Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 943,573 G→T 100% A297S (GCC→TCC)  ycaM → putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273943,5730GT100.0% 901.7 / NA 269A297S (GCC→TCC) ycaMputative transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (146/123);  total (146/123)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 269 total aligned reads displayed.

TTGTGCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGTTAATGGCT  >  CP009273/943428‑943717
                                                                                                                                                 |                                                                                                                                                
ttGTGCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTcc                                                                                                                                                >  2:2013147/1‑148 (MQ=255)
 tgtgCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCa                                                                                                                                               <  1:3167489/148‑1 (MQ=255)
  gtgCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCAt                                                                                                                                              <  1:1888281/148‑1 (MQ=255)
    gCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTg                                                                                                                                            <  2:975084/148‑1 (MQ=255)
     ccATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGc                                                                                                                                           >  1:1600359/1‑148 (MQ=255)
       aTTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTgaga                                                                                                                                         >  2:2132623/1‑148 (MQ=255)
       aTTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGa                                                                                                                                         <  1:3350795/148‑1 (MQ=255)
       aTTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGa                                                                                                                                         <  1:1449822/148‑1 (MQ=255)
        ttCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGaa                                                                                                                                        >  2:3068962/1‑148 (MQ=255)
                     ggCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACaa                                                                                                                           <  2:1101341/148‑1 (MQ=255)
                     ggCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCAGGGACaa                                                                                                                           >  2:920232/1‑148 (MQ=255)
                       cGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGt                                                                                                                         <  2:2333871/148‑1 (MQ=255)
                             ggATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAgcggc                                                                                                                   >  1:308586/1‑148 (MQ=255)
                              gatgatGTTTGATTCGCTTAATATCCCGGATGACTTAATGACCAAGGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTATTGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAgcggcg                                                                                                                  <  1:881045/148‑1 (MQ=255)
                               atgatgTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGgcgc                                                                                                                 <  1:3796200/148‑1 (MQ=255)
                                 gatgTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTg                                                                                                               >  2:2056516/1‑148 (MQ=255)
                                      ttGATTGGAGTATTACCCGGGTTGACTTAATCACAAACTGTCATTATTACGCCTTTCTGAAGCGGGGCGTGTATTACAACATGGGTAATACTTTATTGGTGTTTTACTCCATTGCGAATACCCTGGGAAAAGTAGCGGCGCTGGTAtt                                                                                                          <  1:3633769/148‑1 (MQ=255)
                                      ttGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTAtt                                                                                                          <  2:379932/148‑1 (MQ=255)
                                          ttCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCg                                                                                                      >  2:1469433/1‑148 (MQ=255)
                                           tCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGa                                                                                                     <  2:2943294/148‑1 (MQ=255)
                                             gcttAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGAtt                                                                                                   <  1:1333868/145‑1 (MQ=255)
                                              cgTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTg                                                                                                  <  2:1039854/148‑1 (MQ=255)
                                                 aaTATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATg                                                                                               >  2:899088/1‑148 (MQ=255)
                                                 aaTATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATg                                                                                               >  1:2850711/1‑148 (MQ=255)
                                                    atCCCGGATGACTTAATGACCACCGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGccc                                                                                            <  1:2969469/148‑1 (MQ=255)
                                                    atCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGccc                                                                                            >  2:1713966/1‑148 (MQ=255)
                                                      cccGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCg                                                                                          >  1:710419/1‑148 (MQ=255)
                                                      cccGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCg                                                                                          <  2:247397/148‑1 (MQ=255)
                                                       ccGGATGACTTAATTACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTAACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGc                                                                                         <  2:2579092/148‑1 (MQ=255)
                                                            tGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTaaa                                                                                    >  1:2127477/1‑148 (MQ=255)
                                                             gACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAg                                                                                   <  2:3204911/148‑1 (MQ=255)
                                                               cTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTg                                                                                 <  2:3701753/148‑1 (MQ=255)
                                                                ttAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGc                                                                                >  2:1997570/1‑148 (MQ=255)
                                                                 tAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCt                                                                               >  1:734754/1‑148 (MQ=255)
                                                                    tGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTAtt                                                                            >  1:2435457/1‑148 (MQ=255)
                                                                    tGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTAtt                                                                            >  1:2389695/1‑148 (MQ=255)
                                                                    tGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTAtt                                                                            >  1:2389123/1‑148 (MQ=255)
                                                                      aCCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTGAAAGTGCTATTAg                                                                          >  2:436291/1‑148 (MQ=255)
                                                                       ccAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAgg                                                                         <  2:1197765/148‑1 (MQ=255)
                                                                        cAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGt                                                                        >  2:647113/1‑148 (MQ=255)
                                                                        cAACGGTCAGTATTACGCCTTTCAGAAGCGGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGt                                                                        <  1:2911831/148‑1 (MQ=255)
                                                                           cGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGAt                                                                     >  1:1065289/1‑148 (MQ=255)
                                                                            ggTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATg                                                                    <  1:1112232/148‑1 (MQ=255)
                                                                             gTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGc                                                                   <  2:2850960/148‑1 (MQ=255)
                                                                             gTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGc                                                                   <  1:2645310/148‑1 (MQ=255)
                                                                             gTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGc                                                                   <  1:2977322/148‑1 (MQ=255)
                                                                             gTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGc                                                                   <  2:1404404/148‑1 (MQ=255)
                                                                              tCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCt                                                                  >  2:2779227/1‑148 (MQ=255)
                                                                               cAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTg                                                                 <  2:1885759/148‑1 (MQ=255)
                                                                               cAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTg                                                                 <  1:2941844/148‑1 (MQ=255)
                                                                                aGTATTACGCCTTTCCGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGa                                                                <  1:2050992/148‑1 (MQ=255)
                                                                                  tATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACa                                                              >  2:1821640/1‑148 (MQ=255)
                                                                                    ttACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGc                                                            >  2:3429244/1‑148 (MQ=255)
                                                                                      aCGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCaa                                                          <  1:1462717/148‑1 (MQ=255)
                                                                                      aCGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCaa                                                          <  2:3169955/148‑1 (MQ=255)
                                                                                       cGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCaca                                                         >  2:1275644/1‑146 (MQ=255)
                                                                                       cGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCaaa                                                         <  1:2527991/148‑1 (MQ=255)
                                                                                        gCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAat                                                        <  2:327351/148‑1 (MQ=255)
                                                                                        gCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAat                                                        <  1:2132509/148‑1 (MQ=255)
                                                                                          cTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAatat                                                      <  2:3080535/148‑1 (MQ=255)
                                                                                          cTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAatat                                                      >  2:2525375/1‑148 (MQ=255)
                                                                                            ttCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAatatat                                                    <  1:1498285/148‑1 (MQ=255)
                                                                                             tCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATAtt                                                   <  2:825693/148‑1 (MQ=255)
                                                                                             tCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATAtt                                                   <  1:1990980/148‑1 (MQ=255)
                                                                                               aGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCGTAAAGTGGGATTAGGTGATGCTGACAGCCAATATATTcc                                                 >  1:361850/1‑148 (MQ=255)
                                                                                                gAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTcca                                                <  2:1529366/148‑1 (MQ=255)
                                                                                                gAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTcca                                                >  1:793713/1‑148 (MQ=255)
                                                                                                 aaGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTccag                                               >  2:2968285/1‑148 (MQ=255)
                                                                                                 aaGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTccag                                               >  2:3331615/1‑148 (MQ=255)
                                                                                                   gCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTccagcc                                             <  2:3371279/148‑1 (MQ=255)
                                                                                                    cTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTccagcca                                            >  2:3478079/1‑148 (MQ=255)
                                                                                                       ggCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCGGtt                                         >  1:3106229/1‑148 (MQ=255)
                                                                                                        gCGAGTATTACAACATGGGTAATAATTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGttt                                        <  1:1749888/148‑1 (MQ=255)
                                                                                                         cGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTa                                       <  2:2675974/148‑1 (MQ=255)
                                                                                                            gTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGt                                    <  2:1126338/148‑1 (MQ=255)
                                                                                                                tACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTa                                >  1:1740354/1‑148 (MQ=255)
                                                                                                                tACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTa                                >  1:2792650/1‑148 (MQ=255)
                                                                                                                tACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTa                                >  2:2906225/1‑148 (MQ=255)
                                                                                                                tACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTa                                >  2:1634220/1‑148 (MQ=255)
                                                                                                                tACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTa                                >  2:1629202/1‑148 (MQ=255)
                                                                                                                 acaacaTGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCAAGCCAGTTTATGTCGTAc                               >  2:3448964/1‑148 (MQ=255)
                                                                                                                      aTGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACg                          >  1:1177413/1‑148 (MQ=255)
                                                                                                                      aTGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACg                          <  2:1582435/148‑1 (MQ=255)
                                                                                                                        gggTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCt                        <  1:1273602/148‑1 (MQ=255)
                                                                                                                         ggTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCtt                       <  2:3561530/148‑1 (MQ=255)
                                                                                                                         ggTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCtt                       >  1:567782/1‑148 (MQ=255)
                                                                                                                           tAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCt                     >  2:2713160/1‑148 (MQ=255)
                                                                                                                            aaTACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTg                    >  1:3469958/1‑148 (MQ=255)
                                                                                                                             aTACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTgg                   >  1:421539/1‑148 (MQ=255)
                                                                                                                               aCTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTa                 >  1:1896289/1‑148 (MQ=255)
                                                                                                                                    aaTGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGccc            >  1:268798/1‑148 (MQ=255)
                                                                                                                                     aTGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTTACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCGTCTGGTACGCCCg           >  1:2293319/1‑148 (MQ=255)
                                                                                                                                       ggTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGtt         >  2:2043275/1‑148 (MQ=255)
                                                                                                                                         tGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGTTaa       <  2:2152382/148‑1 (MQ=255)
                                                                                                                                         tGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGTTaa       >  2:1235471/1‑148 (MQ=255)
                                                                                                                                          gATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGt                                    <  1:3235256/118‑1 (MQ=255)
                                                                                                                                          gATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGTTAAt      >  1:2110068/1‑148 (MQ=255)
                                                                                                                                          gATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGTTAAt      >  1:2457962/1‑148 (MQ=255)
                                                                                                                                            tttACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGTTAATgg    <  1:447312/148‑1 (MQ=255)
                                                                                                                                              tACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGTTAATGGCt  <  2:2518761/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TTGTGCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGTTAATGGCT  >  CP009273/943428‑943717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑