Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,122,994 C→A 100% R245S (CGC→AGC)  yceM → putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,122,9940CA96.2% 553.2 / 12.2 183R245S (CGC→AGC) yceMputative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (4/3);  new base A (95/81);  total (99/84)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 184 total aligned reads displayed.

TTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCTG  >  CP009273/1122850‑1123137
                                                                                                                                                |                                                                                                                                               
tttGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGagcg                                                                                                                                              <  1:240905/148‑1 (MQ=255)
  tGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCggg                                                                                                                                            >  2:2404067/1‑148 (MQ=255)
    ccGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCa                                                                                                                                          >  1:3037897/1‑148 (MQ=255)
    ccGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCa                                                                                                                                          <  1:1680090/148‑1 (MQ=255)
      gAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAgg                                                                                                                                        <  1:122095/148‑1 (MQ=255)
       aGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAggg                                                                                                                                       <  2:368504/148‑1 (MQ=255)
       aGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAggg                                                                                                                                       >  2:2340732/1‑148 (MQ=255)
         cACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCg                                                                                                                                     <  2:2185595/148‑1 (MQ=255)
            cATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAg                                                                                                                                  <  2:2415917/148‑1 (MQ=255)
               ttttCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGc                                                                                                                               >  2:1151752/1‑148 (MQ=255)
               ttttCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGc                                                                                                                               >  2:1697986/1‑148 (MQ=255)
                tttCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCa                                                                                                                              <  1:921972/148‑1 (MQ=255)
                   cGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATaa                                                                                                                           <  2:3432205/148‑1 (MQ=255)
                    ggCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATaaa                                                                                                                          <  2:2761636/148‑1 (MQ=255)
                     gCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAAc                                                                                                                         <  1:2125391/148‑1 (MQ=255)
                     gCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAAc                                                                                                                         <  2:3515503/148‑1 (MQ=255)
                          tCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGAtt                                                                                                                    <  1:19289/148‑1 (MQ=255)
                           ccTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTc                                                                                                                   <  2:2198308/148‑1 (MQ=255)
                           ccTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTc                                                                                                                   <  2:3666677/148‑1 (MQ=255)
                            cTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTcc                                                                                                                  <  1:916165/148‑1 (MQ=255)
                            cTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTcc                                                                                                                  >  1:3338374/1‑148 (MQ=255)
                             tttGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCt                                                                                                                 <  2:2542866/148‑1 (MQ=255)
                              ttGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTg                                                                                                                >  2:760456/1‑148 (MQ=255)
                               tGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTgg                                                                                                               >  1:2160930/1‑148 (MQ=255)
                               tGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTgg                                                                                                               >  1:2160924/1‑148 (MQ=255)
                                  aGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTg                                                                                                            <  2:3286154/148‑1 (MQ=255)
                                    aTCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGc                                                                                                          >  2:3221633/1‑148 (MQ=255)
                                    aTCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGc                                                                                                          >  2:1166796/1‑148 (MQ=255)
                                     tCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCa                                                                                                         >  1:388201/1‑148 (MQ=255)
                                      caccacCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCag                                                                                                        <  2:3284773/148‑1 (MQ=255)
                                       accaccTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCaga                                                                                                       >  2:1377384/1‑148 (MQ=255)
                                         caccTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGt                                                                                                     <  2:2106210/148‑1 (MQ=255)
                                         caccTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGt                                                                                                     <  2:1946891/148‑1 (MQ=255)
                                               tATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCtt                                                                                               >  2:375396/1‑148 (MQ=255)
                                               tATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCtt                                                                                               >  2:1315866/1‑148 (MQ=255)
                                                aTGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTg                                                                                              <  1:3117236/148‑1 (MQ=255)
                                                aTGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTg                                                                                              >  2:3688116/1‑148 (MQ=255)
                                                 tGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGa                                                                                             >  2:1796578/1‑148 (MQ=255)
                                                  gCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAg                                                                                            >  2:1614919/1‑148 (MQ=255)
                                                  gCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAg                                                                                            >  2:1614824/1‑148 (MQ=255)
                                                  gCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAg                                                                                            >  2:801822/1‑148 (MQ=255)
                                                  gCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAg                                                                                            >  2:492804/1‑148 (MQ=255)
                                                    aTCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCa                                                                                          <  2:3209105/148‑1 (MQ=255)
                                                    aTCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCa                                                                                          <  1:1613818/148‑1 (MQ=255)
                                                      cGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAAc                                                                                        >  1:1602828/1‑148 (MQ=255)
                                                           tGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTggg                                                                                   <  1:2697680/148‑1 (MQ=255)
                                                            gCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTTCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGt                                                                                  <  2:1072575/148‑1 (MQ=255)
                                                            gCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGt                                                                                  <  1:3218023/148‑1 (MQ=255)
                                                               ggAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTg                                                                               <  1:1654909/148‑1 (MQ=255)
                                                                 aaGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTc                                                                             >  2:3200059/1‑148 (MQ=255)
                                                                 aaGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTc                                                                             >  2:3201087/1‑148 (MQ=255)
                                                                   gTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCgg                                                                           >  2:3275843/1‑148 (MQ=255)
                                                                    tCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGc                                                                          >  1:539794/1‑148 (MQ=255)
                                                                      aGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACg                                                                                                  <  1:1426424/122‑1 (MQ=255)
                                                                      aGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACg                                                                                                  >  2:1426424/1‑122 (MQ=255)
                                                                        cGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCtgtg                                                                      <  2:2739486/148‑1 (MQ=255)
                                                                                cGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCAc                                                              <  2:2824648/148‑1 (MQ=255)
                                                                                cGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCAc                                                              <  2:2824556/148‑1 (MQ=255)
                                                                                   gCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTc                                                           <  2:1561873/148‑1 (MQ=255)
                                                                                   gCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTc                                                           <  1:1982095/148‑1 (MQ=255)
                                                                                     aGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCAt                                                         <  1:3715338/148‑1 (MQ=255)
                                                                                     aGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCAt                                                         <  2:698966/148‑1 (MQ=255)
                                                                                     aGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCAt                                                         <  2:698895/148‑1 (MQ=255)
                                                                                       gCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTg                                                       >  2:2112830/1‑148 (MQ=255)
                                                                                       gCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTg                                                       >  2:3415921/1‑148 (MQ=255)
                                                                                          gTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAAt                                                    <  1:1507389/148‑1 (MQ=255)
                                                                                           tgactgacGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAAtg                                                   <  1:492804/148‑1 (MQ=255)
                                                                                            gactgacGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAAtgt                                                  >  2:2965207/1‑148 (MQ=255)
                                                                                             actgacGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAAtgtg                                                 <  1:3275843/148‑1 (MQ=255)
                                                                                             actgacGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAAtgtg                                                 <  1:2340732/148‑1 (MQ=255)
                                                                                                   ggTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCaaa                                           >  1:747917/1‑148 (MQ=255)
                                                                                                   ggTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCaaa                                           >  1:2664181/1‑148 (MQ=255)
                                                                                                     tGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAAc                                         >  2:2864079/1‑148 (MQ=255)
                                                                                                     tGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAAc                                         >  1:2080598/1‑148 (MQ=255)
                                                                                                     tGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAAc                                         >  2:3628273/1‑148 (MQ=255)
                                                                                                       cgcTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCa                                       <  2:2691556/148‑1 (MQ=255)
                                                                                                       cgcTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTCCATTGAATGTGTGCAAAACCa                                       >  2:1108066/1‑148 (MQ=255)
                                                                                                           cATCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACa                                   <  1:3684570/148‑1 (MQ=255)
                                                                                                            aTCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAg                                  <  1:2002262/148‑1 (MQ=255)
                                                                                                             tCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGt                                 <  2:3794502/148‑1 (MQ=255)
                                                                                                             tCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGt                                 <  2:670231/148‑1 (MQ=255)
                                                                                                             tCGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGt                                 >  1:3812273/1‑148 (MQ=255)
                                                                                                              cGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGtt                                >  1:3768133/1‑148 (MQ=255)
                                                                                                              cGACATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGtt                                >  1:655994/1‑148 (MQ=255)
                                                                                                                aCATTACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTcc                              >  1:1127148/1‑148 (MQ=255)
                                                                                                                   ttACGGATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCa                           >  2:311105/1‑148 (MQ=255)
                                                                                                                        gATATGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAAccg                      >  1:203637/1‑148 (MQ=255)
                                                                                                                         atatGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAAccgc                     >  2:936518/1‑148 (MQ=255)
                                                                                                                         atatGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAAccgc                     >  1:2509135/1‑148 (MQ=255)
                                                                                                                         atatGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAAccgc                     >  1:1027547/1‑148 (MQ=255)
                                                                                                                           atGCGCGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAAccgccg                   >  2:816220/1‑148 (MQ=255)
                                                                                                                              cgcgAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCg                >  1:1900030/1‑148 (MQ=255)
                                                                                                                                cgAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGaa              <  2:802949/148‑1 (MQ=255)
                                                                                                                                   aTGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAg           >  1:856250/1‑148 (MQ=255)
                                                                                                                                   aTGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAg           >  1:1017767/1‑148 (MQ=255)
                                                                                                                                    tGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAgg          >  2:3470332/1‑148 (MQ=255)
                                                                                                                                    tGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAgg          <  2:253943/148‑1 (MQ=255)
                                                                                                                                     ggCGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGc         <  1:1748149/148‑1 (MQ=255)
                                                                                                                                      aggtttagagCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGcc        >  1:3506534/8‑148 (MQ=255)
                                                                                                                                          gaggagAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGc    >  2:3125808/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                               
TTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCTG  >  CP009273/1122850‑1123137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑