Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,146,692 A→T 100% T189T (ACA→ACT)  fabG → 3‑oxoacyl‑[acyl‑carrier‑protein] reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,146,6920AT97.9% 773.3 / 4.3 235T189T (ACA→ACT) fabG3‑oxoacyl‑[acyl‑carrier‑protein] reductase
Reads supporting (aligned to +/- strand):  ref base A (2/3);  new base T (118/112);  total (120/115)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 235 total aligned reads displayed.

GTACCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACACGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGT  >  CP009273/1146547‑1146835
                                                                                                                                                 |                                                                                                                                               
 tACCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGt                                                                                                                                              >  2:1828180/1‑148 (MQ=255)
     aTGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTgcgc                                                                                                                                          >  1:1361108/1‑148 (MQ=255)
      tGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCt                                                                                                                                         >  2:2326180/1‑148 (MQ=255)
       gggAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTg                                                                                                                                        <  2:3371116/148‑1 (MQ=255)
       gggAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGCCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTg                                                                                                                                        <  1:761869/148‑1 (MQ=255)
         gAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAg                                                                                                                                      >  1:2306003/1‑148 (MQ=255)
           aaTGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCg                                                                                                                                    <  2:3231163/148‑1 (MQ=255)
            aTGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGa                                                                                                                                   >  1:380840/1‑148 (MQ=255)
            aTGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGa                                                                                                                                   <  2:1895928/148‑1 (MQ=255)
            aTGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGa                                                                                                                                   <  2:1937234/148‑1 (MQ=255)
                cGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGAc                                                                                                                               <  1:3627728/148‑1 (MQ=255)
                 ggTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGAcc                                                                                                                              <  2:2925406/148‑1 (MQ=255)
                 ggTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGAcc                                                                                                                              <  1:2107383/148‑1 (MQ=255)
                  gTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCa                                                                                                                             >  1:1909350/1‑148 (MQ=255)
                       gCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTg                                                                                                                        >  1:3200364/1‑148 (MQ=255)
                            cTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGt                                                                                                                   >  1:3312592/1‑148 (MQ=255)
                                gCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATcc                                                                                                               <  1:3076256/148‑1 (MQ=255)
                                   gcggcgAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTgg                                                                                                            <  2:4126/148‑1 (MQ=255)
                                   gcggcgAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTgg                                                                                                            <  1:2477170/148‑1 (MQ=255)
                                       cgAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCa                                                                                                        >  2:2425620/1‑148 (MQ=255)
                                          aaGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGt                                                                                                     >  1:3650988/1‑148 (MQ=255)
                                           aGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGtt                                                                                                    >  1:564239/1‑148 (MQ=255)
                                           aGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGtt                                                                                                    >  1:2905852/1‑148 (MQ=255)
                                            gCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTc                                                                                                   <  2:1609187/148‑1 (MQ=255)
                                            gCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTc                                                                                                   >  1:1990950/1‑148 (MQ=255)
                                            gCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTc                                                                                                   <  1:3515985/148‑1 (MQ=255)
                                            gCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTc                                                                                                   >  2:907787/1‑148 (MQ=255)
                                             cGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACACGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTcc                                                                                                  <  2:371300/148‑1 (MQ=255)
                                                gCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGc                                                                                               >  2:1068249/1‑148 (MQ=255)
                                                 cTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCg                                                                                              <  2:3112017/148‑1 (MQ=255)
                                                     aTCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTc                                                                                          >  2:2552587/1‑148 (MQ=255)
                                                     aTCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTc                                                                                          >  1:2995234/1‑148 (MQ=255)
                                                      tCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCg                                                                                         >  2:618804/1‑148 (MQ=255)
                                                         gCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCt                                                                                      <  1:113056/148‑1 (MQ=255)
                                                          cTTCAGTAAATCACTGGCGCACGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTc                                                                                     >  1:3372385/1‑148 (MQ=255)
                                                           ttCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTcg                                                                                    >  2:1013236/1‑148 (MQ=255)
                                                                    tCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGcac                                                                           >  1:3093844/1‑148 (MQ=255)
                                                                      aCTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAg                                                                         >  2:2988411/1‑148 (MQ=255)
                                                                       cTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAgg                                                                        <  2:1579331/148‑1 (MQ=255)
                                                                       cTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAgg                                                                        >  1:2242872/1‑148 (MQ=255)
                                                                             cgcgAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCg                                                                  <  2:2922238/148‑1 (MQ=255)
                                                                             cgcgAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCg                                                                  <  2:2921273/148‑1 (MQ=255)
                                                                             cgcgAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCg                                                                  <  1:3474030/148‑1 (MQ=255)
                                                                               cgAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTCTCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAACTCGcc                                                                >  2:3573706/1‑148 (MQ=255)
                                                                                 aaGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCaa                                                              <  1:514985/148‑1 (MQ=255)
                                                                                  aGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAAc                                                             >  2:3274562/1‑148 (MQ=255)
                                                                                    ttGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAAcgc                                                           >  1:172810/1‑148 (MQ=255)
                                                                                    ttGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAAcgc                                                           >  1:1748669/1‑148 (MQ=255)
                                                                                     tGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGAGCACAGGAAATCGCCAAcgcg                                                          >  2:2526119/1‑148 (MQ=255)
                                                                                            cgcgGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGGTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCAt                                                   <  1:1433686/148‑1 (MQ=255)
                                                                                              cgGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTc                                                 <  2:3327613/148‑1 (MQ=255)
                                                                                              cgGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTc                                                 <  1:837493/148‑1 (MQ=255)
                                                                                               ggTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTcc                                                <  1:1613778/148‑1 (MQ=255)
                                                                                                gTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCt                                               <  2:2539442/148‑1 (MQ=255)
                                                                                                gTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCt                                               <  2:1443077/148‑1 (MQ=255)
                                                                                                 tATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACACGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTg                                              >  1:2358010/1‑148 (MQ=255)
                                                                                                  aTTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTgg                                             >  1:2161589/1‑148 (MQ=255)
                                                                                                     aCTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCAt                                          >  2:652712/1‑148 (MQ=255)
                                                                                                     aCTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACACGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCAt                                          >  2:2232315/1‑148 (MQ=255)
                                                                                                     aCTGTAAACGTTGGTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCAt                                          <  1:150830/148‑1 (MQ=255)
                                                                                                       tGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATcc                                        <  1:3783514/148‑1 (MQ=255)
                                                                                                       tGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATcc                                        <  1:1043921/148‑1 (MQ=255)
                                                                                                         tAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCTCTGAGCGTTGACCAGCGTGCGGGTATCCTGGATCAGGTTCCTGCGGGTCGCATCGGCGGCGGACAGGAAATCGCCTACGCGGTTGCATTCCTGGCATCcga                                      <  2:1193084/148‑1 (MQ=255)
                                                                                                           aaCGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCcgacg                                    <  1:981217/148‑1 (MQ=255)
                                                                                                            aCGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCcgacga                                   <  2:960134/148‑1 (MQ=255)
                                                                                                            aCGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCcgacga                                   <  2:3200364/148‑1 (MQ=255)
                                                                                                            aCGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCcgacga                                   >  1:1629571/1‑148 (MQ=255)
                                                                                                            aCGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCcgacga                                   >  1:2348842/1‑148 (MQ=255)
                                                                                                             cGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGaa                                  >  1:1173791/1‑148 (MQ=255)
                                                                                                              gttgttGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAag                                 <  2:2635324/148‑1 (MQ=255)
                                                                                                                tgttgCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAagca                               >  1:432102/1‑148 (MQ=255)
                                                                                                                 ggtgCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGGGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAagcag                              <  2:502778/146‑1 (MQ=255)
                                                                                                                  ttgCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAagcagc                             >  1:3350124/1‑148 (MQ=255)
                                                                                                                   tgCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCt                            >  2:1988858/1‑148 (MQ=255)
                                                                                                                    gCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCtt                           <  1:1287031/148‑1 (MQ=255)
                                                                                                                      tCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTAc                         <  1:6226/148‑1 (MQ=255)
                                                                                                                        cGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACAt                       <  1:2552587/148‑1 (MQ=255)
                                                                                                                         gggCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATc                      >  1:1842365/1‑148 (MQ=255)
                                                                                                                         gggCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATc                      >  1:703506/1‑148 (MQ=255)
                                                                                                                         gggCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATc                      >  1:3733615/1‑148 (MQ=255)
                                                                                                                          ggCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCa                     >  2:2236346/1‑148 (MQ=255)
                                                                                                                           gCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCAc                    >  2:3488111/1‑148 (MQ=255)
                                                                                                                            cTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGTGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACg                   >  2:3075200/1‑148 (MQ=255)
                                                                                                                            cTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGGTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACg                   <  1:2819670/148‑1 (MQ=255)
                                                                                                                                  tGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGaa             >  1:1700506/1‑148 (MQ=255)
                                                                                                                                  tGAAACGGACATGAATCGTGCGATGAGCGATGACAATCGTGCGGGTATCATGGCGCAGGTTACTGCGGGTCGCATCCGCGGCGCACAGTAAATCTCCAAAGCGTCTGCATTCATGGCATCCGACGAAGCAGCTTACATCACGGGTGaa             >  2:2027323/1‑148 (MQ=255)
                                                                                                                                     aaCGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACt          <  2:380840/148‑1 (MQ=255)
                                                                                                                                      aCGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACtt         <  1:2344940/148‑1 (MQ=255)
                                                                                                                                      aCGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACtt         <  2:3160836/148‑1 (MQ=255)
                                                                                                                                      aCGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACtt         <  2:2049642/148‑1 (MQ=255)
                                                                                                                                      aCGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACtt         <  2:1842365/148‑1 (MQ=255)
                                                                                                                                       cGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACttt        >  1:383280/1‑148 (MQ=255)
                                                                                                                                       cGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACttt        <  2:736859/148‑1 (MQ=255)
                                                                                                                                        ggACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTg       <  2:1361108/148‑1 (MQ=255)
                                                                                                                                        ggACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTg       <  2:3352479/148‑1 (MQ=255)
                                                                                                                                         gACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGc      <  1:2425620/148‑1 (MQ=255)
                                                                                                                                           cATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCAt    >  2:3006219/1‑148 (MQ=255)
                                                                                                                                            aTGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCAtg   >  1:2735291/1‑148 (MQ=255)
                                                                                                                                            aTGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCAtg   <  1:635809/148‑1 (MQ=255)
                                                                                                                                             tGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCAtgt  >  1:2190726/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GTACCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACACGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGT  >  CP009273/1146547‑1146835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑