Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 421,799 C→A 100% I69I (ATC→ATA)  tgt → tRNA‑guanine transglycosylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273421,7990CA99.5% 652.9 / ‑4.4 197I69I (ATC→ATA) tgttRNA‑guanine transglycosylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (98/98);  minor base G (1/0);  total (99/98)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 197 total aligned reads displayed.

GGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACG  >  CP009273/421656‑421941
                                                                                                                                               |                                                                                                                                              
ggCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGa                                                                                                                                            <  2:1333173/148‑1 (MQ=255)
 gCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGaa                                                                                                                                           >  2:642855/1‑148 (MQ=255)
      gTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGc                                                                                                                                      <  1:1156218/148‑1 (MQ=255)
       tGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCa                                                                                                                                     >  2:2693308/1‑148 (MQ=255)
        ggAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCAc                                                                                                                                    <  1:417657/148‑1 (MQ=255)
          aaaCGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACgg                                                                                                                                  >  2:121823/1‑148 (MQ=255)
          aaaCGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCACAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACgg                                                                                                                                  >  2:171172/1‑148 (MQ=255)
            aCGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCg                                                                                                                                <  1:2573089/148‑1 (MQ=255)
            aCGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCg                                                                                                                                >  2:1472400/1‑148 (MQ=255)
              gCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGAt                                                                                                                              <  2:712835/148‑1 (MQ=255)
              gCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGAt                                                                                                                              <  2:3165396/148‑1 (MQ=255)
               ccTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATc                                                                                                                             <  1:440367/148‑1 (MQ=255)
               ccTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATc                                                                                                                             >  2:1398248/1‑148 (MQ=255)
                cTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCt                                                                                                                            >  2:732951/1‑148 (MQ=255)
                cTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCt                                                                                                                            >  1:2670927/1‑148 (MQ=255)
                  tGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGc                                                                                                                          >  2:2001071/1‑148 (MQ=255)
                  tGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGc                                                                                                                          >  2:426096/1‑148 (MQ=255)
                   gTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCa                                                                                                                         <  1:2013136/148‑1 (MQ=255)
                     tttATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACg                                                                                                                       >  2:966202/1‑148 (MQ=255)
                      ttATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGa                                                                                                                      <  1:132581/148‑1 (MQ=255)
                        aTGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGAtt                                                                                                                    >  1:2610429/1‑148 (MQ=255)
                          gCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGAtttt                                                                                                                  <  1:740533/148‑1 (MQ=255)
                          gCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGAtttt                                                                                                                  <  2:390859/148‑1 (MQ=255)
                          gCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGAtttt                                                                                                                  <  1:2956167/148‑1 (MQ=255)
                                  gCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTg                                                                                                          >  2:2995152/1‑148 (MQ=255)
                                  gCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTg                                                                                                          >  1:159931/1‑148 (MQ=255)
                                     ccTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGACGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGGGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGaa                                                                                                       <  2:533437/148‑1 (MQ=255)
                                     ccTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGaa                                                                                                       <  2:765290/148‑1 (MQ=255)
                                     ccTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGaa                                                                                                       >  2:2722448/1‑148 (MQ=255)
                                       tACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAgg                                                                                                     <  1:3260618/148‑1 (MQ=255)
                                        aCGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAggg                                                                                                    >  2:2941325/1‑148 (MQ=255)
                                         cGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAgggg                                                                                                   <  1:3615011/148‑1 (MQ=255)
                                          ggCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGc                                                                                                  <  2:3036582/148‑1 (MQ=255)
                                           gCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGcc                                                                                                 >  2:2823596/1‑148 (MQ=255)
                                           gCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGcc                                                                                                 >  2:2793923/1‑148 (MQ=255)
                                           gCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGcc                                                                                                 >  1:2948178/1‑148 (MQ=255)
                                                 tAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCt                                                                                           >  1:2264773/1‑148 (MQ=255)
                                                  aaaaGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTc                                                                                          <  1:3706815/148‑1 (MQ=255)
                                                   aaaGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCa                                                                                         >  1:1052646/1‑148 (MQ=255)
                                                    aaGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCAc                                                                                        >  1:519066/1‑148 (MQ=255)
                                                             cGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCcgg                                                                               >  2:2333185/1‑148 (MQ=255)
                                                             cGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCcgg                                                                               >  2:2333146/1‑148 (MQ=255)
                                                                  gaagaaGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCt                                                                          <  2:2397381/148‑1 (MQ=255)
                                                                  gaagaaGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCt                                                                          >  1:2030713/1‑148 (MQ=255)
                                                                  gaagaaGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCt                                                                          <  1:1248781/148‑1 (MQ=255)
                                                                   aagaagTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCtt                                                                         >  1:972526/1‑148 (MQ=255)
                                                                   aagaagTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCtt                                                                         <  1:2294963/148‑1 (MQ=255)
                                                                     gaagTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTcc                                                                       >  2:3398471/1‑148 (MQ=255)
                                                                          tGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTc                                                                  >  2:561758/1‑148 (MQ=255)
                                                                           gAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCt                                                                 <  2:2651941/148‑1 (MQ=255)
                                                                            aaGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGACACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCtt                                                                <  1:136796/148‑1 (MQ=255)
                                                                            aaGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCtt                                                                <  1:642855/148‑1 (MQ=255)
                                                                             aGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTc                                                               <  2:1786165/148‑1 (MQ=255)
                                                                              gCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCa                                                              >  2:87958/1‑148 (MQ=255)
                                                                                cACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGc                                                            <  1:3391244/148‑1 (MQ=255)
                                                                                 aCTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGcc                                                           <  2:1788934/148‑1 (MQ=255)
                                                                                 aCTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGcc                                                           >  2:3331082/1‑148 (MQ=255)
                                                                                 aCTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGcc                                                           <  1:1024047/148‑1 (MQ=255)
                                                                                 aCTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACGGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGcc                                                           <  1:1024000/148‑1 (MQ=255)
                                                                                  cTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCt                                                          <  2:2221033/148‑1 (MQ=255)
                                                                                    ggCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTg                                                        <  1:3069122/148‑1 (MQ=255)
                                                                                       gcgcAACTTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCg                                                     <  2:2479764/148‑1 (MQ=255)
                                                                                        cgcAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGa                                                    <  2:3605536/148‑1 (MQ=255)
                                                                                         gcAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGat                                                   <  1:454659/148‑1 (MQ=255)
                                                                                          cAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGAGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGata                                                  >  2:3300523/1‑148 (MQ=255)
                                                                                           aaaTTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGatat                                                 <  2:1594338/148‑1 (MQ=255)
                                                                                             aTTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTc                                               >  2:1306998/1‑148 (MQ=255)
                                                                                             aTTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTc                                               <  2:357838/148‑1 (MQ=255)
                                                                                             aTTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTc                                               >  1:3061076/1‑148 (MQ=255)
                                                                                              ttATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCg                                              <  1:693689/148‑1 (MQ=255)
                                                                                               tATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGt                                             >  1:3785257/1‑148 (MQ=255)
                                                                                               tATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGt                                             <  1:1398248/148‑1 (MQ=255)
                                                                                                  ccTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTaaa                                          <  2:2915971/148‑1 (MQ=255)
                                                                                                  ccTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTaaa                                          >  1:355801/1‑148 (MQ=255)
                                                                                                  ccTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTaaa                                          <  2:44109/148‑1 (MQ=255)
                                                                                                    tCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAAt                                        <  1:73218/148‑1 (MQ=255)
                                                                                                    tCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAAt                                        <  2:1156565/148‑1 (MQ=255)
                                                                                                      ggCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCa                                      <  1:3451985/148‑1 (MQ=255)
                                                                                                        cAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCAcc                                    >  1:1353885/1‑148 (MQ=255)
                                                                                                        cAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCAGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCAcc                                    >  1:3803563/1‑148 (MQ=255)
                                                                                                           cacCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGaa                                 >  1:147730/1‑148 (MQ=255)
                                                                                                            acCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAAc                                >  1:1848077/1‑148 (MQ=255)
                                                                                                            acCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAAc                                <  2:2468230/148‑1 (MQ=255)
                                                                                                            acCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAAc                                <  1:2896685/148‑1 (MQ=255)
                                                                                                              cTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAg                              >  2:1656410/1‑148 (MQ=255)
                                                                                                               ttCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAgg                             >  1:565229/1‑148 (MQ=255)
                                                                                                               ttCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAgg                             <  1:2042382/148‑1 (MQ=255)
                                                                                                               ttCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAgg                             >  1:2780923/1‑148 (MQ=255)
                                                                                                                tCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAggg                            <  1:280871/148‑1 (MQ=255)
                                                                                                                     cTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGc                       <  2:159931/148‑1 (MQ=255)
                                                                                                                      tgtgGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCa                      <  2:2154883/148‑1 (MQ=255)
                                                                                                                      tgtgGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCa                      >  2:3240466/1‑148 (MQ=255)
                                                                                                                             gcgcCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGt               >  1:90355/1‑148 (MQ=255)
                                                                                                                             gcgcCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGt               >  2:1079083/1‑148 (MQ=255)
                                                                                                                                    ggCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGa        <  1:770739/148‑1 (MQ=255)
                                                                                                                                     gCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGAt       >  1:3270144/1‑148 (MQ=255)
                                                                                                                                       cAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCa     <  1:2477640/148‑1 (MQ=255)
                                                                                                                                       cAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCa     <  2:3543521/148‑1 (MQ=255)
                                                                                                                                        aGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCaa    <  2:2610429/148‑1 (MQ=255)
                                                                                                                                        aGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCaa    <  1:1689499/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                              
GGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACG  >  CP009273/421656‑421941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑