Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 784,094 G→T 100% A67E (GCG→GAG)  galM ← aldose 1‑epimerase; type‑1 mutarotase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273784,0940GT100.0% 763.3 / NA 229A67E (GCG→GAG) galMaldose 1‑epimerase; type‑1 mutarotase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (138/91);  total (138/91)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 229 total aligned reads displayed.

AGCGACGTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACGCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCGTTGTTACGCAAA  >  CP009273/783951‑784237
                                                                                                                                               |                                                                                                                                               
aGCGACGTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGc                                                                                                                                             <  1:1025125/148‑1 (MQ=255)
aGCGACGTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGc                                                                                                                                             <  1:2931465/148‑1 (MQ=255)
 gCGACGTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGcc                                                                                                                                            <  2:3424870/148‑1 (MQ=255)
  cGACGTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGcct                                                                                                                                           >  1:63692/1‑148 (MQ=255)
  cGACGTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGcct                                                                                                                                           >  2:3816763/1‑148 (MQ=255)
    aCGTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGcctga                                                                                                                                         >  2:96741/1‑148 (MQ=255)
      gTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGcctgatc                                                                                                                                       <  2:456033/148‑1 (MQ=255)
       tttGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGcctgatcc                                                                                                                                      <  2:619206/148‑1 (MQ=255)
       tttGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGcctgatcc                                                                                                                                      <  2:3342224/148‑1 (MQ=255)
       tttGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGcctgatcc                                                                                                                                      >  1:929681/1‑148 (MQ=255)
         tGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGcctgatcctg                                                                                                                                    <  1:3234527/148‑1 (MQ=255)
           tCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGcctgatcctgat                                                                                                                                  <  1:769203/148‑1 (MQ=255)
            cGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGCTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATa                                                                                                                                 >  1:3499280/1‑148 (MQ=255)
                cccTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCAt                                                                                                                             <  2:164363/148‑1 (MQ=255)
                cccTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCAt                                                                                                                             <  2:164495/148‑1 (MQ=255)
                cccTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCAt                                                                                                                             >  2:1470493/1‑148 (MQ=255)
                 ccTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCAtt                                                                                                                            >  2:246475/1‑148 (MQ=255)
                 ccTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCAtt                                                                                                                            >  2:3247719/1‑148 (MQ=255)
                     ccGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCgg                                                                                                                        <  1:329551/148‑1 (MQ=255)
                      cGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCggg                                                                                                                       <  2:2103517/148‑1 (MQ=255)
                        gCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCt                                                                                                                     <  2:1766179/148‑1 (MQ=255)
                            gccgTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGca                                                                                                                 <  1:552248/148‑1 (MQ=255)
                             ccgTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGcac                                                                                                                >  1:204545/1‑148 (MQ=255)
                             ccgTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGcac                                                                                                                >  2:571574/1‑148 (MQ=255)
                                  cAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCg                                                                                                           <  1:2416151/148‑1 (MQ=255)
                                     cTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGagc                                                                                                        >  2:1710485/1‑148 (MQ=255)
                                     cTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGagc                                                                                                        >  2:2277318/1‑148 (MQ=255)
                                     cTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGagc                                                                                                        >  2:881485/1‑148 (MQ=255)
                                       ggTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGagcag                                                                                                      <  1:2010169/148‑1 (MQ=255)
                                          tAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAgcgc                                                                                                   >  2:3627778/1‑148 (MQ=255)
                                             cGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTc                                                                                                >  2:1434717/1‑148 (MQ=255)
                                              gCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTcg                                                                                               <  2:288767/148‑1 (MQ=255)
                                               cccTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTcgc                                                                                              >  1:845679/1‑148 (MQ=255)
                                                ccTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTcgcg                                                                                             <  2:113588/148‑1 (MQ=255)
                                                 cTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCgg                                                                                            <  1:142857/148‑1 (MQ=255)
                                                  tGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGa                                                                                           >  1:800092/1‑148 (MQ=255)
                                                  tGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGa                                                                                           >  2:287050/1‑148 (MQ=255)
                                                  tGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGa                                                                                           >  2:2342863/1‑148 (MQ=255)
                                                   gACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGAc                                                                                          >  2:2314064/1‑148 (MQ=255)
                                                    aCTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACg                                                                                         <  2:2717790/148‑1 (MQ=255)
                                                    aCTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATAGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCACGCGGACg                                                                                         >  1:2221821/1‑148 (MQ=255)
                                                     cTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGc                                                                                        >  1:2838164/1‑148 (MQ=255)
                                                      ttGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCt                                                                                       >  2:3648177/1‑148 (MQ=255)
                                                      ttGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCt                                                                                       >  2:1373643/1‑148 (MQ=255)
                                                         gCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGcc                                                                                    >  2:279592/1‑148 (MQ=255)
                                                            aaaGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATc                                                                                 <  1:3661706/148‑1 (MQ=255)
                                                             aaGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCg                                                                                >  1:702990/1‑148 (MQ=255)
                                                               gCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGa                                                                              >  2:3555850/1‑148 (MQ=255)
                                                                cGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGaa                                                                             >  1:476364/1‑148 (MQ=255)
                                                                cGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGaa                                                                             <  1:2169072/148‑1 (MQ=255)
                                                                cGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGaa                                                                             <  1:1852008/148‑1 (MQ=255)
                                                                cGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGaa                                                                             <  1:2286748/148‑1 (MQ=255)
                                                                      ggTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGa                                                                       <  2:2129543/148‑1 (MQ=255)
                                                                         ttCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATa                                                                    >  2:2855876/1‑148 (MQ=255)
                                                                           cACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACg                                                                  <  2:1264506/148‑1 (MQ=255)
                                                                             ccGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACggg                                                                >  2:3139977/1‑148 (MQ=255)
                                                                             ccGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACggg                                                                >  1:3716142/1‑148 (MQ=255)
                                                                             ccGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACggg                                                                <  2:929681/148‑1 (MQ=255)
                                                                                 cAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGa                                                            >  1:985451/1‑148 (MQ=255)
                                                                                  aaaGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGaa                                                           <  1:3385199/148‑1 (MQ=255)
                                                                                   aaGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGaaa                                                          >  1:1831271/1‑148 (MQ=255)
                                                                                    aGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGaaag                                                         >  2:1800660/1‑148 (MQ=255)
                                                                                     ggTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGaaagt                                                        >  1:2206215/1‑148 (MQ=255)
                                                                                     ggTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGaaagt                                                        >  2:2996845/1‑148 (MQ=255)
                                                                                      gTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGACTACGGGCGGaaagta                                                       >  1:2620236/1‑148 (MQ=255)
                                                                                      gTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGaaagta                                                       >  2:2681221/1‑148 (MQ=255)
                                                                                      gTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGaaagta                                                       >  2:765046/1‑148 (MQ=255)
                                                                                        ataACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCCTCGGAAAGCGGAATACGGGCGGacagtaaa                                                     >  1:2064356/1‑148 (MQ=255)
                                                                                        ataACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGaaagtaaa                                                     >  1:488569/1‑148 (MQ=255)
                                                                                          aaCGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGaaagtaaagt                                                   >  2:1570825/1‑148 (MQ=255)
                                                                                          aaCGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGaaagtaaagt                                                   >  2:3721343/1‑148 (MQ=255)
                                                                                          aaCGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGaaagtaaagt                                                   <  2:3411193/148‑1 (MQ=255)
                                                                                          aaCGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCCGACGCTGCCAACGGAAAGCGGAATACGGGCGGaaagtaaagt                                                   >  1:169043/1‑148 (MQ=255)
                                                                                                tATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCAcc                                             <  2:2063939/148‑1 (MQ=255)
                                                                                                 aTTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCAccc                                            >  2:1229750/1‑148 (MQ=255)
                                                                                                 aTTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCAccc                                            >  2:537243/1‑148 (MQ=255)
                                                                                                   tGGCGATACGGTTGGCATAACGACCAATATAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCGGCCCTCGGAAAGCGGAATACGGGCGGAAAGTAAATTCGCACCCCa                                          >  1:109681/1‑148 (MQ=255)
                                                                                                     gCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGt                                        <  1:1226237/148‑1 (MQ=255)
                                                                                                      cGATACGGTTGGCATAACGACCAAGAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTc                                       <  2:124782/148‑1 (MQ=255)
                                                                                                        aTACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCa                                     >  2:3717287/1‑148 (MQ=255)
                                                                                                                ggCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTg                             <  2:3600337/148‑1 (MQ=255)
                                                                                                                 gCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGa                            >  2:3083010/1‑148 (MQ=255)
                                                                                                                    tAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTa                         <  1:130144/148‑1 (MQ=255)
                                                                                                                    tAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTa                         <  2:792073/148‑1 (MQ=255)
                                                                                                                     aaCGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTAc                        >  1:1677061/1‑148 (MQ=255)
                                                                                                                       cGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCa                      >  1:2998857/1‑148 (MQ=255)
                                                                                                                          ccAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATcc                   >  2:2536687/1‑148 (MQ=255)
                                                                                                                          ccAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATcc                   <  1:3663383/148‑1 (MQ=255)
                                                                                                                              tAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGc               >  1:1157199/1‑148 (MQ=255)
                                                                                                                                gagGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCgt             <  1:2336878/148‑1 (MQ=255)
                                                                                                                                gagGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCgt             <  2:1831271/148‑1 (MQ=255)
                                                                                                                                 agGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCgtt            >  1:1354759/1‑148 (MQ=255)
                                                                                                                                  ggCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCgttg           <  1:2797059/148‑1 (MQ=255)
                                                                                                                                  ggCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCgttg           <  2:2374622/148‑1 (MQ=255)
                                                                                                                                  ggCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCgttg           <  1:504784/148‑1 (MQ=255)
                                                                                                                                   gCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCgttgt          <  1:3816763/148‑1 (MQ=255)
                                                                                                                                   gCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCgttgt          >  2:3131375/1‑148 (MQ=255)
                                                                                                                                   gCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCggtgt          >  1:3744590/1‑148 (MQ=255)
                                                                                                                                      cccAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCGTTGTTAc       >  2:510156/1‑148 (MQ=255)
                                                                                                                                          gAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCGTTGTTACGCaa   >  2:2026412/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                               
AGCGACGTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACGCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCGTTGTTACGCAAA  >  CP009273/783951‑784237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑