Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 450,766 G→T 100% A60S (GCG→TCG)  tig → peptidyl‑prolyl cis/trans isomerase (trigger factor)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273450,7660GT99.0% 645.5 / ‑3.2 195A60S (GCG→TCG) tigpeptidyl‑prolyl cis/trans isomerase (trigger factor)
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base T (92/101);  total (94/101)
Fisher's exact test for biased strand distribution p-value = 2.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 197 total aligned reads displayed.

TGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTGTAG  >  CP009273/450621‑450910
                                                                                                                                                 |                                                                                                                                                
tGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGctcg                                                                                                                                                >  2:1900976/1‑148 (MQ=255)
  gccgccGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTc                                                                                                                                              <  2:1082349/148‑1 (MQ=255)
  gccgccGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTc                                                                                                                                              <  2:2989380/148‑1 (MQ=255)
  gccgccGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTc                                                                                                                                              <  2:3150875/148‑1 (MQ=255)
      ccgTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTa                                                                                                                                          >  2:2190015/1‑148 (MQ=255)
      ccgTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTa                                                                                                                                          <  2:2830883/148‑1 (MQ=255)
           tAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCa                                                                                                                                     <  1:2962605/148‑1 (MQ=255)
            aaCGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAg                                                                                                                                    >  2:3553005/1‑148 (MQ=255)
              cGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGa                                                                                                                                  >  2:1275045/1‑148 (MQ=255)
                 ttACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGt                                                                                                                               <  2:1725432/148‑1 (MQ=255)
                 ttACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGt                                                                                                                               >  1:3414046/1‑148 (MQ=255)
                 ttACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGt                                                                                                                               <  1:939089/148‑1 (MQ=255)
                   aCTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTc                                                                                                                             >  2:1513693/1‑148 (MQ=255)
                     tATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTg                                                                                                                           >  2:2647018/1‑148 (MQ=255)
                          ctgctgACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGACAGGACGTTCTGGGTGa                                                                                                                      <  1:3268700/148‑1 (MQ=255)
                                   gCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAg                                                                                                             <  1:177935/148‑1 (MQ=255)
                                   gCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAg                                                                                                             <  1:2107782/148‑1 (MQ=255)
                                    cATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGc                                                                                                            >  2:3244147/1‑148 (MQ=255)
                                      tCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCg                                                                                                          >  2:2497221/1‑148 (MQ=255)
                                       cGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGt                                                                                                         >  2:2069403/1‑148 (MQ=255)
                                           aCCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACt                                                                                                     >  2:861597/1‑148 (MQ=255)
                                            ccGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACtt                                                                                                    >  1:2382285/1‑148 (MQ=255)
                                             cGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGGTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTc                                                                                                   >  1:3011545/1‑148 (MQ=255)
                                               cTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCAt                                                                                                 <  2:2147000/148‑1 (MQ=255)
                                                 gTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTg                                                                                               <  1:2778865/148‑1 (MQ=255)
                                                 gTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTg                                                                                               <  1:864167/148‑1 (MQ=255)
                                                 gTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTg                                                                                               <  1:518767/148‑1 (MQ=255)
                                                     aaaGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGc                                                                                           <  2:1856871/148‑1 (MQ=255)
                                                      aaGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGcc                                                                                          >  2:1671475/1‑148 (MQ=255)
                                                      aaGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGcc                                                                                          >  2:293652/1‑148 (MQ=255)
                                                             cTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTa                                                                                   <  2:1574346/148‑1 (MQ=255)
                                                             cTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTa                                                                                   <  2:1440369/148‑1 (MQ=255)
                                                               ggTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTaaa                                                                                 <  1:3300574/148‑1 (MQ=255)
                                                                    aCGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGaaaa                                                                            <  2:1408861/148‑1 (MQ=255)
                                                                     cGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGaaaaa                                                                           >  2:2077101/1‑148 (MQ=255)
                                                                      gTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGaaaaaa                                                                          <  2:3414046/148‑1 (MQ=255)
                                                                         gCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAaatca                                                                       <  2:1707680/148‑1 (MQ=255)
                                                                         gCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAaatca                                                                       <  1:928692/148‑1 (MQ=255)
                                                                         gCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAaatca                                                                       <  1:2520054/148‑1 (MQ=255)
                                                                         gCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAaatca                                                                       <  2:406966/148‑1 (MQ=255)
                                                                          cGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAaatcaa                                                                      <  2:1380729/148‑1 (MQ=255)
                                                                           gAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAaatcaat                                                                     <  2:730163/148‑1 (MQ=255)
                                                                            aaaaaaGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAaatcaatc                                                                    >  1:1043521/1‑148 (MQ=255)
                                                                              aaaaGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCg                                                                  >  1:2477370/1‑148 (MQ=255)
                                                                               aaaGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCgg                                                                 >  2:1130549/1‑148 (MQ=255)
                                                                                   tACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTgg                                                             >  1:1101026/1‑148 (MQ=255)
                                                                                       tATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCa                                                         <  1:3178029/148‑1 (MQ=255)
                                                                                       tATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCa                                                         >  2:1691273/1‑148 (MQ=255)
                                                                                        aTTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCAc                                                        >  1:836012/1‑148 (MQ=255)
                                                                                            aCGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGAc                                                    <  1:2518178/148‑1 (MQ=255)
                                                                                              ggCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACtt                                                  <  2:1371411/148‑1 (MQ=255)
                                                                                              ggCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACtt                                                  <  2:1188576/148‑1 (MQ=255)
                                                                                              ggCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACtt                                                  <  1:2981005/148‑1 (MQ=255)
                                                                                               gCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTa                                                 >  1:3757458/1‑148 (MQ=255)
                                                                                                   ccGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGtt                                             <  1:2457163/148‑1 (MQ=255)
                                                                                                    cGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTc                                            <  2:542943/148‑1 (MQ=255)
                                                                                                    cGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTc                                            <  2:3082457/148‑1 (MQ=255)
                                                                                                     gcaaaggcaaagTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTcc                                           >  2:3252445/1‑148 (MQ=255)
                                                                                                         aggcaaagTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGc                                       >  2:2374937/1‑148 (MQ=255)
                                                                                                         aggcaaagTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGc                                       >  2:3157648/1‑148 (MQ=255)
                                                                                                         aggcaaagTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGc                                       >  2:3722172/1‑148 (MQ=255)
                                                                                                         aggcaaagTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGc                                       >  2:665029/1‑148 (MQ=255)
                                                                                                          ggcaaagTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCg                                      >  2:3628857/1‑148 (MQ=255)
                                                                                                           gcaaagTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGa                                     <  1:1914829/148‑1 (MQ=255)
                                                                                                              aagTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATa                                  >  1:1823488/1‑148 (MQ=255)
                                                                                                              aagTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATa                                  >  1:3147697/1‑148 (MQ=255)
                                                                                                              aagTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATa                                  >  2:2704503/1‑148 (MQ=255)
                                                                                                                gTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACa                                <  1:386927/148‑1 (MQ=255)
                                                                                                                 tGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACaa                               <  1:1312670/148‑1 (MQ=255)
                                                                                                                  gCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAg                              <  1:2247389/148‑1 (MQ=255)
                                                                                                                    cAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCt                            >  1:2053064/1‑148 (MQ=255)
                                                                                                                     aaTGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTg                           >  2:1236964/1‑148 (MQ=255)
                                                                                                                      aTGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTgg                          >  1:1045086/1‑148 (MQ=255)
                                                                                                                      aTGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTgg                          >  2:2320412/1‑148 (MQ=255)
                                                                                                                       tGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTggg                         >  1:48323/1‑148 (MQ=255)
                                                                                                                        gAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGt                        <  2:1785348/148‑1 (MQ=255)
                                                                                                                        gAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGt                        >  2:3498444/1‑148 (MQ=255)
                                                                                                                          atatCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGa                      >  2:2976183/1‑148 (MQ=255)
                                                                                                                          atatCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGa                      <  1:17243/148‑1 (MQ=255)
                                                                                                                          atatCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGa                      <  1:3553005/148‑1 (MQ=255)
                                                                                                                           tatCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGaa                     >  1:2854009/1‑148 (MQ=255)
                                                                                                                           tatCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGaa                     >  1:1861318/1‑148 (MQ=255)
                                                                                                                            atCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAg                    <  2:530501/148‑1 (MQ=255)
                                                                                                                                ttGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACtt                <  2:1045086/148‑1 (MQ=255)
                                                                                                                                 tGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTc               <  1:1507497/148‑1 (MQ=255)
                                                                                                                                 tGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTc               <  1:1084991/148‑1 (MQ=255)
                                                                                                                                  gCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCa              >  1:623641/1‑148 (MQ=255)
                                                                                                                                  gCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCa              <  1:1233992/148‑1 (MQ=255)
                                                                                                                                   cTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCAc             >  2:3325903/1‑148 (MQ=255)
                                                                                                                                   cTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCAc             <  1:2275017/148‑1 (MQ=255)
                                                                                                                                   cTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCAc             <  2:836012/148‑1 (MQ=255)
                                                                                                                                     cAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACtt           >  1:3619611/1‑148 (MQ=255)
                                                                                                                                     cAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACtt           >  2:754112/1‑148 (MQ=255)
                                                                                                                                     cAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACtt           >  2:800947/1‑148 (MQ=255)
                                                                                                                                      aGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTa          <  1:3714893/148‑1 (MQ=255)
                                                                                                                                         gTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTActc       <  1:1671475/148‑1 (MQ=255)
                                                                                                                                          ttATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTActct      >  1:850843/1‑148 (MQ=255)
                                                                                                                                           tATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTg     >  1:98702/1‑148 (MQ=255)
                                                                                                                                           tATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTg     >  2:267077/1‑148 (MQ=255)
                                                                                                                                            aTGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTGt    >  1:3250592/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTGTAG  >  CP009273/450621‑450910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑