Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 888,964 C→T 100% intergenic (+75/‑276) ybjN → / → potF negative regulator of motility; multicopy suppressor of coaA(Ts)/putrescine transporter subunit: periplasmic‑binding component of ABC superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273888,9640CT97.7% 717.2 / 5.0 218intergenic (+75/‑276)ybjN/potFnegative regulator of motility; multicopy suppressor of coaA(Ts)/putrescine transporter subunit: periplasmic‑binding component of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base C (3/2);  new base T (100/113);  total (103/115)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 224 total aligned reads displayed.

TGCCCATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATA  >  CP009273/888817‑889107
                                                                                                                                                   |                                                                                                                                               
tGCCCATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTc                                                                                                                                                 <  2:2865110/148‑1 (MQ=255)
     aTCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTaaa                                                                                                                                            >  1:825385/1‑148 (MQ=255)
      tCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAg                                                                                                                                           >  2:155884/1‑148 (MQ=255)
        aaCTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGat                                                                                                                                         <  2:433001/148‑1 (MQ=255)
         aCTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCACAATTATCTGCTGTTTTTAACCTTTTTTTAAAGatt                                                                                                                                        >  2:1925269/1‑148 (MQ=255)
         aCTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGatt                                                                                                                                        >  1:3200737/1‑148 (MQ=255)
          ctgctgTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGatta                                                                                                                                       <  1:1552171/148‑1 (MQ=255)
          ctgctgTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGatta                                                                                                                                       >  2:502590/1‑148 (MQ=255)
            gctgTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGattatt                                                                                                                                     >  2:1666426/1‑148 (MQ=255)
            gctgTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGattatt                                                                                                                                     <  2:2609889/148‑1 (MQ=255)
             ctgTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTAttt                                                                                                                                    <  1:1298046/148‑1 (MQ=255)
               gTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCa                                                                                                                                  <  2:2114242/148‑1 (MQ=255)
                 tACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACt                                                                                                                                >  2:725233/1‑148 (MQ=255)
                 tACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACt                                                                                                                                >  1:1012750/1‑148 (MQ=255)
                  aCCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACtt                                                                                                                               >  1:2997587/1‑148 (MQ=255)
                    cGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTtct                                                                                                                             <  1:2350913/148‑1 (MQ=255)
                     gACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTtctc                                                                                                                            <  2:1844123/148‑1 (MQ=255)
                     gACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTtctc                                                                                                                            <  1:880473/148‑1 (MQ=255)
                      aCTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTctct                                                                                                                           <  1:1315485/148‑1 (MQ=255)
                       cTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCtt                                                                                                                          >  2:2147533/1‑148 (MQ=255)
                         gatgatgaAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTtgt                                                                                                                        <  2:3607639/148‑1 (MQ=255)
                          atgatgaAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTtgtg                                                                                                                       <  2:276840/148‑1 (MQ=255)
                                 aGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGAttt                                                                                                                <  2:2553061/148‑1 (MQ=255)
                                      aaaaCAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCtt                                                                                                           <  2:3249082/148‑1 (MQ=255)
                                        aacaacGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTa                                                                                                         <  1:2728093/148‑1 (MQ=255)
                                         acaacGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTAt                                                                                                        <  2:3579197/148‑1 (MQ=255)
                                          caacGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATc                                                                                                       >  2:1588773/1‑148 (MQ=255)
                                          caacGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATc                                                                                                       >  1:146532/1‑148 (MQ=255)
                                           aacGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTGGATTTGGCTTTATca                                                                                                      >  1:3652650/1‑148 (MQ=255)
                                           aacGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATca                                                                                                      >  1:231305/1‑148 (MQ=255)
                                            acGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATcac                                                                                                     <  1:1957163/148‑1 (MQ=255)
                                             cGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATcaca                                                                                                    <  2:2319101/148‑1 (MQ=255)
                                              gTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACAt                                                                                                   <  1:810821/148‑1 (MQ=255)
                                                gaCCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATag                                                                                                 <  2:2027771/147‑1 (MQ=255)
                                                     aaaaCTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCaa                                                                                            <  1:1113491/148‑1 (MQ=255)
                                                      aaaCTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCaaa                                                                                           >  2:1116937/1‑148 (MQ=255)
                                                      aaaCCATTTCCTCCACGGCTAACTCCCTTCGAGCCCGCAGTCGCTGGTGGAGGGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCATTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCaaa                                                                                           <  2:2919525/148‑1 (MQ=255)
                                                       aaCTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAat                                                                                          <  1:2002049/148‑1 (MQ=255)
                                                       aaCTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAat                                                                                          <  1:3312692/148‑1 (MQ=255)
                                                        aCTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAata                                                                                         <  1:504378/148‑1 (MQ=255)
                                                        aCTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAata                                                                                         <  1:6019/148‑1 (MQ=255)
                                                            tttCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCa                                                                                     >  2:3690164/1‑148 (MQ=255)
                                                            tttCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCa                                                                                     >  1:2417729/1‑148 (MQ=255)
                                                                 tccACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATaaaa                                                                                >  2:2528321/1‑148 (MQ=255)
                                                                 tccACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATaaaa                                                                                <  1:2021448/148‑1 (MQ=255)
                                                                 tccACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATaaaa                                                                                >  2:1837699/1‑148 (MQ=255)
                                                                       gATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGt                                                                          >  1:2464240/1‑148 (MQ=255)
                                                                       gATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGt                                                                          <  2:344942/148‑1 (MQ=255)
                                                                        aTAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGtt                                                                         >  2:2340920/1‑148 (MQ=255)
                                                                        aTAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGtt                                                                         >  2:391907/1‑148 (MQ=255)
                                                                        aTAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGtt                                                                         >  2:1636674/1‑148 (MQ=255)
                                                                           aCTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAGCCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTaaa                                                                      <  1:1196040/148‑1 (MQ=255)
                                                                            cTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAAt                                                                     >  1:1897311/1‑148 (MQ=255)
                                                                            cTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAAt                                                                     >  2:2049867/1‑148 (MQ=255)
                                                                                tttCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCg                                                                 >  2:1261225/1‑148 (MQ=255)
                                                                                tttCGAGCACGCAGTCGCGGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCg                                                                 >  2:3165998/1‑148 (MQ=255)
                                                                                 ttCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTATTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGt                                                                >  2:1519034/1‑148 (MQ=255)
                                                                                  tCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGtt                                                               <  1:1818808/148‑1 (MQ=255)
                                                                                    gAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGtttt                                                             <  2:3238634/148‑1 (MQ=255)
                                                                                      gCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACAGAGAGCAAATATGCATAAAAATTTGTTAAATAACGttattt                                                           >  2:2425237/1‑148 (MQ=255)
                                                                                         cGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAAt                                                        <  1:2503705/148‑1 (MQ=255)
                                                                                         cGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAAt                                                        <  1:3679707/148‑1 (MQ=255)
                                                                                             gTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACAGTTTTTTAATACGa                                                    >  1:2628374/1‑148 (MQ=255)
                                                                                              tCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAg                                                   <  2:1805479/148‑1 (MQ=255)
                                                                                              tCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAg                                                   >  2:3056035/1‑148 (MQ=255)
                                                                                               cGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGc                                                  <  2:2707549/148‑1 (MQ=255)
                                                                                               cGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGc                                                  <  1:1114793/148‑1 (MQ=255)
                                                                                                  tGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCtat                                               <  2:353130/148‑1 (MQ=255)
                                                                                                   ggTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCtata                                              >  1:1426910/1‑148 (MQ=255)
                                                                                                       cAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGtct                                          <  1:3124563/148‑1 (MQ=255)
                                                                                                         gTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCa                                        >  1:933964/1‑148 (MQ=255)
                                                                                                          tGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCaa                                       >  2:2602575/1‑148 (MQ=255)
                                                                                                             cTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAAcc                                    <  1:2289476/148‑1 (MQ=255)
                                                                                                                     tGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTaa                            >  1:3290418/1‑148 (MQ=255)
                                                                                                                       cAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAg                          <  1:3460881/148‑1 (MQ=255)
                                                                                                                        aaaaTTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGt                         <  2:1420798/148‑1 (MQ=255)
                                                                                                                        aaaaTTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGt                         <  1:1226514/148‑1 (MQ=255)
                                                                                                                          aaTTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTa                       >  2:2989302/1‑148 (MQ=255)
                                                                                                                             tATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTc                    >  1:2129165/1‑148 (MQ=255)
                                                                                                                              aTCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCt                   <  2:642155/148‑1 (MQ=255)
                                                                                                                               tCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCtt                  >  1:673046/1‑148 (MQ=255)
                                                                                                                                ctgctgTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTg                 >  2:2302598/1‑148 (MQ=255)
                                                                                                                                  gctgTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCg               <  2:3356688/148‑1 (MQ=255)
                                                                                                                                   ctgTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGa              <  2:1156241/148‑1 (MQ=255)
                                                                                                                                    tgTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAATCCCTGGCTAAAGTTATTCTTGCGAt             >  1:3235757/1‑148 (MQ=255)
                                                                                                                                    tgTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGAt             >  2:2838704/1‑148 (MQ=255)
                                                                                                                                      tttttAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGc           <  2:1077502/148‑1 (MQ=255)
                                                                                                                                      tttttAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGc           >  1:455128/1‑148 (MQ=255)
                                                                                                                                      tttttAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGc           <  2:3200737/148‑1 (MQ=255)
                                                                                                                                       ttttAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCt          <  2:1045855/148‑1 (MQ=255)
                                                                                                                                          tAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCtttt       <  1:2147533/148‑1 (MQ=255)
                                                                                                                                           aaCCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTta      <  1:1614275/148‑1 (MQ=255)
                                                                                                                                            aCCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTtat     <  1:3553313/148‑1 (MQ=255)
                                                                                                                                            aCCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTtat     >  2:2403229/1‑148 (MQ=255)
                                                                                                                                            aCCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTtat     <  2:825385/148‑1 (MQ=255)
                                                                                                                                            aCCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCTTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTtat     >  1:1986572/1‑148 (MQ=255)
                                                                                                                                             ccTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTtata    <  2:3646415/148‑1 (MQ=255)
                                                                                                                                              cTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTtatat   >  2:3339177/1‑148 (MQ=255)
                                                                                                                                              cTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTtatat   <  2:2448338/148‑1 (MQ=255)
                                                                                                                                               ttttttTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTtatata  >  2:2026187/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
TGCCCATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATA  >  CP009273/888817‑889107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑