Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 837,716 G→T 100% intergenic (‑204/‑72) mcbA ← / → rlmF colanic acid mucoidy stimulation protein/23S rRNA m(6)A1618 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273837,7160GT97.6% 672.6 / 6.1 212intergenic (‑204/‑72)mcbA/rlmFcolanic acid mucoidy stimulation protein/23S rRNA m(6)A1618 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base G (1/4);  new base T (120/87);  total (121/91)
Fisher's exact test for biased strand distribution p-value = 1.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 213 total aligned reads displayed.

GCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGATCTCGCCACGCTTTGT  >  CP009273/837572‑837859
                                                                                                                                                |                                                                                                                                               
gCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATtata                                                                                                                                              >  1:724514/1‑148 (MQ=255)
 cAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATaa                                                                                                                                             <  2:3131977/148‑1 (MQ=255)
 cAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATaa                                                                                                                                             <  2:2086193/148‑1 (MQ=255)
   aaTGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAAc                                                                                                                                           >  2:1536099/1‑148 (MQ=255)
     tGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGc                                                                                                                                         >  2:237466/1‑148 (MQ=255)
      gACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCa                                                                                                                                        >  1:2549400/1‑148 (MQ=255)
       aCGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAg                                                                                                                                       <  2:1917509/148‑1 (MQ=255)
            aaaaaTTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGa                                                                                                                                  >  1:2937669/1‑148 (MQ=255)
             aaaaTTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGat                                                                                                                                 <  2:1333207/148‑1 (MQ=255)
             aaaaTTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGat                                                                                                                                 >  2:670920/1‑148 (MQ=255)
               aaTTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGatat                                                                                                                               <  2:2348009/148‑1 (MQ=255)
                  tGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTa                                                                                                                            >  2:176496/1‑148 (MQ=255)
                   gACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTAc                                                                                                                           <  2:2123030/148‑1 (MQ=255)
                    aCAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTAcc                                                                                                                          >  1:2188978/1‑148 (MQ=255)
                           tgtgCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTcgc                                                                                                                   >  1:3737690/1‑148 (MQ=255)
                              gCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGtt                                                                                                                >  2:1522312/1‑148 (MQ=255)
                                 tATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATg                                                                                                             >  1:966336/1‑148 (MQ=255)
                                 tATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATg                                                                                                             >  2:1125137/1‑148 (MQ=255)
                                   ttttttGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCg                                                                                                           <  2:2188150/148‑1 (MQ=255)
                                    tttttGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCgg                                                                                                          >  2:432185/1‑148 (MQ=255)
                                    tttttGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCgg                                                                                                          >  1:1751434/1‑148 (MQ=255)
                                        tGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTAt                                                                                                      <  2:1915144/148‑1 (MQ=255)
                                         gAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATc                                                                                                     >  2:2666421/1‑148 (MQ=255)
                                         gAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATc                                                                                                     <  1:2404715/148‑1 (MQ=255)
                                          aaCGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCt                                                                                                    <  1:2091862/148‑1 (MQ=255)
                                            cGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTa                                                                                                  <  2:742389/148‑1 (MQ=255)
                                              atatatTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCAGGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTAcg                                                                                                >  2:3140706/1‑148 (MQ=255)
                                               tatatTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTAcgc                                                                                               >  2:1038458/1‑148 (MQ=255)
                                               tatatTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTAcgc                                                                                               >  1:1800389/1‑148 (MQ=255)
                                                atatTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTAcgcc                                                                                              >  1:560760/1‑148 (MQ=255)
                                                 tatTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTAcgccg                                                                                             >  1:1709869/1‑148 (MQ=255)
                                                  atTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTAcgccgc                                                                                            >  2:2545443/1‑148 (MQ=255)
                                                     tttACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCgctgc                                                                                         <  1:30883/148‑1 (MQ=255)
                                                     tttACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCgctgc                                                                                         <  2:1849352/148‑1 (MQ=255)
                                                      ttACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCgctgct                                                                                        <  2:2108601/148‑1 (MQ=255)
                                                             aTAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGga                                                                                 >  1:1610888/1‑148 (MQ=255)
                                                              tAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGgag                                                                                <  2:3194543/148‑1 (MQ=255)
                                                              tAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGgag                                                                                >  1:2604778/1‑148 (MQ=255)
                                                               aaGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGgaga                                                                               >  2:3267477/1‑148 (MQ=255)
                                                               aaGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGgaga                                                                               <  1:1780248/148‑1 (MQ=255)
                                                               aaGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGgaga                                                                               <  1:3671846/148‑1 (MQ=255)
                                                                 gACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTCTCTTACGCCGCTGCTTAAAGGAGAAt                                                                             >  2:34195/1‑148 (MQ=255)
                                                                 gACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAAt                                                                             >  1:3498369/1‑148 (MQ=255)
                                                                  aCAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATg                                                                            >  1:3343246/1‑148 (MQ=255)
                                                                  aCAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATg                                                                            >  1:3279891/1‑148 (MQ=255)
                                                                  aCAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGGATCTTACGCCGCTGCTTACAGGAGCATg                                                                            >  1:2287024/1‑148 (MQ=255)
                                                                  aCAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATg                                                                            <  2:847971/148‑1 (MQ=255)
                                                                  aCAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATg                                                                            <  2:2028191/148‑1 (MQ=255)
                                                                   cAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGc                                                                           <  1:1003848/148‑1 (MQ=255)
                                                                       tCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATg                                                                       >  1:3565998/1‑148 (MQ=255)
                                                                       tCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATg                                                                       >  2:130429/1‑148 (MQ=255)
                                                                        cAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGt                                                                      >  2:1193704/1‑148 (MQ=255)
                                                                         aaTGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTc                                                                     <  2:2361857/148‑1 (MQ=255)
                                                                          aTGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTcc                                                                    >  1:3127806/1‑148 (MQ=255)
                                                                          aTGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTcc                                                                    >  2:3444966/1‑148 (MQ=255)
                                                                           tGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCg                                                                   <  1:590407/148‑1 (MQ=255)
                                                                             aCATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGcc                                                                 <  1:278528/148‑1 (MQ=255)
                                                                             aCATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGcc                                                                 <  1:2206068/148‑1 (MQ=255)
                                                                                tGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAg                                                              >  2:2295816/1‑148 (MQ=255)
                                                                                tGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAg                                                              >  1:847583/1‑148 (MQ=255)
                                                                                 gCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGa                                                             >  2:355328/1‑148 (MQ=255)
                                                                                  cATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGaa                                                            <  2:880335/148‑1 (MQ=255)
                                                                                   aTAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGaaa                                                           <  1:1995426/148‑1 (MQ=255)
                                                                                      aTGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCg                                                        <  2:733363/148‑1 (MQ=255)
                                                                                          tGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGt                                                    >  1:47377/1‑148 (MQ=255)
                                                                                             tttttATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCTGGGTTGc                                                 >  1:167416/1‑148 (MQ=255)
                                                                                              ttttATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCa                                                >  2:1017948/1‑148 (MQ=255)
                                                                                              ttttATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCa                                                >  1:1346395/1‑148 (MQ=255)
                                                                                                ttATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATc                                              <  2:247985/148‑1 (MQ=255)
                                                                                                     atgaATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCgcgc                                         >  1:1194321/1‑148 (MQ=255)
                                                                                                      tgaATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCa                                        >  1:1486291/1‑148 (MQ=255)
                                                                                                       gaATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCaa                                       <  1:237466/148‑1 (MQ=255)
                                                                                                       gaATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCaa                                       <  1:1135313/148‑1 (MQ=255)
                                                                                                       gaATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCaa                                       >  2:3585729/1‑148 (MQ=255)
                                                                                                       gaATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCaa                                       <  1:1909442/148‑1 (MQ=255)
                                                                                                       gaATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTCACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCaa                                       >  2:2919197/1‑148 (MQ=255)
                                                                                                          ttGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCg                                    >  1:1433341/1‑148 (MQ=255)
                                                                                                             ccATAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCGGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGtca                                 >  2:2501782/1‑148 (MQ=255)
                                                                                                               aTAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGtcatc                               <  1:3169144/148‑1 (MQ=255)
                                                                                                                tAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGtcatca                              >  1:1770903/1‑148 (MQ=255)
                                                                                                                tAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGtcatca                              <  1:2581481/148‑1 (MQ=255)
                                                                                                                tAAACCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGGTGCATCCGCGCAACCGtcatca                              >  1:768027/1‑148 (MQ=255)
                                                                                                                 aaaCCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATcac                             >  2:537065/1‑148 (MQ=255)
                                                                                                                  aaCCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATcaca                            <  2:3257326/148‑1 (MQ=255)
                                                                                                                   aCCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAg                           <  2:3633841/148‑1 (MQ=255)
                                                                                                                   aCCATAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAg                           <  2:3029966/148‑1 (MQ=255)
                                                                                                                      aTAGCCATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCg                        <  2:1001032/148‑1 (MQ=255)
                                                                                                                           cATGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACg                   <  2:2770491/148‑1 (MQ=255)
                                                                                                                             tGTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGAt                 <  2:448818/148‑1 (MQ=255)
                                                                                                                              gTTAAGGTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGAtc                <  2:3109732/148‑1 (MQ=255)
                                                                                                                                   ggTTTTGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGATCTCGcc           >  1:2562672/1‑148 (MQ=255)
                                                                                                                                     ttttGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGATCTCGCCAc         >  2:158203/1‑148 (MQ=255)
                                                                                                                                     ttttGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGATCTCGCCAc         >  2:158095/1‑148 (MQ=255)
                                                                                                                                        tGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGATCTCGCCACGCt      <  1:425942/148‑1 (MQ=255)
                                                                                                                                        tGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGATCTCGCCACGCt      <  1:2666421/148‑1 (MQ=255)
                                                                                                                                        tGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGATCTCGCCACGCt      <  1:2350334/148‑1 (MQ=255)
                                                                                                                                        tGGGGAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGATCTCGCCACGCt      <  1:1251100/148‑1 (MQ=255)
                                                                                                                                            gAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGATCTCGCCACGCTTTGt  >  1:3620506/1‑148 (MQ=255)
                                                                                                                                            gAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGATCTCGCCACGCTTTGt  >  1:1371142/1‑148 (MQ=255)
                                                                                                                                            gAATTATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGATCTCGCCACGCTTTGt  <  1:1038458/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                               
GCAAATGACGCAAAAAATTGACAGAGATGTGCTTATTTTTTGAACGATATATTTTTACAAAATAAGACAAATCAATGACATGCATAATGCTGATTTTTATGATGAATTGCCATAAACCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCCGCTGCTTAAAGGAGAATGCTATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGATCTCGCCACGCTTTGT  >  CP009273/837572‑837859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑