Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,584,255 A→C 100% L95L (CTT→CTG)  yjjL ← L‑galactonate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,584,2550AC99.3% 1058.4 / ‑4.2 263L95L (CTT→CTG) yjjLL‑galactonate transporter
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base C (138/123);  total (139/124)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 263 total aligned reads displayed.

CACCAATCCCCATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGAAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTGATTACCGCCGCAAAAAAT  >  CP009273/4584110‑4584399
                                                                                                                                                 |                                                                                                                                                
   cAATCCCCATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTg                                                                                                                                             >  1:1881620/1‑148 (MQ=255)
    aaTCCCCATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTgc                                                                                                                                            >  1:1188500/1‑148 (MQ=255)
        cccATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACCTTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCaa                                                                                                                                        <  2:632088/148‑1 (MQ=255)
        cccATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCaa                                                                                                                                        <  2:2982689/148‑1 (MQ=255)
          cATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATc                                                                                                                                      <  1:761680/148‑1 (MQ=255)
                   aCGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCg                                                                                                                             <  2:1138556/148‑1 (MQ=255)
                    cGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGa                                                                                                                            >  1:1245776/1‑148 (MQ=255)
                        ccAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGa                                                                                                                        >  2:242666/1‑148 (MQ=255)
                        ccAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGa                                                                                                                        >  2:243752/1‑148 (MQ=255)
                         cAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGaa                                                                                                                       >  2:516888/1‑148 (MQ=255)
                          aaCACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGaaa                                                                                                                      <  2:3540443/148‑1 (MQ=255)
                           acacGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGaaaa                                                                                                                     >  1:530657/1‑148 (MQ=255)
                            cacGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAac                                                                                                                    <  1:1079886/148‑1 (MQ=255)
                            cacGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAac                                                                                                                    <  1:3766233/148‑1 (MQ=255)
                             acGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAaca                                                                                                                   <  2:684333/148‑1 (MQ=255)
                                    gCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGagag                                                                                                            >  1:3635281/1‑148 (MQ=255)
                                    gCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGagag                                                                                                            <  1:2393003/148‑1 (MQ=255)
                                       tAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCaa                                                                                                         >  1:2886042/1‑148 (MQ=255)
                                        aaaGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCaaa                                                                                                        >  2:3775192/1‑148 (MQ=255)
                                           gTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAgcg                                                                                                     <  1:3508623/148‑1 (MQ=255)
                                            ttGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAgcgc                                                                                                    >  1:1632215/1‑148 (MQ=255)
                                               tgCACCATGCCAGACATTGCCTGTAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCATTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGa                                                                                                 <  1:2342080/148‑1 (MQ=255)
                                               tgCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGa                                                                                                 <  1:545062/148‑1 (MQ=255)
                                                   ccATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTc                                                                                             >  1:2439804/1‑148 (MQ=255)
                                                      tGCCAGACAGTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGt                                                                                          <  1:3648915/148‑1 (MQ=255)
                                                        ccAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTgg                                                                                        <  1:47596/148‑1 (MQ=255)
                                                          aGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCa                                                                                      <  2:1245776/148‑1 (MQ=255)
                                                           gACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCAc                                                                                     <  2:3280258/148‑1 (MQ=255)
                                                            aCATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACt                                                                                    >  1:1772847/1‑148 (MQ=255)
                                                              aTTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTa                                                                                  >  2:1639182/1‑148 (MQ=255)
                                                               ttGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTaa                                                                                 >  2:2958850/1‑148 (MQ=255)
                                                                tGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAAt                                                                                >  2:1324224/1‑148 (MQ=255)
                                                                tGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAAt                                                                                >  2:2619430/1‑148 (MQ=255)
                                                                 gCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGAATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATc                                                                               <  1:1280745/148‑1 (MQ=255)
                                                                  ccTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATcc                                                                              <  2:725166/148‑1 (MQ=255)
                                                                  ccTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATcc                                                                              <  2:725343/148‑1 (MQ=255)
                                                                    tGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCa                                                                            <  2:1535423/148‑1 (MQ=255)
                                                                    tGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCa                                                                            <  2:839734/148‑1 (MQ=255)
                                                                       aaCAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAAtt                                                                         <  2:213662/148‑1 (MQ=255)
                                                                       aaCAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAAtt                                                                         >  1:2920226/1‑148 (MQ=255)
                                                                        aCAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAAttc                                                                        >  1:3328597/1‑148 (MQ=255)
                                                                        aCAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAAttc                                                                        <  1:939189/148‑1 (MQ=255)
                                                                            tGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCa                                                                    <  2:1644828/148‑1 (MQ=255)
                                                                            tGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCa                                                                    <  2:337670/148‑1 (MQ=255)
                                                                            tGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCa                                                                    >  2:331055/1‑148 (MQ=255)
                                                                              aCCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACg                                                                  >  1:1621490/1‑148 (MQ=255)
                                                                                cAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGaa                                                                <  1:2862636/148‑1 (MQ=255)
                                                                                cAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGaa                                                                <  1:1387138/148‑1 (MQ=255)
                                                                                       aCATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTaa                                                         >  2:2038728/1‑148 (MQ=255)
                                                                                        cATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTaaa                                                        >  1:291375/1‑148 (MQ=255)
                                                                                        cATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTaaa                                                        >  2:3655964/1‑148 (MQ=255)
                                                                                        cATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTaaa                                                        <  2:1004109/148‑1 (MQ=255)
                                                                                         atacccAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAAt                                                       >  2:3324826/4‑148 (MQ=255)
                                                                                          tCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAAtt                                                      >  1:2197329/1‑148 (MQ=255)
                                                                                           ccccAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCACTAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAAttt                                                     >  2:2003631/1‑148 (MQ=255)
                                                                                            cccAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTg                                                    >  2:3195398/1‑148 (MQ=255)
                                                                                            cccAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTg                                                    <  1:2333350/148‑1 (MQ=255)
                                                                                             ccAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGc                                                   >  2:125836/1‑148 (MQ=255)
                                                                                             ccAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGc                                                   >  2:1941805/1‑148 (MQ=255)
                                                                                             ccAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGc                                                   >  2:2701008/1‑148 (MQ=255)
                                                                                              cAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCt                                                  <  2:580163/148‑1 (MQ=255)
                                                                                              cAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCt                                                  >  2:2454229/1‑148 (MQ=255)
                                                                                              cAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCt                                                  <  1:2314207/148‑1 (MQ=255)
                                                                                               aGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGAAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTa                                                 <  1:2664839/148‑1 (MQ=255)
                                                                                                 tCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTAcc                                               >  1:3378044/1‑148 (MQ=255)
                                                                                                  cccAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCg                                              >  2:1380109/1‑148 (MQ=255)
                                                                                                  cccAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCg                                              >  2:2926862/1‑148 (MQ=255)
                                                                                                   ccAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGa                                             >  1:197396/1‑148 (MQ=255)
                                                                                                   ccAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGa                                             >  1:2124022/1‑148 (MQ=255)
                                                                                                     aGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACa                                           >  1:1490327/1‑148 (MQ=255)
                                                                                                     aGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACa                                           >  1:2010708/1‑148 (MQ=255)
                                                                                                      gCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAg                                          <  2:1309421/148‑1 (MQ=255)
                                                                                                      gCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAg                                          <  2:2414485/148‑1 (MQ=255)
                                                                                                       cATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGc                                         >  1:845542/1‑148 (MQ=255)
                                                                                                          cAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGaa                                      >  1:823463/1‑148 (MQ=255)
                                                                                                          cAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGaa                                      <  2:619412/148‑1 (MQ=255)
                                                                                                            ggCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACt                                    >  2:1779198/1‑148 (MQ=255)
                                                                                                             gCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTg                                   <  1:87963/148‑1 (MQ=255)
                                                                                                              cGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGc                                  <  1:2520567/148‑1 (MQ=255)
                                                                                                                  ggCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTc                              >  1:3445710/1‑148 (MQ=255)
                                                                                                                      tttACGATCCAATTGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCgaga                          <  1:394925/148‑1 (MQ=255)
                                                                                                                      tttACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCgaga                          <  1:2757727/148‑1 (MQ=255)
                                                                                                                           gATCCAATAGTGGGCCGCAAGGCAGTTGCGCAAGCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAAtt                     <  1:1303541/148‑1 (MQ=255)
                                                                                                                            aTCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAAttt                    >  1:2473232/1‑147 (MQ=255)
                                                                                                                            aTCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCAATTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAAttt                    <  1:3745624/148‑2 (MQ=255)
                                                                                                                             tCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAAttta                   <  1:1028545/148‑3 (MQ=255)
                                                                                                                             tCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAAttta                   <  1:1122010/148‑3 (MQ=255)
                                                                                                                             tCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAAttta                   <  2:3035858/148‑3 (MQ=255)
                                                                                                                             tCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAAttta                   <  2:1129722/148‑3 (MQ=255)
                                                                                                                              ccAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTAt                  <  2:372812/148‑1 (MQ=255)
                                                                                                                               cAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTAtt                 >  1:2687264/1‑148 (MQ=255)
                                                                                                                                aaTAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATttatta                <  1:1469768/148‑1 (MQ=255)
                                                                                                                                 aTAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTAc               >  2:461917/1‑148 (MQ=255)
                                                                                                                                       ggCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACcgccgc         >  2:232998/1‑148 (MQ=255)
                                                                                                                                           gCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCaaaa     >  1:1422170/1‑148 (MQ=255)
                                                                                                                                             aaGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCaaaaaa   <  2:3635349/148‑1 (MQ=255)
                                                                                                                                             aaGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCaaaaaa   <  1:3268096/148‑1 (MQ=255)
                                                                                                                                              aGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAaat  <  1:1810305/148‑1 (MQ=255)
                                                                                                                                              aGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAaat  <  2:2472238/148‑1 (MQ=255)
                                                                                                                                              aGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAaat  <  1:3700127/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CACCAATCCCCATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGAAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTGATTACCGCCGCAAAAAAT  >  CP009273/4584110‑4584399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑