BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,472,936	G→T	6.5%	P240Q (CCG→CAG)	valS ←	valyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,472,936	0	G	T	6.5%	858.1 / 22.2	229	P240Q (CCG→CAG)	valS	valyl‑tRNA synthetase
Reads supporting (aligned to +/- strand): ref base G (113/101); new base T (5/10); total (118/111)
Fisher's exact test for biased strand distribution p-value = 1.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

Only 100 of 240 total aligned reads displayed.

ACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGAACCTTTCGATTCG  >  CP009273/4472789‑4473082

aCTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAAcgcg                                                                                                                                                    <  2:2348462/148‑1 (MQ=255)

aCTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAAcgcg                                                                                                                                                    <  2:2493469/148‑1 (MQ=255)

 cTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCgg                                                                                                                                                   <  1:2742691/148‑1 (MQ=255)

  ttCATAGTCGTTAAAGTCGTGCGCCGGATTGATTTTCACGCAGCCGGTGCCATTTTCCATGTCGGCGGGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTTACGCGGa                                                                                                                                                  >  1:3352031/1‑148 (MQ=255)

   tCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGAt                                                                                                                                                 <  2:1208711/148‑1 (MQ=255)

   tCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGAt                                                                                                                                                 <  1:942785/148‑1 (MQ=255)

   tCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGAt                                                                                                                                                 <  1:3794040/148‑1 (MQ=255)

    cATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATc                                                                                                                                                <  2:3739465/148‑1 (MQ=255)

     aTAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCt                                                                                                                                               >  2:1846331/1‑148 (MQ=255)

       aGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAAACAGATCTTTGTAACGCTGATCTTc                                                                                                                                             >  2:2769206/1‑148 (MQ=255)

          cGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCgg                                                                                                                                          >  1:1458982/1‑148 (MQ=255)

            ttAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCTGATCTTCCGGGt                                                                                                                                        >  2:662783/1‑148 (MQ=255)

            ttAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGt                                                                                                                                        >  1:2278258/1‑148 (MQ=255)

             tAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGtt                                                                                                                                       >  1:1016164/1‑148 (MQ=255)

              aaaGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTa                                                                                                                                      <  1:1547466/148‑1 (MQ=255)

               aaGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGTCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTaa                                                                                                                                     <  2:3189720/148‑1 (MQ=255)

                aGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAAc                                                                                                                                    >  1:662129/1‑148 (MQ=255)

                  tCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACgg                                                                                                                                  <  2:3435304/148‑1 (MQ=255)

                     tGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTa                                                                                                                               >  2:3100679/1‑148 (MQ=255)

                           ggAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAg                                                                                                                         <  1:2500899/148‑1 (MQ=255)

                            gAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGt                                                                                                                        <  1:218279/148‑1 (MQ=255)

                              gTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTAt                                                                                                                      <  2:3297844/148‑1 (MQ=255)

                              gTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTAt                                                                                                                      >  1:1139705/1‑148 (MQ=255)

                                  ttttCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGcc                                                                                                                  >  2:2315616/1‑148 (MQ=255)

                                   tttCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGccc                                                                                                                 <  2:170101/148‑1 (MQ=255)

                                   tttCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGccc                                                                                                                 >  2:3494567/1‑148 (MQ=255)

                                      cACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCcagc                                                                                                              <  1:269791/148‑1 (MQ=255)

                                        cGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGc                                                                                                                            <  1:1346721/132‑1 (MQ=255)

                                           aGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGt                                                                                                         <  1:411492/148‑1 (MQ=255)

                                              cGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCTGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTc                                                                                                      <  1:1951273/148‑1 (MQ=255)

                                              cGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTc                                                                                                      <  1:628987/148‑1 (MQ=255)

                                              cGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTc                                                                                                      >  2:1632363/1‑148 (MQ=255)

                                                 tGCCTTTTTCCATTTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTACGTGTTAACTGCTACGCCAGTATCGCCCAGCAGGGTTTCTgg                                                                                                   >  2:1554238/1‑148 (MQ=255)

                                                 tGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTgg                                                                                                   >  2:2494387/1‑148 (MQ=255)

                                                    cTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACg                                                                                                <  1:1468613/148‑1 (MQ=255)

                                                      ttttCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACggg                                                                                              >  2:1579051/1‑148 (MQ=255)

                                                      ttttCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACggg                                                                                              <  2:48598/148‑1 (MQ=255)

                                                         tCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAg                                                                                           <  2:2090496/148‑1 (MQ=255)

                                                           cATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTc                                                                                         >  1:2378686/1‑148 (MQ=255)

                                                           cATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTc                                                                                         >  1:3762582/1‑148 (MQ=255)

                                                            aTGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTcg                                                                                        >  2:1807754/1‑148 (MQ=255)

                                                                      gtTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTATCGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAg                                                                              <  1:1403254/148‑1 (MQ=255)

                                                                      gtTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAg                                                                              >  1:3784529/1‑148 (MQ=255)

                                                                        tcgtcgCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGAt                                                                            >  2:2252428/1‑148 (MQ=255)

                                                                        tcgtcgCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGAt                                                                            >  2:1795440/1‑148 (MQ=255)

                                                                         cgtcgCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATa                                                                           <  1:273537/148‑1 (MQ=255)

                                                                             gCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCt                                                                       <  2:1458982/148‑1 (MQ=255)

                                                                                aaCGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTa                                                                    <  1:3331206/148‑1 (MQ=255)

                                                                                aaCGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTa                                                                    <  1:3257988/148‑1 (MQ=255)

                                                                                aaCGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTa                                                                    <  2:3803271/148‑1 (MQ=255)

                                                                                aaCGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTa                                                                    <  2:815345/148‑1 (MQ=255)

                                                                                  cGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTAcc                                                                  >  1:888640/1‑148 (MQ=255)

                                                                                  cGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTAcc                                                                  <  2:2912360/148‑1 (MQ=255)

                                                                                  cGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTAcc                                                                  <  2:1003235/148‑1 (MQ=255)

                                                                                    aTCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGt                                                                <  1:3037539/148‑1 (MQ=255)

                                                                                    aTCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGt                                                                <  2:2157020/148‑1 (MQ=255)

                                                                                    aTCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGt                                                                <  2:65130/148‑1 (MQ=255)

                                                                                    aTCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGt                                                                <  2:1544791/148‑1 (MQ=255)

                                                                                     tCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTCCCGTc                                                               >  2:738368/1‑148 (MQ=255)

                                                                                      cGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCt                                                              >  1:232691/1‑148 (MQ=255)

                                                                                           tACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCTGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGt                                                         <  1:870404/148‑1 (MQ=255)

                                                                                           tACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCTGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGt                                                         <  1:2784806/148‑1 (MQ=255)

                                                                                           tACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGt                                                         <  1:649212/148‑1 (MQ=255)

                                                                                           tACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGt                                                         <  1:2570903/148‑1 (MQ=255)

                                                                                            acgacgGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGtt                                                        <  1:1369909/148‑1 (MQ=255)

                                                                                            acgacgGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGtt                                                        <  1:1787799/148‑1 (MQ=255)

                                                                                            acgacgGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGtt                                                        <  1:3272733/148‑1 (MQ=255)

                                                                                                   ttAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCAc                                                 >  1:1513700/1‑148 (MQ=255)

                                                                                                   ttAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCAc                                                 >  1:322265/1‑148 (MQ=255)

                                                                                                    tAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCAcc                                                >  1:1605861/1‑148 (MQ=255)

                                                                                                    tAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCAcc                                                >  1:1059640/1‑148 (MQ=255)

                                                                                                       ccAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTc                                             >  1:2792947/1‑148 (MQ=255)

                                                                                                        cAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTca                                            >  2:2805298/1‑148 (MQ=255)

                                                                                                          gCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTcagc                                          >  2:2657891/1‑148 (MQ=255)

                                                                                                             gCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTcagccag                                       <  1:3147525/148‑1 (MQ=255)

                                                                                                                 aaTGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCagcgga                                   >  2:3385533/1‑148 (MQ=255)

                                                                                                                  aTGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCagcggat                                  >  2:3515852/1‑148 (MQ=255)

                                                                                                                   tGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCagcggata                                 >  1:2861406/1‑148 (MQ=255)

                                                                                                                   tGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCagcggata                                 <  1:3515289/148‑1 (MQ=255)

                                                                                                                     aCATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCagcggatagc                               >  1:24450/1‑148 (MQ=255)

                                                                                                                        tatTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCagcggatagcgga                            >  2:130312/1‑148 (MQ=255)

                                                                                                                         atTTGCCAATCAGATCTTTGTAACGCTGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCagcggatagcggat                           >  1:2907964/1‑148 (MQ=255)

                                                                                                                         atTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCagcggatagcggat                           >  2:35978/1‑148 (MQ=255)

                                                                                                                         atTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCagcggatagcggat                           >  2:2691983/1‑148 (MQ=255)

                                                                                                                         atTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCagcggatagcggat                           >  1:486637/1‑148 (MQ=255)

                                                                                                                          tttGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGAtg                          >  2:305844/1‑148 (MQ=255)

                                                                                                                            tGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGAtgtg                        <  1:1763964/148‑1 (MQ=255)

                                                                                                                               cAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCca                     <  2:3025088/148‑1 (MQ=255)

                                                                                                                               cAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCca                     >  2:136948/1‑148 (MQ=255)

                                                                                                                                 aTCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCcaca                   >  2:2967151/1‑148 (MQ=255)

                                                                                                                                  tCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACAt                  <  2:2066477/148‑1 (MQ=255)

                                                                                                                                   cAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATc                 >  2:2557566/1‑148 (MQ=255)

                                                                                                                                    aGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCg                >  1:854652/1‑148 (MQ=255)

                                                                                                                                      aTCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGaa              >  1:2489400/1‑148 (MQ=255)

                                                                                                                                       tCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGAAc             >  1:2073894/1‑148 (MQ=255)

                                                                                                                                        cTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGAAcc            <  2:2031269/148‑1 (MQ=255)

                                                                                                                                         tttGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGAACCt           <  2:1091991/148‑1 (MQ=255)

                                                                                                                                             tAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGAACCTTTCg       <  2:2981483/148‑1 (MQ=255)

                                                                                                                                                cgcgGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGAACCTTTCGAtt    <  1:36762/148‑1 (MQ=255)

                                                                                                                                                cgcgGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGAACCTTTCGAtt    <  1:35978/148‑1 (MQ=255)

ACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGTATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGAACCTTTCGATTCG  >  CP009273/4472789‑4473082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`