Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 754,572 G→T 100% E137D (GAG→GAT)  sucA → 2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273754,5720GT100.0% 648.2 / ‑6.7 199E137D (GAG→GAT) sucA2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (104/94);  total (104/95)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 199 total aligned reads displayed.

AGGTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCA  >  CP009273/754427‑754715
                                                                                                                                                 |                                                                                                                                               
aGGTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATAc                                                                                                                                               <  1:436522/148‑1 (MQ=255)
 ggTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATAcc                                                                                                                                              >  2:851171/1‑148 (MQ=255)
 ggTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATAcc                                                                                                                                              <  2:3014715/148‑1 (MQ=255)
 ggTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATAcc                                                                                                                                              <  1:3151473/148‑1 (MQ=255)
  gTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCt                                                                                                                                             <  1:20341/148‑1 (MQ=255)
   ttAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCtt                                                                                                                                            >  2:3755409/1‑148 (MQ=255)
     aaaGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACCTCCAGGATACCTTCa                                                                                                                                          <  1:1378072/148‑1 (MQ=255)
      aaGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCaa                                                                                                                                         >  1:3148726/1‑148 (MQ=255)
      aaGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCaa                                                                                                                                         >  1:252552/1‑148 (MQ=255)
       aGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAAc                                                                                                                                        >  2:1314749/1‑148 (MQ=255)
       aGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAAc                                                                                                                                        >  1:2620861/1‑148 (MQ=255)
       aGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAAc                                                                                                                                        >  1:2442527/1‑148 (MQ=255)
         tCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGt                                                                                                                                      >  1:1324511/1‑148 (MQ=255)
         tCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGt                                                                                                                                      <  1:2867034/148‑1 (MQ=255)
         tCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGt                                                                                                                                      <  1:873399/148‑1 (MQ=255)
           cTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCg                                                                                                                                    <  2:1356558/148‑1 (MQ=255)
            tGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCgg                                                                                                                                   <  2:1564252/148‑1 (MQ=255)
              cAGCTCATTAACGCATATCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGtt                                                                                                                                 >  1:1156180/1‑148 (MQ=255)
                 cTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCAt                                                                                                                              <  2:2060971/148‑1 (MQ=255)
                 cTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCAt                                                                                                                              <  2:1506759/148‑1 (MQ=255)
                 cTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCAt                                                                                                                              >  2:2499974/1‑148 (MQ=255)
                 cTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCAt                                                                                                                              >  2:525560/1‑148 (MQ=255)
                 cTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTGCCAGGATACCTTCAACGTCGGTTCAt                                                                                                                              >  1:769452/1‑148 (MQ=255)
                  tCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCAtt                                                                                                                             <  2:2117599/148‑1 (MQ=255)
                  tCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCAtt                                                                                                                             <  2:1291861/148‑1 (MQ=255)
                  tCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCAtt                                                                                                                             <  1:1539878/148‑1 (MQ=255)
                    aTTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTg                                                                                                                           >  1:2184239/1‑148 (MQ=255)
                        aCGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAg                                                                                                                       >  2:987663/1‑148 (MQ=255)
                        aCGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAg                                                                                                                       >  2:76387/1‑148 (MQ=255)
                           cATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCgg                                                                                                                    <  2:2630621/148‑1 (MQ=255)
                                  cTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGaa                                                                                                             <  1:743408/148‑1 (MQ=255)
                                    tCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAAc                                                                                                           <  1:2750219/148‑1 (MQ=255)
                                    tCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAAc                                                                                                           >  1:19605/1‑148 (MQ=255)
                                     ccGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAAcc                                                                                                          <  2:1984743/148‑1 (MQ=255)
                                      cGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCa                                                                                                         <  2:1391263/148‑1 (MQ=255)
                                       gTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCAt                                                                                                        <  2:147260/148‑1 (MQ=255)
                                          gTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGaa                                                                                                     <  1:1306417/148‑1 (MQ=255)
                                           tCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGaaa                                                                                                    <  1:1657478/148‑1 (MQ=255)
                                           tCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGaaa                                                                                                    <  2:2212778/148‑1 (MQ=255)
                                           tCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATCaaa                                                                                                    <  1:780959/148‑1 (MQ=255)
                                               cAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCg                                                                                                <  2:348120/148‑1 (MQ=255)
                                                       gAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAgctg                                                                                        <  1:3605493/148‑1 (MQ=255)
                                                        aaTCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAgctgc                                                                                       >  1:3580472/1‑148 (MQ=255)
                                                         aTCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAgctgct                                                                                      <  2:2235636/148‑1 (MQ=255)
                                                             cGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGaa                                                                                  >  1:1004913/1‑148 (MQ=255)
                                                             cGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATAACTTCAACGACGGTTCATTTGCCAGCGGCAAAGAAACCATGAACCTCGGCGAGCTGCTGGaa                                                                                  >  1:2550273/1‑148 (MQ=255)
                                                                tCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCTAGCTGCTGGAAGcc                                                                               >  1:369226/1‑148 (MQ=255)
                                                                    cTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCa                                                                           >  2:2340228/1‑148 (MQ=255)
                                                                            gtgGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAAcc                                                                   >  2:111607/1‑148 (MQ=255)
                                                                               gcagcaAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTAc                                                                <  2:3605133/148‑1 (MQ=255)
                                                                               gcagcaAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTAc                                                                <  1:3755409/148‑1 (MQ=255)
                                                                                     aGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGc                                                          >  2:3248800/1‑148 (MQ=255)
                                                                                      gATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGcc                                                         <  2:917808/148‑1 (MQ=255)
                                                                                      gATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGcc                                                         <  2:2700185/148‑1 (MQ=255)
                                                                                      gATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGcc                                                         <  1:2689391/148‑1 (MQ=255)
                                                                                       aTAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGccc                                                        >  1:2576405/1‑148 (MQ=255)
                                                                                         aaaGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGa                                                      >  2:1368842/1‑148 (MQ=255)
                                                                                         aaaGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGa                                                      >  1:2626663/1‑148 (MQ=255)
                                                                                         aaaGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTAATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGa                                                      <  1:2772952/148‑1 (MQ=255)
                                                                                           aGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGAtt                                                    >  1:1117190/1‑148 (MQ=255)
                                                                                           aGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGAtt                                                    >  1:462750/1‑148 (MQ=255)
                                                                                           aGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGAtt                                                    >  1:2359376/1‑148 (MQ=255)
                                                                                             tGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTgg                                                  <  1:1621500/148‑1 (MQ=255)
                                                                                             tGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTgg                                                  <  1:3318339/148‑1 (MQ=255)
                                                                                               gCCGCTCCGGCTCCCTCTTTCCACGCTCTGACCGAAGCAGACTTCCAGGCGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTg                                                <  2:2550273/148‑1 (MQ=255)
                                                                                               gCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTg                                                <  2:2001206/148‑1 (MQ=255)
                                                                                                ccGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGc                                               <  1:2268469/148‑1 (MQ=255)
                                                                                                   aTCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGa                                            >  1:159030/1‑148 (MQ=255)
                                                                                                    tCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAg                                           >  2:3015759/1‑148 (MQ=255)
                                                                                                    tCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAg                                           >  2:1190735/1‑148 (MQ=255)
                                                                                                      tGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGta                                         >  1:1909860/1‑148 (MQ=255)
                                                                                                       ggATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGtat                                        >  2:2303265/1‑148 (MQ=255)
                                                                                                        gATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGtata                                       <  1:580980/148‑1 (MQ=255)
                                                                                                        gATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGtata                                       >  2:2795522/1‑148 (MQ=255)
                                                                                                        gATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGtata                                       >  2:1277026/1‑148 (MQ=255)
                                                                                                         aTCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTatat                                      >  1:1104738/1‑148 (MQ=255)
                                                                                                          tCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCCAACCTACTGCGGCCCGATTGGTGCCGAGGATATg                                     >  2:2856053/1‑148 (MQ=255)
                                                                                                          tCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATg                                     >  1:893904/1‑148 (MQ=255)
                                                                                                          tCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATg                                     <  2:2167654/148‑1 (MQ=255)
                                                                                                          tCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATg                                     <  2:3282246/148‑1 (MQ=255)
                                                                                                           ccGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGTCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGc                                    <  2:17460/148‑1 (MQ=255)
                                                                                                             gTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGTCGAGTATATGcac                                  <  2:1156467/148‑1 (MQ=255)
                                                                                                              tCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGcaca                                 >  2:2267708/1‑148 (MQ=255)
                                                                                                              tCTTTCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGcaca                                 >  1:156628/1‑148 (MQ=255)
                                                                                                                  tCCACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTAc                             <  2:3772082/148‑1 (MQ=255)
                                                                                                                   ccACGATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTAcc                            >  2:507295/1‑148 (MQ=255)
                                                                                                                       gATCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCa                        <  1:740819/148‑1 (MQ=255)
                                                                                                                        aTCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCAc                       >  2:355753/1‑148 (MQ=255)
                                                                                                                         tCTGACCGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCAcc                      >  1:1777639/1‑148 (MQ=255)
                                                                                                                              ccGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCgaaga                 <  1:1719612/148‑1 (MQ=255)
                                                                                                                               cGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCgaagaa                >  1:3599361/1‑148 (MQ=255)
                                                                                                                               cGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCgaagaa                >  2:2160745/1‑148 (MQ=255)
                                                                                                                               cGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCgaagaa                <  1:1751446/148‑1 (MQ=255)
                                                                                                                               cGAAGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCgaagaa                >  1:2562378/1‑148 (MQ=255)
                                                                                                                                 aaGCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGaaaa              >  1:468524/1‑148 (MQ=255)
                                                                                                                                   gCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAAc            >  1:778212/1‑148 (MQ=255)
                                                                                                                                   gCAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAAc            >  2:3166651/1‑148 (MQ=255)
                                                                                                                                    cAGACTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACg           <  2:1480834/148‑1 (MQ=255)
                                                                                                                                        cTTCCAGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTgg       >  1:338889/1‑148 (MQ=255)
                                                                                                                                             aGGATACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCa  >  1:588658/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
AGGTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCA  >  CP009273/754427‑754715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑