BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,031,861	G→A	6.7%	G18S (GGC→AGC)	hyaE →	putative HyaA chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,031,861	0	G	A	6.7%	634.6 / 20.5	178	G18S (GGC→AGC)	hyaE	putative HyaA chaperone
Reads supporting (aligned to +/- strand): ref base G (94/72); new base A (5/7); total (99/79)
Fisher's exact test for biased strand distribution p-value = 3.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 185 total aligned reads displayed.

AATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCG  >  CP009273/1031715‑1032008

 aTGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGc                                                                                                                                                   >  2:494360/1‑148 (MQ=255)

  tGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGAAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCt                                                                                                                                                  <  1:3723751/148‑1 (MQ=255)

   gAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTg                                                                                                                                                 >  2:2376128/1‑148 (MQ=255)

   gAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTg                                                                                                                                                 >  1:2456045/1‑148 (MQ=255)

    aaTCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTgg                                                                                                                                                >  1:3637890/1‑148 (MQ=255)

      tCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGAc                                                                                                                                              >  2:763805/1‑148 (MQ=255)

        gcgcTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGc                                                                                                                                            <  2:1966595/148‑1 (MQ=255)

            tGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCcagt                                                                                                                                        <  2:3416896/148‑1 (MQ=255)

            tGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCcagt                                                                                                                                        <  1:183458/148‑1 (MQ=255)

            tGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCAGCTGGACGCcagt                                                                                                                                        <  2:1822357/148‑1 (MQ=255)

               ctcAATTATGACTGTCTGTCGATGGAAAAGTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCcagtcag                                                                                                                                     <  2:3236722/148‑1 (MQ=255)

                    ttATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAAt                                                                                                                                >  2:1047542/1‑148 (MQ=255)

                        gACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCg                                                                                                                            >  2:3487433/1‑148 (MQ=255)

                        gACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCg                                                                                                                            >  2:2111255/1‑148 (MQ=255)

                         aCTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGt                                                                                                                           <  2:2895186/148‑1 (MQ=255)

                          ctgtcggtCGATGGAAAATTACGAACGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGGTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTc                                                                                                                          <  1:3007073/148‑1 (MQ=255)

                           tgtctgtcGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACTCCAGTCAGTGAATCCCGTCt                                                                                                                         <  1:1426176/148‑1 (MQ=255)

                            gtctgtcGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCtt                                                                                                                        >  2:1547205/1‑148 (MQ=255)

                              ctgtcGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTga                                                                                                                      >  1:461716/1‑148 (MQ=255)

                                gtcGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCAGCTGGACGCCAGTCAGTGAATCCCGTCTTgacg                                                                                                                    >  2:2508052/1‑148 (MQ=255)

                                      ggAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGc                                                                                                              >  1:1554663/1‑148 (MQ=255)

                                      ggAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGc                                                                                                              >  1:3158701/1‑148 (MQ=255)

                                       gAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCt                                                                                                             >  2:345902/1‑148 (MQ=255)

                                       gAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACCGGCt                                                                                                             >  2:872303/1‑148 (MQ=255)

                                        aaaaTTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCtt                                                                                                            >  2:3326100/1‑148 (MQ=255)

                                           aTTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACg                                                                                                         >  1:1621039/1‑148 (MQ=255)

                                           aTTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACg                                                                                                         >  1:1620968/1‑148 (MQ=255)

                                            ttACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGc                                                                                                        >  2:2656170/1‑148 (MQ=255)

                                            ttACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGc                                                                                                        >  1:1551532/1‑148 (MQ=255)

                                            ttACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGc                                                                                                        >  2:1532743/1‑148 (MQ=255)

                                             tACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCa                                                                                                       <  2:1515279/148‑1 (MQ=255)

                                                 aaGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAgcg                                                                                                   >  1:2371666/1‑148 (MQ=255)

                                                 aaGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAgcg                                                                                                   >  2:511781/1‑148 (MQ=255)

                                                       ttCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGAc                                                                                             >  2:235191/1‑148 (MQ=255)

                                                        tCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACg                                                                                            >  1:1766386/1‑148 (MQ=255)

                                                        tCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACg                                                                                            >  1:3717783/1‑148 (MQ=255)

                                                           cgtGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCg                                                                                         <  2:2795283/146‑1 (MQ=255)

                                                           cTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCg                                                                                         <  1:3648215/148‑1 (MQ=255)

                                                           cTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCg                                                                                         <  1:3097348/148‑1 (MQ=255)

                                                             tGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCgtg                                                                                       >  1:2593324/1‑148 (MQ=255)

                                                             tGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCgtg                                                                                       >  1:2668912/1‑148 (MQ=255)

                                                              gcgcCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCAGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCgtgg                                                                                      <  1:2508052/148‑1 (MQ=255)

                                                                gcCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCgtggtg                                                                                    <  1:1582872/148‑1 (MQ=255)

                                                                  cAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGtt                                                                                  <  1:2795488/148‑1 (MQ=255)

                                                                    gTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGGTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGttat                                                                                >  2:3030754/1‑148 (MQ=255)

                                                                     tACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCAGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGttatt                                                                               >  1:542039/1‑148 (MQ=255)

                                                                       ccGGATTACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCAGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTaa                                                                             >  1:2081327/1‑148 (MQ=255)

                                                                       ccGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTaa                                                                             <  2:3320430/148‑1 (MQ=255)

                                                                          gATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCa                                                                          >  1:2296431/1‑148 (MQ=255)

                                                                            tGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGt                                                                        >  2:1078271/1‑148 (MQ=255)

                                                                                acaGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGAcc                                                                    <  2:2258987/148‑1 (MQ=255)

                                                                                acaGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGAcc                                                                    <  2:1332761/148‑1 (MQ=255)

                                                                                acaGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGAcc                                                                    <  2:1387213/148‑1 (MQ=255)

                                                                                  aggaggAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCg                                                                  >  2:3471761/1‑148 (MQ=255)

                                                                                    gaggagCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGaa                                                                <  2:808668/148‑1 (MQ=255)

                                                                                    gaggagCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGaa                                                                >  2:401645/1‑148 (MQ=255)

                                                                                       gagCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAacg                                                             >  1:637632/1‑148 (MQ=255)

                                                                                             ggATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGcc                                                       >  1:883874/1‑148 (MQ=255)

                                                                                              gATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCa                                                      <  1:308868/148‑1 (MQ=255)

                                                                                               aTGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGACTGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCag                                                     <  1:1180679/148‑1 (MQ=255)

                                                                                               aTGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCag                                                     >  2:599290/1‑148 (MQ=255)

                                                                                                  aGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAgg                                                  <  1:2376156/148‑1 (MQ=255)

                                                                                                      aCGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAg                                              <  2:3637890/148‑1 (MQ=255)

                                                                                                         acacGCCATTTGATGCGTTTTGGCTACTAATGCTGGCGCGCGGGTGGACGCCTGTCTGTGTAGCCCGTCTTGACGAGTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTATGCATTGACCCGAAACGCACGCCAGATGTCAGCGa                                           <  2:1776626/148‑1 (MQ=255)

                                                                                                         acacGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGa                                           >  1:941294/1‑148 (MQ=255)

                                                                                                         acacGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGa                                           >  1:54685/1‑148 (MQ=255)

                                                                                                         acacGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGa                                           >  1:933236/1‑148 (MQ=255)

                                                                                                         acacGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGa                                           >  1:941300/1‑148 (MQ=255)

                                                                                                         acacGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGa                                           <  2:1446027/148‑1 (MQ=255)

                                                                                                           acGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCAGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATa                                         >  1:3523377/1‑148 (MQ=255)

                                                                                                            cGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATaa                                        >  1:1275997/1‑148 (MQ=255)

                                                                                                             gCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAAt                                       <  1:537228/148‑1 (MQ=255)

                                                                                                                aTTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCg                                    >  2:2096952/1‑148 (MQ=255)

                                                                                                                aTTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCg                                    >  2:2031727/1‑148 (MQ=255)

                                                                                                                    gATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTaa                                <  1:3326111/148‑1 (MQ=255)

                                                                                                                    gATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTaa                                <  1:3326093/148‑1 (MQ=255)

                                                                                                                     aTGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAAt                               >  2:1121463/1‑148 (MQ=255)

                                                                                                                      tGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATg                              <  2:2716125/148‑1 (MQ=255)

                                                                                                                        cGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGAt                            >  1:77187/1‑148 (MQ=255)

                                                                                                                        cGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGAt                            <  2:382599/148‑1 (MQ=255)

                                                                                                                           tgggccAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCTTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTgg                         <  1:3632394/143‑1 (MQ=255)

                                                                                                                              ggCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGa                      <  2:1565775/148‑1 (MQ=255)

                                                                                                                              ggCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGa                      >  1:1024919/1‑148 (MQ=255)

                                                                                                                                cAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAAt                    <  1:3684028/148‑1 (MQ=255)

                                                                                                                                cAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAAt                    >  2:3403465/1‑148 (MQ=255)

                                                                                                                                cAACGAATGCTGGCGCGCAGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAAt                    <  2:1696402/148‑1 (MQ=255)

                                                                                                                                 aaCGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAAtt                   <  2:1791707/148‑1 (MQ=255)

                                                                                                                                 aaCGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAAtt                   <  2:489746/148‑1 (MQ=255)

                                                                                                                                 aaCGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAAtt                   <  2:1791759/148‑1 (MQ=255)

                                                                                                                                  aCGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTa                  >  1:2184910/1‑148 (MQ=255)

                                                                                                                                   cGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTAc                 >  2:3413437/1‑148 (MQ=255)

                                                                                                                                   cGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTAc                 >  2:3228487/1‑148 (MQ=255)

                                                                                                                                   cGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTAc                 >  1:1327148/1‑148 (MQ=255)

                                                                                                                                     aaTGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTg               <  2:1094245/148‑1 (MQ=255)

                                                                                                                                       tGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTgcg             >  2:222106/1‑148 (MQ=255)

                                                                                                                                            gcgcgcGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGt        >  1:2897976/1‑148 (MQ=255)

                                                                                                                                             cgcgcgGCTGGACGCAAGTCATTGAATCCCTTCTTGACTACTGTCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGtt       <  1:345902/148‑1 (MQ=255)

                                                                                                                                               cgcgGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTc     <  2:2388924/148‑1 (MQ=255)

                                                                                                                                               cgcgGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTc     <  1:1547183/148‑1 (MQ=255)

                                                                                                                                                  ggCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCg  <  2:2469832/148‑1 (MQ=255)

AATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCG  >  CP009273/1031715‑1032008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`