Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 444,489 G→A 100% R537C (CGT→TGT)  cyoB ← cytochrome o ubiquinol oxidase subunit I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273444,4890GA100.0% 846.9 / NA 252R537C (CGT→TGT) cyoBcytochrome o ubiquinol oxidase subunit I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (137/115);  total (137/115)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 252 total aligned reads displayed.

TATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTG  >  CP009273/444345‑444630
                                                                                                                                                |                                                                                                                                             
tatGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGcc                                                                                                                                            >  1:2295737/1‑148 (MQ=255)
 atGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCa                                                                                                                                           <  1:1296172/148‑1 (MQ=255)
 atGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGACCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAATAGTTTGCGCACTCCAGCGTAAAGCCa                                                                                                                                           >  2:1457608/1‑148 (MQ=255)
  tGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCAc                                                                                                                                          >  1:3076703/1‑148 (MQ=255)
      tttCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCa                                                                                                                                      >  2:157189/1‑148 (MQ=255)
      tttCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCa                                                                                                                                      >  1:3113265/1‑148 (MQ=255)
      tttCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCa                                                                                                                                      >  1:783753/1‑148 (MQ=255)
      tttCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCa                                                                                                                                      >  2:3671266/1‑148 (MQ=255)
      tttCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCa                                                                                                                                      >  1:1637443/1‑148 (MQ=255)
       ttcttcATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCAc                                                                                                                                     >  1:1740430/1‑148 (MQ=255)
         cttcATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACgg                                                                                                                                   >  1:555467/1‑148 (MQ=255)
           tcATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGt                                                                                                                                 >  2:3126303/1‑148 (MQ=255)
           tcATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGt                                                                                                                                 >  2:1141374/1‑148 (MQ=255)
                 tGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAg                                                                                                                           >  1:643237/1‑148 (MQ=255)
                  ggTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGt                                                                                                                          <  1:3105525/148‑1 (MQ=255)
                   gTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTc                                                                                                                         >  1:2798157/1‑148 (MQ=255)
                    tCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTTCGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCGAGTCa                                                                                                                        <  2:3642871/148‑1 (MQ=255)
                     cAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAg                                                                                                                       >  2:3494906/1‑148 (MQ=255)
                      aGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAgg                                                                                                                      >  1:2646620/1‑148 (MQ=255)
                      aGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAgg                                                                                                                      >  2:3072717/1‑148 (MQ=255)
                           ttttATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACg                                                                                                                 <  2:3785593/148‑1 (MQ=255)
                              tataCGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGtt                                                                                                              >  2:2428559/1‑148 (MQ=255)
                              tataCGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGtt                                                                                                              >  1:2478880/1‑148 (MQ=255)
                               ataCGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTc                                                                                                             <  1:1682596/148‑1 (MQ=255)
                                  cGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTgg                                                                                                          >  2:3754827/1‑148 (MQ=255)
                                       cGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcgcg                                                                                                     >  2:2268171/1‑148 (MQ=255)
                                          cTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcgcggtc                                                                                                  >  1:2549768/1‑148 (MQ=255)
                                           tttCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcgcggtcg                                                                                                 >  1:3779844/1‑148 (MQ=255)
                                           tttCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcgcggtcg                                                                                                 <  2:957623/148‑1 (MQ=255)
                                           tttCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcgcggtcg                                                                                                 >  1:1168036/1‑148 (MQ=255)
                                            ttCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcgcggtcgc                                                                                                >  1:887521/1‑148 (MQ=255)
                                             tctcTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGgtcgcggtcgcg                                                                                               >  1:371145/1‑148 (MQ=255)
                                             tctcTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGgtcgcggtcgcg                                                                                               >  2:3259321/1‑148 (MQ=255)
                                               tctTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGaa                                                                                             <  1:1340211/148‑1 (MQ=255)
                                                      tttCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAAc                                                                                      >  2:1805759/1‑148 (MQ=255)
                                                       ttCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGACACg                                                                                     >  2:2615854/1‑148 (MQ=255)
                                                       ttCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACg                                                                                     >  2:1611867/1‑148 (MQ=255)
                                                        tCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGt                                                                                    <  1:3565878/148‑1 (MQ=255)
                                                        tCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGt                                                                                    <  2:3241937/148‑1 (MQ=255)
                                                          ccAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTAc                                                                                  <  1:3671266/148‑1 (MQ=255)
                                                           cAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACa                                                                                 <  1:292488/148‑1 (MQ=255)
                                                           cAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACa                                                                                 <  1:292480/148‑1 (MQ=255)
                                                             gAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGACTAGAAACGTACATc                                                                               >  2:2419707/1‑148 (MQ=255)
                                                             gAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATc                                                                               >  1:3271055/1‑148 (MQ=255)
                                                             gAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATc                                                                               >  2:1876947/1‑148 (MQ=255)
                                                              aaTGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCt                                                                              >  2:1919566/1‑148 (MQ=255)
                                                              aaTGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCt                                                                              >  1:1961563/1‑148 (MQ=255)
                                                                tGCATCACGTTGGTGTACGTGCTGCACTCCGGCAAAGTTATAGTACGGAGGCGGGGAAGAGGTTGCCCACTCCATCGTACAGCGACCCCACGGGTCGCCAGTCAGGTCACGGGTCTGGTCGGGGTCGCGAATAGAAACGTACATCTGa                                                                            <  2:1243075/148‑1 (MQ=255)
                                                                tGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGa                                                                            >  2:2839227/1‑148 (MQ=255)
                                                                 gCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGaa                                                                           <  1:2044309/148‑1 (MQ=255)
                                                                 gCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAAGCGAACCGTACATCTGaa                                                                           >  2:2082082/1‑148 (MQ=255)
                                                                   aTCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATa                                                                         <  2:1194675/148‑1 (MQ=255)
                                                                   aTCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATa                                                                         <  1:3024874/148‑1 (MQ=255)
                                                                   aTCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATa                                                                         <  1:380892/148‑1 (MQ=255)
                                                                      aCGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACg                                                                      <  1:2293330/148‑1 (MQ=255)
                                                                       cGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGa                                                                     <  1:863772/148‑1 (MQ=255)
                                                                       cGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGa                                                                     <  1:3494906/148‑1 (MQ=255)
                                                                       cGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGa                                                                     >  2:1268782/1‑148 (MQ=255)
                                                                        gTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAg                                                                    >  2:1023778/1‑148 (MQ=255)
                                                                          tCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGc                                                                  >  2:2246730/1‑148 (MQ=255)
                                                                           cGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCa                                                                 <  1:409388/148‑1 (MQ=255)
                                                                           cGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCa                                                                 <  1:409353/148‑1 (MQ=255)
                                                                            gTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCag                                                                <  1:3595428/148‑1 (MQ=255)
                                                                            gTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCag                                                                <  1:179419/148‑1 (MQ=255)
                                                                               aaCGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAgaga                                                             <  2:3622934/148‑1 (MQ=255)
                                                                                 cGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAAt                                                           <  1:3647322/148‑1 (MQ=255)
                                                                                 cGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAAt                                                           <  1:693635/148‑1 (MQ=255)
                                                                                     cGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATAccc                                                       <  2:1590420/148‑1 (MQ=255)
                                                                                       gCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAg                                                     <  2:3183049/148‑1 (MQ=255)
                                                                                           tACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCa                                                 <  1:211538/148‑1 (MQ=255)
                                                                                            aCGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCaa                                                <  2:783826/148‑1 (MQ=255)
                                                                                              ggCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATc                                              <  1:2525227/148‑1 (MQ=255)
                                                                                              ggCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATc                                              <  2:371145/148‑1 (MQ=255)
                                                                                                 aaaGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGt                                           <  1:2007136/148‑1 (MQ=255)
                                                                                                 aaaGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGt                                           <  1:1014435/148‑1 (MQ=255)
                                                                                                 aaaGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGt                                           <  1:1537897/148‑1 (MQ=255)
                                                                                                 aaaGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGt                                           <  1:1172469/148‑1 (MQ=255)
                                                                                                   aGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAc                                         >  1:563987/1‑148 (MQ=255)
                                                                                                      tataGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGc                                      >  1:1149962/1‑148 (MQ=255)
                                                                                                      tataGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGc                                      >  1:1873124/1‑148 (MQ=255)
                                                                                                        taGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCAc                                    >  1:1917728/1‑148 (MQ=255)
                                                                                                          gAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCg                                  <  2:643347/148‑1 (MQ=255)
                                                                                                           aaCGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCgc                                 >  2:3672008/1‑148 (MQ=255)
                                                                                                              ggAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCgctgg                              <  1:2371416/148‑1 (MQ=255)
                                                                                                                  gCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGc                          >  2:2809049/1‑148 (MQ=255)
                                                                                                                    ggggAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCaa                        <  1:3705455/148‑1 (MQ=255)
                                                                                                                     gggAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGACACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAat                       >  2:2642729/1‑148 (MQ=255)
                                                                                                                     gggAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAat                       <  2:590388/148‑1 (MQ=255)
                                                                                                                      ggAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAatc                      >  1:1205426/1‑148 (MQ=255)
                                                                                                                      ggAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAatc                      >  1:3429312/1‑148 (MQ=255)
                                                                                                                      ggAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAatc                      >  1:1327280/1‑148 (MQ=255)
                                                                                                                        aaGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAatcat                    <  2:2960391/148‑1 (MQ=255)
                                                                                                                             gTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCa               >  2:3400546/1‑148 (MQ=255)
                                                                                                                               tGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATg             <  2:2015416/148‑1 (MQ=255)
                                                                                                                                  ccACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGgtg          <  1:1805774/148‑1 (MQ=255)
                                                                                                                                    aCTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGtgtg        <  2:2351078/148‑1 (MQ=255)
                                                                                                                                     cTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTgg       >  1:3470178/1‑148 (MQ=255)
                                                                                                                                       ccAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGaa     <  2:555465/148‑1 (MQ=255)
                                                                                                                                         aGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACt   >  2:393858/1‑148 (MQ=255)
                                                                                                                                          gCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTg  <  1:2785553/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                             
TATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTG  >  CP009273/444345‑444630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑