Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 432,762 G→T 100% P277Q (CCG→CAG)  yajO ← 2‑carboxybenzaldehyde reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273432,7620GT98.6% 673.2 / 0.9 212P277Q (CCG→CAG) yajO2‑carboxybenzaldehyde reductase
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (112/97);  total (113/99)
Fisher's exact test for biased strand distribution p-value = 6.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 212 total aligned reads displayed.

TTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTC  >  CP009273/432617‑432906
                                                                                                                                                 |                                                                                                                                                
ttATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGt                                                                                                                                                <  1:1796773/148‑1 (MQ=255)
    ttAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTTATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAAACGGTGCGGCAATGCCCTGTTTAc                                                                                                                                            >  1:3796854/1‑148 (MQ=255)
    ttAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTAc                                                                                                                                            >  2:1447479/1‑148 (MQ=255)
     tAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACt                                                                                                                                           <  2:1686857/148‑1 (MQ=255)
       aaTCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCCATGCCCTGTTTACTca                                                                                                                                         >  1:368258/1‑148 (MQ=255)
       aaTCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTca                                                                                                                                         <  2:2532253/148‑1 (MQ=255)
       aaTCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTca                                                                                                                                         >  1:1521487/1‑148 (MQ=255)
       aaTCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCGGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTca                                                                                                                                         >  1:368338/1‑148 (MQ=255)
         tCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTCATcaac                                                                                                                                       >  2:3531946/1‑148 (MQ=255)
          ccTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTcaaca                                                                                                                                      >  2:1241152/1‑148 (MQ=255)
          ccTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTcaaca                                                                                                                                      <  1:1465697/148‑1 (MQ=255)
            tACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCaacaac                                                                                                                                    >  1:72884/1‑148 (MQ=255)
            tACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCaacaac                                                                                                                                    >  2:1535617/1‑148 (MQ=255)
            tACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCaacaac                                                                                                                                    <  2:3028539/148‑1 (MQ=255)
            tACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCaacaac                                                                                                                                    >  1:72937/1‑148 (MQ=255)
            tACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCaacaac                                                                                                                                    >  2:1869144/1‑148 (MQ=255)
                aCAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAgg                                                                                                                                >  1:3133981/1‑148 (MQ=255)
                 cAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGc                                                                                                                               >  2:2997119/1‑148 (MQ=255)
                   ggATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCa                                                                                                                             >  2:706343/1‑148 (MQ=255)
                    gATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAg                                                                                                                            >  1:1107971/1‑148 (MQ=255)
                      tGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAgcg                                                                                                                          <  1:2082914/148‑1 (MQ=255)
                          gTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAACTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAAc                                                                                                                      >  1:3656145/1‑148 (MQ=255)
                                   gCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCg                                                                                                             >  2:1100689/1‑148 (MQ=255)
                                      tttCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCgtgt                                                                                                          >  1:2264789/1‑148 (MQ=255)
                                      tttCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCgtgt                                                                                                          >  1:1699946/1‑148 (MQ=255)
                                      tttCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCgtgt                                                                                                          <  1:1021916/148‑1 (MQ=255)
                                             ttCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGccccc                                                                                                   >  1:2119925/1‑148 (MQ=255)
                                               cGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAg                                                                                                 <  2:1814342/148‑1 (MQ=255)
                                               cGGCAATCTGGTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAg                                                                                                 <  2:3223737/148‑1 (MQ=255)
                                                ggCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGt                                                                                                <  1:439823/148‑1 (MQ=255)
                                                  cAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGttc                                                                                              >  2:3317117/1‑148 (MQ=255)
                                                    aTCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGttctt                                                                                            <  2:2434098/148‑1 (MQ=255)
                                                    aTCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGttctt                                                                                            <  1:3093890/148‑1 (MQ=255)
                                                    aTCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGttctt                                                                                            <  2:638155/148‑1 (MQ=255)
                                                     tCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGttcttc                                                                                           >  1:2297505/1‑148 (MQ=255)
                                                      cTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCa                                                                                          >  1:618999/1‑148 (MQ=255)
                                                        gTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACt                                                                                        >  2:1712578/1‑148 (MQ=255)
                                                        gTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACt                                                                                        >  1:2038506/1‑148 (MQ=255)
                                                         ttCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTg                                                                                       <  2:2005708/148‑1 (MQ=255)
                                                             ggCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGc                                                                                   <  1:1366298/148‑1 (MQ=255)
                                                                 tCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGt                                                                               <  2:2230125/148‑1 (MQ=255)
                                                                   aaaGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGACAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTa                                                                             <  1:2692007/148‑1 (MQ=255)
                                                                      gTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAAcc                                                                          >  2:2013013/1‑148 (MQ=255)
                                                                       tGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCg                                                                         >  1:3557583/1‑148 (MQ=255)
                                                                         atatCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGct                                                                       <  2:2846795/148‑1 (MQ=255)
                                                                         atatCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGct                                                                       <  2:1191119/148‑1 (MQ=255)
                                                                         atatCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGct                                                                       >  2:2192587/1‑148 (MQ=255)
                                                                          tatCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGctc                                                                      >  2:275961/1‑148 (MQ=255)
                                                                            tCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTg                                                                    <  2:2660004/148‑1 (MQ=255)
                                                                            tCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTg                                                                    <  2:1160714/148‑1 (MQ=255)
                                                                              cACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCg                                                                  >  1:1150321/1‑148 (MQ=255)
                                                                               aCCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGa                                                                 <  2:1540414/148‑1 (MQ=255)
                                                                                 cgcgTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATc                                                               <  1:170793/148‑1 (MQ=255)
                                                                                 cgcgTTCAAGAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATc                                                               <  1:972058/148‑1 (MQ=255)
                                                                                   cgTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTg                                                             <  1:1844860/148‑1 (MQ=255)
                                                                                      tCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTgcgc                                                          >  1:1527008/1‑148 (MQ=255)
                                                                                      tCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTgcgc                                                          >  1:3427609/1‑148 (MQ=255)
                                                                                         aTAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTc                                                       <  1:675457/148‑1 (MQ=255)
                                                                                           aGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCAt                                                     <  1:2973061/148‑1 (MQ=255)
                                                                                            gCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCAtt                                                    >  1:283570/1‑148 (MQ=255)
                                                                                            gCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCAtt                                                    >  1:3408864/1‑148 (MQ=255)
                                                                                            gCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCAtt                                                    >  1:3289668/1‑148 (MQ=255)
                                                                                            gCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCAtt                                                    >  1:3273382/1‑148 (MQ=255)
                                                                                             cTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATACTCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCAttt                                                   <  1:661774/148‑1 (MQ=255)
                                                                                               catcaAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTc                                                 <  1:1743159/148‑1 (MQ=255)
                                                                                               catcaAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTc                                                 >  2:2624724/1‑148 (MQ=255)
                                                                                               catcaAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTc                                                 >  2:1721005/1‑148 (MQ=255)
                                                                                               catcaAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTc                                                 >  2:457637/1‑148 (MQ=255)
                                                                                                atcaAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCa                                                >  1:1019502/1‑148 (MQ=255)
                                                                                                 tcaAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCAt                                               >  1:2486798/1‑148 (MQ=255)
                                                                                                     gCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGct                                           <  2:2621307/148‑1 (MQ=255)
                                                                                                     gCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGct                                           >  1:2028375/1‑148 (MQ=255)
                                                                                                        gTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGctttc                                        <  2:3306799/148‑1 (MQ=255)
                                                                                                        gTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGctttc                                        <  2:786918/148‑1 (MQ=255)
                                                                                                        gTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGctttc                                        <  2:938772/148‑1 (MQ=255)
                                                                                                         ttcttcGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGctttct                                       >  1:3338676/1‑148 (MQ=255)
                                                                                                         ttcttcGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGctttct                                       >  2:2376178/1‑148 (MQ=255)
                                                                                                         ttcttcGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGctttct                                       >  2:1789993/1‑148 (MQ=255)
                                                                                                           cttcGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGctttcttt                                     <  1:1963119/148‑1 (MQ=255)
                                                                                                           cttcGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGctttctgt                                     >  2:80194/1‑146 (MQ=255)
                                                                                                             tcGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTtat                                   >  1:3170036/1‑148 (MQ=255)
                                                                                                               gcgcgAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATag                                 <  2:1150321/148‑1 (MQ=255)
                                                                                                                cgcgAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATaga                                >  2:1118598/1‑148 (MQ=255)
                                                                                                                 gcgAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATagag                               <  1:933004/148‑1 (MQ=255)
                                                                                                                 gcgAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATagag                               <  1:3465347/148‑1 (MQ=255)
                                                                                                                  cgAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAgaga                              >  2:3247224/1‑148 (MQ=255)
                                                                                                                    aaGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGAtt                            >  1:2350589/1‑148 (MQ=255)
                                                                                                                    aaGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGAtt                            <  1:2513603/148‑1 (MQ=255)
                                                                                                                    aaGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGAtt                            <  2:916993/148‑1 (MQ=255)
                                                                                                                    aaGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGAtt                            >  2:3396789/1‑148 (MQ=255)
                                                                                                                     aGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGAttt                           >  1:1083360/1‑148 (MQ=255)
                                                                                                                       ttCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGAttttt                         <  1:1700656/148‑1 (MQ=255)
                                                                                                                         ccGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTcc                       >  2:1333548/1‑148 (MQ=255)
                                                                                                                            aTAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCa                    >  2:2282777/1‑148 (MQ=255)
                                                                                                                              aaTCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCAcc                  >  2:3681632/1‑148 (MQ=255)
                                                                                                                                tCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCtc                <  2:751939/148‑1 (MQ=255)
                                                                                                                                tCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCtc                >  2:3124298/1‑148 (MQ=255)
                                                                                                                                 cGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCtca               >  2:2928523/1‑148 (MQ=255)
                                                                                                                                   gTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCtcatc             <  1:589377/148‑1 (MQ=255)
                                                                                                                                              cccTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTc  >  2:3364770/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTC  >  CP009273/432617‑432906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑