Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 435,336 G→T 100% Q100K (CAG→AAG)  dxs ← 1‑deoxyxylulose‑5‑phosphate synthase, thiamine triphosphate‑binding, FAD‑requiring

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273435,3360GT100.0% 654.6 / ‑6.7 196Q100K (CAG→AAG) dxs1‑deoxyxylulose‑5‑phosphate synthase, thiamine triphosphate‑binding, FAD‑requiring
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (101/94);  total (101/95)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 196 total aligned reads displayed.

GACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGCCCGG  >  CP009273/435193‑435479
                                                                                                                                               |                                                                                                                                               
gACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACgg                                                                                                                                             <  1:1292234/148‑1 (MQ=255)
gACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACgg                                                                                                                                             <  1:795990/148‑1 (MQ=255)
 aCGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACggc                                                                                                                                            >  2:3461526/1‑147 (MQ=255)
    gTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATgg                                                                                                                                         <  1:470505/148‑1 (MQ=255)
      gcggcgATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTg                                                                                                                                       <  2:1538887/148‑1 (MQ=255)
       cggcgATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGc                                                                                                                                      <  2:1059995/148‑1 (MQ=255)
       cggcgATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGc                                                                                                                                      <  2:1047540/148‑1 (MQ=255)
            aTTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGAtt                                                                                                                                 >  1:113508/1‑148 (MQ=255)
            aTTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGAtt                                                                                                                                 <  1:3258264/148‑1 (MQ=255)
             tttttGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGAttt                                                                                                                                <  1:3504728/148‑1 (MQ=255)
             tttttGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGAttt                                                                                                                                >  1:1631937/1‑148 (MQ=255)
                  gCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTcg                                                                                                                           <  2:515487/148‑1 (MQ=255)
                  gCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTcg                                                                                                                           <  2:347993/148‑1 (MQ=255)
                   ccTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTcgc                                                                                                                          >  1:1074242/1‑148 (MQ=255)
                   ccTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACCGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTcgc                                                                                                                          >  1:2982837/1‑148 (MQ=255)
                       cttTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCgcggcg                                                                                                                      >  2:1150031/1‑148 (MQ=255)
                           ttCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCg                                                                                                                  <  2:2418266/148‑1 (MQ=255)
                             cGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGt                                                                                                                <  1:2819646/148‑1 (MQ=255)
                             cGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGt                                                                                                                <  1:2077544/148‑1 (MQ=255)
                                      ccGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGACCGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAAtttt                                                                                                       >  2:1634033/1‑148 (MQ=255)
                                       cGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTa                                                                                                      <  1:3556938/148‑1 (MQ=255)
                                         cAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATg                                                                                                    <  1:672074/148‑1 (MQ=255)
                                         cAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATg                                                                                                    <  1:2685238/148‑1 (MQ=255)
                                           aTACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCg                                                                                                  >  2:1408138/1‑148 (MQ=255)
                                            tACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGACCGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCgg                                                                                                 >  2:3399057/1‑148 (MQ=255)
                                            tACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCgg                                                                                                 >  2:3578371/1‑148 (MQ=255)
                                            tACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCgg                                                                                                 >  2:1805486/1‑148 (MQ=255)
                                             aCCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGa                                                                                                >  1:1650245/1‑148 (MQ=255)
                                               cAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACTGGTGCAGACCGCCTTTCTTACGGATGGTGACGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATa                                                                                              >  2:3036040/1‑148 (MQ=255)
                                               cAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATa                                                                                              <  1:502707/148‑1 (MQ=255)
                                                aaTTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATaa                                                                                             >  2:2684175/1‑148 (MQ=255)
                                                aaTTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATaa                                                                                             <  1:255987/148‑1 (MQ=255)
                                                 aTTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAg                                                                                            >  2:1456060/1‑148 (MQ=255)
                                                 aTTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAg                                                                                            <  1:1040573/148‑1 (MQ=255)
                                                  ttCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGc                                                                                           <  2:1399138/148‑1 (MQ=255)
                                                  gtCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGc                                                                                           <  2:1962362/147‑1 (MQ=255)
                                                   tCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGcc                                                                                          <  1:2397020/148‑1 (MQ=255)
                                                     cGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTg                                                                                        <  1:567670/148‑1 (MQ=255)
                                                      ggCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGa                                                                                       <  1:2953885/148‑1 (MQ=255)
                                                      ggCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGa                                                                                       <  1:76542/148‑1 (MQ=255)
                                                        cACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATg                                                                                     >  1:969323/1‑148 (MQ=255)
                                                        cACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATg                                                                                     >  1:3387368/1‑148 (MQ=255)
                                                          cTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGcc                                                                                   <  2:905099/148‑1 (MQ=255)
                                                           tGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGccc                                                                                  <  1:1995749/148‑1 (MQ=255)
                                                            gATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGcccc                                                                                 >  2:2854272/1‑148 (MQ=255)
                                                             aTGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCca                                                                                >  1:1833824/1‑148 (MQ=255)
                                                               ggAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCcaca                                                                              >  1:1318992/1‑148 (MQ=255)
                                                               ggAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCcaca                                                                              >  1:1160566/1‑148 (MQ=255)
                                                                gAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACAt                                                                             <  1:1862164/148‑1 (MQ=255)
                                                                gAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACAt                                                                             <  1:1016373/148‑1 (MQ=255)
                                                                gAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACAt                                                                             <  2:1000950/148‑1 (MQ=255)
                                                                 aGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATc                                                                            >  1:480359/1‑148 (MQ=255)
                                                                  ggTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATcc                                                                           <  2:722177/148‑1 (MQ=255)
                                                                  ggTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATcc                                                                           <  2:1883680/148‑1 (MQ=255)
                                                                         gaATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCa                                                                    >  1:1123522/1‑148 (MQ=255)
                                                                         gaATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCa                                                                    >  1:1915666/1‑148 (MQ=255)
                                                                          aaTGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCaa                                                                   <  1:2900629/148‑1 (MQ=255)
                                                                          aaTGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCaa                                                                   >  1:336560/1‑148 (MQ=255)
                                                                                   cGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCaaa                                                          >  2:66562/1‑148 (MQ=255)
                                                                                   cGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCaaa                                                          >  2:728591/1‑148 (MQ=255)
                                                                                     cTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACg                                                        >  2:1169469/1‑148 (MQ=255)
                                                                                     cTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACg                                                        <  2:2774835/148‑1 (MQ=255)
                                                                                      ttAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACgg                                                       <  1:2192041/148‑1 (MQ=255)
                                                                                       tAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACggg                                                      >  2:2304026/1‑148 (MQ=255)
                                                                                       tAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAAGTGGTCAAACggg                                                      >  1:307582/1‑148 (MQ=255)
                                                                                         aTACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGgt                                                    >  2:2528183/1‑148 (MQ=255)
                                                                                         aTACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGgt                                                    >  2:2529695/1‑148 (MQ=255)
                                                                                          tACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGgtg                                                   <  1:1696112/148‑1 (MQ=255)
                                                                                          tACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGgtg                                                   >  1:804584/1‑148 (MQ=255)
                                                                                          tACGTCATAGTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGgtg                                                   <  2:1952828/148‑1 (MQ=255)
                                                                                           aCGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGgtgt                                                  >  2:1222248/1‑148 (MQ=255)
                                                                                               cATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTa                                              >  2:2064894/1‑148 (MQ=255)
                                                                                                atatTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAg                                             <  1:1053829/148‑1 (MQ=255)
                                                                                                 tatTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGa                                            >  2:2627064/1‑148 (MQ=255)
                                                                                                 tatTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGa                                            >  2:2662030/1‑148 (MQ=255)
                                                                                                  atTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGAc                                           >  2:1654091/1‑148 (MQ=255)
                                                                                                  atTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGAc                                           <  2:3358729/148‑1 (MQ=255)
                                                                                                      tcTTTCGCCGCGACACGGGAACGTGTGCAGACCGCCTTTCTTACGGATTGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAg                                       <  1:3489169/147‑1 (MQ=255)
                                                                                                        tttCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTg                                     >  2:1938515/1‑148 (MQ=255)
                                                                                                         ttCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGc                                    >  1:6895/1‑148 (MQ=255)
                                                                                                          tCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCa                                   <  2:1631937/148‑1 (MQ=255)
                                                                                                                       ggAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAg                      <  1:906343/148‑1 (MQ=255)
                                                                                                                        gAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGt                     <  1:1000527/148‑1 (MQ=255)
                                                                                                                        gAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGt                     >  1:252113/1‑148 (MQ=255)
                                                                                                                        gAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGt                     >  2:2861618/1‑148 (MQ=255)
                                                                                                                        gAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGt                     >  2:609183/1‑148 (MQ=255)
                                                                                                                         aaCGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGtt                    >  2:1355046/1‑148 (MQ=255)
                                                                                                                          aCGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTc                   >  1:334740/1‑148 (MQ=255)
                                                                                                                          aCGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTc                   >  1:1581865/1‑148 (MQ=255)
                                                                                                                               tGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTTGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCg              >  1:326668/1‑148 (MQ=255)
                                                                                                                               tGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGGCAGTTCGACCg              >  1:324664/1‑148 (MQ=255)
                                                                                                                                gCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGt             >  1:2466597/1‑148 (MQ=255)
                                                                                                                                   gACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGcc          >  2:212738/1‑148 (MQ=255)
                                                                                                                                   gACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGcc          >  2:3674010/1‑148 (MQ=255)
                                                                                                                                   gACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGcc          >  2:2368617/1‑148 (MQ=255)
                                                                                                                                    aCCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGccc         >  1:3378020/1‑148 (MQ=255)
                                                                                                                                         cTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGccc    >  1:1618826/1‑148 (MQ=255)
                                                                                                                                          tttCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGCCCg   >  1:3024850/1‑148 (MQ=255)
                                                                                                                                          tttCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGCCCg   >  1:2703490/1‑148 (MQ=255)
                                                                                                                                           ttCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGCCCgg  <  1:889662/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                               
GACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGCCCGG  >  CP009273/435193‑435479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑