Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 820,503 C→A 100% T354T (ACG→ACT)  ybhR ← inner membrane putative ABC superfamily transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273820,5030CA99.0% 660.7 / ‑1.7 203T354T (ACG→ACT) ybhRinner membrane putative ABC superfamily transporter permease
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (114/86);  total (115/88)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 203 total aligned reads displayed.

TTGTCCCGCGTTCTGGAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTT  >  CP009273/820359‑820646
                                                                                                                                                |                                                                                                                                               
ttgtCCCGCGTTCTGGAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTg                                                                                                                                              >  1:6503/1‑148 (MQ=255)
ttgtCCCGCGTTCTGGAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTg                                                                                                                                              >  1:3256025/1‑148 (MQ=255)
 tgtCCCGCGTTCTGGAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTgg                                                                                                                                             >  2:1199799/1‑148 (MQ=255)
     ccGCGTTCTGGAAAACAACTTTGGGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGt                                                                                                                                         <  2:182137/148‑1 (MQ=255)
       gcgTTCTGGAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTa                                                                                                                                       >  1:1968392/1‑148 (MQ=255)
        cgTTCTGGAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTAt                                                                                                                                      >  1:512833/1‑148 (MQ=255)
        cgTTCTGGAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTAt                                                                                                                                      >  1:1431303/1‑148 (MQ=255)
         gTTCTGGAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATc                                                                                                                                     <  1:2613024/148‑1 (MQ=255)
             tGGAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCa                                                                                                                                 <  1:3022777/148‑1 (MQ=255)
               gAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGt                                                                                                                               >  1:946373/1‑148 (MQ=255)
               gAAAACAACTTTTTGCGGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGt                                                                                                                               <  2:24187/148‑1 (MQ=255)
                 aaaCAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAg                                                                                                                             >  1:1263182/1‑148 (MQ=255)
                  aacaacTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGc                                                                                                                            >  2:1505982/1‑148 (MQ=255)
                  aacaacTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGc                                                                                                                            >  2:1717762/1‑148 (MQ=255)
                    caacTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCgg                                                                                                                          >  1:2220252/1‑148 (MQ=255)
                     aacTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGc                                                                                                                         <  1:1752132/148‑1 (MQ=255)
                             gCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACt                                                                                                                 <  2:2705585/148‑1 (MQ=255)
                               tGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTAt                                                                                                               <  1:351244/148‑1 (MQ=255)
                                ggCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTAtt                                                                                                              >  1:1678936/1‑148 (MQ=255)
                                       gCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCcacaca                                                                                                       <  1:3064631/148‑1 (MQ=255)
                                       gCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCcacaca                                                                                                       <  1:437064/148‑1 (MQ=255)
                                         aGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCGTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAat                                                                                                     <  2:2588778/148‑1 (MQ=255)
                                         aGGGCGGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAat                                                                                                     <  2:3320279/148‑1 (MQ=255)
                                          gggCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAata                                                                                                    >  1:2856159/1‑148 (MQ=255)
                                          gggCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAata                                                                                                    <  2:1977727/148‑1 (MQ=255)
                                           ggCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAatat                                                                                                   >  1:800559/1‑148 (MQ=255)
                                           ggCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAatat                                                                                                   >  1:2579081/1‑148 (MQ=255)
                                           ggCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAatat                                                                                                   >  1:1320485/1‑148 (MQ=255)
                                           ggCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAatat                                                                                                   >  1:2190203/1‑148 (MQ=255)
                                           ggCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAatat                                                                                                   <  2:2901392/148‑1 (MQ=255)
                                            gCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATc                                                                                                  <  2:2999078/148‑1 (MQ=255)
                                             cTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATcc                                                                                                 >  2:3377575/1‑148 (MQ=255)
                                               ggCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAg                                                                                               >  2:2967582/1‑148 (MQ=255)
                                                gCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATCTCCAGa                                                                                              >  2:1339299/1‑148 (MQ=255)
                                                  aaTCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACt                                                                                            <  1:2135749/148‑1 (MQ=255)
                                                    tCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCg                                                                                          >  1:1473710/1‑148 (MQ=255)
                                                       ttGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCAt                                                                                       <  1:3816199/148‑1 (MQ=255)
                                                         gCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATcc                                                                                     <  1:2045020/148‑1 (MQ=255)
                                                         gCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATcc                                                                                     <  1:1511841/148‑1 (MQ=255)
                                                           cggcccggcGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCtt                                                                                   <  1:3574667/148‑1 (MQ=255)
                                                                  gcggTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCaaataa                                                                            <  1:908179/148‑1 (MQ=255)
                                                                  gcggTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCaaataa                                                                            <  1:908407/148‑1 (MQ=255)
                                                                   cggTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCaaataaa                                                                           <  2:254645/148‑1 (MQ=255)
                                                                   cggTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCaaataaa                                                                           <  2:1822831/148‑1 (MQ=255)
                                                                    ggTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCaaataaat                                                                          <  1:1369961/148‑1 (MQ=255)
                                                                      tAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCt                                                                        >  1:2421067/1‑148 (MQ=255)
                                                                       aGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTg                                                                       >  1:2688740/1‑148 (MQ=255)
                                                                        gtgtCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGc                                                                      <  2:671631/148‑1 (MQ=255)
                                                                         tgtCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCt                                                                     >  1:1086753/1‑148 (MQ=255)
                                                                         tgtCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCt                                                                     <  2:3465130/148‑1 (MQ=255)
                                                                         tgtCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCt                                                                     >  1:3675236/1‑148 (MQ=255)
                                                                         tgtCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCt                                                                     <  1:246005/148‑1 (MQ=255)
                                                                         tgtCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCt                                                                     >  1:3069877/1‑148 (MQ=255)
                                                                            cTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTgg                                                                  >  2:1621885/1‑148 (MQ=255)
                                                                               tGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTaa                                                               <  1:3377575/148‑1 (MQ=255)
                                                                                   ggCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTc                                                           >  2:1580946/1‑148 (MQ=255)
                                                                                         aGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTaaa                                                     >  2:91928/1‑148 (MQ=255)
                                                                                         aGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTaaa                                                     >  2:3495048/1‑148 (MQ=255)
                                                                                         aGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTaaa                                                     >  1:3185079/1‑148 (MQ=255)
                                                                                          gATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAg                                                    <  2:2190203/148‑1 (MQ=255)
                                                                                          gATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAg                                                    <  1:3238560/148‑1 (MQ=255)
                                                                                            tAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTg                                                  >  2:1507251/1‑148 (MQ=255)
                                                                                            caagaagTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTg                                                  >  2:1507383/2‑148 (MQ=255)
                                                                                             aagaagTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTgg                                                 >  2:1081887/1‑148 (MQ=255)
                                                                                             aagaagTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTgg                                                 <  1:2642296/148‑1 (MQ=255)
                                                                                             aagaagTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTgg                                                 >  2:2055646/1‑148 (MQ=255)
                                                                                               gaagTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCg                                               >  2:870443/1‑148 (MQ=255)
                                                                                               gaagTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCg                                               >  2:870421/1‑148 (MQ=255)
                                                                                                  gTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAAt                                            <  1:1199799/148‑1 (MQ=255)
                                                                                                      cATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAggg                                        <  1:3242608/148‑1 (MQ=255)
                                                                                                       aTCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGt                                       >  1:1818099/1‑148 (MQ=255)
                                                                                                       aTCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGt                                       <  2:2825266/148‑1 (MQ=255)
                                                                                                        tCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGtt                                      >  2:1179097/1‑148 (MQ=255)
                                                                                                         cACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCCCTAGCGGCCACAAACTATTACACACAATATCCAGACTCGCATCCTTCAAATAAATCTGGTTGGTAATGTCCGGAAAGTGGCGGATAGGGGTa                                     >  1:2600499/1‑148 (MQ=255)
                                                                                                           ccTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAAt                                   >  1:1909990/1‑148 (MQ=255)
                                                                                                            cTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATc                                  <  2:3056993/148‑1 (MQ=255)
                                                                                                             ttACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATcc                                 >  2:3697794/1‑148 (MQ=255)
                                                                                                             ttACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATcc                                 >  2:2922224/1‑148 (MQ=255)
                                                                                                              tACGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCa                                >  1:817565/1‑148 (MQ=255)
                                                                                                                cGTCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACg                              <  1:1717762/148‑1 (MQ=255)
                                                                                                                  tCTAAACATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTc                            >  2:1992870/1‑148 (MQ=255)
                                                                                                                     aaaCATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAgg                         >  1:976253/1‑148 (MQ=255)
                                                                                                                     aaaCATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAgg                         >  1:1001104/1‑148 (MQ=255)
                                                                                                                     aaaCATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAgg                         >  2:321206/1‑148 (MQ=255)
                                                                                                                      aaCATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGt                        <  1:3716270/148‑1 (MQ=255)
                                                                                                                       aCATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGtt                       >  1:1142491/1‑148 (MQ=255)
                                                                                                                       aCATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGtt                       <  2:1299238/148‑1 (MQ=255)
                                                                                                                       aCATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGtt                       >  1:2213652/1‑148 (MQ=255)
                                                                                                                       aCATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGtt                       >  2:224682/1‑148 (MQ=255)
                                                                                                                        cATCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGttt                      <  1:1581712/148‑1 (MQ=255)
                                                                                                                         aTCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTTATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGtttt                     >  1:3032289/1‑148 (MQ=255)
                                                                                                                          tCGCGTACGCCGCTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTCATCCACGTCAGGTTTTg                    <  2:1955926/148‑1 (MQ=255)
                                                                                                                                 cgccgcTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCAt             <  2:3744143/148‑1 (MQ=255)
                                                                                                                                     gcTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCg         >  2:1646743/1‑148 (MQ=255)
                                                                                                                                      cTGACCCTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCgg        >  2:3564017/1‑148 (MQ=255)
                                                                                                                                           ccTGTAGTGGCCGTTATCACCCGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGt   >  1:2880125/1‑148 (MQ=255)
                                                                                                                                           ccTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGt   >  1:2879492/1‑148 (MQ=255)
                                                                                                                                           ccTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGt   <  1:1324803/148‑1 (MQ=255)
                                                                                                                                           ccTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGt   <  1:551696/148‑1 (MQ=255)
                                                                                                                                            cTGTAGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGtt  <  2:3256025/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                               
TTGTCCCGCGTTCTGGAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTT  >  CP009273/820359‑820646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑