Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 549,397 G→T 100% intergenic (‑1/+2) lpxH ← / ← ppiB UDP‑2,3‑diacylglucosamine pyrophosphohydrolase/peptidyl‑prolyl cis‑trans isomerase B (rotamase B)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273549,3970GT100.0% 774.4 / ‑6.7 236intergenic (‑1/+2)lpxH/ppiBUDP‑2,3‑diacylglucosamine pyrophosphohydrolase/peptidyl‑prolyl cis‑trans isomerase B (rotamase B)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (128/107);  minor base A (1/0);  total (129/107)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 236 total aligned reads displayed.

GCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACGATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCC  >  CP009273/549253‑549540
                                                                                                                                                |                                                                                                                                               
gCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTAtt                                                                                                                                              <  1:2459641/148‑1 (MQ=255)
 ccAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTa                                                                                                                                             >  2:87148/1‑148 (MQ=255)
  cAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTAc                                                                                                                                            >  1:100619/1‑148 (MQ=255)
  cAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTAc                                                                                                                                            <  1:3814398/148‑1 (MQ=255)
   aaTCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACt                                                                                                                                           <  1:288612/148‑1 (MQ=255)
     tCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCg                                                                                                                                         >  2:2791518/1‑148 (MQ=255)
      ccATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGc                                                                                                                                        <  2:2657872/148‑1 (MQ=255)
      ccATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGc                                                                                                                                        >  1:2824181/1‑148 (MQ=255)
        aTGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTa                                                                                                                                      <  2:3074774/148‑1 (MQ=255)
        aTGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTa                                                                                                                                      >  2:1982742/1‑148 (MQ=255)
         tGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTaa                                                                                                                                     <  1:2723587/148‑1 (MQ=255)
         tGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTaa                                                                                                                                     >  1:806210/1‑148 (MQ=255)
          gCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAAc                                                                                                                                    >  1:2032329/1‑148 (MQ=255)
              cAAACAGTTCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGAACAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTc                                                                                                                                <  1:3261260/148‑1 (MQ=255)
               aaaCAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCa                                                                                                                               <  2:852251/148‑1 (MQ=255)
                aaCAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCAc                                                                                                                              >  2:212536/1‑148 (MQ=255)
                  cAGCTCGCCAAGAATATCCCGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGc                                                                                                                            <  2:444849/148‑1 (MQ=255)
                    gATCGCCAATAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCtt                                                                                                                          <  1:444503/148‑1 (MQ=255)
                    gATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCCCTATTACTCGCTAACGGTCACGCtt                                                                                                                          >  2:683397/1‑148 (MQ=255)
                       cGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCa                                                                                                                       <  2:2311116/148‑1 (MQ=255)
                       cGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCa                                                                                                                       <  1:485428/148‑1 (MQ=255)
                        gCCAAGAATATCCAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCaa                                                                                                                      <  1:2972694/148‑1 (MQ=255)
                          cAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATg                                                                                                                    >  1:2520809/1‑148 (MQ=255)
                            aGAATAAACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACCCATAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGAt                                                                                                                  <  1:3520680/148‑1 (MQ=255)
                             gAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATa                                                                                                                 <  1:3119107/148‑1 (MQ=255)
                              aaTATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATaa                                                                                                                <  1:1390734/148‑1 (MQ=255)
                                 ataCAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGt                                                                                                             >  2:649156/1‑148 (MQ=255)
                                  taCAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGtc                                                                                                            <  1:1741954/148‑1 (MQ=255)
                                  taCAGCGCGTCGGCCGTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGtc                                                                                                            <  2:1485503/148‑1 (MQ=255)
                                   aCAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGtct                                                                                                           <  1:931537/148‑1 (MQ=255)
                                    cAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGtctt                                                                                                          <  1:2438475/148‑1 (MQ=255)
                                      gcgcGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGtcttct                                                                                                        <  2:3264198/148‑1 (MQ=255)
                                       cgcgTCGGCCTTGGGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTcttctt                                                                                                       <  2:2485582/148‑1 (MQ=255)
                                       cgcgTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTcttctt                                                                                                       <  2:3804643/148‑1 (MQ=255)
                                       cgcgTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTcttctt                                                                                                       <  2:3804677/148‑1 (MQ=255)
                                                cTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGCGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGt                                                                                              <  1:587279/148‑1 (MQ=255)
                                                     gggCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTgg                                                                                         <  2:382848/148‑1 (MQ=255)
                                                      ggCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGt                                                                                        >  1:1497344/1‑148 (MQ=255)
                                                        cTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGc                                                                                      >  2:1231047/1‑148 (MQ=255)
                                                         ttCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCa                                                                                     >  1:671655/1‑148 (MQ=255)
                                                           cccccGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATa                                                                                   <  1:1406200/148‑1 (MQ=255)
                                                            ccccGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATAc                                                                                  <  2:1558001/148‑1 (MQ=255)
                                                               cGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGc                                                                               >  1:365101/1‑148 (MQ=255)
                                                                gCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCt                                                                              >  1:3357267/1‑148 (MQ=255)
                                                                 cTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTa                                                                             >  2:863813/1‑148 (MQ=255)
                                                                 cTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTa                                                                             >  1:3137783/1‑148 (MQ=255)
                                                                   aaaaaaCGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACg                                                                           >  1:1154985/1‑148 (MQ=255)
                                                                       aaCGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCg                                                                       >  1:2383963/1‑148 (MQ=255)
                                                                        aCGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCgg                                                                      >  2:2277866/1‑148 (MQ=255)
                                                                        aCGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCgg                                                                      >  2:1314751/1‑148 (MQ=255)
                                                                         cGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGt                                                                     >  1:748381/1‑148 (MQ=255)
                                                                         cGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTGTTGGCACGTCCTGGTGCATACCGCTACGACCGGt                                                                     >  1:3405377/1‑148 (MQ=255)
                                                                               aaaCCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAac                                                               <  1:2645646/148‑1 (MQ=255)
                                                                                 aCCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAacac                                                             >  1:334726/1‑148 (MQ=255)
                                                                                  ccGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACAcc                                                            <  2:2872863/148‑1 (MQ=255)
                                                                                   cggcggTGATCGCCGGTTCTTCCACGCAGAGATGAAGTTCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCt                                                           <  2:3700330/148‑1 (MQ=255)
                                                                                   cggcggTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCt                                                           >  1:2777262/1‑148 (MQ=255)
                                                                                   cggcggTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCt                                                           <  1:2791518/148‑1 (MQ=255)
                                                                                       ggTGATCGCCGGTTCTTCCACGCAGAGATGAAGAGCTGCAATCAAGACTGTCGCCACTATGACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGa                                                       <  2:431953/148‑1 (MQ=255)
                                                                                        gTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGAt                                                      >  1:1928475/1‑148 (MQ=255)
                                                                                            tCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTg                                                  >  1:1546965/1‑148 (MQ=255)
                                                                                            tCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTg                                                  >  1:2331974/1‑148 (MQ=255)
                                                                                              gCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTc                                                >  1:3465189/1‑148 (MQ=255)
                                                                                              gCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTc                                                <  2:2455160/148‑1 (MQ=255)
                                                                                               ccGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCt                                               <  1:3715808/148‑1 (MQ=255)
                                                                                                cGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGTTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTa                                              <  2:482535/148‑1 (MQ=255)
                                                                                                cGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTa                                              <  2:1110507/148‑1 (MQ=255)
                                                                                                cGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTa                                              <  2:2173305/148‑1 (MQ=255)
                                                                                                cGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTa                                              <  2:1111464/148‑1 (MQ=255)
                                                                                                 ggTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTAc                                             <  2:3714563/148‑1 (MQ=255)
                                                                                                 ggTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTAc                                             >  2:3727280/1‑148 (MQ=255)
                                                                                                  gTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTAcc                                            >  2:515160/1‑148 (MQ=255)
                                                                                                   ttcttcCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCa                                           <  1:2285777/148‑1 (MQ=255)
                                                                                                   ttcttcCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCa                                           >  2:2826938/1‑148 (MQ=255)
                                                                                                       tcCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTc                                       >  1:834606/1‑148 (MQ=255)
                                                                                                                agaTGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTc                              >  2:598900/1‑148 (MQ=255)
                                                                                                                 gaTGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCa                             <  2:1555122/148‑1 (MQ=255)
                                                                                                                  aTGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCaa                            <  2:2824181/148‑1 (MQ=255)
                                                                                                                     aaGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCa                         >  2:2654163/1‑148 (MQ=255)
                                                                                                                     aaGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCa                         >  2:2759859/1‑148 (MQ=255)
                                                                                                                     aaGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCa                         >  2:2760890/1‑148 (MQ=255)
                                                                                                                      aGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCAc                        <  1:1119200/148‑1 (MQ=255)
                                                                                                                      aGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCAc                        >  1:3085308/1‑148 (MQ=255)
                                                                                                                       gATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACt                       >  1:2613140/1‑148 (MQ=255)
                                                                                                                        aTCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACtt                      >  2:956208/1‑148 (MQ=255)
                                                                                                                        aTCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACtt                      >  2:92289/1‑148 (MQ=255)
                                                                                                                        aTCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACtt                      >  2:2161388/1‑148 (MQ=255)
                                                                                                                          cTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCa                    <  1:1609836/148‑1 (MQ=255)
                                                                                                                               aTAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCaaa               >  2:1142808/1‑148 (MQ=255)
                                                                                                                               aTAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCaaa               >  2:790255/1‑148 (MQ=255)
                                                                                                                                tAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAac              <  1:3028451/148‑1 (MQ=255)
                                                                                                                                tAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAac              <  1:1122908/148‑1 (MQ=255)
                                                                                                                                 aaaGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAaca             <  2:1718708/148‑1 (MQ=255)
                                                                                                                                 aaaGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAaca             >  2:1298455/1‑148 (MQ=255)
                                                                                                                                 aaaGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAaca             <  2:2636817/148‑1 (MQ=255)
                                                                                                                                   agagTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACg           >  1:65653/1‑148 (MQ=255)
                                                                                                                                     agTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCa         >  1:988945/1‑148 (MQ=255)
                                                                                                                                     agTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCa         >  2:2230088/1‑148 (MQ=255)
                                                                                                                                         tCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAg     >  2:2618588/1‑148 (MQ=255)
                                                                                                                                           gCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGcc   >  1:2516930/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                               
GCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACGATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCC  >  CP009273/549253‑549540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑