Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 705,588 C→A 100% intergenic (+16/+68) chiQ → / ← fur chitosugar‑induced verified lipoprotein/ferric iron uptake regulon transcriptional repressor; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273705,5880CA99.6% 749.1 / ‑4.3 233intergenic (+16/+68)chiQ/furchitosugar‑induced verified lipoprotein/ferric iron uptake regulon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (116/115);  total (116/117)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 233 total aligned reads displayed.

CCGACCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGT  >  CP009273/705445‑705732
                                                                                                                                               |                                                                                                                                                
    ccAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCtttt                                                                                                                                          <  1:3269133/148‑1 (MQ=255)
    ccAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCtttt                                                                                                                                          <  1:1363423/148‑1 (MQ=255)
     cAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTc                                                                                                                                         <  1:1286203/148‑1 (MQ=255)
      aGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCt                                                                                                                                        <  1:1334488/148‑1 (MQ=255)
       ggAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCtt                                                                                                                                       <  2:3331911/148‑1 (MQ=255)
       ggAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCtt                                                                                                                                       >  2:2827025/1‑148 (MQ=255)
       ggAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCtt                                                                                                                                       >  1:1020456/1‑148 (MQ=255)
        gAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTa                                                                                                                                      >  1:997001/1‑148 (MQ=255)
        gAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTa                                                                                                                                      >  1:2865028/1‑148 (MQ=255)
         aaaGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTAt                                                                                                                                     <  2:395411/148‑1 (MQ=255)
            gtgtACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTc                                                                                                                                  <  1:1181888/148‑1 (MQ=255)
             tgtACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTcc                                                                                                                                 <  1:379525/148‑1 (MQ=255)
              gtACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTccc                                                                                                                                >  2:2721973/1‑148 (MQ=255)
                aCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCtt                                                                                                                              >  2:889901/1‑148 (MQ=255)
                aCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCtt                                                                                                                              >  2:887322/1‑148 (MQ=255)
                aCGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCtt                                                                                                                              >  2:3146231/1‑148 (MQ=255)
                    gTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCAt                                                                                                                          <  1:336964/148‑1 (MQ=255)
                      aCTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATaa                                                                                                                        <  1:543799/148‑1 (MQ=255)
                       cTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATaaa                                                                                                                       >  1:1222183/1‑148 (MQ=255)
                       cTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATaaa                                                                                                                       >  1:877140/1‑148 (MQ=255)
                        tGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATaaaa                                                                                                                      <  1:2688987/148‑1 (MQ=255)
                         ggATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATaaaaa                                                                                                                     >  2:947081/1‑148 (MQ=255)
                         ggATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATaaaaa                                                                                                                     >  2:2858553/1‑148 (MQ=255)
                           aTTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAg                                                                                                                   <  1:1452738/148‑1 (MQ=255)
                             tATCAGCAGTGTCTGCGTGCAACGCAAACCGGACATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGcc                                                                                                                 >  2:2522169/1‑148 (MQ=255)
                             tATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGcc                                                                                                                 >  1:2530641/1‑148 (MQ=255)
                              aTCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCa                                                                                                                <  1:3531013/148‑1 (MQ=255)
                               tCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCaa                                                                                                               <  2:3181967/148‑1 (MQ=255)
                               tCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCaa                                                                                                               <  1:651362/148‑1 (MQ=255)
                                cagcagTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAAc                                                                                                              <  2:3625980/148‑1 (MQ=255)
                                      tgtCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAg                                                                                                        >  1:1226677/1‑148 (MQ=255)
                                       gtCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAgg                                                                                                       <  2:853495/148‑1 (MQ=255)
                                              tGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCtt                                                                                                >  1:2005876/1‑148 (MQ=255)
                                                cAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCtttt                                                                                              <  1:861317/148‑1 (MQ=255)
                                                  aCGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtct                                                                                            >  1:2394811/1‑148 (MQ=255)
                                                   cGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTACATAAAAAAGCCAACCCGCAGGTTGGCTTTtctc                                                                                           >  1:950314/1‑148 (MQ=255)
                                                   cGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCATTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtctc                                                                                           >  2:1596389/1‑148 (MQ=255)
                                                    gCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAAGACGCAGTAATAACAAATACGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCg                                                                                          <  1:3206529/148‑1 (MQ=255)
                                                        aCCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCa                                                                                      >  2:2786959/1‑148 (MQ=255)
                                                        aCCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCa                                                                                      >  1:3127486/1‑148 (MQ=255)
                                                               aTGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAggctggc                                                                               >  2:710317/1‑148 (MQ=255)
                                                                 gATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAATACAACAAGCAGGTTGGCTTTTCTCGTTAATGCTGGCtt                                                                             >  1:3255647/1‑148 (MQ=255)
                                                                 gATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCtt                                                                             >  2:2359630/1‑148 (MQ=255)
                                                                   tCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTAt                                                                           <  2:3206704/148‑1 (MQ=255)
                                                                   tCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTAt                                                                           <  2:2996131/148‑1 (MQ=255)
                                                                      ggCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTg                                                                        >  2:1794597/1‑148 (MQ=255)
                                                                       gCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGc                                                                       <  1:52140/148‑1 (MQ=255)
                                                                          gTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCtt                                                                    <  2:2591105/148‑1 (MQ=255)
                                                                                 ccGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTgcgc                                                             >  1:935613/1‑148 (MQ=255)
                                                                                 ccGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTgcgc                                                             >  2:2104857/1‑148 (MQ=255)
                                                                                 ccGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTgcgc                                                             >  2:2104830/1‑148 (MQ=255)
                                                                                  cGATTGTTATTTAGTCTTGCAGGAATTTCTCAGTAATAACAAATAAGTGAGAGCTGTAACTTTCGCTTTTCTTATTTCCCTTTCATAAAAAAGCCAACCCGCAGGTTGGATTTTCTAGTTCAGGCTGGCTTATTTGCCTTCGTGCGCa                                                            <  2:1298114/148‑1 (MQ=255)
                                                                                    aTTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATg                                                          >  1:2967093/1‑148 (MQ=255)
                                                                                     ttGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGt                                                         <  1:3041276/148‑1 (MQ=255)
                                                                                      tgtgATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGtt                                                        >  2:1856950/1‑148 (MQ=255)
                                                                                      tgtgATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGtt                                                        >  1:1134895/1‑148 (MQ=255)
                                                                                          aTAAAGTCTGGCAGGAAATACGCAGTAATAACAAATCAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATc                                                    <  2:112227/148‑1 (MQ=255)
                                                                                          aTAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATc                                                    <  2:111660/148‑1 (MQ=255)
                                                                                          aTAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATc                                                    >  2:579726/1‑148 (MQ=255)
                                                                                            aaaGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCtt                                                  <  1:2994300/148‑1 (MQ=255)
                                                                                            aaaGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCtt                                                  >  1:2145839/1‑148 (MQ=255)
                                                                                              aGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGATAGCTGAAAATATCGCTTTTATTATTTACCTTGAATAAAAAATCAAACCCTCAGGATGGCTTTTCTCTTTCAGGCTGGCTTATTTGCATTCGTGCGCATGTTCATCttag                                                >  2:2421633/1‑146 (MQ=255)
                                                                                              aGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCGACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTcg                                                <  1:2625506/148‑1 (MQ=255)
                                                                                              aGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTcg                                                >  2:495872/1‑148 (MQ=255)
                                                                                               gTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTcgc                                               >  2:364177/1‑148 (MQ=255)
                                                                                                tCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTcgcg                                              >  1:2877717/1‑148 (MQ=255)
                                                                                                    gCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCaa                                          <  1:904939/148‑1 (MQ=255)
                                                                                                     cAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAAt                                         >  1:3357378/1‑148 (MQ=255)
                                                                                                        ctaaTACGAATTAATAAAAAATAAGTTACAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGc                                      <  2:1136950/146‑1 (MQ=255)
                                                                                                         aaaTACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGcc                                     >  2:450611/1‑148 (MQ=255)
                                                                                                          aaTACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCt                                    <  2:923807/148‑1 (MQ=255)
                                                                                                           aTACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCtt                                   <  2:3380752/148‑1 (MQ=255)
                                                                                                            tACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTc                                  >  1:1008665/1‑148 (MQ=255)
                                                                                                                cAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGca                              <  2:2949465/148‑1 (MQ=255)
                                                                                                                 aGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGcac                             <  1:457314/148‑1 (MQ=255)
                                                                                                                   taataaCAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAg                           >  2:3019840/1‑148 (MQ=255)
                                                                                                                     ataaCAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTg                         <  2:195336/148‑1 (MQ=255)
                                                                                                                      taaCAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGa                        >  1:2288004/1‑148 (MQ=255)
                                                                                                                       aaCAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAATGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGAc                       >  2:1468486/1‑148 (MQ=255)
                                                                                                                       aaCAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGAc                       >  1:1773868/1‑148 (MQ=255)
                                                                                                                       aaCAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGAc                       <  2:1559180/148‑1 (MQ=255)
                                                                                                                           aaTAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTa                   >  2:950765/1‑148 (MQ=255)
                                                                                                                            aTAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTaa                  <  1:587322/148‑1 (MQ=255)
                                                                                                                            aTAAGTGAGAGCTGTAACTATCGCTTTTCTTAGTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTaa                  <  2:3777175/148‑1 (MQ=255)
                                                                                                                              aaGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAg                >  2:1233509/1‑148 (MQ=255)
                                                                                                                              aaGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAg                >  2:2125243/1‑148 (MQ=255)
                                                                                                                               aGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGa               >  2:2290800/1‑148 (MQ=255)
                                                                                                                               aGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGa               <  1:889901/148‑1 (MQ=255)
                                                                                                                                gTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGAt              <  2:473152/148‑1 (MQ=255)
                                                                                                                                gTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGAt              <  1:2485612/148‑1 (MQ=255)
                                                                                                                                 tGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATa             >  1:2167524/1‑148 (MQ=255)
                                                                                                                                 tGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATa             >  1:160989/1‑148 (MQ=255)
                                                                                                                                  gagaGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATag            >  1:2731205/1‑148 (MQ=255)
                                                                                                                                  gagaGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATag            <  1:2722745/148‑1 (MQ=255)
                                                                                                                                  gagaGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATag            <  2:1341246/148‑1 (MQ=255)
                                                                                                                                   agagCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATaga           >  1:392376/1‑148 (MQ=255)
                                                                                                                                     agCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAgaga         <  1:2827025/148‑1 (MQ=255)
                                                                                                                                     agCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAgaga         >  1:1090695/1‑148 (MQ=255)
                                                                                                                                       cTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACt       >  1:3799762/1‑148 (MQ=255)
                                                                                                                                            aCTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGt  >  2:3321458/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                                
CCGACCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGT  >  CP009273/705445‑705732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑