/1‑148 (MQ=255)
                        ggTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGcagcag                                                                                                                           <  1:2862256/148‑1 (MQ=255)
                        ggTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGcagcag                                                                                                                           >  2:2568433/1‑148 (MQ=255)
                         gTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGa                                                                                                                          <  2:2710346/148‑1 (MQ=255)
                           aaGGCTTCGCCACGCCTGAAGACTTTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCTCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCTGGAGACa                                                                                                                        <  2:710525/148‑1 (MQ=255)
                             ggCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGt                                                                                                                      >  1:1191974/1‑148 (MQ=255)
                              gCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTg                                                                                                                     <  2:3777851/148‑1 (MQ=255)
                                 tCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAAt                                                                                                                  >  2:3003692/1‑148 (MQ=255)
                                  cGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATa                                                                                                                 <  2:157419/148‑1 (MQ=255)
                                  cGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATa                                                                                                                 <  1:1389196/148‑1 (MQ=255)
                                  cGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATa                                                                                                                 <  1:1778081/148‑1 (MQ=255)
                                     cACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACgg                                                                                                              <  1:2598898/148‑1 (MQ=255)
                                     cACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACgg                                                                                                              <  1:2600087/148‑1 (MQ=255)
                                     cACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACgg                                                                                                              >  1:1273254/1‑148 (MQ=255)
                                       cGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACggtg                                                                                                            >  1:1025184/1‑148 (MQ=255)
                                           tGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTcc                                                                                                        <  1:2937698/148‑1 (MQ=255)
                                           tGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTcc                                                                                                        >  2:2206234/1‑148 (MQ=255)
                                            gAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTccc                                                                                                       >  1:3664714/1‑148 (MQ=255)
                                            gAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGGTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTccc                                                                                                       >  2:2343871/1‑148 (MQ=255)
                                              aGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCccgc                                                                                                     >  2:3535672/1‑148 (MQ=255)
                                               gACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCccgcc                                                                                                    >  2:816063/1‑148 (MQ=255)
                                                aCATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCccgccg                                                                                                   >  2:109225/1‑148 (MQ=255)
                                                  aTTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTc                                                                                                 <  1:3180788/148‑1 (MQ=255)
                                                  aTTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTc                                                                                                 <  1:1820713/148‑1 (MQ=255)
                                                      cTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTa                                                                                             <  2:130478/148‑1 (MQ=255)
                                                        gTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAg                                                                                           <  1:2504422/148‑1 (MQ=255)
                                                        gTACTGGTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAg                                                                                           <  2:2542106/148‑1 (MQ=255)
                                                          aCTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTg                                                                                         >  2:1975724/1‑148 (MQ=255)
                                                          aCTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTg                                                                                         >  2:43573/1‑148 (MQ=255)
                                                            tGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTgcg                                                                                       >  1:3606770/1‑148 (MQ=255)
                                                             gTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTgcgc                                                                                      <  1:681909/148‑1 (MQ=255)
                                                               ttCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCAt                                                                                    >  1:3327516/1‑148 (MQ=255)
                                                               ttCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCAt                                                                                    <  1:14434/148‑1 (MQ=255)
                                                                 cAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTg                                                                                  >  1:793192/1‑148 (MQ=255)
                                                                 cAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTg                                                                                  <  2:1667228/148‑1 (MQ=255)
                                                                 cAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTg                                                                                  >  1:3103983/1‑148 (MQ=255)
                                                                    aCAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCt                                                                               >  1:370936/1‑148 (MQ=255)
                                                                      aGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGa                                                                             >  1:3012429/1‑148 (MQ=255)
                                                                           ggAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATg                                                                        <  2:2638136/148‑1 (MQ=255)
                                                                            gAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATgg                                                                       <  2:311594/148‑1 (MQ=255)
                                                                               ggACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGACTGAAAATGGCgg                                                                    >  2:3589481/1‑148 (MQ=255)
                                                                                 aCTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTg                                                                  >  2:3376874/1‑148 (MQ=255)
                                                                                  cTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGa                                                                 <  2:3300789/148‑1 (MQ=255)
                                                                                    ccTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATg                                                               >  1:2426946/1‑148 (MQ=255)
                                                                                    ccTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATg                                                               >  1:3603700/1‑148 (MQ=255)
                                                                                    ccTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCAGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATg                                                               >  1:995027/1‑148 (MQ=255)
                                                                                         tGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCga                                                          >  2:122827/1‑148 (MQ=255)
                                                                                          gTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCgaa                                                         >  1:2113539/1‑148 (MQ=255)
                                                                                           tttAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCgaag                                                        <  1:1868606/148‑1 (MQ=255)
                                                                                           tttAAAGAAATTTTTACCCGACTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCgaag                                                        >  1:678476/1‑148 (MQ=255)
                                                                                            ttAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCgaaga                                                       <  1:1707841/148‑1 (MQ=255)
                                                                                               aaGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCgaagaaga                                                    >  2:928911/1‑148 (MQ=255)
                                                                                                aGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCAGTGCCTGAAAATGGCGGTGATGCCCgaagaagaa                                                   <  1:3086903/148‑1 (MQ=255)
                                                                                                 gAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAAt                                                  >  2:1720787/1‑148 (MQ=255)
                                                                                                  aaaTTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAAtt                                                 >  1:1032757/1‑148 (MQ=255)
                                                                                                     tttttACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATtgtg                                              >  2:3813782/1‑148 (MQ=255)
                                                                                                      ttttACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTgg                                             <  2:3696814/148‑1 (MQ=255)
                                                                                                         tACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGaaa                                          <  1:3691711/148‑1 (MQ=255)
                                                                                                         tACCCGGCTCATTCGCCATTTACCCTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGaaa                                          <  1:1107915/148‑1 (MQ=255)
                                                                                                          aCCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGaaaa                                         <  1:1249015/148‑1 (MQ=255)
                                                                                                          aCCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGaaaa                                         >  1:3745432/1‑148 (MQ=255)
                                                                                                             cGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGt                                      >  2:138133/1‑148 (MQ=255)
                                                                                                             cGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGt                                      <  1:877990/148‑1 (MQ=255)
                                                                                                             cGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGt                                      <  1:3800154/148‑1 (MQ=255)
                                                                                                              ggCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGtt                                     <  1:2635/148‑1 (MQ=255)
                                                                                                                 tCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGa                                  >  2:2260023/1‑148 (MQ=255)
                                                                                                                     tCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACAcc                              >  1:1154711/1‑148 (MQ=255)
                                                                                                                     tCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACAcc                              <  2:1784721/148‑1 (MQ=255)
                                                                                                                     tCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACAcc                              >  2:2237187/1‑148 (MQ=255)
                                                                                                                     tCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACAcc                              >  1:1402141/1‑148 (MQ=255)
                                                                                                                          aTTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATcc                         >  2:1017326/1‑148 (MQ=255)
                                                                                                                            ttACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCgg                       >  1:3174834/1‑148 (MQ=255)
                                                                                                                             tACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGa                      <  2:3547752/148‑1 (MQ=255)
                                                                                                                             tACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGa                      <  2:3548787/148‑1 (MQ=255)
                                                                                                                                cTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGAAgg                   >  1:577537/1‑148 (MQ=255)
                                                                                                                                 ttCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGAAggg                  >  2:251250/1‑148 (MQ=255)
                                                                                                                                 ttCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGAAggg                  >  2:686835/1‑148 (MQ=255)
                                                                                                                                  tCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGAAGGGt                 >  2:938055/1‑148 (MQ=255)
                                                                                                                                  tCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGAAGGGt                 >  2:2851224/1‑148 (MQ=255)
                                                                                                                                  tCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCAGTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGAAGGGt                 <  1:3543257/148‑1 (MQ=255)
                                                                                                                                      gTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGAAGGGTTaaa             <  1:3032537/148‑1 (MQ=255)
                                                                                                                                       tCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGAGGTCCCGCCGTCCTTAAGTGCGCCGTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGAAGGGTTAAAt            <  1:13721/148‑1 (MQ=255)
                                                                                                                                           gTTCATAGTGATCCAGTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGAAGGGTTAAATCAgg        <  2:2687615/148‑1 (MQ=255)
                                                                                                                                                 cGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAg  >  2:3404164/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CCCTGCTCAGCTTTATTACCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAG  >  CP009273/4457354‑4457646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑