Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,118,542 C→G 7.3% S63T (AGC→ACC)  yceB ← lipoprotein, DUF1439 family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,118,5420CG 7.3% 490.9 / 2.1 169S63T (AGC→ACC) yceBlipoprotein, DUF1439 family
Reads supporting (aligned to +/- strand):  ref base C (63/90);  new base G (2/12);  total (66/103)
Fisher's exact test for biased strand distribution p-value = 8.23e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 172 total aligned reads displayed.

AAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGG  >  CP009273/1118395‑1118688
                                                                                                                                                    |                                                                                                                                                   
aaGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTATGACTACCGAACAGGGCGTTCATGTACAGATTGGGGTCTCCGGGTAGGGTAACCATATTAGGCTCTTCGCGACCAAATT‑TGc                                                                                                                                                     >  1:1774684/1‑148 (MQ=255)
 aGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTGTGACTACCGAACAGGGAGTTCATGTCCCGATTTGGGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAAATT‑TGCt                                                                                                                                                    >  1:3625626/1‑148 (MQ=255)
    tCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTGTTTCTAACGAACAGGGAGTTGATTTGCAGATTTGGGTGTCCGGTTAGGGTTAACTTATTAGGCTCGTCGCGACCAATTT‑Tggtggt                                                                                                                                                 >  1:1956432/1‑148 (MQ=255)
    tCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGt                                                                                                                                                 <  1:2114270/148‑1 (MQ=255)
        aCCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAgg                                                                                                                                            >  2:514042/1‑148 (MQ=255)
        aCCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAgg                                                                                                                                            >  2:3601624/1‑148 (MQ=255)
        aCCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAgg                                                                                                                                            >  2:2435661/1‑148 (MQ=255)
        aCCTTTTACTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTATCTTACTAACGAAAACGGATTGAAAGTACAGAATGGCGTCACCGGGTAGGGTAACCTTATTCGGCAAGTAGCGACCAAATT‑GGCTGGT‑CAgg                                                                                                                                            >  2:1676827/1‑148 (MQ=255)
         ccTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCAAGTCCAGATTGGCGTCACCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAAATT‑GGCTGGT‑CAGGt                                                                                                                                           >  2:2182108/1‑148 (MQ=255)
            ttttCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGCGTTGATGTCCAGATTGGGGGCTCCGGGTAGGGGAACCGTATTAGGCTCTTCGCGACCAATAT‑GGGTGGT‑CAGGTTTg                                                                                                                                        >  1:2323443/1‑148 (MQ=255)
             tttCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGt                                                                                                                                       <  1:517138/148‑1 (MQ=255)
              ttCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTGTGAATACCGAACAGGGAGTTAATGTGCAGATTGTCGTCTACCGTTAGGGTAACCTTAATCGGCTCTTAGCGAACAAAAT‑TGCTGGT‑CAGGTTTGta                                                                                                                                      >  1:3454219/1‑147 (MQ=255)
               tCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTGTGACTAACGCACACGGCGTTGATTTGCCGATTTGCGTCTCCGGGTTGGGGAACCTTATTCGGCTCTTCGCGACCCAATT‑GGCTGGG‑GCGGTTTGtga                                                                                                                                     >  1:1020447/1‑146 (MQ=255)
                 tttCTCACACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACGACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGa                                                                                                                                   <  2:2709631/148‑1 (MQ=255)
                 tttATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGa                                                                                                                                   <  1:928946/148‑1 (MQ=255)
                 tttATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGa                                                                                                                                   <  2:2711915/148‑1 (MQ=255)
                    aTCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACa                                                                                                                                <  1:86924/148‑1 (MQ=255)
                      cAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAat                                                                                                                              <  1:2623795/148‑1 (MQ=255)
                        aaCACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTATGACTACCTAACAGGGAGTTAATGTCCAGATTTGCGTCTCCGGTTAGGGTAACCGTATTAGGCTCTTAGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAatat                                                                                                                            >  1:616259/1‑148 (MQ=255)
                         acacGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATg                                                                                                                           <  2:1460117/148‑1 (MQ=255)
                          cacGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATgg                                                                                                                          <  1:1144956/148‑1 (MQ=255)
                          cacGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATgg                                                                                                                          <  1:770106/148‑1 (MQ=255)
                          cacGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATgg                                                                                                                          <  2:2236766/148‑1 (MQ=255)
                          cacGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTATGACAACCCAACAGGGAGTTAATTTCCAGAATTGCGTCTCCGGTTAGGGTAACCATATTAGGCTCATCGCGACCAAATT‑TGCTGGG‑GAGGTTTGTGATAACAaaattg                                                                                                                          >  1:1786873/1‑146 (MQ=255)
                             gggCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATGGGCg                                                                                                                       <  2:3589832/148‑1 (MQ=255)
                              ggCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATGGGCGt                                                                                                                      <  2:1766680/148‑1 (MQ=255)
                              ggCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATGGGCGt                                                                                                                      <  2:3230406/148‑1 (MQ=255)
                               gCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATGGGCGTc                                                                                                                     <  1:71423/148‑1 (MQ=255)
                                   cgcTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATGGGCGTCAGcc                                                                                                                 <  2:2447668/148‑1 (MQ=255)
                                       ttCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATGGGCGTCAGCCACGc                                                                                                             >  2:1913243/1‑148 (MQ=255)
                                       ttCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCCAACAGGGAGTTCATGTACAGATTGGCGTCTCCGGTTAGGGTAACCGTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CCGGTTTGTCAGAACAAAATGGGCGTCAGCCACGc                                                                                                             >  1:3418063/1‑148 (MQ=255)
                                        tCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATGGGCGTCAGCCACGcc                                                                                                            >  2:1825495/1‑148 (MQ=255)
                                         cAGCTTCCGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCg                                                                                                           <  2:154815/148‑1 (MQ=255)
                                          aGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATGGGCGTCACCCACGCCgg                                                                                                          <  2:2938712/148‑1 (MQ=255)
                                           gCTTCAGTTTCATGGTCGCTTTATGACTAACCAAAAGGGAGTTGATGTCCAGATTGGGGTCTCCGGTTAGGGTAAACTTATTAGGCTCTTCGCGACCAAATT‑TGCTGGT‑CAGGTTTTTAAGAAAAATATTTGCGTCAGCCACGCCggg                                                                                                         >  1:3635599/1‑148 (MQ=255)
                                            cTTCAGTTTCATGGTCGCTTTCTGACTACCCAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAAATT‑GGGTGGT‑GAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGt                                                                                                        >  2:3735967/1‑148 (MQ=255)
                                               cAGTTTCTTGGTCGCTTTCTGCCTACCGAACCGGGTGTTCATGTCCAGTTTGGGGTCTCGGGTTTGGGTAACCTTTTGCGGCTCTTCGCGACCATTTT‑GGCTGGT‑CAGGTTTGTCAGAAAAATTTGGGCGTCAGCCACGCCGGGTaaa                                                                                                     <  2:1133451/148‑1 (MQ=255)
                                               cAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTaaa                                                                                                     <  2:630936/148‑1 (MQ=255)
                                                  tttCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACAAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCg                                                                                                  <  2:1506855/148‑1 (MQ=255)
                                                      ttGGTCGCTTTCTCACTACCCAACAGGTAGTACTTGTCCAGTTTGGCTTCTCCGGTTTGGGTAACGTTTTTCGGCTCTTCGCGACCATTTG‑GGCTGTT‑CTGTTTTCTAACAAAAATATGGGCGACAGCCACGCCGGGTAAACCGatat                                                                                              <  1:536392/147‑1 (MQ=255)
                                                       tGGTCGCTTTCTGACTACCGAACCGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATc                                                                                             <  2:1395961/148‑1 (MQ=255)
                                                        ggTCGCTTTCTGACTACCGAACTGGGTGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCTGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCt                                                                                            <  2:910245/148‑1 (MQ=255)
                                                         gTCGCTTTCTGACTACCGAACAGGGTGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CTGGTTTGTCATAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCtt                                                                                           <  2:1357653/148‑1 (MQ=255)
                                                           cGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCtttt                                                                                         >  2:2659228/1‑148 (MQ=255)
                                                           cGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCtttt                                                                                         >  2:406716/1‑148 (MQ=255)
                                                            gCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTg                                                                                        <  2:412189/148‑1 (MQ=255)
                                                               ttCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTgaga                                                                                     >  2:15988/1‑148 (MQ=255)
                                                                  tGACTACCGAACAGGGATTTCATGTCCATATTGGCGTCTCCGGTTAGGTTAACCTTATGCGGCTCTTCGCGACAAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAat                                                                                  <  1:2218493/148‑1 (MQ=255)
                                                                   gACTACCGAACGGGGTGTTCAGGTCACTTTGGGGGTCTCGGGTTTGGGTAACCTTTTTCGGCTCTGCGCGACCATTTG‑GGGTGTT‑CTGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAatt                                                                                 <  1:2137570/148‑1 (MQ=255)
                                                                   gACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCGGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAatt                                                                                 >  1:1345349/1‑148 (MQ=255)
                                                                   gACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAatt                                                                                 >  1:1562017/1‑148 (MQ=255)
                                                                    acaaCCGAACGGGGTGTTCATGACCAGTTGGGCGTCTCCGTTTTGGGTAACCTTATTGGGCTCTTCGCGACAAATTT‑GGCTGGT‑CTTGTTTTTCACAAAAATTGGGGCGTCACCCACGCCGGGTAAACCGATATCTTTTGAGAAatta                                                                                <  1:3282546/145‑1 (MQ=255)
                                                                    aCTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAatta                                                                                >  1:1995971/1‑148 (MQ=255)
                                                                     cTACCGAACAGGTTTTTCTGGTCCATTTTGGCGTCTCGGTTTTGGGTAACTTTATTCGGCTCTTCGCGACAATTTT‑GGGTGGT‑CTGTTTTGTCATAACAATATGGGCGACAGCCACGCCGGGTAAACCGATATCTTTTGAGAAattat                                                                               <  1:1996954/148‑1 (MQ=255)
                                                                      tACCGAACAGGGTGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAattatt                                                                              <  2:1597887/148‑1 (MQ=255)
                                                                      tACCGAACAGGGATTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTATTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCATAAAAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAattatt                                                                              <  2:495660/148‑1 (MQ=255)
                                                                       aCCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGTTGGT‑CAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAAttatta                                                                             >  1:655847/1‑148 (MQ=255)
                                                                        ccGACCAGGGATTTAATGTCCCGATTGGCGTCGCGGGTTTGGGTAACATTATTGGGATCTGCGCGACAATTTT‑GGGTGGT‑ATGGTTTTTAAGAAAAATTTGGGCGTCCGCCACGCCGGGTAAACCGATATCTTTTGAGAAATtattat                                                                            <  1:1969977/148‑1 (MQ=255)
                                                                          gAACAGTGAGTTCATTTCCTGATTGGCTTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTTTT‑CAGGTTTTTCTTAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATATTTTGAGATATTATTATGt                                                                          <  2:2345448/148‑1 (MQ=255)
                                                                                ggAGTTCTTGTCCAGTTTGGCGTCTCCGTTTAGGTTAACCTTTTTCGGCTCTTCGCGACAATTTT‑GGGTGGT‑CTGGTTTGTCACAACAATATGGGCGACACCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCa                                                                    <  1:3666533/148‑1 (MQ=255)
                                                                                ggAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCa                                                                    <  2:2957210/148‑1 (MQ=255)
                                                                                  aGTTCATGTCCAGATTGGCGTCTCCGTTTAGGGTAACATTATTCGGCTCTTCGCTACCAATTT‑GGCTGGT‑CAGTTTTGACATAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAg                                                                  <  2:757001/148‑1 (MQ=255)
                                                                                     tcttGTCCAGATTGGCGGCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGa                                                               <  2:1432992/145‑1 (MQ=255)
                                                                                     tCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGAACTTTTT‑GGCTGGT‑CAGGTTTGTCAGAACATTATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGa                                                               <  2:3388140/148‑1 (MQ=255)
                                                                                       ttGTCCTGTTTGGCGTCTCGGGTTAGGTTAACCTTATTCGGCTCTTCGCGAAAATTTT‑GGCTTGT‑CTGTTTTGTCATAAAAATAGGGGCGTCCGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACt                                                             <  1:2849148/147‑1 (MQ=255)
                                                                                          tCCTGATTGGCGTGTCCGGTTCGGGTAACCTTATTCGGCTCTTCGCGCCCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACATTATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGt                                                          <  2:3402369/148‑1 (MQ=255)
                                                                                            ctgATGGGCGTCTCGGGTTTGGTTAACCTTTTTCGGCTGTTCGCGACAATTTT‑GGCTGTT‑CTGTTTTTTAATAACTTTTTGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTTTTATGTTTCGCAAGCGACTGGTTa                                                        <  2:1774684/146‑1 (MQ=255)
                                                                                            cAGATTGGCGTCTCCGGTTTGGGTAACCTTATTCGGCTCTTCGCCACCATTTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTa                                                        <  2:2178627/148‑1 (MQ=255)
                                                                                              gATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAAt                                                      >  1:1390820/1‑148 (MQ=255)
                                                                                               aTTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAAtt                                                     >  1:1827623/1‑148 (MQ=255)
                                                                                               aTTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTGTGAGAAATTAGTATGTTTCGCAAGCGACTGGTTAAtt                                                     >  2:2206667/1‑148 (MQ=255)
                                                                                                  ggggTCTCGGGTTTGTGAAACCTTTTTCGCCTCTACGCCACAATTTT‑GGCTGGT‑CCGTTTTGTAAAAAAAATAGGGGCTTCACCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCt                                                  <  2:656181/145‑1 (MQ=255)
                                                                                                   gCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCtt                                                 >  1:354351/1‑148 (MQ=255)
                                                                                                   gCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCtt                                                 >  1:2658234/1‑148 (MQ=255)
                                                                                                      tctcCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCATTTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGtt                                              <  2:2451878/148‑1 (MQ=255)
                                                                                                      tctcCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGtt                                              >  1:2964825/1‑148 (MQ=255)
                                                                                                         cggttttgggTAACTTTTTCGGGCTCTCCGCCACAATTTT‑GGGTGGT‑CTGTTTTGTAACAAAAATTGGGGCGCCACCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCgg                                           <  1:1494053/141‑1 (MQ=255)
                                                                                                         ccGGTTTGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCgg                                           <  2:1167840/148‑1 (MQ=255)
                                                                                                         ccGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCgg                                           >  2:2558389/1‑148 (MQ=255)
                                                                                                         ccGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTGAATTTCTTGTTCgg                                           >  2:2558709/1‑148 (MQ=255)
                                                                                                           ggTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGTT‑CAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTg                                         <  2:3142631/148‑1 (MQ=255)
                                                                                                           ggTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTg                                         <  2:2824071/148‑1 (MQ=255)
                                                                                                           ggTTAGGGTAACCTTATTCGGCTCTTCGCGACAAATTT‑GGCTGGT‑CAGGTTTGTCATAACAATTTGGGCGACAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTg                                         <  2:2591319/148‑1 (MQ=255)
                                                                                                             ttAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGAt                                       >  2:2449604/1‑148 (MQ=255)
                                                                                                             ttAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGAt                                       >  1:3523575/1‑148 (MQ=255)
                                                                                                                gggAAACTTTATGCGGCTCTCCGCCA‑CAATTT‑GGGGGGTCCTTGTTTGTCACAAAATTTGGGGCGCCCCCCACGCCGGGTAAACCTATATCTTTTGATAATTTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGgt                                    <  1:1449005/148‑1 (MQ=255)
                                                                                                                     acattATGCGGCTCTCCGCCACCATTTG‑GGCGGGT‑CTGGTTGGACACAAAATTTGGGGCGACACCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTAtt                               <  1:1128584/145‑1 (MQ=255)
                                                                                                                          ttgcGGCTCTTCGCGACCAATTG‑GGCTGGT‑CTGTTTTGTAATAACAATCGGGGCGACACCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTg                          <  1:2402925/145‑1 (MQ=255)
                                                                                                                          ttgcGGCTCTGCGCGACAATTTG‑GGGTGGT‑CTGGTTTGAAACAAAAATCGGGGCGACACCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTg                          <  1:1825495/145‑1 (MQ=255)
                                                                                                                          aTTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATGGGCGTCAGCCATGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTg                          <  1:1827065/148‑1 (MQ=255)
                                                                                                                            tCGGCACTTCGCGACCATTTT‑GGCTGTA‑CAGGTTTGAAATAACAATATGGGCCTCACCCACGCCGGGTAAACCGATATATTTTGATAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAg                        <  2:1956432/148‑1 (MQ=255)
                                                                                                                            gcgcctctTCCCCAACATTGT‑GGCTGGT‑CTGGTTTTTCACAAAAATGGGGCGGCCACCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAg                        <  1:2994602/144‑1 (MQ=255)
                                                                                                                                  ctTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGAtt                  >  1:848364/1‑148 (MQ=255)
                                                                                                                                  ctTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGAtt                  >  1:2131886/1‑148 (MQ=255)
                                                                                                                                     ccgccacAATTTGGGCTGGT‑CTGTTTTGACAAAACATTTGGGGCGCCCCCCACGCCGGTTAAACCGATATATTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTAc                <  1:2320594/142‑1 (MQ=255)
                                                                                                                                      gcgACCATTTT‑GGCTGTT‑CAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAg              <  1:3812414/148‑1 (MQ=255)
                                                                                                                                           cAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAAc         >  1:2442510/1‑148 (MQ=255)
                                                                                                                                             aTTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGa       >  2:32503/1‑148 (MQ=255)
                                                                                                                                                tgggCTGGT‑CTGGTTTGAAACAAAAATAGGGGCGACAGCCACGCCGGGTAAACCTATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGc     <  1:621054/146‑1 (MQ=255)
                                                                                                                                                   gCTGTT‑CTGGTTTGTCATAACAATATGGGGGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAgg  <  2:2739600/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGT‑CAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGG  >  CP009273/1118395‑1118688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑