Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 54,392 G→T 100% A104E (GCG→GAG)  surA ← peptidyl‑prolyl cis‑trans isomerase (PPIase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927354,3920GT100.0% 673.0 / NA 199A104E (GCG→GAG) surApeptidyl‑prolyl cis‑trans isomerase (PPIase)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (109/90);  total (109/90)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 201 total aligned reads displayed.

GATGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCGCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTT  >  CP009273/54247‑54535
                                                                                                                                                 |                                                                                                                                               
gATGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTctcg                                                                                                                                               <  2:115590/148‑1 (MQ=255)
   ggTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATg                                                                                                                                            <  1:687948/148‑1 (MQ=255)
    gTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGt                                                                                                                                           >  2:3718694/1‑148 (MQ=255)
       aTGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAg                                                                                                                                        >  2:3063562/1‑148 (MQ=255)
         gCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCa                                                                                                                                      <  2:3400699/148‑1 (MQ=255)
               acgCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGcc                                                                                                                                <  1:1491978/148‑1 (MQ=255)
                  cACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGa                                                                                                                             >  2:2291657/1‑148 (MQ=255)
                  cACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGa                                                                                                                             <  1:3581058/148‑1 (MQ=255)
                  cACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGa                                                                                                                             <  1:1754513/148‑1 (MQ=255)
                      tCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCa                                                                                                                         >  2:1061488/1‑148 (MQ=255)
                       cGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAg                                                                                                                        >  2:2141534/1‑148 (MQ=255)
                          tgttACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAgctg                                                                                                                     <  1:1196131/148‑1 (MQ=255)
                             tACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTc                                                                                                                  >  2:1467701/1‑148 (MQ=255)
                             tACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTc                                                                                                                  >  2:1617790/1‑148 (MQ=255)
                             tACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTc                                                                                                                  >  2:3368518/1‑148 (MQ=255)
                             tACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTc                                                                                                                  >  2:1838065/1‑148 (MQ=255)
                              aCGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCa                                                                                                                 <  2:3027748/148‑1 (MQ=255)
                               cGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCAt                                                                                                                >  1:1220017/1‑148 (MQ=255)
                                   cTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGga                                                                                                            >  1:984193/1‑148 (MQ=255)
                                    ttCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGTTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGgag                                                                                                           >  2:2049549/1‑148 (MQ=255)
                                    ttCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGgag                                                                                                           <  1:2726572/148‑1 (MQ=255)
                                    ttCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGgag                                                                                                           <  1:37949/148‑1 (MQ=255)
                                    ttCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGgag                                                                                                           <  1:811316/148‑1 (MQ=255)
                                     tCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGgaga                                                                                                          <  2:2037646/148‑1 (MQ=255)
                                        gagaTAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGAttt                                                                                                       >  2:2965677/1‑148 (MQ=255)
                                         agaTAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGAtttt                                                                                                      >  1:2371548/1‑148 (MQ=255)
                                         agaTAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGAtttt                                                                                                      <  2:1042805/148‑1 (MQ=255)
                                          gaTAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTc                                                                                                     <  1:2547688/148‑1 (MQ=255)
                                             aaTCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGTCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACt                                                                                                  >  2:1295688/1‑148 (MQ=255)
                                             aaTCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACt                                                                                                  >  2:3564172/1‑148 (MQ=255)
                                               tcatcTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTcc                                                                                                <  2:576969/148‑1 (MQ=255)
                                               tcatcTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTcc                                                                                                <  1:547842/148‑1 (MQ=255)
                                               tcatcTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTcc                                                                                                <  2:3324004/148‑1 (MQ=255)
                                                  tctcTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCAt                                                                                             >  2:2032399/1‑148 (MQ=255)
                                                   ctctTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCAtt                                                                                            >  2:1901085/1‑148 (MQ=255)
                                                       ttGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCt                                                                                        >  2:2776155/1‑148 (MQ=255)
                                                       ttGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCt                                                                                        >  2:746225/1‑148 (MQ=255)
                                                           ggATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGccc                                                                                    <  1:2458500/148‑1 (MQ=255)
                                                             aTCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCa                                                                                  <  2:1163588/148‑1 (MQ=255)
                                                              tCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCAt                                                                                 <  1:595814/148‑1 (MQ=255)
                                                              tCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCAt                                                                                 >  2:2692424/1‑148 (MQ=255)
                                                               cTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATc                                                                                >  2:2200321/1‑148 (MQ=255)
                                                                   ttACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACCGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACACCCATTATCTGCCCCATCTGCa                                                                            >  2:2526010/1‑148 (MQ=255)
                                                                     aCGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAgg                                                                          >  1:1731113/1‑148 (MQ=255)
                                                                         tAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGgatga                                                                      <  1:1331932/148‑1 (MQ=255)
                                                                         tAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGgatga                                                                      <  1:3688451/148‑1 (MQ=255)
                                                                          aGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGgatgat                                                                     <  1:2651280/148‑1 (MQ=255)
                                                                           ggTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCGGCCCCATCTGCAGGATGAtt                                                                    <  2:2683104/148‑1 (MQ=255)
                                                                               ttgtAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGAt                                                                <  1:921506/148‑1 (MQ=255)
                                                                               ttgtAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGAt                                                                <  1:618290/148‑1 (MQ=255)
                                                                                tgtAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATc                                                               <  1:2785372/148‑1 (MQ=255)
                                                                                 gtAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATcc                                                              <  2:1391515/148‑1 (MQ=255)
                                                                                  tAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCa                                                             >  2:1566156/1‑148 (MQ=255)
                                                                                  tAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCa                                                             >  2:877692/1‑148 (MQ=255)
                                                                                  tAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCa                                                             >  2:877744/1‑148 (MQ=255)
                                                                                  tAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCa                                                             >  1:3626687/1‑148 (MQ=255)
                                                                                  tAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCa                                                             <  1:805217/148‑1 (MQ=255)
                                                                                  tAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCa                                                             <  1:805026/148‑1 (MQ=255)
                                                                                     ttCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCAATGa                                                          >  1:408699/1‑148 (MQ=255)
                                                                                          tCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCaaa                                                     >  2:246335/1‑148 (MQ=255)
                                                                                          tCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCaaa                                                     <  1:3063562/148‑1 (MQ=255)
                                                                                           ccATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAAc                                                    >  1:3277550/1‑148 (MQ=255)
                                                                                           ccATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAAc                                                    >  2:3580598/1‑148 (MQ=255)
                                                                                            cATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACg                                                   <  2:2536119/148‑1 (MQ=255)
                                                                                             aTCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGt                                                  <  2:2000267/148‑1 (MQ=255)
                                                                                               cGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTc                                                >  2:2776127/1‑148 (MQ=255)
                                                                                                 tAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCa                                              >  1:2537048/1‑148 (MQ=255)
                                                                                                 tAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCa                                              <  2:660609/148‑1 (MQ=255)
                                                                                                 tAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCa                                              >  2:2973729/1‑148 (MQ=255)
                                                                                                    gCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGa                                           >  2:550268/1‑148 (MQ=255)
                                                                                                    gCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGa                                           <  1:2291657/148‑1 (MQ=255)
                                                                                                    gCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGa                                           <  2:2533565/148‑1 (MQ=255)
                                                                                                     ccAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGAt                                          >  1:3612326/1‑148 (MQ=255)
                                                                                                        gACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTtg                                       >  1:1749962/1‑148 (MQ=255)
                                                                                                         aCGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTtgg                                      <  2:1300818/148‑1 (MQ=255)
                                                                                                         aCGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTtgg                                      >  2:3182471/1‑148 (MQ=255)
                                                                                                         aCGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTtgg                                      <  2:763454/148‑1 (MQ=255)
                                                                                                         aCGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTtgg                                      <  1:1110453/148‑1 (MQ=255)
                                                                                                          cGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTtggt                                     <  1:442390/148‑1 (MQ=255)
                                                                                                           ggCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTtggtg                                    <  1:387489/148‑1 (MQ=255)
                                                                                                               gcgcATCTGATCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGgcgc                                >  1:447736/1‑148 (MQ=255)
                                                                                                                         tCCAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAgcgtcgcgt                      >  2:110821/1‑148 (MQ=255)
                                                                                                                           cAGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGtca                    <  1:3330061/148‑1 (MQ=255)
                                                                                                                            aGCGTCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGtcat                   >  1:2068145/1‑148 (MQ=255)
                                                                                                                                tCATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAgg               >  2:2063079/1‑148 (MQ=255)
                                                                                                                                 cATGTTGTTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGa              >  1:3373793/1‑148 (MQ=255)
                                                                                                                                   tgttgtTCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAg            <  1:1061488/148‑1 (MQ=255)
                                                                                                                                    gttgttCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGt           <  1:1266702/148‑1 (MQ=255)
                                                                                                                                    gttgttCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGt           >  2:1525379/1‑148 (MQ=255)
                                                                                                                                     ttgttCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGtt          >  1:772745/1‑148 (MQ=255)
                                                                                                                                      tgttCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTtg         >  1:661652/1‑148 (MQ=255)
                                                                                                                                        ttCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTtgct       <  1:1838065/148‑1 (MQ=255)
                                                                                                                                        ttCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTtgct       >  2:170987/1‑148 (MQ=255)
                                                                                                                                         tCTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTtgctg      >  2:3257637/1‑148 (MQ=255)
                                                                                                                                          cTGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTtgctgc     <  1:536687/148‑1 (MQ=255)
                                                                                                                                           tGTTTCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCtgcc    <  1:273559/148‑1 (MQ=255)
                                                                                                                                           tGTTTCTCGATGTTAGAAATCGCCTGATCCAGCTGCTCATCGGATATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTAATCAGGAAGTTGCtgcc    <  2:275888/148‑1 (MQ=255)
                                                                                                                                             tttCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCtgcctt  >  1:2850597/1‑148 (MQ=255)
                                                                                                                                             tttCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCtgcctt  >  1:3438925/1‑148 (MQ=255)
                                                                                                                                             tttCTCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCtgcctt  >  1:937932/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GATGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTCCATCGTAAGCCAGACGGCTGCGCATCTGATCCAGCGTCATGTTGTTCTGTTTCGCGATGTTAGCAATCGCCTGATCCAGCTGCTCATCGGAGATTTTCACTCCCATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTT  >  CP009273/54247‑54535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑