Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 486,494 C→A 100% R53S (CGC→AGC)  ybaN → DUF454 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273486,4940CA98.7% 757.5 / 0.6 234R53S (CGC→AGC) ybaNDUF454 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base A (121/110);  total (122/112)
Fisher's exact test for biased strand distribution p-value = 6.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 234 total aligned reads displayed.

CATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACCGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGATTTTGCTTATTTTGCTCACGTTTGCCATTTCTCTGTGGTTCGTCCAGATGCCATGGGTGCGCATCATGT  >  CP009273/486349‑486638
                                                                                                                                                 |                                                                                                                                                
cATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGc                                                                                                                                                >  1:828412/1‑148 (MQ=255)
   tttAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCa                                                                                                                                             >  2:3536343/1‑148 (MQ=255)
   tttAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCa                                                                                                                                             >  2:1820325/1‑148 (MQ=255)
   tttAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCa                                                                                                                                             >  2:2468253/1‑148 (MQ=255)
        tcatcaTTGGCTGGCTGGCGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGtt                                                                                                                                        >  2:2793162/1‑148 (MQ=255)
        tcatcaTTGGCTGGCTGGCGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGtt                                                                                                                                        <  2:3400643/148‑1 (MQ=255)
        tcatcaTTGGCTGGCTGGCGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGtt                                                                                                                                        >  2:3709564/1‑148 (MQ=255)
        tcatcaTTGGCTGGCTGGCGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGtt                                                                                                                                        >  1:77904/1‑148 (MQ=255)
        tcatcaTTGGCTGGCTGGCGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGtt                                                                                                                                        >  2:3070789/1‑148 (MQ=255)
        tcatcaTTGGCTGGCTGGCGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGtt                                                                                                                                        <  2:1080038/148‑1 (MQ=255)
        tcatcaTTGGCTGGCTGGCGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGtt                                                                                                                                        >  1:2931098/1‑148 (MQ=255)
         catcatTGGCTGGCTGGCGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGttt                                                                                                                                       >  1:3320873/1‑148 (MQ=255)
         catcatTGGCTGGCTGGCGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGttt                                                                                                                                       >  2:3083425/1‑148 (MQ=255)
         catcatTGGCTGGCTGGCGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGttt                                                                                                                                       >  1:1416746/1‑148 (MQ=255)
         catcatTGGCTGGCTGGCGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGttt                                                                                                                                       >  1:1315944/1‑148 (MQ=255)
         catcatTGGCTGGCTGGCGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGttt                                                                                                                                       >  1:976263/1‑148 (MQ=255)
          atcatTGGCTGGCTGGCGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTg                                                                                                                                      >  1:1967665/1‑148 (MQ=255)
            catTGGCTGGCTGGCGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGc                                                                                                                                    <  2:38286/148‑1 (MQ=255)
             atTGGCTGGCTGGCGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCa                                                                                                                                   >  2:2894851/1‑148 (MQ=255)
              ttGGCTGGCTGGCGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAg                                                                                                                                  >  2:773367/1‑148 (MQ=255)
                ggctggctggcGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCt                                                                                                                                <  1:3409702/148‑1 (MQ=255)
                 gctggctggcGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTa                                                                                                                               >  1:3359542/1‑148 (MQ=255)
                 gctggctggcGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTa                                                                                                                               >  1:2638104/1‑148 (MQ=255)
                 gctggctggcGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTa                                                                                                                               >  1:951354/1‑148 (MQ=255)
                 gctggctggcGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTa                                                                                                                               <  1:1917397/148‑1 (MQ=255)
                  ctggctggcGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTAt                                                                                                                              <  1:1228256/148‑1 (MQ=255)
                  ctggctggcGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTAt                                                                                                                              <  1:3615008/148‑1 (MQ=255)
                    ggctggcGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCt                                                                                                                            <  1:1184721/148‑1 (MQ=255)
                       tggcGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACg                                                                                                                         <  1:3619403/148‑1 (MQ=255)
                        ggcGGTAGTGCTGGTTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGt                                                                                                                        <  1:2264672/148‑1 (MQ=255)
                         gcGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGtt                                                                                                                       <  2:3413670/148‑1 (MQ=255)
                          cGGTAGTGCTGGGTTCGCTGGGCGTGGTATTAACGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTTCTTTGCCCGTTCTTCCCCGAGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGttt                                                                                                                      <  1:1471783/148‑1 (MQ=255)
                          cGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGttt                                                                                                                      <  1:502432/148‑1 (MQ=255)
                          cGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGttt                                                                                                                      >  2:2351074/1‑148 (MQ=255)
                            gTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCt                                                                                                                    >  1:2171850/1‑148 (MQ=255)
                             tAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTg                                                                                                                   >  1:1643772/1‑148 (MQ=255)
                                   tGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGaa                                                                                                             <  1:2186551/148‑1 (MQ=255)
                                     ggTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAAc                                                                                                           >  2:1956885/1‑148 (MQ=255)
                                     ggTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAAc                                                                                                           <  2:3249496/148‑1 (MQ=255)
                                      gTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAAca                                                                                                          <  2:3126397/148‑1 (MQ=255)
                                       tACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAAcat                                                                                                         <  2:1603621/148‑1 (MQ=255)
                                       tACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAAcat                                                                                                         <  1:483076/148‑1 (MQ=255)
                                         cGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAAcatca                                                                                                       <  1:1418088/148‑1 (MQ=255)
                                             gggCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCg                                                                                                   >  1:2858425/1‑148 (MQ=255)
                                             gggCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCg                                                                                                   >  2:659489/1‑148 (MQ=255)
                                             gggCGTGGTATTACCGGTATGACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGCTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCg                                                                                                   <  1:3537021/148‑1 (MQ=255)
                                              ggCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGa                                                                                                  >  2:332366/1‑148 (MQ=255)
                                               gCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGAt                                                                                                 >  1:3813606/1‑148 (MQ=255)
                                                     tattaccggtattaccgACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCcgcg                                                                                           >  2:1772459/1‑148 (MQ=255)
                                                            ggtattaccgACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTc                                                                                    <  2:608564/148‑1 (MQ=255)
                                                              tattaccgACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCaa                                                                                  <  1:3132680/148‑1 (MQ=255)
                                                                ttaccgACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAAc                                                                                >  2:1009662/1‑148 (MQ=255)
                                                                 taccgACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAAcc                                                                               >  2:2770232/1‑148 (MQ=255)
                                                                   ccgACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACcgc                                                                             <  2:3285765/148‑1 (MQ=255)
                                                                         acgCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGa                                                                       <  1:1120122/148‑1 (MQ=255)
                                                                         acgCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGa                                                                       <  1:1119188/148‑1 (MQ=255)
                                                                            ccGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGAttt                                                                    >  2:601454/1‑148 (MQ=255)
                                                                            ccGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGAttt                                                                    >  2:1751799/1‑148 (MQ=255)
                                                                              gTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGATTTTg                                                                  >  2:2172011/1‑148 (MQ=255)
                                                                               tttATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGATTTTGc                                                                 >  2:1650675/1‑148 (MQ=255)
                                                                               tttATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGATTTTGc                                                                 <  2:1219433/148‑1 (MQ=255)
                                                                                ttATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGATTTTGCt                                                                >  1:785316/1‑148 (MQ=255)
                                                                                ttATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGATTTTGCt                                                                >  1:3507795/1‑148 (MQ=255)
                                                                                  aTCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGAGTTTGCGTa                                                              >  2:2962222/1‑148 (MQ=255)
                                                                                   tCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGATTTTGCTTAt                                                             <  1:259748/148‑1 (MQ=255)
                                                                                   tCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGATTTTGCTTAt                                                             >  2:2817799/1‑148 (MQ=255)
                                                                                       gctgGCGGCCTGGTGCTTTGCCCGTTCTTCCCCTCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGATTTTGCTTATTTTg                                                         <  1:2804862/148‑1 (MQ=255)
                                                                                       gctgGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGATTTTGCTTATTTTg                                                         <  1:2105539/148‑1 (MQ=255)
                                                                                         ttgcgggCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGAGGCGTCAAACCGCGTGCTATTTTGATTATTTTGCt                                                       <  2:45672/146‑1 (MQ=255)
                                                                                            cggcCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGATTTTGCTTATTTTGCTCAc                                                    >  2:1975323/1‑148 (MQ=255)
                                                                                                   gTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGATTTTGCTTATTTTGCTCACGTTTGcc                                             <  1:2468253/148‑1 (MQ=255)
                                                                                                       tttGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGATTTTGCTTATTTTGCTCACGTTTGCCAttt                                         <  2:862313/148‑1 (MQ=255)
                                                                                                        ttGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGATTTTGCTTATTTTGCTCACGTTTGCCATTtc                                        >  2:1161196/1‑148 (MQ=255)
                                                                                                         tGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGAGTTTGCTTATTTTGCTCACGTTTGCCATTtct                                       >  1:3772229/1‑148 (MQ=255)
                                                                                                           cccGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGATTTTGCTTATTTTGCTCACGTTTGCCATTTctct                                     <  2:940360/148‑1 (MQ=255)
                                                                                                           cccGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGATTTTGCTTATTTTGCTCACGTTTGCCATTTctct                                     <  2:1551212/148‑1 (MQ=255)
                                                                                                           cccGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGATTTTGCTTATTTTGCTCACGTTTGCCATTTctct                                     >  2:1481147/1‑148 (MQ=255)
                                                                                                           cccGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGATTTTGCTTATTTTGCTCACGTTTGCCATTTctct                                     >  2:3382757/1‑148 (MQ=255)
                                                                                                            ccGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGATTTTGCTTATTTTGCTCACGTTTGCCATTTCTCtg                                    <  2:3652371/148‑1 (MQ=255)
                                                                                                            ccGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGATTTTGCTTATTTTGCTCACGTTTGCCATTTCTCtg                                    <  2:904458/148‑1 (MQ=255)
                                                                                                            ccGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGATTTTGCTTATTTTGCTCACGTTTGCCATTTCTCtg                                    <  2:2583044/148‑1 (MQ=255)
                                                                                                             cGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGATTTTGCTTATTTTGCTCACGTTTGCCATTTCTCtgt                                   >  1:901086/1‑148 (MQ=255)
                                                                                                                    ccccGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGATTTTGCTTATTTTGCTCACGTTTGCCATTTCTCTGTGGTTAGt                            >  1:657629/1‑148 (MQ=255)
                                                                                                                     cccGCGCTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGATTTTGCTTATTTTGCTCACGTTTGCCATTTCTCTGTGGTTCGTc                           <  2:2931098/148‑1 (MQ=255)
                                                                                                                        gcgcTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGTCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGATTTTGCTTATTTTGCTCACGTTTGCCATTTCTCTGTGGTTCGTCCAg                        <  2:3761574/148‑1 (MQ=255)
                                                                                                                        gcgcTTTCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGATTTTGCTTATTTTGCTCACGTTTGCCATTTCTCTGTGGTTCGTCCAg                        <  2:3761627/148‑1 (MQ=255)
                                                                                                                            tttCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGATTTTGCTTATTTTGCTCACGTTTGCCATTTCTCTGTGGTTCGTCCAGATGc                    <  1:2944116/148‑1 (MQ=255)
                                                                                                                            tttCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGATTTTGCTTATTTTGCTCACGTTTGCCATTTCTCTGTGGTTCGTCCAGATGc                    <  1:3070696/148‑1 (MQ=255)
                                                                                                                            tttCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGATTTTGCTTATTTTGCTCACGTTTGCCATTTCTCTGTGGTTCGTCCAGATGc                    <  2:2858425/148‑1 (MQ=255)
                                                                                                                            tttCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGATTTTGCTTATTTTGCTCACGTTTGCCATTTCTCTGTGGTTCGTCCAGATGc                    >  1:3177301/1‑148 (MQ=255)
                                                                                                                            tttCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGATTTTGCTTATTTTGCTCACGTTTGCCATTTCTCTGTGGTTCGTCCAGATGc                    >  2:3016128/1‑148 (MQ=255)
                                                                                                                             ttCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGATTTTGCTTATTTTGCTCACGTTTGCCATTTCTCTGTGGTTCGTCCAGATGcc                   >  1:447992/1‑148 (MQ=255)
                                                                                                                             ttCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGATTTTGCTTATTTTGCTCACGTTTGCCATTTCTCTGTGGTTCGTCCAGATGcc                   >  2:2872602/1‑148 (MQ=255)
                                                                                                                             ttCACGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGATTTTGCTTATTTTGCTCACGTTTGCCATTTCTCTGTGGTTCGTCCAGATGcc                   <  1:1840463/148‑1 (MQ=255)
                                                                                                                             ttCACGCCTGGTTGCTGTACAGCTCATGGCTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGATTTTGCTTATTTTGCTCACGTTTGCCATTTCTCTGTGGTTCGTCCAGATGcc                   <  2:1919120/148‑1 (MQ=255)
                                                                                                                                aCGCCTGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGATTTTGCTTATTTTGCTCACGTTTGCCATTTCTCTGTGGTTCGTCCAGATGCCATg                <  2:2566280/148‑1 (MQ=255)
                                                                                                                                     tGGTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGATTTTGCTTATTTTGCTCACGTTTGCCATTTCTCTGTGGTTCGTCCAGATGCCATGGGTgc           >  1:1286550/1‑148 (MQ=255)
                                                                                                                                      ggTTGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGATTTTGCTTATTTTGCTCACGTTTGCCATTTCTCTGTGGTTCGTCCAGATGCCATGGGTgcg          >  1:2825523/1‑148 (MQ=255)
                                                                                                                                        ttGCTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGATTTTGCTTATTTTTCTAAAGTTTTCAATTTATATTTGTTTAGTCCACATTCCATTGGTGCGca        >  1:1618076/1‑148 (MQ=255)
                                                                                                                                           cTGTACAGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGATTTTGCTTATTTTGCTCACGTTTGCCATTTCTCTGTGGTTCGTCCAGATGCCATGGGTGCGcatca     >  1:950679/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACCGCTCATGGTTTGGCAGCTATCTACGTTTCTGGCAGAAACATCATGCGATGCCGCGCGGCGTCAAACCGCGGGCGATTTTGCTTATTTTGCTCACGTTTGCCATTTCTCTGTGGTTCGTCCAGATGCCATGGGTGCGCATCATGT  >  CP009273/486349‑486638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑