Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,156,310 G→T 100% S233* (TCA→TAA)  fhuE ← ferric‑rhodotorulic acid outer membrane transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,156,3100GT97.9% 729.4 / 5.6 234S233* (TCA→TAA) fhuEferric‑rhodotorulic acid outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base G (4/1);  new base T (121/108);  total (125/109)
Fisher's exact test for biased strand distribution p-value = 3.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 234 total aligned reads displayed.

GCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATGAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCG  >  CP009273/1156168‑1156454
                                                                                                                                              |                                                                                                                                                
gcgcGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCg                                                                                                                                             <  2:2096835/148‑1 (MQ=255)
gcgcGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCg                                                                                                                                             <  2:1844379/148‑1 (MQ=255)
   cgGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTa                                                                                                                                          >  1:2417584/1‑148 (MQ=255)
     gTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTAtt                                                                                                                                        <  1:2560826/148‑1 (MQ=255)
      tAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTc                                                                                                                                       >  1:843465/1‑148 (MQ=255)
      tAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTc                                                                                                                                       >  1:843555/1‑148 (MQ=255)
          ccGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGt                                                                                                                                   <  2:25286/148‑1 (MQ=255)
          ccGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGt                                                                                                                                   >  2:1934901/1‑148 (MQ=255)
               ccAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAgccg                                                                                                                              >  2:1876278/1‑148 (MQ=255)
                 aGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAgccgcc                                                                                                                            >  1:500604/1‑148 (MQ=255)
                  ggTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTATGTTAGccgccg                                                                                                                           >  1:3408267/1‑148 (MQ=255)
                     aGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACa                                                                                                                        <  2:3027963/148‑1 (MQ=255)
                        gCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAAtt                                                                                                                     <  2:614268/148‑1 (MQ=255)
                           aTTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTcgc                                                                                                                  >  2:3484705/1‑148 (MQ=255)
                            ttAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTcgcg                                                                                                                 >  2:3262299/1‑148 (MQ=255)
                             tAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTcgcgc                                                                                                                >  2:1393688/1‑148 (MQ=255)
                                 aTCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCgg                                                                                                            >  1:2173064/1‑148 (MQ=255)
                                   cAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGAt                                                                                                          >  2:3459812/1‑148 (MQ=255)
                                    aaTGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGAtt                                                                                                         <  2:3759675/148‑1 (MQ=255)
                                       gcgcTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTa                                                                                                      >  2:923071/1‑148 (MQ=255)
                                          cTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCg                                                                                                   >  1:1171994/1‑148 (MQ=255)
                                            gATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTc                                                                                                 >  1:3365901/1‑148 (MQ=255)
                                            gATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATGAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTc                                                                                                 >  1:2638395/1‑148 (MQ=255)
                                             atatTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCt                                                                                                <  2:2523773/148‑1 (MQ=255)
                                             atatTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCt                                                                                                <  2:3443347/148‑1 (MQ=255)
                                             atatTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCt                                                                                                >  1:3713038/1‑148 (MQ=255)
                                                ttCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCg                                                                                             >  2:2613335/1‑148 (MQ=255)
                                                ttCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCg                                                                                             <  2:2743487/148‑1 (MQ=255)
                                                ttCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCg                                                                                             >  2:1378012/1‑148 (MQ=255)
                                                ttCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTACg                                                                                             >  2:824746/1‑148 (MQ=255)
                                                 tCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCgg                                                                                            >  2:668601/1‑148 (MQ=255)
                                                  cGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGTCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGt                                                                                           <  1:2953225/148‑1 (MQ=255)
                                                   gTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTg                                                                                          <  1:779755/148‑1 (MQ=255)
                                                    tAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGa                                                                                         <  2:1487236/148‑1 (MQ=255)
                                                      aCCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGt                                                                                       <  1:3496749/148‑1 (MQ=255)
                                                      aCCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGt                                                                                       <  2:3064014/148‑1 (MQ=255)
                                                       ccGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTg                                                                                      >  1:3796050/1‑148 (MQ=255)
                                                       ccGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTg                                                                                      <  1:2910827/148‑1 (MQ=255)
                                                          gCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGc                                                                                   <  1:658028/148‑1 (MQ=255)
                                                          gCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGc                                                                                   <  1:662522/148‑1 (MQ=255)
                                                          gCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGc                                                                                   <  1:2592573/148‑1 (MQ=255)
                                                           cTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCt                                                                                  <  2:524339/148‑1 (MQ=255)
                                                           cTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCt                                                                                  <  2:3575282/148‑1 (MQ=255)
                                                           cTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCt                                                                                  <  1:53157/148‑1 (MQ=255)
                                                           cTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCt                                                                                  <  2:1307754/148‑1 (MQ=255)
                                                                aGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATACGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTa                                                                             >  1:3178745/1‑148 (MQ=255)
                                                                 gCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTaa                                                                            <  1:563457/148‑1 (MQ=255)
                                                                   gtcgtAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGTCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAAt                                                                          <  2:1068099/148‑1 (MQ=255)
                                                                   gtcgtAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAAt                                                                          <  2:14184/148‑1 (MQ=255)
                                                                   gtcgtAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAAt                                                                          <  2:2917649/148‑1 (MQ=255)
                                                                    tcgtAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATc                                                                         >  1:1393660/1‑148 (MQ=255)
                                                                        aaGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGcc                                                                     >  1:1659455/1‑148 (MQ=255)
                                                                          gATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCcac                                                                   >  1:3806232/1‑148 (MQ=255)
                                                                            tCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACAt                                                                 <  1:1110317/148‑1 (MQ=255)
                                                                            tCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACAt                                                                 <  1:1604344/148‑1 (MQ=255)
                                                                            tCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACAt                                                                 >  2:2790711/1‑148 (MQ=255)
                                                                             cGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATa                                                                <  1:3514128/148‑1 (MQ=255)
                                                                                cTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCg                                                             <  1:2486713/148‑1 (MQ=255)
                                                                                cTAAATCAGCATCGACAATGACCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCg                                                             <  1:205238/148‑1 (MQ=255)
                                                                                cTAAATCAGCAACGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCg                                                             <  2:188546/148‑1 (MQ=255)
                                                                                   aaTCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTc                                                          <  2:1150137/148‑1 (MQ=255)
                                                                                      cAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCttt                                                       >  2:1944372/1‑148 (MQ=255)
                                                                                        gCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGt                                                     >  1:2704829/1‑148 (MQ=255)
                                                                                          aTCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTc                                                   <  1:3053163/148‑1 (MQ=255)
                                                                                            cGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCa                                                 <  1:2756763/148‑1 (MQ=255)
                                                                                            cGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCa                                                 >  1:2140166/1‑148 (MQ=255)
                                                                                            cGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCa                                                 <  1:990479/148‑1 (MQ=255)
                                                                                              aCAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAAGTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTGTGTAAATCCGCCACATCCCGTTCTTTGTTCCAGc                                               >  2:3810469/1‑148 (MQ=255)
                                                                                               cAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCt                                              >  2:3208615/1‑148 (MQ=255)
                                                                                               cAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCt                                              >  1:2636220/1‑148 (MQ=255)
                                                                                                      cGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTAt                                       >  2:780865/1‑148 (MQ=255)
                                                                                                      cGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTAt                                       <  1:644993/148‑1 (MQ=255)
                                                                                                      cGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTAt                                       >  2:1611245/1‑148 (MQ=255)
                                                                                                         gaagaaGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTcc                                    >  1:245075/1‑148 (MQ=255)
                                                                                                         gaagaaGGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACAAACCGTTCTTTGTTCCAGCTACCGTATTcc                                    >  1:1674204/1‑148 (MQ=255)
                                                                                                            gaagGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCAAGCTACCGTATTccgcc                                 >  2:2411948/1‑148 (MQ=255)
                                                                                                              agGTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCga                               >  1:687499/1‑148 (MQ=255)
                                                                                                               ggTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCgag                              <  1:2694036/148‑1 (MQ=255)
                                                                                                                gTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCgaga                             >  2:718212/1‑148 (MQ=255)
                                                                                                                gTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCgaga                             >  1:36370/1‑148 (MQ=255)
                                                                                                                gTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCgaga                             >  2:468973/1‑148 (MQ=255)
                                                                                                                gTCTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGATTTGTAAATCCTCCACATACCGTTCTTTGTTACAGATACCGTAATACGCCgaga                             >  1:2297263/1‑148 (MQ=255)
                                                                                                                gTCTTTTCACTGTTGTAGCGGTCCAGCCATGAGTCGTTATTCTGGTAGCCGCCGACAAATCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATACGCCACATACCGTTCTTTGTTACAGCTACCGTAATACGCCgaga                             >  2:331928/1‑148 (MQ=255)
                                                                                                                 tcgtttCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGAc                            <  2:1255634/145‑1 (MQ=255)
                                                                                                                  cTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACa                           >  2:2263343/1‑148 (MQ=255)
                                                                                                                  cTTTTCACTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACa                           >  2:336264/1‑148 (MQ=255)
                                                                                                                        aCTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCt                     <  1:1007391/148‑1 (MQ=255)
                                                                                                                         cTGTTGTAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCtt                    <  2:2417590/148‑1 (MQ=255)
                                                                                                                            ttgtAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTaa                 >  1:3689906/1‑148 (MQ=255)
                                                                                                                             tgtAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTaaa                <  1:1496610/148‑1 (MQ=255)
                                                                                                                              gtAGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAAt               <  1:3572714/148‑1 (MQ=255)
                                                                                                                                aGCGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTc             <  2:2330421/148‑1 (MQ=255)
                                                                                                                                  cGGTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCAc           >  2:216274/1‑148 (MQ=255)
                                                                                                                                    gTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGa         <  2:399567/148‑1 (MQ=255)
                                                                                                                                    gTCCAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGa         <  2:400268/148‑1 (MQ=255)
                                                                                                                                      ccAGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACt       >  2:352926/1‑148 (MQ=255)
                                                                                                                                        aGCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTgg     >  1:1995616/1‑148 (MQ=255)
                                                                                                                                         gCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGt    >  2:1080507/1‑148 (MQ=255)
                                                                                                                                         gCCATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGt    <  1:3459812/148‑1 (MQ=255)
                                                                                                                                          ccATTAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTc   >  1:103900/1‑148 (MQ=255)
                                                                                                                                              |                                                                                                                                                
GCGCGGTAAACCGCCCCAGGTAGGGCTATTAACATCAATGCGCTGATATTCGTAACCGGCTGAAAGCGTCGTAAGATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATGAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCG  >  CP009273/1156168‑1156454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑