Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 723,859 G→T 100% V110V (GTC→GTA)  kdpA ← potassium translocating ATPase, subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273723,8590GT99.0% 667.1 / ‑1.7 205V110V (GTC→GTA) kdpApotassium translocating ATPase, subunit A
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (96/107);  total (97/108)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 205 total aligned reads displayed.

GCGGGTAAACGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGA  >  CP009273/723718‑724003
                                                                                                                                             |                                                                                                                                                
     tAAACGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGAc                                                                                                                                       <  1:1677786/148‑1 (MQ=255)
        aCGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGAcggc                                                                                                                                    >  2:3814987/1‑148 (MQ=255)
              ggATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTAtt                                                                                                                              <  1:2700901/148‑1 (MQ=255)
               gATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTc                                                                                                                             >  2:1753708/1‑148 (MQ=255)
                aTGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCa                                                                                                                            >  2:1297565/1‑148 (MQ=255)
                    gggCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAgcgc                                                                                                                        <  1:315417/148‑1 (MQ=255)
                          aaaTCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTTCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTGTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATc                                                                                                                  >  1:2847165/1‑148 (MQ=255)
                          aaaTCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATc                                                                                                                  >  1:2601647/1‑148 (MQ=255)
                           aaTCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATcc                                                                                                                 <  1:2500236/148‑1 (MQ=255)
                            aTCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTCTAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATccc                                                                                                                >  2:365313/1‑148 (MQ=255)
                            aTCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATccc                                                                                                                <  2:2838963/148‑1 (MQ=255)
                               aCCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACg                                                                                                             >  2:416690/1‑148 (MQ=255)
                                ccGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGa                                                                                                            >  2:2812936/1‑148 (MQ=255)
                                    aaTCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGc                                                                                                        <  2:2451628/148‑1 (MQ=255)
                                     aTCCCGCTGGCGGCAGAAATAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGcc                                                                                                       <  1:13878/148‑1 (MQ=255)
                                     aTCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTTAAATAGCTCAACGTGGTTTCACCGGTATAAGTTTGCCATTTGGTATTGGTTACAAAGCTTACGGCGGTATTCAGCGCCAGATCCCACGACAGcc                                                                                                       <  1:1105982/148‑1 (MQ=255)
                                     aTCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGTCGGCGGTATTCAGCGCCAGATCCCACGACAGcc                                                                                                       <  2:2498525/148‑1 (MQ=255)
                                     aTCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGcc                                                                                                       <  2:507512/148‑1 (MQ=255)
                                     aTCCCGCTGGCGGCAGAAAGAAAGTTTTGCACAGTTAAGCCCGCCATCTGGCTGAAATAGCTCTACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGcc                                                                                                       <  2:225392/148‑1 (MQ=255)
                                      tCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGccc                                                                                                      >  2:2530356/1‑148 (MQ=255)
                                       cccGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCt                                                                                                     <  2:3597673/148‑1 (MQ=255)
                                           cTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCa                                                                                                 <  2:638549/148‑1 (MQ=255)
                                            tGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCaa                                                                                                <  2:1186605/148‑1 (MQ=255)
                                            tGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCaa                                                                                                <  1:447871/148‑1 (MQ=255)
                                            tGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCaa                                                                                                <  2:832882/148‑1 (MQ=255)
                                              gcggcAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAAct                                                                                              >  2:2360595/1‑148 (MQ=255)
                                               cggcAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAActg                                                                                             <  1:3670725/148‑1 (MQ=255)
                                               cggcAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAActg                                                                                             >  2:2969712/1‑148 (MQ=255)
                                                   agaaagaaAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCtgt                                                                                         <  1:2873685/148‑1 (MQ=255)
                                                   agaaagaaAGTTGTGCACCTTTAAGCCCGCAATCTGGCTGAAATTGCTCATCGTGGTTTCACCGATATAAGTTGGCCTGTTGTTATTGGTTACAAAGGTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCtgt                                                                                         <  1:1297565/148‑1 (MQ=255)
                                                    gaaagaaaGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCtgtg                                                                                        >  1:3470378/1‑148 (MQ=255)
                                                     aaagaaagTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACGAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTgg                                                                                       <  1:1958680/148‑1 (MQ=255)
                                                     aaagaaagTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTgg                                                                                       >  2:2919397/1‑148 (MQ=255)
                                                     aaagaaagTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTgg                                                                                       >  2:343195/1‑148 (MQ=255)
                                                     aaagaaagTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTgg                                                                                       >  1:3718291/1‑148 (MQ=255)
                                                     aaagaaagTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTgg                                                                                       >  1:406771/1‑148 (MQ=255)
                                                     aaagaaagTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTgg                                                                                       >  1:696318/1‑148 (MQ=255)
                                                       agaaagTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGAt                                                                                     >  2:2358377/1‑148 (MQ=255)
                                                        gaaagTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGAtt                                                                                    >  1:1720346/1‑148 (MQ=255)
                                                         aaagTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTa                                                                                   >  1:1829862/1‑148 (MQ=255)
                                                         aaagTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTa                                                                                   >  2:1498662/1‑148 (MQ=255)
                                                          aagTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTaa                                                                                  <  1:1081851/148‑1 (MQ=255)
                                                           agTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAg                                                                                 <  1:3242803/148‑1 (MQ=255)
                                                             ttttGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCg                                                                               >  2:1989054/1‑148 (MQ=255)
                                                              tttGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCgg                                                                              >  2:2802465/1‑148 (MQ=255)
                                                              tttGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCgg                                                                              >  2:217237/1‑148 (MQ=255)
                                                              tttGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCgg                                                                              >  2:2145791/1‑148 (MQ=255)
                                                              tttGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCgg                                                                              >  1:736516/1‑148 (MQ=255)
                                                               ttGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGc                                                                             <  1:21463/148‑1 (MQ=255)
                                                                  cACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGa                                                                          >  2:708345/1‑148 (MQ=255)
                                                                   aCCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGAt                                                                         <  1:3129871/148‑1 (MQ=255)
                                                                    ccGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATa                                                                        >  2:554400/1‑148 (MQ=255)
                                                                    ccGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATa                                                                        >  2:1954322/1‑148 (MQ=255)
                                                                       ttAAGCCCGCCTTCTGGCTTAAATAGCTAAACGTGGTTTCACGGCTATACGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTg                                                                     <  1:1617641/148‑1 (MQ=255)
                                                                       ttAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTg                                                                     <  1:2042754/148‑1 (MQ=255)
                                                                       ttAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTg                                                                     <  1:834425/148‑1 (MQ=255)
                                                                       ttAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTg                                                                     <  1:624703/148‑1 (MQ=255)
                                                                        tAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGc                                                                    >  2:3704962/1‑148 (MQ=255)
                                                                        tAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGc                                                                    >  2:1658586/1‑148 (MQ=255)
                                                                          aGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTg                                                                  <  2:3004327/148‑1 (MQ=255)
                                                                           gCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGa                                                                 <  1:1292844/148‑1 (MQ=255)
                                                                           gCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGa                                                                 >  2:2539251/1‑148 (MQ=255)
                                                                           gCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGa                                                                 >  1:3814966/1‑148 (MQ=255)
                                                                              cGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCg                                                              >  2:631660/1‑148 (MQ=255)
                                                                                 cATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGc                                                           <  2:2284428/148‑1 (MQ=255)
                                                                                  aTCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCa                                                          <  2:3171458/148‑1 (MQ=255)
                                                                                   tCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCaa                                                         >  2:3475392/1‑148 (MQ=255)
                                                                                    cTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAAc                                                        <  2:1844923/148‑1 (MQ=255)
                                                                                    cTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAAc                                                        <  2:3500098/148‑1 (MQ=255)
                                                                                    cTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAAc                                                        <  2:1429621/148‑1 (MQ=255)
                                                                                           ataTAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATaaaaa                                                 <  1:2995920/146‑1 (MQ=255)
                                                                                           aaaTAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATaaaaa                                                 >  1:1432043/1‑148 (MQ=255)
                                                                                           aaaTAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATaaaaa                                                 <  2:3718291/148‑1 (MQ=255)
                                                                                            aaTAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATaaaaaa                                                <  1:1466683/148‑1 (MQ=255)
                                                                                             aTAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAAc                                               <  1:1904704/148‑1 (MQ=255)
                                                                                              tAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACa                                              >  2:3423816/1‑148 (MQ=255)
                                                                                                 cTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCa                                           >  1:742850/1‑148 (MQ=255)
                                                                                                  tCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCAc                                          >  1:1462633/1‑148 (MQ=255)
                                                                                                         ggTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGc                                   <  1:2423848/148‑1 (MQ=255)
                                                                                                            ttCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGcccc                                >  1:1781583/1‑148 (MQ=255)
                                                                                                               aCCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGc                             <  1:1790026/148‑1 (MQ=255)
                                                                                                               aCCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGc                             >  2:1392243/1‑148 (MQ=255)
                                                                                                                ccGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCa                            >  1:306400/1‑148 (MQ=255)
                                                                                                                ccGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCa                            >  1:2233357/1‑148 (MQ=255)
                                                                                                                 cGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCAt                           >  2:3685934/1‑148 (MQ=255)
                                                                                                                  gCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATg                          <  1:3168709/148‑1 (MQ=255)
                                                                                                                   cTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGt                         <  1:1269826/148‑1 (MQ=255)
                                                                                                                        aGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAgg                    >  1:2665699/1‑148 (MQ=255)
                                                                                                                          aTTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGcc                  >  1:3040569/1‑148 (MQ=255)
                                                                                                                            tGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCga                <  1:725848/148‑1 (MQ=255)
                                                                                                                              ccAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCgaga              <  2:677213/148‑1 (MQ=255)
                                                                                                                               cAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGaa             <  1:1391716/148‑1 (MQ=255)
                                                                                                                                aGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAAt            >  2:1091167/1‑148 (MQ=255)
                                                                                                                                   tGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGc         <  2:3018911/148‑1 (MQ=255)
                                                                                                                                      tATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGcaca      >  1:950776/1‑148 (MQ=255)
                                                                                                                                       aTTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACaa     >  2:126949/1‑148 (MQ=255)
                                                                                                                                       aTTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACaa     <  2:736516/148‑1 (MQ=255)
                                                                                                                                       aTTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACaa     <  2:822344/148‑1 (MQ=255)
                                                                                                                                       aTTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCAGGTTCAGGCCGAGAATGGCACaa     >  2:126544/1‑148 (MQ=255)
                                                                                                                                          ggTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGa  <  2:1015266/148‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                
GCGGGTAAACGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGA  >  CP009273/723718‑724003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑