Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 456,588 G→T 100% D749Y (GAC→TAC)  lon → DNA‑binding ATP‑dependent protease La

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273456,5880GT100.0% 808.7 / NA 241D749Y (GAC→TAC) lonDNA‑binding ATP‑dependent protease La
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (136/105);  total (136/105)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 241 total aligned reads displayed.

TGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGG  >  CP009273/456443‑456732
                                                                                                                                                 |                                                                                                                                                
 gtgGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACa                                                                                                                                               <  1:835911/148‑1 (MQ=255)
    gCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACg                                                                                                                                            <  2:197456/148‑1 (MQ=255)
        tGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAAt                                                                                                                                        <  1:1042795/148‑1 (MQ=255)
        tGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAAt                                                                                                                                        <  1:2951898/148‑1 (MQ=255)
               tGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGAt                                                                                                                                 <  2:909257/148‑1 (MQ=255)
               tGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGAt                                                                                                                                 <  2:339191/148‑1 (MQ=255)
               tGAGATCACTCGGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGAt                                                                                                                                 <  2:1446654/148‑1 (MQ=255)
                gagaTCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATc                                                                                                                                <  1:976653/148‑1 (MQ=255)
                  gaTCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTg                                                                                                                              <  1:3337227/148‑1 (MQ=255)
                   aTCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTgg                                                                                                                             <  2:2344344/148‑1 (MQ=255)
                   aTCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTgg                                                                                                                             <  2:2491389/148‑1 (MQ=255)
                    tCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGa                                                                                                                            >  2:2421905/1‑148 (MQ=255)
                      aCTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACa                                                                                                                          >  1:2701169/1‑148 (MQ=255)
                        tctGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACAtt                                                                                                                        <  2:2939524/148‑1 (MQ=255)
                        tctGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACAtt                                                                                                                        <  2:2072861/148‑1 (MQ=255)
                         ctGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTc                                                                                                                       >  2:1366951/1‑148 (MQ=255)
                         ctGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTc                                                                                                                       >  1:3267386/1‑148 (MQ=255)
                           gCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCAt                                                                                                                     <  1:744193/148‑1 (MQ=255)
                           gCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCAt                                                                                                                     <  1:1393945/148‑1 (MQ=255)
                            cGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATc                                                                                                                    <  2:1794751/148‑1 (MQ=255)
                               ggTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCtg                                                                                                                 <  2:2893098/148‑1 (MQ=255)
                                gTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCtgt                                                                                                                <  2:3322353/148‑1 (MQ=255)
                                   aGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGaa                                                                                                             >  1:1783921/1‑148 (MQ=255)
                                    ggTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAg                                                                                                            <  2:3483073/148‑1 (MQ=255)
                                     gTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGCTTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAgc                                                                                                           >  1:3034747/1‑148 (MQ=255)
                                          gCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCAtt                                                                                                      >  2:2042035/1‑148 (MQ=255)
                                           ccGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTg                                                                                                     >  2:1522997/1‑148 (MQ=255)
                                               tCGGTGGTTTTTAAGTAAAACTCCTGGCAGCGCATTGCGGGGGTATTAAAACATTGCTAATTCCTTTCTAAAATAAACGCGATCTGGAAGAGAGTCCTTACTTCGTAATTGCCGTTCTGGTCATTCATCCTGTGAAGCGCATTGAgta                                                                                                 <  2:217758/148‑3 (MQ=255)
                                                   tggtTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGtt                                                                                             >  2:3287536/1‑148 (MQ=255)
                                                     gtTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCt                                                                                           <  1:3701081/148‑1 (MQ=255)
                                                      tttGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACCTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTg                                                                                          >  1:3726517/1‑148 (MQ=255)
                                                      tttGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTAGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATAGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTg                                                                                          >  1:1174195/1‑148 (MQ=255)
                                                       ttGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGACGCGCATTGAGGAAGTTCTGa                                                                                         >  2:1270558/1‑148 (MQ=255)
                                                       ttGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGa                                                                                         >  1:1417274/1‑148 (MQ=255)
                                                       ttGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGa                                                                                         >  2:2824693/1‑148 (MQ=255)
                                                         gaaagaaaAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGAct                                                                                       <  1:888078/148‑1 (MQ=255)
                                                         gaaagaaaAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGAct                                                                                       >  2:705792/1‑148 (MQ=255)
                                                          aaagaaaAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGActc                                                                                      <  2:3691116/148‑1 (MQ=255)
                                                             gaaaAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTgg                                                                                   >  2:339686/1‑148 (MQ=255)
                                                             gaaaAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTgg                                                                                   >  1:3235856/1‑148 (MQ=255)
                                                              aaaaaCTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGgc                                                                                  >  1:234942/1‑148 (MQ=255)
                                                               aaaaCTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGgcg                                                                                 >  2:1928098/1‑148 (MQ=255)
                                                               aaaaCTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGgcg                                                                                 <  1:1555701/148‑1 (MQ=255)
                                                                     ccTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCaa                                                                           >  2:2885949/1‑148 (MQ=255)
                                                                      cTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCaaa                                                                          <  1:2266623/148‑1 (MQ=255)
                                                                       tGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCaaaa                                                                         >  1:3478462/1‑148 (MQ=255)
                                                                         gCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATg                                                                       >  2:1964334/1‑148 (MQ=255)
                                                                         gCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATg                                                                       >  2:1577852/1‑148 (MQ=255)
                                                                          cAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGa                                                                      <  1:3265952/148‑1 (MQ=255)
                                                                          cAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGa                                                                      <  2:3267384/148‑1 (MQ=255)
                                                                           aGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGTTCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGAAATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGaa                                                                     <  1:1205723/148‑1 (MQ=255)
                                                                           aGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGaa                                                                     <  2:468866/148‑1 (MQ=255)
                                                                               cATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGt                                                                 <  2:678265/148‑1 (MQ=255)
                                                                                aTCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGACGTTCTGACTCTGGCGCTGCAAAATGAACCGTc                                                                >  2:3023912/1‑148 (MQ=255)
                                                                                aTCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTc                                                                <  1:1832359/148‑1 (MQ=255)
                                                                                 tCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCt                                                               <  2:1562566/148‑1 (MQ=255)
                                                                                   gcggcgGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTgg                                                             <  1:2261299/148‑1 (MQ=255)
                                                                                           aTTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAgg                                                     <  2:2753807/148‑1 (MQ=255)
                                                                                           aTTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAgg                                                     >  1:2638289/1‑148 (MQ=255)
                                                                                             tAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGtt                                                   >  2:449979/1‑148 (MQ=255)
                                                                                              aaaaCAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTtg                                                  <  1:1855145/148‑1 (MQ=255)
                                                                                              aaaaCAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTtg                                                  <  2:357337/148‑1 (MQ=255)
                                                                                               aaaCAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTtgt                                                 >  2:3241425/1‑148 (MQ=255)
                                                                                               aaaCAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTtgt                                                 >  2:3016978/1‑148 (MQ=255)
                                                                                                aaCAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTtgtg                                                >  1:2705882/1‑148 (MQ=255)
                                                                                                aaCAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTtgtg                                                >  2:1709237/1‑148 (MQ=255)
                                                                                                 aCAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGa                                               <  2:1886334/148‑1 (MQ=255)
                                                                                                 aCAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGa                                               <  2:2677722/148‑1 (MQ=255)
                                                                                                  cAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGAc                                              <  2:2817418/148‑1 (MQ=255)
                                                                                                  cAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGAc                                              >  2:1237839/1‑148 (MQ=255)
                                                                                                  cAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGAc                                              >  2:688536/1‑148 (MQ=255)
                                                                                                        tAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCaaa                                        <  2:2389334/148‑1 (MQ=255)
                                                                                                        tAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCaaa                                        <  2:17674/148‑1 (MQ=255)
                                                                                                         aaTTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCaaaa                                                                         >  1:2150532/1‑114 (MQ=255)
                                                                                                         aaTTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCaaaa                                       >  2:164726/1‑148 (MQ=255)
                                                                                                         aaTTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCaaaa                                       >  2:1244652/1‑148 (MQ=255)
                                                                                                         aaTTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCaaaa                                       >  1:924859/1‑148 (MQ=255)
                                                                                                           gtCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATa                                     <  2:882159/147‑1 (MQ=255)
                                                                                                                ttCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGAc                                >  2:2209530/1‑148 (MQ=255)
                                                                                                                ttCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGAc                                <  1:3554566/148‑1 (MQ=255)
                                                                                                                    aaaaTAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTcg                            >  2:567876/1‑148 (MQ=255)
                                                                                                                       aTAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCgcgc                         >  2:613070/1‑148 (MQ=255)
                                                                                                                       aTAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCgcgc                         >  1:3305268/1‑148 (MQ=255)
                                                                                                                        tAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCa                        >  1:3099073/1‑148 (MQ=255)
                                                                                                                           aCGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCaaaa                     <  1:2659204/148‑1 (MQ=255)
                                                                                                                           aCGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCaaaa                     >  1:1272590/1‑148 (MQ=255)
                                                                                                                              cgATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGc                  <  1:705792/148‑1 (MQ=255)
                                                                                                                               gATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCa                 <  2:3522125/148‑1 (MQ=255)
                                                                                                                                aTCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCAc                <  1:1477475/148‑1 (MQ=255)
                                                                                                                                  cTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACta              >  1:2057902/1‑148 (MQ=255)
                                                                                                                                   tGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACtaa             <  1:470665/148‑1 (MQ=255)
                                                                                                                                    ggAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACtaat            >  1:3654319/1‑148 (MQ=255)
                                                                                                                                    ggAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACtaat            >  1:582693/1‑148 (MQ=255)
                                                                                                                                     gAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACtaata           >  2:260283/1‑148 (MQ=255)
                                                                                                                                     gAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACtaata           >  2:1050177/1‑148 (MQ=255)
                                                                                                                                           aTTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACa     >  2:3203571/1‑148 (MQ=255)
                                                                                                                                            ttCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAg    <  1:3302520/148‑1 (MQ=255)
                                                                                                                                            ttCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAg    <  1:1006143/148‑1 (MQ=255)
                                                                                                                                             tCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAgg   >  2:359901/1‑148 (MQ=255)
                                                                                                                                              ccTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAggg  >  1:1228048/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGG  >  CP009273/456443‑456732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑