Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 426,157 G→T 100% E33* (GAG→TAG)  yajD → HNH nuclease family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273426,1570GT96.2% 598.1 / 3.0 235E33* (GAG→TAG) yajDHNH nuclease family protein
Reads supporting (aligned to +/- strand):  ref base G (14/1);  new base T (102/116);  total (118/117)
Fisher's exact test for biased strand distribution p-value = 3.41e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 235 total aligned reads displayed.

CATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGAT  >  CP009273/426012‑426300
                                                                                                                                                 |                                                                                                                                               
cATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAg                                                                                                                                               <  2:590806/148‑1 (MQ=255)
cATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAg                                                                                                                                               >  1:2121434/1‑148 (MQ=255)
cATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCACGCTAg                                                                                                                                               <  1:2439816/148‑1 (MQ=255)
    ttGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAgtttg                                                                                                                                           <  2:679826/148‑1 (MQ=255)
     tGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAgtttgt                                                                                                                                          <  1:1084947/148‑1 (MQ=255)
      gggAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAgtttgtt                                                                                                                                         <  2:1366410/148‑1 (MQ=255)
       ggAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTCgtttgttt                                                                                                                                        >  2:2315788/1‑148 (MQ=255)
       ggAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAgtttgttt                                                                                                                                        <  1:81306/148‑1 (MQ=255)
        gAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTa                                                                                                                                       <  1:159373/148‑1 (MQ=255)
        gAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTa                                                                                                                                       <  1:1631857/148‑1 (MQ=255)
           aCTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTc                                                                                                                                    >  2:932262/1‑148 (MQ=255)
           aCTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTc                                                                                                                                    >  1:227138/1‑148 (MQ=255)
                      cccTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTTTTACAACCTTCGTg                                                                                                                         >  1:43556/1‑148 (MQ=255)
                      cccTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTg                                                                                                                         >  1:3064311/1‑148 (MQ=255)
                      cccTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTACAACCTGCGTg                                                                                                                         >  2:2910183/1‑148 (MQ=255)
                          aCATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACt                                                                                                                     <  2:396257/148‑1 (MQ=255)
                             tttCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTAc                                                                                                                  >  2:72888/1‑148 (MQ=255)
                             tttCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTAc                                                                                                                  >  2:78523/1‑148 (MQ=255)
                              ttCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTGCAACCTGCGTGAACTTAcc                                                                                                                 >  1:3567352/1‑148 (MQ=255)
                              ttCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTAcc                                                                                                                 >  2:3365271/1‑148 (MQ=255)
                              ttCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCAGCGTGAACTTAcc                                                                                                                 >  1:1023641/1‑148 (MQ=255)
                                cATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGt                                                                                                               >  2:843953/1‑148 (MQ=255)
                                 aTCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGtt                                                                                                              <  1:1897376/148‑1 (MQ=255)
                                   ccAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTca                                                                                                            <  2:1878537/148‑1 (MQ=255)
                                      ggTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTcacca                                                                                                         >  1:1271212/1‑148 (MQ=255)
                                       gTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTcaccac                                                                                                        >  2:2150114/1‑148 (MQ=255)
                                        tAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACcaca                                                                                                       <  2:3791363/148‑1 (MQ=255)
                                         aaCTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACAt                                                                                                      <  2:110409/148‑1 (MQ=255)
                                           cTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTg                                                                                                    >  2:445337/1‑148 (MQ=255)
                                           cTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTg                                                                                                    <  1:636723/148‑1 (MQ=255)
                                           cTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTg                                                                                                    <  2:2757723/148‑1 (MQ=255)
                                           cTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTg                                                                                                    <  1:3461643/148‑1 (MQ=255)
                                             tttCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGAt                                                                                                  <  1:2361321/148‑1 (MQ=255)
                                             tttCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGAt                                                                                                  >  2:1063389/1‑148 (MQ=255)
                                                  tGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGTTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGa                                                                                             <  1:3321595/148‑1 (MQ=255)
                                                   ggCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACAATGATCACGAc                                                                                            >  1:1462481/1‑148 (MQ=255)
                                                    gCTATCATCCCAAAAAACTCTGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGAcc                                                                                           <  2:161153/148‑1 (MQ=255)
                                                    gCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGAcc                                                                                           >  2:366480/1‑148 (MQ=255)
                                                    gCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGAcc                                                                                           <  2:2556654/148‑1 (MQ=255)
                                                      tATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCAt                                                                                         <  2:1256289/148‑1 (MQ=255)
                                                       atcatcCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATa                                                                                        <  1:3693730/148‑1 (MQ=255)
                                                       atcatcCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATa                                                                                        >  1:572502/1‑148 (MQ=255)
                                                        tcatcCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATAc                                                                                       >  2:3570026/1‑148 (MQ=255)
                                                         catcCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATAcc                                                                                      >  1:1238162/1‑148 (MQ=255)
                                                         catcCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTACAACCTGCGTGAACTTACCGTTCACCACAATGATAACGACCATAcc                                                                                      >  1:1063007/1‑148 (MQ=255)
                                                         catcCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAAACTGCGTGAACTTACCGTTCACCACAATGTTAACCACCCTaac                                                                                      >  1:2566156/1‑146 (MQ=255)
                                                           tcCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCaa                                                                                    <  1:2743774/148‑1 (MQ=255)
                                                              cAAAAATCTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGTACTTACCGTTCACCACATTGATCACGACCATACCAATaa                                                                                 <  2:50962/148‑1 (MQ=255)
                                                                    aCTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCGGTTCCCGCGAGGTTGTTTATTCCAACCAGCGTGCACGTACCGTTCACCACAATGATCACCACCCTACCCAAAACCCCGa                                                                           >  1:1666699/1‑148 (MQ=255)
                                                                     cTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGaa                                                                          >  1:1223920/1‑148 (MQ=255)
                                                                     cTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCGGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGaa                                                                          >  2:2647197/1‑148 (MQ=255)
                                                                        tGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGAt                                                                       <  2:2080174/148‑1 (MQ=255)
                                                                        tGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGAt                                                                       <  2:3236691/148‑1 (MQ=255)
                                                                         gcgcGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATg                                                                      <  2:574033/148‑1 (MQ=255)
                                                                           gcgGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGc                                                                    <  1:3564522/148‑1 (MQ=255)
                                                                                tAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTaa                                                               >  2:661837/1‑148 (MQ=255)
                                                                                tAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTaa                                                               >  2:3727502/1‑148 (MQ=255)
                                                                                tAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTaa                                                               <  1:2603222/148‑1 (MQ=255)
                                                                                tAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTaa                                                               >  2:1238773/1‑148 (MQ=255)
                                                                                 aGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAAc                                                              <  2:3812062/148‑1 (MQ=255)
                                                                                   aaaGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACAATGATAACGACCATACCAATAACCCGGAAGATGGCAGTAACTg                                                            >  1:1442132/1‑148 (MQ=255)
                                                                                   aaaGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTACAACCTGCGTGAACTTAACGTTCACCACATTGATCACCACCATACCAATAACCCGGAAGATGGCAGTAACTg                                                            >  1:1980593/1‑148 (MQ=255)
                                                                                        ggCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAAt                                                       <  1:1942919/148‑1 (MQ=255)
                                                                                         gCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAAtt                                                      <  1:187672/148‑1 (MQ=255)
                                                                                             tCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGACCTTACCGTTCACCACAATGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAAttgttg                                                  >  2:2252718/1‑148 (MQ=255)
                                                                                              cgcgAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTGCCGCAAGTTTTTTTATTCCAACCAGCGTGAACATACCGTTCACCACAATGATAAAGACCCAAACAATAAACCCGAAGATTGCAGTAACTGGGAAttgtttt                                                 >  1:357726/1‑146 (MQ=255)
                                                                                                  aaaaaGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTACAACCTGCGTGAACTTAACGTTCACCACAATTATCACGACCAAACCAAAAACCCCGAAGATGGCAGTAACTGGGAATTTTTgtgtat                                             >  1:758257/1‑146 (MQ=255)
                                                                                                    aaaGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGACGATGGCAGTAACTGGGAATTGTTGTGTctct                                           >  1:2161447/1‑148 (MQ=255)
                                                                                                    aaaGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTctct                                           <  1:528909/148‑1 (MQ=255)
                                                                                                    aaaGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTACAACCAGCGTGAACTTACCGTTCACCACATTTATCACGACCAAAACAAAAAACCGGAAGATGGCAGTAACTGGGAATTGTTGTGTctct                                           >  1:1483036/1‑148 (MQ=255)
                                                                                                     aaGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTa                                          >  2:1824811/1‑148 (MQ=255)
                                                                                                      aGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTAt                                         <  1:261900/148‑1 (MQ=255)
                                                                                                       gCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTAtt                                        >  2:1774250/1‑148 (MQ=255)
                                                                                                        cATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCCGTAACTGGGACTTGTTGTGTCTCTATTg                                       >  2:1691401/1‑148 (MQ=255)
                                                                                                        cATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTg                                       >  2:135622/1‑148 (MQ=255)
                                                                                                        cATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTg                                       <  1:3539616/148‑1 (MQ=255)
                                                                                                         aTTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTACAACCTGCGTGAACTTACCGTTCACCACATTTATCACGACCATACCAAAAACCCCGAAGATGGCAGTAACTGGGAATTGTTTTGTGTCTATTga                                      >  1:161631/1‑147 (MQ=255)
                                                                                                          ttAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGcc                                     <  2:839886/148‑1 (MQ=255)
                                                                                                           tAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTGCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCa                                    >  1:1494289/1‑148 (MQ=255)
                                                                                                                 tCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCa                              >  2:300566/1‑148 (MQ=255)
                                                                                                                  cTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCAt                             >  2:3462475/1‑148 (MQ=255)
                                                                                                                     tCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAAATTAACGTTAACCACAATGATAACGACCATACCAAAAACCCGGAAGATGGCAGTAACTGGGAATTTTTGTGTCTCTATTGCCACGATCATGAg                          >  1:3374800/1‑148 (MQ=255)
                                                                                                                      ccGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGc                         <  2:179879/148‑1 (MQ=255)
                                                                                                                      ccGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGc                         <  1:2808076/148‑1 (MQ=255)
                                                                                                                      ccGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACATACCGGTCACCACAATGTTCACGACCAAACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTGTATTTCCACGATCATGAGc                         >  1:2883406/1‑148 (MQ=255)
                                                                                                                        gTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATCACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCAt                       <  2:2350489/148‑1 (MQ=255)
                                                                                                                         tGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCAtt                      <  1:2784975/148‑1 (MQ=255)
                                                                                                                           ggTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAAAAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGAACAAGAGCAATCg                    >  1:613449/1‑148 (MQ=255)
                                                                                                                            gTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGa                   <  2:2121434/148‑1 (MQ=255)
                                                                                                                            gTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGa                   >  2:1419873/1‑148 (MQ=255)
                                                                                                                             tCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGaa                  <  1:932262/148‑1 (MQ=255)
                                                                                                                                 cGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAatat              <  2:2782610/148‑1 (MQ=255)
                                                                                                                                 cGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAatat              <  2:227138/148‑1 (MQ=255)
                                                                                                                                     cGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCg          <  2:1519248/148‑1 (MQ=255)
                                                                                                                                       cTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGaa        >  2:223376/1‑148 (MQ=255)
                                                                                                                                         gTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGc      >  2:237677/1‑148 (MQ=255)
                                                                                                                                          ttCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCg     >  2:3193833/1‑148 (MQ=255)
                                                                                                                                          ttCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCg     >  1:81642/1‑148 (MQ=255)
                                                                                                                                          ttCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCg     >  2:830112/1‑148 (MQ=255)
                                                                                                                                          ttCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCg     >  1:723676/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGAT  >  CP009273/426012‑426300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑