Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,571,233 G→A 100% S16F (TCT→TTT)  hsdS ← specificity determinant for hsdM and hsdR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,571,2330GA100.0% 672.1 / NA 201S16F (TCT→TTT) hsdSspecificity determinant for hsdM and hsdR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (110/91);  total (110/91)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 201 total aligned reads displayed.

TTTAGGAACAAAAACCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAG  >  CP009273/4571088‑4571377
                                                                                                                                                 |                                                                                                                                                
  tAGGAACAAAAACCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATAc                                                                                                                                              <  2:3183034/148‑1 (MQ=255)
  tAGGAACAAAAACCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATAc                                                                                                                                              <  1:957764/148‑1 (MQ=255)
   aGGAACAAAAACCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACt                                                                                                                                             <  1:2191064/148‑1 (MQ=255)
     gAACAAAAACCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTgg                                                                                                                                           <  2:2203865/148‑1 (MQ=255)
         aaaaaCCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCg                                                                                                                                       <  1:3705677/148‑1 (MQ=255)
            aaCCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATa                                                                                                                                    >  2:3384822/1‑148 (MQ=255)
            aaCCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATa                                                                                                                                    >  1:3565838/1‑148 (MQ=255)
               cAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAacc                                                                                                                                 <  1:1889778/148‑1 (MQ=255)
                aaGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAaccc                                                                                                                                <  1:3180178/148‑1 (MQ=255)
                  gTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAacccac                                                                                                                              >  1:3775116/1‑148 (MQ=255)
                    ccGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGATGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAacccaccc                                                                                                                            <  2:158764/148‑1 (MQ=255)
                          taTCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCgg                                                                                                                      >  2:3501436/1‑148 (MQ=255)
                          taTCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCgg                                                                                                                      >  2:2613204/1‑148 (MQ=255)
                             cAAACTTGCCATTCTGAATATTGTTCGCACGGATAATAGGCAAATAATCATCTTTTAGATAAATTATTGACTGATCTTTTTTATACTTTACTCCTCGGATTATAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCaa                                                                                                                   >  1:1349426/1‑148 (MQ=255)
                             cAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCaa                                                                                                                   >  1:3253696/1‑148 (MQ=255)
                                      cATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGc                                                                                                          >  1:2933933/1‑148 (MQ=255)
                                      cATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCCGCAATTTCCCCGc                                                                                                          >  2:228507/1‑148 (MQ=255)
                                       aTTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCa                                                                                                         >  1:2306951/1‑148 (MQ=255)
                                         tCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACt                                                                                                       >  1:449907/1‑148 (MQ=255)
                                         tCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTACTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACt                                                                                                       <  1:3575880/148‑1 (MQ=255)
                                           tGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCa                                                                                                     <  1:2607713/148‑1 (MQ=255)
                                              atatTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTc                                                                                                  <  1:741370/148‑1 (MQ=255)
                                               tatTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTcc                                                                                                 <  1:1550096/148‑1 (MQ=255)
                                               tatTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTcc                                                                                                 >  2:2976881/1‑148 (MQ=255)
                                                atTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTTCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCt                                                                                                <  2:3037707/148‑1 (MQ=255)
                                                 ttGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCtt                                                                                               >  2:2153493/1‑148 (MQ=255)
                                                  tGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTc                                                                                              <  1:63081/148‑1 (MQ=255)
                                                     tCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCAcc                                                                                           <  1:1587860/148‑1 (MQ=255)
                                                        cACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCcca                                                                                        <  1:1787602/148‑1 (MQ=255)
                                                              tAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATGCCTTCACCCCACCAAAc                                                                                  >  2:137128/1‑148 (MQ=255)
                                                               aaGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCAATCCTTCACCCCACCAAACg                                                                                 >  2:1255216/1‑148 (MQ=255)
                                                                agagGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGc                                                                                >  1:1322927/1‑148 (MQ=255)
                                                                 gagGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGct                                                                               <  2:1669817/148‑1 (MQ=255)
                                                                 gagGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGct                                                                               >  1:2365227/1‑148 (MQ=255)
                                                                  agGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTTGGGCGATAACCCACCCCTCCGGCAATGTCCCCGCACTCATTCCTTCACCCCACCAAACGctt                                                                              >  2:2590531/1‑148 (MQ=255)
                                                                  agGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGctt                                                                              <  2:360798/148‑1 (MQ=255)
                                                                  agGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGctt                                                                              >  2:1967299/1‑148 (MQ=255)
                                                                  agGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGctt                                                                              <  2:2131808/148‑1 (MQ=255)
                                                                  agGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGctt                                                                              >  2:34970/1‑148 (MQ=255)
                                                                   ggCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGcttc                                                                             <  2:817816/148‑1 (MQ=255)
                                                                     cAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGcttctt                                                                           <  1:1657416/148‑1 (MQ=255)
                                                                     cAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGcttctt                                                                           >  2:121332/1‑148 (MQ=255)
                                                                       aataatCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTcc                                                                         >  1:151415/1‑148 (MQ=255)
                                                                       aataatCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTcc                                                                         <  2:3595383/148‑1 (MQ=255)
                                                                       aataatCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTcc                                                                         >  1:1298713/1‑148 (MQ=255)
                                                                         taatCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAg                                                                       >  2:1744583/1‑148 (MQ=255)
                                                                          aatCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGc                                                                      >  2:1615763/1‑148 (MQ=255)
                                                                          aatCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGc                                                                      >  2:839286/1‑148 (MQ=255)
                                                                             catcTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAAc                                                                   <  1:3384822/148‑1 (MQ=255)
                                                                             catcTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTACCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAAc                                                                   >  1:2148871/1‑148 (MQ=255)
                                                                              atcTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACCCTTCTTACAGCAACt                                                                  >  2:2015627/1‑148 (MQ=255)
                                                                                 ttttAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCCCCCGCAAATTACCCCCACACAGTACATGACCCCCCCAAACCCATATTACAGCAACAGaa                                                               >  1:1926251/1‑147 (MQ=255)
                                                                                  tttAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACg                                                              >  1:1379738/1‑148 (MQ=255)
                                                                                  tttAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTACTTCACCCCACCAAACGCTTCTTCCAGCAACTGACg                                                              >  2:2096946/1‑148 (MQ=255)
                                                                                       aTAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCa                                                         >  2:3361894/1‑148 (MQ=255)
                                                                                       aTAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCa                                                         >  2:2961092/1‑148 (MQ=255)
                                                                                       aTAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCa                                                         >  2:1051266/1‑148 (MQ=255)
                                                                                       aTAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTACCCCCACTCAATCCTTCACCCCACCAAAAGCATCTTCCAGCAACAGACgcagca                                                         >  1:2225043/1‑148 (MQ=255)
                                                                                        tAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCaa                                                        <  1:813373/148‑1 (MQ=255)
                                                                                         aaTTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCACCTGACGCTGCaaa                                                       >  1:99218/1‑148 (MQ=255)
                                                                                         aaTTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCaaa                                                       <  2:2462217/148‑1 (MQ=255)
                                                                                         aaTTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCaaa                                                       <  2:2462323/148‑1 (MQ=255)
                                                                                          aTTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCCCCCGCAATTTACCCCCACACCATACTTAACCCCACCCAACCCATCATCCAGCAACAGACCCGTCAAAt                                                      >  1:306501/1‑148 (MQ=255)
                                                                                           tttATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATc                                                     >  1:2087171/1‑148 (MQ=255)
                                                                                           tttATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACAGACGCTGCAAATc                                                     >  1:277140/1‑148 (MQ=255)
                                                                                           tttATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCAATCCTTCACCCCACCAAACGCTTCTTCCAGCAACAGACGCTGCAAATc                                                     >  1:2206134/1‑148 (MQ=255)
                                                                                           tttATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACGCATTCCTTCACCCCACCAAACGCTTCATCCAGCAACTGACGCTGCAAATc                                                     >  1:558499/1‑148 (MQ=255)
                                                                                            ttATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCg                                                    >  2:1862170/1‑148 (MQ=255)
                                                                                            ttATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCg                                                    >  2:3752992/1‑148 (MQ=255)
                                                                                             taatGCCTGCTCTTTTTTATACGTTACACCTCGGATTAGAGTTGTGACCGTAAATACTGGTGCGATAACACACCCCTCCCGCAATTTCCCCGCACTAATTCAATCACCCCCCAAAACGCTTATTCCAGCAACTTAAGCTGCAAATCgg                                                   >  2:114774/4‑148 (MQ=255)
                                                                                               ttGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGcc                                                 >  2:1342878/1‑148 (MQ=255)
                                                                                               ttGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAAACGGcc                                                 >  2:147065/1‑148 (MQ=255)
                                                                                               ttGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCGTCCAGCAACTGACGCTGCAAATCGGcc                                                 <  2:2755700/148‑1 (MQ=255)
                                                                                                tGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCt                                                <  2:1517648/148‑1 (MQ=255)
                                                                                                  ccTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCa                                              <  2:610792/148‑1 (MQ=255)
                                                                                                  ccTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCa                                              <  2:2711385/148‑1 (MQ=255)
                                                                                                  ccTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTACCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCa                                              >  1:1448207/1‑148 (MQ=255)
                                                                                                       tctTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCCTTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCAtcgct                                         >  1:1529068/1‑148 (MQ=255)
                                                                                                       tctTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCAtcgct                                         >  1:2656487/1‑148 (MQ=255)
                                                                                                         ttttttATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCAtcgctcg                                       >  1:2034571/1‑148 (MQ=255)
                                                                                                             ttATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGcccc                                   <  2:1838868/148‑1 (MQ=255)
                                                                                                              tataCGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGccccc                                  <  1:678575/148‑1 (MQ=255)
                                                                                                                  cGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGtt                              >  1:114987/1‑148 (MQ=255)
                                                                                                                   gTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTc                             <  1:3362820/148‑1 (MQ=255)
                                                                                                                   gTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTc                             <  2:3555223/148‑1 (MQ=255)
                                                                                                                    ttACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCa                            <  1:2976881/148‑1 (MQ=255)
                                                                                                                     tACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCCGTTCAc                           >  2:445769/1‑148 (MQ=255)
                                                                                                                     tACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCACCGGCAATTTCCCCGCACGCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCACGCCCCCAGTTCAc                           >  1:1535562/1‑148 (MQ=255)
                                                                                                                       ctcctcGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGc                         >  2:3701458/1‑148 (MQ=255)
                                                                                                                        tcatcGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTACGGCAAGTTACCCGCACTCATTACATCACCCCACCAAAAGCTTCTTCCAGCAAAAGACGCTGCAAATCGGCCGCAACGCTCTCCCCCACTACAAGCa                        >  2:2829563/4‑148 (MQ=255)
                                                                                                                         cctcGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCAt                       <  1:1716914/148‑1 (MQ=255)
                                                                                                                           tcGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCa                     >  2:881925/1‑148 (MQ=255)
                                                                                                                            cGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAAAGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAg                    >  1:3517062/1‑148 (MQ=255)
                                                                                                                              gATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAgcg                  <  1:1051266/148‑1 (MQ=255)
                                                                                                                               aTTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAgcgc                 >  2:3060227/1‑148 (MQ=255)
                                                                                                                                 tAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCCCTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCAt               >  1:2501960/1‑148 (MQ=255)
                                                                                                                                     gTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAg           <  2:1058994/148‑1 (MQ=255)
                                                                                                                                     gTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAg           <  2:3283254/148‑1 (MQ=255)
                                                                                                                                            ccGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGAc    <  2:368069/148‑1 (MQ=255)
                                                                                                                                              gTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAAACGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAg  >  1:1561566/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TTTAGGAACAAAAACCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAG  >  CP009273/4571088‑4571377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑