Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 102,736 C→A 100% I315I (ATC→ATA)  ftsZ → GTP‑binding tubulin‑like cell division protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273102,7360CA98.5% 647.0 / 1.1 204I315I (ATC→ATA) ftsZGTP‑binding tubulin‑like cell division protein
Reads supporting (aligned to +/- strand):  ref base C (3/0);  new base A (121/80);  total (124/80)
Fisher's exact test for biased strand distribution p-value = 2.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 204 total aligned reads displayed.

CTTCGACCTGCGTCTGGATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGTGACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGC  >  CP009273/102592‑102878
                                                                                                                                                |                                                                                                                                              
  tCGACCTGCGTCTGGATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCAt                                                                                                                                           >  2:743090/1‑148 (MQ=255)
  tCGACCTGCGTCTGGATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCAt                                                                                                                                           <  2:131660/148‑1 (MQ=255)
  tCGACCTGCGTCTGGATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCGTCCGACATCGCGTCTGTGGTTATCGGTTCTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCAt                                                                                                                                           <  2:1879707/148‑1 (MQ=255)
      ccTGCGTCTGGATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGAc                                                                                                                                       >  1:3447228/1‑148 (MQ=255)
         gCGTCTGGATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACaaa                                                                                                                                    <  2:534005/148‑1 (MQ=255)
         gCGTCTGGATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACaaa                                                                                                                                    <  2:2063076/148‑1 (MQ=255)
             cTGGATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTc                                                                                                                                >  2:3055548/1‑148 (MQ=255)
              tGGATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTcc                                                                                                                               <  2:2810179/148‑1 (MQ=255)
               ggATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCt                                                                                                                              >  2:723858/1‑148 (MQ=255)
                gATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTg                                                                                                                             >  2:596435/1‑148 (MQ=255)
                  tGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGaa                                                                                                                           <  1:2340704/148‑1 (MQ=255)
                   gAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGaaa                                                                                                                          >  2:2137635/1‑148 (MQ=255)
                   gAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGaaa                                                                                                                          >  1:298448/1‑148 (MQ=255)
                   gAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGaaa                                                                                                                          >  1:3346203/1‑148 (MQ=255)
                   gAGTTCGAAACGGTAGGGAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGaaa                                                                                                                          >  2:918080/1‑148 (MQ=255)
                      ttCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCa                                                                                                                       <  2:1741189/148‑1 (MQ=255)
                      ttCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCa                                                                                                                       >  2:2799369/1‑148 (MQ=255)
                          aaaCGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCActct                                                                                                                   >  2:1019553/1‑148 (MQ=255)
                           aaCGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTg                                                                                                                  >  2:1313408/1‑148 (MQ=255)
                            aCGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGATCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTgg                                                                                                                 <  1:1238362/148‑1 (MQ=255)
                             cGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGt                                                                                                                <  1:3098422/148‑1 (MQ=255)
                                taggtaACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTAc                                                                                                             <  1:1597163/148‑1 (MQ=255)
                                  ggtaACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCa                                                                                                           <  1:108836/148‑1 (MQ=255)
                                   gtaACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCaa                                                                                                          <  2:2477428/148‑1 (MQ=255)
                                    taACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAAt                                                                                                         >  2:1516264/1‑148 (MQ=255)
                                         ccATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCa                                                                                                    >  1:2656007/1‑148 (MQ=255)
                                         ccATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAAGGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCa                                                                                                    <  1:68688/148‑1 (MQ=255)
                                           aTCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAgg                                                                                                  <  2:1068465/148‑1 (MQ=255)
                                             ccGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGtt                                                                                                >  2:874464/1‑148 (MQ=255)
                                               gTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTca                                                                                              >  2:1430490/1‑148 (MQ=255)
                                                tGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTcag                                                                                             >  1:1520442/1‑148 (MQ=255)
                                                tGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTcag                                                                                             >  2:24751/1‑148 (MQ=255)
                                                  cATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTcagca                                                                                           >  1:1962030/1‑148 (MQ=255)
                                                    tttGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCagcagc                                                                                         >  1:1207550/1‑148 (MQ=255)
                                                    tttGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCagcagc                                                                                         >  1:210876/1‑148 (MQ=255)
                                                         ttCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTg                                                                                    >  2:413643/1‑148 (MQ=255)
                                                          tCCGACAACGCGACTGTTGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGa                                                                                   <  2:33404/148‑1 (MQ=255)
                                                          tCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGa                                                                                   <  2:312563/148‑1 (MQ=255)
                                                          tCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGa                                                                                   <  2:3721476/148‑1 (MQ=255)
                                                             gACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATgg                                                                                <  2:2673115/148‑1 (MQ=255)
                                                              aCAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGa                                                                               >  2:1966588/1‑148 (MQ=255)
                                                                 aCGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCg                                                                            <  1:3627447/148‑1 (MQ=255)
                                                                  cgcgACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGc                                                                           >  1:1608065/1‑148 (MQ=255)
                                                                  cgcgACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGc                                                                           >  1:2257461/1‑148 (MQ=255)
                                                                     gACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTAc                                                                        <  2:813466/148‑1 (MQ=255)
                                                                      aCTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTAcc                                                                       >  1:3515150/1‑148 (MQ=255)
                                                                      aCTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTAcc                                                                       <  1:315841/148‑1 (MQ=255)
                                                                       cTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACca                                                                      >  2:391300/1‑148 (MQ=255)
                                                                         gtgGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACcagc                                                                    >  2:2174732/1‑148 (MQ=255)
                                                                          tgGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACcagca                                                                   >  1:2936651/1‑148 (MQ=255)
                                                                           ggTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACcagcag                                                                  >  1:861051/1‑148 (MQ=255)
                                                                            gTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCagcagc                                                                 <  1:2543368/148‑1 (MQ=255)
                                                                            gTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCagcagc                                                                 <  1:2542494/148‑1 (MQ=255)
                                                                            gTTATCGGTACTACTCTTGACGCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTTCGACAGGTATAGGCCTGGACAAACTTCCTGAAATCACTCTGGTTACCAATAAGCAGGGTCAGCAGGCAGTGATGGATCGCTACcagcaga                                                                 >  2:2680417/1‑147 (MQ=255)
                                                                             ttATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAgcagca                                                                >  2:2526580/1‑148 (MQ=255)
                                                                              tATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCAt                                                               >  2:2247105/1‑148 (MQ=255)
                                                                              tATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCAt                                                               <  1:2609026/148‑1 (MQ=255)
                                                                              tATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCAt                                                               >  1:1124578/1‑148 (MQ=255)
                                                                                  ggTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGa                                                           <  1:387476/148‑1 (MQ=255)
                                                                                       ttCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCt                                                      <  1:1994709/148‑1 (MQ=255)
                                                                                        tctcTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTc                                                     >  1:354920/1‑148 (MQ=255)
                                                                                        tctcTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTc                                                     >  2:3758069/1‑148 (MQ=255)
                                                                                        tctcTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTc                                                     >  2:278694/1‑148 (MQ=255)
                                                                                          tctTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCg                                                   <  2:3447228/148‑1 (MQ=255)
                                                                                          tctTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCg                                                   >  1:3073137/1‑148 (MQ=255)
                                                                                           ctTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGc                                                  >  1:1557023/1‑148 (MQ=255)
                                                                                               aCCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGAc                                              >  2:2326409/1‑148 (MQ=255)
                                                                                               aCCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGAc                                              >  2:2463599/1‑148 (MQ=255)
                                                                                                 ccGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGAccc                                            <  2:3710505/148‑1 (MQ=255)
                                                                                                    gATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAgg                                         <  2:1809516/148‑1 (MQ=255)
                                                                                                    gATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAgg                                         <  2:3411192/148‑1 (MQ=255)
                                                                                                     atatGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGa                                        >  2:3559235/1‑148 (MQ=255)
                                                                                                      tatGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAg                                       >  1:2251175/1‑148 (MQ=255)
                                                                                                      tatGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAg                                       >  1:1287536/1‑148 (MQ=255)
                                                                                                      tatGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAg                                       >  1:596418/1‑148 (MQ=255)
                                                                                                         gaatgaCGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAg                                    >  2:1523398/1‑148 (MQ=255)
                                                                                                         gaatgaCGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAg                                    >  1:2431155/1‑148 (MQ=255)
                                                                                                          aatgaCGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGa                                   >  2:2663555/1‑148 (MQ=255)
                                                                                                          aatgaCGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGa                                   >  2:567794/1‑148 (MQ=255)
                                                                                                             gaCGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGc                                <  2:3350506/148‑1 (MQ=255)
                                                                                                              aCGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGcc                               >  2:3248479/1‑148 (MQ=255)
                                                                                                                 aGCTGCGCGTAACCGTTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGTGACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGt                            >  1:3043784/1‑148 (MQ=255)
                                                                                                                 aGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGt                            <  1:3351228/148‑1 (MQ=255)
                                                                                                                   cTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTg                          <  2:2340716/148‑1 (MQ=255)
                                                                                                                      cgcgTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTa                       >  2:1338165/1‑148 (MQ=255)
                                                                                                                      cgcgTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTa                       >  2:774352/1‑148 (MQ=255)
                                                                                                                        cgTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTaaa                     <  1:2324930/148‑1 (MQ=255)
                                                                                                                        cgTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTaaa                     <  1:723858/148‑1 (MQ=255)
                                                                                                                           aaCCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTc                  >  2:1478714/1‑148 (MQ=255)
                                                                                                                            aCCGTTGTTGCTACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCg                 <  2:3787147/148‑1 (MQ=255)
                                                                                                                            aCCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCg                 >  2:289767/1‑148 (MQ=255)
                                                                                                                            aCCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCg                 >  1:2427903/1‑148 (MQ=255)
                                                                                                                            aCCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCg                 <  1:3055548/148‑1 (MQ=255)
                                                                                                                             ccGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGt                >  2:611596/1‑148 (MQ=255)
                                                                                                                             ccGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGt                >  1:1050055/1‑148 (MQ=255)
                                                                                                                                ttgttgCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGaa             <  1:448634/148‑1 (MQ=255)
                                                                                                                                     gCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACa        <  2:772325/148‑1 (MQ=255)
                                                                                                                                        aCAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATg     <  2:3638578/148‑1 (MQ=255)
                                                                                                                                          aGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATgcg   >  1:1490307/1‑148 (MQ=255)
                                                                                                                                           ggTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATgcgc  >  2:3243677/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                              
CTTCGACCTGCGTCTGGATGAGTTCGAAACGGTAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGTGACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGC  >  CP009273/102592‑102878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑