Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,083,443 G→T 100% S624* (TCG→TAG)  pgaB ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine (PGA) N‑deacetylase outer membrane export lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,083,4430GT99.2% 874.3 / ‑2.1 265S624* (TCG→TAG) pgaBpoly‑beta‑1,6‑N‑acetyl‑D‑glucosamine (PGA) N‑deacetylase outer membrane export lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (128/135);  total (129/136)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 275 total aligned reads displayed.

ATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCC  >  CP009273/1083298‑1083589
                                                                                                                                                 |                                                                                                                                                  
aTCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCtaa                                                                                                                                                  >  1:2902707/1‑148 (MQ=255)
 tCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCtaag                                                                                                                                                 >  1:2017202/1‑148 (MQ=255)
  cATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCtaaga                                                                                                                                                >  2:3427548/1‑148 (MQ=255)
   aTTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCtaagaa                                                                                                                                               <  2:2377740/148‑1 (MQ=255)
   aTTTTTCGGAGACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCtaagaa                                                                                                                                               <  2:3590398/148‑1 (MQ=255)
     ttttCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAAt                                                                                                                                             <  2:827588/148‑1 (MQ=255)
     ttttCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAAt                                                                                                                                             <  2:2959517/148‑1 (MQ=255)
     ttttCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAAt                                                                                                                                             >  2:1088162/1‑148 (MQ=255)
      tttCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATa                                                                                                                                            <  2:1325080/148‑1 (MQ=255)
        tCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGc                                                                                                                                          <  2:1895667/148‑1 (MQ=255)
        tCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGc                                                                                                                                          <  1:2074327/148‑1 (MQ=255)
           gATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTg                                                                                                                                       >  1:2747218/1‑148 (MQ=255)
           gATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTg                                                                                                                                       >  1:2440163/1‑148 (MQ=255)
            aTACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGa                                                                                                                                      <  2:3302530/148‑1 (MQ=255)
            aTACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGa                                                                                                                                      <  2:637605/148‑1 (MQ=255)
              aCCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATg                                                                                                                                    <  2:890569/148‑1 (MQ=255)
              aCCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATg                                                                                                                                    >  1:836533/1‑148 (MQ=255)
              aCCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATg                                                                                                                                    <  1:2695235/148‑1 (MQ=255)
              aCCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATg                                                                                                                                    >  1:1031287/1‑148 (MQ=255)
                cAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCt                                                                                                                                  <  1:3188888/148‑1 (MQ=255)
                   gCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGAc                                                                                                                               >  2:3291504/1‑148 (MQ=255)
                       ttGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCAtt                                                                                                                           >  1:3358569/1‑148 (MQ=255)
                          aaaaCTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCAttttt                                                                                                                        <  2:1949623/148‑1 (MQ=255)
                          aaaaCTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCAttttt                                                                                                                        >  1:3296148/1‑148 (MQ=255)
                            aaCTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCt                                                                                                                      >  2:315956/1‑148 (MQ=255)
                            aaCTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGATGACCATTTTTCt                                                                                                                      >  1:652959/1‑148 (MQ=255)
                             aCTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTg                                                                                                                     >  2:2878384/1‑148 (MQ=255)
                             aCTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTg                                                                                                                     >  2:1513194/1‑148 (MQ=255)
                                  ggACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGt                                                                                                                <  1:1235010/148‑1 (MQ=255)
                                        aTAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTtgtg                                                                                                          >  1:915466/1‑148 (MQ=255)
                                         tAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGc                                                                                                         >  2:742629/1‑148 (MQ=255)
                                         tAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGc                                                                                                         >  2:279569/1‑148 (MQ=255)
                                          aaGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGcc                                                                                                        >  2:1409978/1‑148 (MQ=255)
                                             gTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGt                                                                                                     >  2:1024554/1‑148 (MQ=255)
                                              tCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTa                                                                                                    >  2:1804011/1‑148 (MQ=255)
                                               cTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTaa                                                                                                   >  1:1501203/1‑148 (MQ=255)
                                                   ttCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCt                                                                                               >  2:2824432/1‑148 (MQ=255)
                                                        gttggttATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAAt                                                                                          <  1:3575834/148‑1 (MQ=255)
                                                          tggttATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAg                                                                                        <  2:2654988/148‑1 (MQ=255)
                                                          tggttATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAg                                                                                        <  2:14541/148‑1 (MQ=255)
                                                           ggttATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGa                                                                                       <  2:2350618/148‑1 (MQ=255)
                                                            gttATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGAt                                                                                      <  1:958386/148‑1 (MQ=255)
                                                             ttATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGGTTTGTGCCTGTAATTCTAAAATAGAtt                                                                                     >  2:3331929/1‑148 (MQ=255)
                                                                 gCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTc                                                                                 <  2:3099577/148‑1 (MQ=255)
                                                                 gCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTc                                                                                 <  2:3218845/148‑1 (MQ=255)
                                                                 gCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTc                                                                                 <  1:1628493/148‑1 (MQ=255)
                                                                    gAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCttt                                                                              <  2:3607371/148‑1 (MQ=255)
                                                                    gAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCttt                                                                              <  1:3455493/148‑1 (MQ=255)
                                                                    gAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCttt                                                                              >  2:2260402/1‑148 (MQ=255)
                                                                     aaaaTTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTa                                                                             >  1:952479/1‑148 (MQ=255)
                                                                     aaaaTTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTa                                                                             >  1:3063064/1‑148 (MQ=255)
                                                                     aaaaTTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTa                                                                             >  1:2373799/1‑148 (MQ=255)
                                                                       aaTTGTCGGGATAATACCCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGc                                                                           <  2:2178273/148‑1 (MQ=255)
                                                                       aaTTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGc                                                                           <  2:3371585/148‑1 (MQ=255)
                                                                        aTTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGcc                                                                          <  1:1713371/148‑1 (MQ=255)
                                                                         ttGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCt                                                                         >  2:2881218/1‑148 (MQ=255)
                                                                          tGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTg                                                                        >  2:1692454/1‑148 (MQ=255)
                                                                          tGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTg                                                                        >  1:1246101/1‑148 (MQ=255)
                                                                          tGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTg                                                                        <  2:208302/148‑1 (MQ=255)
                                                                               ggATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGa                                                                   >  1:601484/1‑148 (MQ=255)
                                                                                gATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGAt                                                                  >  2:3625214/1‑148 (MQ=255)
                                                                                   aataaCCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGtt                                                               <  2:1728126/148‑1 (MQ=255)
                                                                                    ataaCCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGttt                                                              <  2:1592762/148‑1 (MQ=255)
                                                                                    ataaCCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGttt                                                              <  1:2280294/148‑1 (MQ=255)
                                                                                     taaCCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACAATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGtttt                                                             >  1:133187/1‑148 (MQ=255)
                                                                                         cATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAAt                                                         <  1:765709/148‑1 (MQ=255)
                                                                                             gTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGa                                                     <  1:3053314/148‑1 (MQ=255)
                                                                                               ttttCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGAtt                                                   >  2:1559504/1‑148 (MQ=255)
                                                                                                tttCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTg                                                  <  1:1496561/148‑1 (MQ=255)
                                                                                                  tCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGt                                                <  1:1888594/148‑1 (MQ=255)
                                                                                                  tCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGt                                                >  1:156736/1‑148 (MQ=255)
                                                                                                    aCTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCa                                              >  1:1172402/1‑148 (MQ=255)
                                                                                                    aCTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCa                                              >  1:1859454/1‑148 (MQ=255)
                                                                                                     cTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCaa                                             <  2:3507052/148‑1 (MQ=255)
                                                                                                      tCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAAt                                            <  2:837940/148‑1 (MQ=255)
                                                                                                      tCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAAt                                            >  1:2214610/1‑148 (MQ=255)
                                                                                                      tCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAAt                                            >  2:410397/1‑148 (MQ=255)
                                                                                                       ccATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAAtt                                           <  2:1172402/148‑1 (MQ=255)
                                                                                                        cATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTg                                          <  1:1696242/148‑1 (MQ=255)
                                                                                                         aTTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGt                                         <  2:3389315/148‑1 (MQ=255)
                                                                                                          ttCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTa                                        <  2:3250594/148‑1 (MQ=255)
                                                                                                          ttCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTa                                        <  1:742629/148‑1 (MQ=255)
                                                                                                             aGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTa                                     <  2:2514621/148‑1 (MQ=255)
                                                                                                              gTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGTCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTaa                                    <  1:2681986/148‑1 (MQ=255)
                                                                                                                  tAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCAt                                <  1:1284877/148‑1 (MQ=255)
                                                                                                                  tAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCAt                                <  1:1199750/148‑1 (MQ=255)
                                                                                                                      aGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGt                            <  1:459140/148‑1 (MQ=255)
                                                                                                                      aGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGt                            <  2:1575055/148‑1 (MQ=255)
                                                                                                                      aGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGt                            <  1:1411399/148‑1 (MQ=255)
                                                                                                                          tCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGc                        <  2:1278446/148‑1 (MQ=255)
                                                                                                                            aTCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCg                      <  2:555247/148‑1 (MQ=255)
                                                                                                                              ccAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGAt                    >  2:3455679/1‑148 (MQ=255)
                                                                                                                              ccAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGAt                    <  2:2768194/148‑1 (MQ=255)
                                                                                                                                 gTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGAtttt                 >  1:3018378/1‑148 (MQ=255)
                                                                                                                                 gTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGAtttt                 >  1:3018421/1‑148 (MQ=255)
                                                                                                                                      cGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGc            <  2:3557008/148‑1 (MQ=255)
                                                                                                                                        aGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGa          >  1:1918033/1‑148 (MQ=255)
                                                                                                                                          ttgttgCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGaca        <  1:1972670/148‑1 (MQ=255)
                                                                                                                                          ttgttgCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGaca        >  2:685909/1‑148 (MQ=255)
                                                                                                                                                cGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTca  >  2:3699692/1‑147 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
ATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCC  >  CP009273/1083298‑1083589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑