Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 503,944 G→T 100% E91* (GAA→TAA)  ybaQ → putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273503,9440GT100.0% 618.4 / ‑6.7 223E91* (GAA→TAA) ybaQputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (3/2);  major base T (89/121);  minor base A (8/0);  total (100/123)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 233 total aligned reads displayed.

TGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCTG  >  CP009273/503799‑504090
                                                                                                                                                 |                                                                                                                                                  
tGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTTGCGAAAACTCCAGGCGGCGGTTGTTCTTTTGGTAGTTGAAAACAAAAAGCGCCCCCCGgaagaa                                                                                                                                                  >  2:1623021/1‑148 (MQ=255)
tGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGgaataa                                                                                                                                                  >  2:3016364/1‑148 (MQ=255)
   aCTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGACTAATTg                                                                                                                                               >  2:2032805/1‑148 (MQ=255)
   aCTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAAttt                                                                                                                                               >  2:2033066/1‑147 (MQ=255)
   aCTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTg                                                                                                                                               <  2:721715/148‑1 (MQ=255)
   aCTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTg                                                                                                                                               <  1:1257541/148‑1 (MQ=255)
     tGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTggg                                                                                                                                             <  1:329374/148‑1 (MQ=255)
     tGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTggg                                                                                                                                             <  1:329539/148‑1 (MQ=255)
      gATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGTTAGTTGGGAAGTTGAAAACAACATGCGCACCCAGGAAAAATTGGGa                                                                                                                                            >  1:1595574/1‑148 (MQ=255)
        tCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACg                                                                                                                                          <  1:652643/148‑1 (MQ=255)
         cAATAAAAATCGTAAACTCACTACTGAGATGTCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACgg                                                                                                                                         <  1:2002784/148‑1 (MQ=255)
            tAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTTGCTAAAACACCAGGCGGCGGGTGTTCTTTGGGTAGTTGTAAACAAACTGCGCCCCCAGGACTAATTTGGGCGGGtt                                                                                                                                      >  1:3100308/1‑148 (MQ=255)
            tAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGGTAAAACTCCAACCGGCGGGTTGTTATTGGGTACTAGTAAACAACATGCGCACCCAGGAATAAATGGGGCGGGtt                                                                                                                                      >  1:3627239/1‑148 (MQ=255)
               cAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCCGGCATAATTGGGACGGATTGaa                                                                                                                                   >  2:3672110/1‑148 (MQ=255)
               cAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGaa                                                                                                                                   <  1:3763211/148‑1 (MQ=255)
               cAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGaa                                                                                                                                   <  2:3407052/148‑1 (MQ=255)
                  tCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACa                                                                                                                                <  1:3211137/148‑1 (MQ=255)
                   cGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAg                                                                                                                               <  1:797075/148‑1 (MQ=255)
                   cGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAg                                                                                                                               <  1:1427751/148‑1 (MQ=255)
                   cGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAg                                                                                                                               <  2:2854396/148‑1 (MQ=255)
                   cGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAAAATGCGCACCCAGGAATAATTTGGACGGATTGAAACAg                                                                                                                               >  2:1168881/1‑148 (MQ=255)
                     tAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTTGCTAAACAACCAGGCGGCGGGTGATATTTTGGGATTTTAAAAAAAAAAACGCACCCAGGCAAAAATGGGACGGAATGaaaaaaag                                                                                                                             >  1:844852/1‑143 (MQ=255)
                     tAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGAGTTTTGGGTAAACCACCAGGCGGCGGGTGATCGTTGGGGACTTGTAAACAACAAGGGCACCCCCGAATAATTGGGCCGGTTTGAAAAAggg                                                                                                                             >  1:2758761/1‑146 (MQ=255)
                      aaaCTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTgg                                                                                                                            <  2:1896920/148‑1 (MQ=255)
                        aCTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAAAAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCt                                                                                                                          >  2:2545263/1‑148 (MQ=255)
                          tCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGa                                                                                                                        <  1:636468/148‑1 (MQ=255)
                          tCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGa                                                                                                                        <  1:636414/148‑1 (MQ=255)
                            actactGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTTGCTAAAACAACAGGCGGGGGGTGTTGTTTTGGTAGTTTTAAACAAAAAGCGCACCCCCGAATAATTTGGACGGATTGTAAAACTGGCTGAat                                                                                                                      >  1:3009785/1‑148 (MQ=255)
                             ctactGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAata                                                                                                                     <  2:3001732/148‑1 (MQ=255)
                             ctactGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAata                                                                                                                     <  1:1259287/148‑1 (MQ=255)
                             ctactGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAata                                                                                                                     <  1:120362/148‑1 (MQ=255)
                             ctactGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAata                                                                                                                     <  1:100357/148‑1 (MQ=255)
                             ctactGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCACCAGGCGGCGGGTGTTCTTTGGGAAGTTGAAAACAACATTCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCGGtata                                                                                                                     >  2:2985982/1‑148 (MQ=255)
                              tactGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTTGCTAAAACTCCAGGCGGCGGGTGATCTTTGGGTAGTTGTAAACAAAATGCGCACCCAGGCAAAATTGGGACGGCTTGAAACAGTGGCTGAatat                                                                                                                    >  2:1404009/1‑148 (MQ=255)
                              tactGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCACCAGGCGGCGGTTGATCTTTGGGAACTTGAAAACAACATGCGCCCCCAGGAATAATTTGGCCGGATTGAAACACTGGCTGAatat                                                                                                                    >  2:3308085/1‑148 (MQ=255)
                              tactGAGATGGAATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAatat                                                                                                                    <  2:2723535/148‑1 (MQ=255)
                                   agaTGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTTGCTAAACCTCCAGGCGGCGGTTGTTGGTTGGGGAGTTGTAAACAACCAGCGCCCCCCGGAATAAGTGGGGCGGATTGTAACAGTGGCGGTATATTTGGc                                                                                                               >  1:3812105/1‑148 (MQ=255)
                                        gCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGGTAAACCTCCAGGCGGCGGTTGATCGTTGGGCAGTTGAAAACCACATGCGCACCCAGGAATAATTGGGCCGGATTGTAACAGTGGCGGAATATTTGGCACGcc                                                                                                          >  2:3057685/1‑148 (MQ=255)
                                         cATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCg                                                                                                         <  1:2541086/148‑1 (MQ=255)
                                          aTTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGt                                                                                                        <  1:3599842/148‑1 (MQ=255)
                                           tttCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGGTGATCTTTGGGAAGTTGTAAACAACAAGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGTATATTTTGCAAGCCGTg                                                                                                       >  2:1060483/1‑148 (MQ=255)
                                            ttCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTTGCTAAACCTCCAGGCGGCGGTTGTTCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACACTGGCTGAAAATTTGGCACGCCGTGa                                                                                                      >  2:3778098/1‑148 (MQ=255)
                                            ttCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGGTGGTCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAAAAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGa                                                                                                      >  2:278538/1‑148 (MQ=255)
                                              cGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAag                                                                                                    <  1:3495161/148‑1 (MQ=255)
                                               gTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAaga                                                                                                   <  1:1844960/148‑1 (MQ=255)
                                                         aGTTTTTGATACCACAGTCGATTTTTTGCGAAAACACCCGGCCGCGGGTGTTCGTTGGGAAATTGAAAACAAAATGCGCACCCCGGAATAAATTGGGCGGATTTAAACACTGGCTGTAAATTTGGCACGCCGGGAACTGGGGGCaaaa                                                                                         >  2:1360069/1‑148 (MQ=255)
                                                         aGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCaaaa                                                                                         <  2:2460193/148‑1 (MQ=255)
                                                          gTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCaaaaa                                                                                        <  2:1616468/148‑1 (MQ=255)
                                                              ttGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGt                                                                                    <  2:142252/148‑1 (MQ=255)
                                                              ttGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGt                                                                                    <  2:1293128/148‑1 (MQ=255)
                                                                   aCCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGt                                                                               <  2:3154588/148‑1 (MQ=255)
                                                                   aCCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGt                                                                               <  2:1035450/148‑1 (MQ=255)
                                                                    ccACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTa                                                                              <  1:2936508/148‑1 (MQ=255)
                                                                       caGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAgg                                                                           <  2:2178600/148‑1 (MQ=255)
                                                                        aGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGc                                                                          <  1:4693/148‑1 (MQ=255)
                                                                         gTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATATTTGGGAAGTTGAAAACAACATGCGCACCCAGGCAAAATTGGGACGGATTTAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTTAGGcc                                                                         >  1:1083340/1‑148 (MQ=255)
                                                                          tCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCa                                                                        >  2:1922966/1‑148 (MQ=255)
                                                                          tCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCa                                                                        >  1:2478157/1‑148 (MQ=255)
                                                                          tCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCa                                                                        <  1:3255835/148‑1 (MQ=255)
                                                                          tCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACACCGTGAAGATCGTGCAAAAAAGGTCGCGTAAGGCCa                                                                        >  1:2681864/1‑148 (MQ=255)
                                                                            gATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCaaa                                                                      <  1:3552451/148‑1 (MQ=255)
                                                                             aTTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCaaaa                                                                     >  1:3504540/1‑148 (MQ=255)
                                                                              tttttGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAAc                                                                    >  2:1542612/1‑148 (MQ=255)
                                                                               ttttGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACa                                                                   <  1:1587454/148‑1 (MQ=255)
                                                                               ttttGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACa                                                                   <  1:3485391/148‑1 (MQ=255)
                                                                                 ttGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATc                                                                 <  1:3188736/148‑1 (MQ=255)
                                                                                     cTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCtt                                                             <  1:1354745/148‑1 (MQ=255)
                                                                                      tAAACCTCCAGGCGGCGTTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAATGGTCGCGTAAGGCCAAAACATCGCTTc                                                            <  2:1588827/148‑1 (MQ=255)
                                                                                       aaaCCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCa                                                           >  1:3167872/1‑148 (MQ=255)
                                                                                       aaaCCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCa                                                           <  2:3388796/148‑1 (MQ=255)
                                                                                       aaaCCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCa                                                           <  1:149591/148‑1 (MQ=255)
                                                                                          ccTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTTGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCAtct                                                        >  1:998932/1‑148 (MQ=255)
                                                                                            tCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCg                                                      <  1:2962174/148‑1 (MQ=255)
                                                                                            tCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCg                                                      <  1:3051133/148‑1 (MQ=255)
                                                                                               aGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAg                                                   >  2:3576685/1‑148 (MQ=255)
                                                                                                 gcggcgGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGc                                                 >  1:1187889/1‑148 (MQ=255)
                                                                                                 gcggcgGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGc                                                 >  2:3502626/1‑148 (MQ=255)
                                                                                                        ttGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATaa                                          <  2:1873469/148‑1 (MQ=255)
                                                                                                        ttGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATaa                                          <  2:2232668/148‑1 (MQ=255)
                                                                                                        ttGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATaa                                          >  2:635766/1‑148 (MQ=255)
                                                                                                        ttGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATaa                                          >  2:3424440/1‑148 (MQ=255)
                                                                                                            tCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAATAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCg                                      >  2:2203276/1‑148 (MQ=255)
                                                                                                                  ggAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCAcg                                >  1:3719191/1‑148 (MQ=255)
                                                                                                                   gAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCAcgc                               >  1:1032931/1‑148 (MQ=255)
                                                                                                                       ttGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgca                           <  1:583940/148‑1 (MQ=255)
                                                                                                                             acaacaTGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccggc                     >  2:2969344/1‑148 (MQ=255)
                                                                                                                             acaacaTGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccggc                     <  1:3405593/148‑1 (MQ=255)
                                                                                                                              caacaTGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccggca                    >  1:2321560/1‑148 (MQ=255)
                                                                                                                               aacaTGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccggcat                   <  1:2645234/148‑1 (MQ=255)
                                                                                                                               aacaTGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccggcat                   <  2:215793/148‑1 (MQ=255)
                                                                                                                                acaTGCGCACCCAGGAAGACTTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccggcatc                  <  2:1720494/148‑1 (MQ=255)
                                                                                                                                  aTGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccggcatccg                <  2:2343573/148‑1 (MQ=255)
                                                                                                                                   tGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGa               >  1:701169/1‑148 (MQ=255)
                                                                                                                                   tGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGa               <  1:1859920/148‑1 (MQ=255)
                                                                                                                                   tGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGa               <  1:1404009/148‑1 (MQ=255)
                                                                                                                                         cccAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCa         >  2:295046/1‑148 (MQ=255)
                                                                                                                                            aGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTg      <  2:542022/148‑1 (MQ=255)
                                                                                                                                              gaataaTTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGTATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGcc    >  1:278412/1‑148 (MQ=255)
                                                                                                                                              gaataaTTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACGATTGcg    >  2:3531073/1‑147 (MQ=255)
                                                                                                                                                agaaTTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCTg  <  2:850406/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
TGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCTG  >  CP009273/503799‑504090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑