Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 627,428 C→A 100% I693I (ATC→ATA)  cstA → carbon starvation protein involved in peptide utilization; APC peptide transporter family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273627,4280CA98.6% 682.1 / ‑0.5 214I693I (ATC→ATA) cstAcarbon starvation protein involved in peptide utilization; APC peptide transporter family protein
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base A (131/80);  total (133/81)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 214 total aligned reads displayed.

TGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGT  >  CP009273/627284‑627572
                                                                                                                                                |                                                                                                                                                
tGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTg                                                                                                                                               <  2:3216499/148‑1 (MQ=255)
 gCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTgg                                                                                                                                              <  2:2964062/148‑1 (MQ=255)
     ggTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCa                                                                                                                                          >  1:126592/1‑148 (MQ=255)
        tAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGc                                                                                                                                       >  2:3698153/1‑148 (MQ=255)
        tAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGc                                                                                                                                       >  1:397259/1‑148 (MQ=255)
        tAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGc                                                                                                                                       >  1:396259/1‑148 (MQ=255)
            cATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCaaaa                                                                                                                                   >  1:1791468/1‑148 (MQ=255)
             aTCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAg                                                                                                                                  >  2:2537960/1‑148 (MQ=255)
              tcttctTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAgg                                                                                                                                 >  2:251280/1‑148 (MQ=255)
               cttcttTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGgc                                                                                                                                >  2:3082516/1‑148 (MQ=255)
                 tcttTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGgcgc                                                                                                                              >  1:175252/1‑148 (MQ=255)
                        ggtggtCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGACGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTaa                                                                                                                       <  1:2690120/148‑1 (MQ=255)
                        ggtggtCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTaa                                                                                                                       >  2:3592780/1‑148 (MQ=255)
                        ggtggtCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTaa                                                                                                                       >  2:2491915/1‑148 (MQ=255)
                        ggtggtCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAACCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTaa                                                                                                                       <  1:1655555/148‑1 (MQ=255)
                         gtggtCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTaaa                                                                                                                      <  1:2365145/148‑1 (MQ=255)
                         gtggtCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTaaa                                                                                                                      <  2:1073588/148‑1 (MQ=255)
                          tggtCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAg                                                                                                                     >  2:3536624/1‑148 (MQ=255)
                           ggtCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGt                                                                                                                    <  1:294335/148‑1 (MQ=255)
                            gtCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTc                                                                                                                   >  2:2686503/1‑148 (MQ=255)
                               gtggtgGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCaga                                                                                                                <  2:1771918/148‑1 (MQ=255)
                                tggtggTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCagag                                                                                                               >  2:191178/1‑148 (MQ=255)
                                tggtggTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCagag                                                                                                               >  2:2121490/1‑148 (MQ=255)
                                  gtggtTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTg                                                                                                             >  2:3547661/1‑148 (MQ=255)
                                    ggtTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAg                                                                                                           <  1:621187/148‑1 (MQ=255)
                                     gtTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAgg                                                                                                          <  2:762546/148‑1 (MQ=255)
                                     gtTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAgg                                                                                                          >  2:3771749/1‑148 (MQ=255)
                                       tCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAgggg                                                                                                        >  2:2170376/1‑148 (MQ=255)
                                         tGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAgggggg                                                                                                      >  2:1310819/1‑148 (MQ=255)
                                          ggCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAggggggg                                                                                                     >  2:3236962/1‑148 (MQ=255)
                                            cACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGAt                                                                                                   >  1:2200720/1‑148 (MQ=255)
                                             aCTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGCAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATg                                                                                                  <  1:2904234/148‑1 (MQ=255)
                                              cTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGt                                                                                                 >  2:2316921/1‑148 (MQ=255)
                                                 ttCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTgg                                                                                              >  2:2968752/1‑148 (MQ=255)
                                                     cGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGaa                                                                                          >  1:1466924/1‑148 (MQ=255)
                                                     cGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGaa                                                                                          >  2:3121436/1‑148 (MQ=255)
                                                       aTTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATg                                                                                        >  2:2381064/1‑148 (MQ=255)
                                                       aTTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATg                                                                                        >  1:2079445/1‑148 (MQ=255)
                                                       aTTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATg                                                                                        <  2:522656/148‑1 (MQ=255)
                                                         tAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGtt                                                                                      >  2:3441412/1‑148 (MQ=255)
                                                         tAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGtt                                                                                      >  1:2783283/1‑148 (MQ=255)
                                                            gACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGc                                                                                   <  2:805991/148‑1 (MQ=255)
                                                            gACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGc                                                                                   >  1:2265747/1‑148 (MQ=255)
                                                             aCGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCt                                                                                  >  1:658755/1‑148 (MQ=255)
                                                             aCGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCt                                                                                  >  1:2577266/1‑148 (MQ=255)
                                                                gCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAg                                                                               <  1:500894/148‑1 (MQ=255)
                                                                   cTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGc                                                                            <  1:905484/148‑1 (MQ=255)
                                                                   cTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGc                                                                            >  1:3505040/1‑148 (MQ=255)
                                                                    ttGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGcc                                                                           >  2:662964/1‑148 (MQ=255)
                                                                             tGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTcc                                                                  <  2:2290079/148‑1 (MQ=255)
                                                                             tGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTcc                                                                  >  1:3129953/1‑148 (MQ=255)
                                                                             tGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTcc                                                                  >  1:510724/1‑148 (MQ=255)
                                                                               aaaGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCct                                                                >  2:2578044/1‑148 (MQ=255)
                                                                                 aGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCctct                                                              <  1:2881115/148‑1 (MQ=255)
                                                                                     ccGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCAcc                                                          <  2:229400/148‑1 (MQ=255)
                                                                                     ccGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCAcc                                                          <  2:229619/148‑1 (MQ=255)
                                                                                     ccGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCAcc                                                          <  2:3816688/148‑1 (MQ=255)
                                                                                      cGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCAccc                                                         <  2:396259/148‑1 (MQ=255)
                                                                                      cGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCAccc                                                         <  2:397259/148‑1 (MQ=255)
                                                                                           ccAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAAcc                                                    >  1:2017848/1‑148 (MQ=255)
                                                                                           ccAACGGCGAAAGAAACGCCGTACGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAAcc                                                    <  2:1070115/148‑1 (MQ=255)
                                                                                              aCGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCctc                                                 <  2:2150167/148‑1 (MQ=255)
                                                                                               cGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCctct                                                >  2:3273618/1‑148 (MQ=255)
                                                                                                  cGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTccc                                             <  2:2392148/148‑1 (MQ=255)
                                                                                                    aaagaaaCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCg                                           >  1:1453462/1‑148 (MQ=255)
                                                                                                       gaaaCGCCGTATGAACCACTGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAACGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTg                                        <  2:2556178/148‑1 (MQ=255)
                                                                                                        aaaCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTgg                                       >  1:790460/1‑148 (MQ=255)
                                                                                                          aCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGTGTTCCCTCTCACCCTAACCCTCTCCCCGGTgggg                                     >  2:56050/1‑148 (MQ=255)
                                                                                                          aCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTgggg                                     >  2:2991766/1‑148 (MQ=255)
                                                                                                           cGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGc                                    >  2:2223473/1‑148 (MQ=255)
                                                                                                           cGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGc                                    >  1:3434704/1‑148 (MQ=255)
                                                                                                                 aTGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAgggg                              >  2:42724/1‑148 (MQ=255)
                                                                                                                  tGAACCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGa                             <  1:327937/148‑1 (MQ=255)
                                                                                                                    aaCCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACt                           >  2:3170496/1‑148 (MQ=255)
                                                                                                                    aaCCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACt                           >  1:1110608/1‑148 (MQ=255)
                                                                                                                     aCCAATGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTg                          <  2:1193976/148‑1 (MQ=255)
                                                                                                                         aTGCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCg                      >  1:831973/1‑148 (MQ=255)
                                                                                                                           gCCGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAg                    <  2:510724/148‑1 (MQ=255)
                                                                                                                            ccGGAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAgc                   >  2:3001970/1‑148 (MQ=255)
                                                                                                                               gAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGcgcg                >  2:584957/1‑148 (MQ=255)
                                                                                                                               gAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGcgcg                >  2:1581398/1‑148 (MQ=255)
                                                                                                                               gAAAATGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGcgcg                >  2:1745735/1‑148 (MQ=255)
                                                                                                                                aaaaTGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGt               <  2:3118486/148‑1 (MQ=255)
                                                                                                                                aaaaTGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGt               >  1:3061259/1‑148 (MQ=255)
                                                                                                                                 aaaTGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGtt              <  1:3546981/148‑1 (MQ=255)
                                                                                                                                 aaaTGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGtt              <  1:3547696/148‑1 (MQ=255)
                                                                                                                                   aTGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGa            <  1:1206771/148‑1 (MQ=255)
                                                                                                                                   aTGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGa            >  1:1394657/1‑148 (MQ=255)
                                                                                                                                   aTGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGa            >  1:2430623/1‑148 (MQ=255)
                                                                                                                                    tGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGAt           >  1:349661/1‑148 (MQ=255)
                                                                                                                                    tGTCGAGGAGATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGAt           >  1:248650/1‑148 (MQ=255)
                                                                                                                                       cGAGGAGATAGTGGCGCAGGCAAAATGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGc        <  1:545480/148‑1 (MQ=255)
                                                                                                                                          ggagATAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCAtt     >  1:1851635/1‑148 (MQ=255)
                                                                                                                                           gcgaTAGTGCCGCAGGCAACAGGCGCACACTAAAGTCAGAGTGCGGGGGGGATGTTGGCGAATGTGGGCTTAGTGCCCAGGGGTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCAttt    <  1:1954500/146‑1 (MQ=38)
                                                                                                                                           gagaTAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCAttt    >  1:682485/1‑148 (MQ=255)
                                                                                                                                           gagaTAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCAttt    <  1:1661763/148‑1 (MQ=255)
                                                                                                                                           gagaTAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCAttt    >  2:2279110/1‑148 (MQ=255)
                                                                                                                                             gaTAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGCGCGCGTTGATAGCAGTTGt  >  1:904845/1‑148 (MQ=255)
                                                                                                                                             gaTAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGt  <  1:3698153/148‑1 (MQ=255)
                                                                                                                                             gaTAGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGt  >  2:281731/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGT  >  CP009273/627284‑627572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑