Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,122,429 G→T 17.2% S56S (TCG→TCT)  yceM → putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,122,4290GT17.2% 633.6 / 75.1 214S56S (TCG→TCT) yceMputative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (78/99);  new base T (15/22);  total (93/121)
Fisher's exact test for biased strand distribution p-value = 7.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 215 total aligned reads displayed.

CGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACG  >  CP009273/1122282‑1122575
                                                                                                                                                   |                                                                                                                                                  
cGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCg                                                                                                                                                    <  2:787027/148‑1 (MQ=255)
 gTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGt                                                                                                                                                   <  2:2374217/148‑1 (MQ=255)
   aGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCTTTa                                                                                                                                                 <  2:441806/148‑1 (MQ=255)
   aGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTa                                                                                                                                                 <  1:1549293/148‑1 (MQ=255)
    gTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCTTTAt                                                                                                                                                <  2:286431/148‑1 (MQ=255)
     tGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATc                                                                                                                                               <  2:1089434/148‑1 (MQ=255)
     tGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATc                                                                                                                                               <  1:2814880/148‑1 (MQ=255)
          ttAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCTTTATCCAGcc                                                                                                                                          >  1:957839/1‑148 (MQ=255)
           tAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCt                                                                                                                                         <  2:3131247/148‑1 (MQ=255)
             ggTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTg                                                                                                                                       >  2:1512363/1‑148 (MQ=255)
                  cATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCTTTATCCAGCCTTgccgcc                                                                                                                                  >  2:3813048/1‑148 (MQ=255)
                  cATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTgccgcc                                                                                                                                  >  2:3036076/1‑148 (MQ=255)
                     tGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTCCGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGt                                                                                                                               >  2:1824247/1‑148 (MQ=255)
                     tGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCTTTCCTTATGACGATTCGTTATCCAGCCTTGCCGCCAGt                                                                                                                               <  2:622701/148‑1 (MQ=255)
                         cAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCg                                                                                                                           <  1:3587632/148‑1 (MQ=255)
                           aaaaGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCTTTATCCAGCCTTGCCGCCAGTTGCGAt                                                                                                                         >  2:3802037/1‑148 (MQ=255)
                           aaaaGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGAt                                                                                                                         >  2:204360/1‑148 (MQ=255)
                            aaaGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCTTTATCCAGCCTTGCCGCCAGTTGCGATg                                                                                                                        >  2:2873267/1‑148 (MQ=255)
                               gCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCgg                                                                                                                     >  1:2088623/1‑148 (MQ=255)
                                cGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGt                                                                                                                    >  1:1804661/1‑148 (MQ=255)
                                  tGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGttt                                                                                                                  >  1:1213381/1‑148 (MQ=255)
                                  tGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGttt                                                                                                                  <  2:326078/148‑1 (MQ=255)
                                     ttACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCTTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTtg                                                                                                               <  2:2590382/148‑1 (MQ=255)
                                     ttACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCTTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTtg                                                                                                               <  2:2587615/148‑1 (MQ=255)
                                          ggTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCAt                                                                                                          <  2:711367/148‑1 (MQ=255)
                                          ggTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCAt                                                                                                          >  2:2105006/1‑148 (MQ=255)
                                                    gcAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCg                                                                                                >  2:654330/1‑148 (MQ=255)
                                                    gcAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCg                                                                                                >  2:1555896/1‑148 (MQ=255)
                                                     cAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCTTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCgc                                                                                               >  2:704549/1‑148 (MQ=255)
                                                        cGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCgccag                                                                                            >  1:679741/1‑148 (MQ=255)
                                                        cGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCgccag                                                                                            >  1:2191588/1‑148 (MQ=255)
                                                              aCTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCTTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACtt                                                                                      >  1:3814289/1‑148 (MQ=255)
                                                                tGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCTTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTg                                                                                    <  1:1326008/148‑1 (MQ=255)
                                                                tGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTg                                                                                    >  1:1706649/1‑148 (MQ=255)
                                                                tGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTg                                                                                    <  1:3663147/148‑1 (MQ=255)
                                                                 ggACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGa                                                                                   <  1:3518217/148‑1 (MQ=255)
                                                                 ggACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGa                                                                                   >  1:85472/1‑148 (MQ=255)
                                                                  gACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCTTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGAc                                                                                  >  2:2526447/1‑148 (MQ=255)
                                                                     gTTACAAGGAGTCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCTTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTg                                                                               <  1:220674/148‑1 (MQ=255)
                                                                     gTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTg                                                                               <  1:1515019/148‑1 (MQ=255)
                                                                         cAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCa                                                                           <  1:1901863/148‑1 (MQ=255)
                                                                              aGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACg                                                                      <  2:1164150/148‑1 (MQ=255)
                                                                                   ggTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCTTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACt                                                                 <  2:1931350/148‑1 (MQ=255)
                                                                                   ggTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACt                                                                 <  1:3291387/148‑1 (MQ=255)
                                                                                   ggTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACt                                                                 <  1:12808/148‑1 (MQ=255)
                                                                                   ggTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACt                                                                 <  1:3357636/148‑1 (MQ=255)
                                                                                    gTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTc                                                                >  1:3317109/1‑148 (MQ=255)
                                                                                     tCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCTTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCa                                                               <  1:3290130/148‑1 (MQ=255)
                                                                                     tCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCa                                                               >  1:548639/1‑148 (MQ=255)
                                                                                     tCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCa                                                               >  1:926639/1‑148 (MQ=255)
                                                                                      cGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCaa                                                              <  2:224878/148‑1 (MQ=255)
                                                                                      cGCCTACGCGCGCGAAAGCCCTACCACTTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCaa                                                              <  2:2154912/148‑1 (MQ=255)
                                                                                       gCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAAt                                                             <  1:168679/148‑1 (MQ=255)
                                                                                        ccTACGCGCGCTCAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCTTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATg                                                            <  2:3749110/148‑1 (MQ=255)
                                                                                          tACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCg                                                          <  2:2088412/148‑1 (MQ=255)
                                                                                           aCGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCgg                                                         >  2:1176286/1‑148 (MQ=255)
                                                                                           aCGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCgg                                                         >  2:3728410/1‑148 (MQ=255)
                                                                                              cgcgcgAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCggggg                                                      <  2:1253074/148‑1 (MQ=255)
                                                                                              cgcgcgAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCggggg                                                      <  1:2452001/148‑1 (MQ=255)
                                                                                               gcgcgAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGt                                                     <  1:1457709/148‑1 (MQ=255)
                                                                                               gcgcgAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGt                                                     <  1:2384523/148‑1 (MQ=255)
                                                                                                 gcgAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTAc                                                   <  1:1512363/148‑1 (MQ=255)
                                                                                                 gcgAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTAc                                                   <  1:3154453/148‑1 (MQ=255)
                                                                                                  cgAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACa                                                  <  2:3445226/148‑1 (MQ=255)
                                                                                                    aaaGCCCTGCCAATGTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATg                                                <  2:1963839/148‑1 (MQ=255)
                                                                                                     aaGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCTTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGt                                               <  2:350892/148‑1 (MQ=255)
                                                                                                      aGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTc                                              <  1:1833072/148‑1 (MQ=255)
                                                                                                      aGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTc                                              <  1:2975257/148‑1 (MQ=255)
                                                                                                       gCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCt                                             <  1:3766631/148‑1 (MQ=255)
                                                                                                            gCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTc                                        >  1:73615/1‑148 (MQ=255)
                                                                                                             ccAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCg                                       >  2:1862726/1‑148 (MQ=255)
                                                                                                                 tttGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATaa                                   <  1:1887566/148‑1 (MQ=255)
                                                                                                                     tgAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCg                               <  1:3041544/148‑1 (MQ=255)
                                                                                                                     tgAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCg                               >  1:2482004/1‑148 (MQ=255)
                                                                                                                      gAAAGCTGGCGCATTCCTTATGCCGATTCTTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGc                              <  2:30445/148‑1 (MQ=255)
                                                                                                                      gAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGc                              >  2:1621593/1‑148 (MQ=255)
                                                                                                                       aaaGCTGGCGCATTCCTTATGCCGATTCTTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCt                             >  2:537386/1‑148 (MQ=255)
                                                                                                                       aaaGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCt                             >  1:3362283/1‑148 (MQ=255)
                                                                                                                         aGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTgg                           >  2:2431024/1‑148 (MQ=255)
                                                                                                                         aGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTgg                           >  2:274634/1‑148 (MQ=255)
                                                                                                                          gCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGc                          >  1:2568438/1‑148 (MQ=255)
                                                                                                                          gCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGc                          <  2:1143451/148‑1 (MQ=255)
                                                                                                                           cTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCa                         >  1:2675505/1‑148 (MQ=255)
                                                                                                                             ggCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGa                       >  1:3809595/1‑148 (MQ=255)
                                                                                                                              gcgcATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGaa                      >  2:1288945/1‑148 (MQ=255)
                                                                                                                               cgcATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGaaa                     <  2:3479036/148‑1 (MQ=255)
                                                                                                                                 cATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAAt                   <  2:949951/148‑1 (MQ=255)
                                                                                                                                  aTTCCTTATGCCGATTCTTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATc                  <  1:2022823/148‑1 (MQ=255)
                                                                                                                                    tCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTg                >  2:2778223/1‑148 (MQ=255)
                                                                                                                                     ccTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTgc               >  1:1009356/1‑148 (MQ=255)
                                                                                                                                      cTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTgcg              <  2:3316430/148‑1 (MQ=255)
                                                                                                                                           gCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATg         <  2:365666/148‑1 (MQ=255)
                                                                                                                                           gCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATg         >  2:2548592/1‑148 (MQ=255)
                                                                                                                                           gCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATg         <  2:1480862/148‑1 (MQ=255)
                                                                                                                                              gATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTg      >  1:3620785/1‑148 (MQ=255)
                                                                                                                                               aTTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGa     <  1:204360/148‑1 (MQ=255)
                                                                                                                                                ttCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGaa    <  2:2995258/148‑1 (MQ=255)
                                                                                                                                                ttCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGaa    >  2:2415164/1‑148 (MQ=255)
                                                                                                                                                 tCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAAc   >  1:817374/1‑148 (MQ=255)
                                                                                                                                                  cGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACg  <  1:2333862/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCGAAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTTGCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTCAGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTGCGCGATGCTGAACG  >  CP009273/1122282‑1122575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑