Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 748,707 A→T 100% I406I (ATT→ATA)  gltA ← citrate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273748,7070AT96.6% 760.3 / 12.0 235I406I (ATT→ATA) gltAcitrate synthase
Reads supporting (aligned to +/- strand):  ref base A (3/5);  new base T (113/114);  total (116/119)
Fisher's exact test for biased strand distribution p-value = 7.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 236 total aligned reads displayed.

ATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTAC  >  CP009273/748561‑748851
                                                                                                                                                  |                                                                                                                                                
aTTTTTTGTGTTTAGGCATATAAAAAACAACCCGCCATATGAACGGCGGGTTAAAATAATTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCaa                                                                                                                                                 >  1:2215486/1‑148 (MQ=255)
 ttttttGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTAt                                                                                                                                                >  2:3713939/1‑148 (MQ=255)
  tttttGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATc                                                                                                                                               <  2:1479117/148‑1 (MQ=255)
       gtgtTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCAt                                                                                                                                          >  2:169261/1‑148 (MQ=255)
        tgtTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATa                                                                                                                                         >  2:469868/1‑148 (MQ=255)
         gtTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATAc                                                                                                                                        >  2:175688/1‑148 (MQ=255)
           ttAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCg                                                                                                                                      >  2:826678/1‑148 (MQ=255)
           ttAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCg                                                                                                                                      >  1:1009270/1‑148 (MQ=255)
           ttAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCg                                                                                                                                      >  1:62963/1‑148 (MQ=255)
           ttAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCg                                                                                                                                      >  2:1821221/1‑148 (MQ=255)
           ttAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCg                                                                                                                                      >  1:1981498/1‑148 (MQ=255)
              ggCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTAATACCGTCa                                                                                                                                   >  1:3261252/1‑148 (MQ=255)
              ggCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATACTGTATACATCTGACGCGGAAGGGCTATCTTCATACCGTCa                                                                                                                                   >  1:3261785/1‑148 (MQ=255)
               gCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCAc                                                                                                                                  >  1:381553/1‑148 (MQ=255)
                  tataAAAATCAACCCGCCATATGCACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACtgt                                                                                                                               <  2:261394/148‑1 (MQ=255)
                  tataAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACtgt                                                                                                                               >  1:2048363/1‑148 (MQ=255)
                        aaTCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCAttt                                                                                                                         <  2:1614064/148‑1 (MQ=255)
                        aaTCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCAttt                                                                                                                         >  2:775648/1‑148 (MQ=255)
                         aTCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGGGGGCGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTc                                                                                                                        >  2:3635652/1‑148 (MQ=255)
                          tCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCg                                                                                                                       <  1:1191066/148‑1 (MQ=255)
                          tCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCg                                                                                                                       <  1:924738/148‑1 (MQ=255)
                             aCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTc                                                                                                                    <  2:1998068/148‑1 (MQ=255)
                             aCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTc                                                                                                                    >  2:1533465/1‑148 (MQ=255)
                              cccGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTcc                                                                                                                   >  2:2321837/1‑148 (MQ=255)
                              cccGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTcc                                                                                                                   >  1:701306/1‑148 (MQ=255)
                              cccGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTcc                                                                                                                   >  1:821395/1‑148 (MQ=255)
                                  ccATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCGCCAGTg                                                                                                               >  2:2136715/1‑148 (MQ=255)
                                    atatGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTggg                                                                                                             <  1:2874856/148‑1 (MQ=255)
                                    atatGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTggg                                                                                                             <  1:3595524/148‑1 (MQ=255)
                                      atGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCg                                                                                                           >  1:3550236/1‑148 (MQ=255)
                                       tGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGa                                                                                                          >  2:2155924/1‑148 (MQ=255)
                                        gAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCAGTTCGCTCCAGTGGGCGAt                                                                                                         <  1:1425525/148‑1 (MQ=255)
                                          aCGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATcc                                                                                                       <  1:3298842/148‑1 (MQ=255)
                                             gcggGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGc                                                                                                    <  1:2588407/148‑1 (MQ=255)
                                             gcggGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGc                                                                                                    <  1:2588411/148‑1 (MQ=255)
                                             gcggGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGc                                                                                                    <  2:1509177/148‑1 (MQ=255)
                                                ggTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCaa                                                                                                 >  1:3137708/1‑148 (MQ=255)
                                                  ttAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACg                                                                                               <  2:1629742/148‑1 (MQ=255)
                                                    aaaaTATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGt                                                                                             <  1:645868/148‑1 (MQ=255)
                                                       atatTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGAAGGGCTATCTTCATACCGTCACTGTGCATTTAGCTCCAGTGGGCGATCCAGCCAACGGTACg                                                                                          >  1:3452571/1‑148 (MQ=255)
                                                        tatTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGt                                                                                         >  1:610721/1‑148 (MQ=255)
                                                         atTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTg                                                                                        >  1:2026793/1‑148 (MQ=255)
                                                          tttACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGc                                                                                       <  2:1842669/148‑1 (MQ=255)
                                                            tACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCa                                                                                     <  2:2495520/148‑1 (MQ=255)
                                                               aaCTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTg                                                                                  <  2:3283323/148‑1 (MQ=255)
                                                                        aTCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGa                                                                         <  1:24663/148‑1 (MQ=255)
                                                                          cAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACg                                                                       <  1:3635652/148‑1 (MQ=255)
                                                                            aCCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGt                                                                     <  1:469868/148‑1 (MQ=255)
                                                                              cATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGa                                                                   <  2:3369894/148‑1 (MQ=255)
                                                                               aTTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGaa                                                                  >  2:1938024/1‑148 (MQ=255)
                                                                                ttAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAAc                                                                 >  1:3428207/1‑148 (MQ=255)
                                                                                 tAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACa                                                                >  2:1995992/1‑148 (MQ=255)
                                                                                  aaCGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACAt                                                               <  2:3187305/148‑1 (MQ=255)
                                                                                    cGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATgg                                                             >  2:1588431/1‑148 (MQ=255)
                                                                                       ttGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAg                                                          <  1:826678/148‑1 (MQ=255)
                                                                                       ttGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAg                                                          <  1:3341001/148‑1 (MQ=255)
                                                                                       ttGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAg                                                          <  1:3513826/148‑1 (MQ=255)
                                                                                        tGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGa                                                         <  1:115322/148‑1 (MQ=255)
                                                                                        tGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGa                                                         <  2:3791880/148‑1 (MQ=255)
                                                                                         gATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGAc                                                        <  2:381553/148‑1 (MQ=255)
                                                                                          atatCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACg                                                       >  2:245211/1‑148 (MQ=255)
                                                                                          atatCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACg                                                       >  2:244892/1‑148 (MQ=255)
                                                                                            atCGCTTTTAAAGTCGCGTTTTTCATATCCTGTTTACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGa                                                     <  2:1304402/148‑1 (MQ=255)
                                                                                             tCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGaa                                                    >  1:3689720/1‑148 (MQ=255)
                                                                                             tCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGaa                                                    >  1:194418/1‑148 (MQ=255)
                                                                                             tCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGaa                                                    <  1:2954405/148‑1 (MQ=255)
                                                                                              cGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAAt                                                   >  2:284938/1‑148 (MQ=255)
                                                                                               gCTTTTAAAGTCGCGTTTTTCATATCCTGTAGACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATa                                                  <  1:2319383/148‑1 (MQ=255)
                                                                                                    tAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGTGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCAt                                             <  1:3426468/148‑1 (MQ=255)
                                                                                                        gTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCt                                         >  1:941583/1‑148 (MQ=255)
                                                                                                        gTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCt                                         <  2:2173492/148‑1 (MQ=255)
                                                                                                              tttttCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGACGACGGAATACCCATCGCTTTCAgg                                   <  1:1504103/148‑1 (MQ=255)
                                                                                                               ttttCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGga                                  >  1:2679354/1‑148 (MQ=255)
                                                                                                               ttttCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGga                                  <  2:142122/148‑1 (MQ=255)
                                                                                                                  tCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGgatga                               <  1:1794315/148‑1 (MQ=255)
                                                                                                                    atatCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATa                             <  1:1588372/148‑1 (MQ=255)
                                                                                                                     tatCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATAc                            >  1:1762854/1‑148 (MQ=255)
                                                                                                                        ccTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCag                         <  2:2091337/148‑1 (MQ=255)
                                                                                                                        ccTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCag                         >  1:1230542/1‑148 (MQ=255)
                                                                                                                        ccTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCag                         >  2:296804/1‑148 (MQ=255)
                                                                                                                         cTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCaga                        <  1:1821225/148‑1 (MQ=255)
                                                                                                                           gTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGt                      >  2:2767180/1‑148 (MQ=255)
                                                                                                                           gTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGt                      <  2:2215486/148‑1 (MQ=255)
                                                                                                                             ataCAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAg                    >  2:1907759/1‑148 (MQ=255)
                                                                                                                               aCAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGaa                  >  2:2517574/1‑148 (MQ=255)
                                                                                                                                cAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGaaa                 <  2:3436453/148‑1 (MQ=255)
                                                                                                                                 aGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAAt                <  2:2701662/148‑1 (MQ=255)
                                                                                                                                   cTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCg              >  1:1958217/1‑148 (MQ=255)
                                                                                                                                   cTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCg              >  1:2345456/1‑148 (MQ=255)
                                                                                                                                   cTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCg              <  1:2778170/148‑1 (MQ=255)
                                                                                                                                    tGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGa             <  2:56085/148‑1 (MQ=255)
                                                                                                                                      aCGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACg           <  2:578692/148‑1 (MQ=255)
                                                                                                                                       cgcgGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGt          <  2:308768/148‑1 (MQ=255)
                                                                                                                                         cgGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTc        <  2:3550236/148‑1 (MQ=255)
                                                                                                                                         cgGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTc        <  1:385433/148‑1 (MQ=255)
                                                                                                                                         cgGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTc        <  1:385230/148‑1 (MQ=255)
                                                                                                                                         cgGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTc        <  2:188474/148‑1 (MQ=255)
                                                                                                                                            aCGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTCGAAATCGACGTTCggg     >  1:3816708/1‑148 (MQ=255)
                                                                                                                                            aCGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCggg     <  1:1533513/148‑1 (MQ=255)
                                                                                                                                               ggCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTAc  >  1:1227803/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
ATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTAC  >  CP009273/748561‑748851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑