Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,531,143 A→T 100% I124F (ATT→TTT)  fimB → tyrosine recombinase/inversion of on/off regulator of fimA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,531,1430AT100.0% 938.3 / ‑6.7 292I124F (ATT→TTT) fimBtyrosine recombinase/inversion of on/off regulator of fimA
Reads supporting (aligned to +/- strand):  ref base A (1/0);  major base T (148/142);  minor base G (0/1);  total (149/143)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 297 total aligned reads displayed.

TTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATATTCGTC  >  CP009273/4530998‑4531287
                                                                                                                                                 |                                                                                                                                                
ttCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGCATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCAtttt                                                                                                                                                <  2:3595478/148‑1 (MQ=255)
     caacGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTAtctc                                                                                                                                           >  2:1707275/1‑148 (MQ=255)
       acGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGa                                                                                                                                         <  2:1388690/148‑1 (MQ=255)
          cACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACtt                                                                                                                                      <  1:2900607/148‑1 (MQ=255)
              cGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCgg                                                                                                                                  >  2:3147678/1‑148 (MQ=255)
                 tATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCggtgg                                                                                                                               >  1:3731694/1‑148 (MQ=255)
                 tATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCggtgg                                                                                                                               >  1:2152502/1‑148 (MQ=255)
                 tATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAACGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCggtgg                                                                                                                               <  1:2919536/148‑1 (MQ=255)
                 aaTTTAAAAAAGAAGTTCAGGCTTTAAAAAACTGGATGACTATCCGTACATCGAACCCTCATGCTAAGAGCGAGAGCGTAATTTTATCACGTAAGTGGAAACCGCTTTCACGGAAACACTATTAACAAATTATATCGACTTCAggaag                                                                                                                               >  2:862485/2‑145 (MQ=255)
                  aTTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCggtggt                                                                                                                              <  2:1149822/148‑1 (MQ=255)
                  aTTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCggtggt                                                                                                                              >  1:2310496/1‑148 (MQ=255)
                  aTTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCggtggt                                                                                                                              >  1:1926310/1‑148 (MQ=255)
                   ttGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTa                                                                                                                             >  2:264566/1‑148 (MQ=255)
                   ttGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTa                                                                                                                             >  2:3470738/1‑148 (MQ=255)
                      aaTAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATg                                                                                                                          >  2:1595357/1‑148 (MQ=255)
                      aaTAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACAATTTTATCTAGACTTCCGGTGGTAATg                                                                                                                          >  2:2114172/1‑148 (MQ=255)
                        tAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGcc                                                                                                                        >  2:1027853/1‑148 (MQ=255)
                        tAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGcc                                                                                                                        >  1:629207/1‑148 (MQ=255)
                          aagaagTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCgg                                                                                                                      >  2:2449562/1‑148 (MQ=255)
                           agaagTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCggg                                                                                                                     >  1:2862136/1‑148 (MQ=255)
                           agaagTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCggg                                                                                                                     >  2:1649208/1‑148 (MQ=255)
                           agaagTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGGTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCggg                                                                                                                     >  1:3005194/1‑148 (MQ=255)
                               gTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGt                                                                                                                 >  2:3412399/1‑148 (MQ=255)
                                ttCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTc                                                                                                                >  1:2747757/1‑148 (MQ=255)
                                       tttAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGgag                                                                                                         <  1:2196190/148‑1 (MQ=255)
                                        ttAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGgaga                                                                                                        >  1:1865201/1‑148 (MQ=255)
                                          aaaaaaCTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGAtt                                                                                                      >  1:578218/1‑148 (MQ=255)
                                          aaaaaaCTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGAtt                                                                                                      <  1:1334666/148‑1 (MQ=255)
                                           aaaaaCTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTc                                                                                                     <  1:982726/148‑1 (MQ=255)
                                             aaaCTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCAt                                                                                                   >  2:3174351/1‑148 (MQ=255)
                                              aaCTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATc                                                                                                  >  2:177221/1‑148 (MQ=255)
                                              aaCTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATc                                                                                                  <  1:1629596/148‑1 (MQ=255)
                                               aCTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTCATGCCGGGTTGTCACTGGAGATTCATcc                                                                                                 >  2:2765865/1‑148 (MQ=255)
                                               aCTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCCCTTTATCGGCAAACGTTTTAACATTTTTTCTCGACTTACGGTGGTAATGCCGGGTTGTCACTGGAGATTCATcc                                                                                                 >  1:1349186/1‑148 (MQ=255)
                                                cTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCg                                                                                                >  2:2018431/1‑148 (MQ=255)
                                                cTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCg                                                                                                >  2:1322400/1‑148 (MQ=255)
                                                cTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCg                                                                                                >  2:1323799/1‑148 (MQ=255)
                                                  ggTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGca                                                                                              >  2:3756632/1‑148 (MQ=255)
                                                  ggTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGca                                                                                              >  2:1246122/1‑148 (MQ=255)
                                                  ggTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGca                                                                                              <  1:3509025/148‑1 (MQ=255)
                                                      gAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATg                                                                                          <  2:1050110/148‑1 (MQ=255)
                                                        gTATCCGTTCTTCGTACCCGCATGCTGAGAGCGAGTGTGTATTTTTATCACTTTTGGGTAATCCGCTTTCTCGGCTACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGtt                                                                                        <  1:1391180/148‑1 (MQ=255)
                                                         tATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTa                                                                                       >  2:185598/1‑148 (MQ=255)
                                                         tATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTa                                                                                       >  1:26506/1‑148 (MQ=255)
                                                             cGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGcc                                                                                   >  2:3704055/1‑148 (MQ=255)
                                                               tACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCAt                                                                                 >  1:2014008/1‑148 (MQ=255)
                                                                 cTTCGTACCAGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTc                                                                               >  2:2515668/1‑148 (MQ=255)
                                                                   tCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCgt                                                                             >  2:2932293/1‑148 (MQ=255)
                                                                    cGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCgtg                                                                            <  2:408504/148‑1 (MQ=255)
                                                                        cccGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGt                                                                        <  1:731732/148‑1 (MQ=255)
                                                                          cGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGttt                                                                      >  1:3119870/1‑148 (MQ=255)
                                                                            cATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTg                                                                    <  1:2377357/148‑1 (MQ=255)
                                                                            cATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTg                                                                    <  2:2868522/148‑1 (MQ=255)
                                                                            cATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTGTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTg                                                                    <  2:1478858/148‑1 (MQ=255)
                                                                             aTGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGc                                                                   >  2:2011906/1‑148 (MQ=255)
                                                                             aTGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGc                                                                   <  1:812247/148‑1 (MQ=255)
                                                                              tGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCt                                                                  >  2:2872208/1‑148 (MQ=255)
                                                                               gCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCtt                                                                 >  1:1697193/1‑148 (MQ=255)
                                                                               gCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCtt                                                                 <  2:2287281/148‑1 (MQ=255)
                                                                                      gCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGaa                                                          <  1:1653098/148‑1 (MQ=255)
                                                                                       cGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAat                                                         >  1:3306579/1‑148 (MQ=255)
                                                                                       cGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAat                                                         >  1:2668998/1‑148 (MQ=255)
                                                                                          gTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATg                                                      >  1:2575002/1‑148 (MQ=255)
                                                                                           tGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATgg                                                     >  1:114150/1‑148 (MQ=255)
                                                                                                aTTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATa                                                <  1:125014/148‑1 (MQ=255)
                                                                                                 tttttATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAg                                               <  1:1948958/148‑1 (MQ=255)
                                                                                                   tttATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGAt                                             <  2:2925147/148‑1 (MQ=255)
                                                                                                        cACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATAcgcg                                        >  1:842011/1‑148 (MQ=255)
                                                                                                          cGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGAc                                      <  1:1027853/148‑1 (MQ=255)
                                                                                                            tAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACtt                                    <  2:3215392/148‑1 (MQ=255)
                                                                                                              aGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTAt                                  <  1:2449562/148‑1 (MQ=255)
                                                                                                               ggggAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGACTATGGGAATAGATACGCGACTTATc                                 >  1:3619722/1‑148 (MQ=255)
                                                                                                               ggggAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATc                                 >  1:3619863/1‑148 (MQ=255)
                                                                                                                 ggAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCa                               <  2:1095708/148‑1 (MQ=255)
                                                                                                                  gAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAg                              >  1:1566206/1‑148 (MQ=255)
                                                                                                                   aaTCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAgg                             <  1:1595357/148‑1 (MQ=255)
                                                                                                                   aaTCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAgg                             <  2:1349186/148‑1 (MQ=255)
                                                                                                                    aTCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGa                            <  1:1322400/148‑1 (MQ=255)
                                                                                                                    aTCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGa                            <  1:464982/148‑1 (MQ=255)
                                                                                                                    aTCCGCTGTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGATTTGGCGATTATGGTAATAGATTCGCGACTTATCCAGGa                            <  1:2291093/148‑1 (MQ=255)
                                                                                                                     tCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGAt                           >  2:1182667/1‑148 (MQ=255)
                                                                                                                     tCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGAt                           >  2:3087667/1‑148 (MQ=255)
                                                                                                                      ccGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGAtt                          <  2:176062/148‑1 (MQ=255)
                                                                                                                      ccGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGAtt                          >  1:147281/1‑148 (MQ=255)
                                                                                                                       cGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTa                         <  1:2810719/148‑1 (MQ=255)
                                                                                                                        gCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTAt                        >  1:764619/1‑148 (MQ=255)
                                                                                                                         cTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATc                       <  2:2152798/148‑1 (MQ=255)
                                                                                                                         cTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATc                       <  2:586034/148‑1 (MQ=255)
                                                                                                                            tctcGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTg                    <  1:1810969/148‑1 (MQ=255)
                                                                                                                            tctcGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTg                    >  2:497072/1‑148 (MQ=255)
                                                                                                                                gtcAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTAACTGGATATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGTGAATAGATACGCGACTTATCCAGGATTATCTTGGGCa                <  2:2551187/146‑1 (MQ=255)
                                                                                                                                    aCAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGc            >  1:59531/1‑148 (MQ=255)
                                                                                                                                     cAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCa           >  2:3045377/1‑148 (MQ=255)
                                                                                                                                     cAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCa           <  2:629199/148‑1 (MQ=255)
                                                                                                                                       gTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAat         <  2:1871864/148‑1 (MQ=255)
                                                                                                                                       gTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAat         <  1:3470738/148‑1 (MQ=255)
                                                                                                                                        ttttACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAata        <  1:1283606/148‑1 (MQ=255)
                                                                                                                                        ttttACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAata        >  1:1077889/1‑148 (MQ=255)
                                                                                                                                          ttACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATAtt      >  1:343728/1‑148 (MQ=255)
                                                                                                                                             ccATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATATTCGt   <  1:1408072/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATATTCGTC  >  CP009273/4530998‑4531287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑