Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,181,504 G→T 100% D69Y (GAT→TAT)  pepT → peptidase T

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,181,5040GT97.7% 736.7 / 5.9 233D69Y (GAT→TAT) pepTpeptidase T
Reads supporting (aligned to +/- strand):  ref base G (5/1);  new base T (117/110);  total (122/111)
Fisher's exact test for biased strand distribution p-value = 2.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 237 total aligned reads displayed.

AGGGGTGAGACAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCGATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTATCACCGGTTATGT  >  CP009273/1181359‑1181648
                                                                                                                                                 |                                                                                                                                                
aGGGGTGAGACAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTat                                                                                                                                                <  1:3337753/148‑1 (MQ=255)
aGGGGTGAGACAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCATGGCTat                                                                                                                                                <  1:3791003/148‑1 (MQ=255)
 ggggTGAGACAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCtata                                                                                                                                               >  1:923795/1‑148 (MQ=255)
 ggggTGAGACAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCtata                                                                                                                                               >  1:923072/1‑148 (MQ=255)
  gggTGAGACAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTatat                                                                                                                                              <  1:2694349/148‑1 (MQ=255)
  gggTGAGACAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTatat                                                                                                                                              >  1:3310818/1‑148 (MQ=255)
   ggTGAGACAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATc                                                                                                                                             <  1:2260773/148‑1 (MQ=255)
       agaCAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCgg                                                                                                                                         >  2:2361942/1‑148 (MQ=255)
         aCAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCg                                                                                                                                       <  2:2872588/148‑1 (MQ=255)
          cAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGa                                                                                                                                      <  1:1193356/148‑1 (MQ=255)
              ttCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTgg                                                                                                                                  >  2:3456331/1‑148 (MQ=255)
              ttCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTgg                                                                                                                                  <  2:2795741/148‑1 (MQ=255)
               tCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGc                                                                                                                                 <  2:1254820/148‑1 (MQ=255)
                  cAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCttt                                                                                                                              <  1:2758069/148‑1 (MQ=255)
                    gCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTAt                                                                                                                            >  1:2419043/1‑148 (MQ=255)
                     cACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAATAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTAtt                                                                                                                           >  2:2783459/1‑148 (MQ=255)
                     cACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTAtt                                                                                                                           >  2:3183399/1‑148 (MQ=255)
                      aCGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTAttt                                                                                                                          <  2:839215/148‑1 (MQ=255)
                       cGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTtc                                                                                                                         >  1:2191380/1‑148 (MQ=255)
                         gAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTtctc                                                                                                                       <  1:3277147/148‑1 (MQ=255)
                          aaGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCa                                                                                                                      <  2:1118457/148‑1 (MQ=255)
                            ggCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCAtg                                                                                                                    <  1:1460758/148‑1 (MQ=255)
                               cAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTgg                                                                                                                 <  1:1861144/148‑1 (MQ=255)
                                 aTGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGAt                                                                                                               >  1:2474097/1‑148 (MQ=255)
                                 aTGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGAt                                                                                                               >  2:1792000/1‑148 (MQ=255)
                                       gTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCa                                                                                                         <  2:412793/148‑1 (MQ=255)
                                       gTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCGCa                                                                                                         <  2:2073441/148‑1 (MQ=255)
                                                tCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGc                                                                                                <  1:2352037/148‑1 (MQ=255)
                                                 ctgctgAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCa                                                                                               <  1:940393/148‑1 (MQ=255)
                                                 ctgctgAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATGTCTCATGTGGATACCTCACCGGATTGCa                                                                                               <  2:131316/148‑1 (MQ=255)
                                                       aaaGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCa                                                                                         >  1:3052863/1‑148 (MQ=255)
                                                         agagCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCaaa                                                                                       >  1:523077/1‑148 (MQ=255)
                                                          gagCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCaaaa                                                                                      <  1:3535928/148‑1 (MQ=255)
                                                           agcagcTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCaaaaa                                                                                     >  1:3678272/1‑148 (MQ=255)
                                                           agcagcTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCaaaaa                                                                                     <  1:1023243/148‑1 (MQ=255)
                                                           agcagcTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCGATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCaaaaa                                                                                     >  2:1645625/1‑148 (MQ=255)
                                                               gcTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAAtgtg                                                                                 >  1:2320783/1‑148 (MQ=255)
                                                               gcTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAAtgtg                                                                                 >  2:3073106/1‑148 (MQ=255)
                                                               gcTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAAtgtg                                                                                 >  2:2969706/1‑148 (MQ=255)
                                                                ctctAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGa                                                                                >  2:1973770/1‑148 (MQ=255)
                                                                 tCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGaa                                                                               <  1:2903404/148‑1 (MQ=255)
                                                                 tCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGaa                                                                               <  1:1353754/148‑1 (MQ=255)
                                                                  cGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAAt                                                                              <  2:1136767/148‑1 (MQ=255)
                                                                     agagATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCg                                                                           >  1:1220067/1‑148 (MQ=255)
                                                                     agagATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCg                                                                           >  2:1450301/1‑148 (MQ=255)
                                                                         aTGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCaaa                                                                       >  1:1104357/1‑148 (MQ=255)
                                                                         aTGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCaaa                                                                       >  1:1085960/1‑148 (MQ=255)
                                                                            gggCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAAttg                                                                    <  1:3475389/148‑1 (MQ=255)
                                                                            gggCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAAttg                                                                    >  1:1488084/1‑148 (MQ=255)
                                                                            gggCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAAttg                                                                    >  1:3649206/1‑148 (MQ=255)
                                                                            gggCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAAttg                                                                    >  2:65740/1‑148 (MQ=255)
                                                                            gggCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAAttg                                                                    >  1:1767630/1‑148 (MQ=255)
                                                                            gggCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAAttg                                                                    >  2:3033807/1‑148 (MQ=255)
                                                                             ggCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCCAAAATGTGAATCCGCAAAttgt                                                                   >  1:794460/1‑148 (MQ=255)
                                                                                ttATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGa                                                                >  2:1519417/1‑148 (MQ=255)
                                                                                   tCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAATTGTGAATCCGCAAATTGTTGaaaa                                                             >  2:3117936/1‑148 (MQ=255)
                                                                                   tCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGaaaa                                                             <  2:3801188/148‑1 (MQ=255)
                                                                                   tCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGaaaa                                                             <  1:2077682/148‑1 (MQ=255)
                                                                                      aTGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTa                                                          <  1:157337/148‑1 (MQ=255)
                                                                                       tgtgACCTTCAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTAt                                                         <  1:3729921/148‑1 (MQ=255)
                                                                                       tgtgACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTAt                                                         <  1:1840460/148‑1 (MQ=255)
                                                                                       tgtgACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTAt                                                         >  1:3118759/1‑148 (MQ=255)
                                                                                       tgtgACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTAt                                                         <  2:1920943/148‑1 (MQ=255)
                                                                                          gACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATcgc                                                      >  1:1923144/1‑148 (MQ=255)
                                                                                              ttAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTg                                                  >  1:2144661/1‑148 (MQ=255)
                                                                                              ttAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTg                                                  >  1:2144468/1‑148 (MQ=255)
                                                                                               tAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTgg                                                 >  1:2534166/1‑148 (MQ=255)
                                                                                               tAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTgg                                                 >  2:2910292/1‑148 (MQ=255)
                                                                                                    gagaAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGata                                            <  1:3179680/148‑1 (MQ=255)
                                                                                                    gagaAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGata                                            <  1:3629420/148‑1 (MQ=255)
                                                                                                    gagaAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGata                                            <  2:852375/148‑1 (MQ=255)
                                                                                                         gggCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTgcg                                       >  1:1812040/1‑148 (MQ=255)
                                                                                                         gggCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGGGGCGATATTgcg                                       >  1:2011780/1‑148 (MQ=255)
                                                                                                          ggCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTgcgc                                      <  1:3351561/148‑1 (MQ=255)
                                                                                                           gCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATCTTGCGCt                                     >  2:2611016/1‑148 (MQ=255)
                                                                                                           gCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCt                                     <  1:608709/148‑1 (MQ=255)
                                                                                                           gCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCt                                     >  2:1078772/1‑148 (MQ=255)
                                                                                                              cTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTggg                                  >  2:3623140/1‑148 (MQ=255)
                                                                                                              cTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTggg                                  <  2:1500753/148‑1 (MQ=255)
                                                                                                              cTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTggg                                  >  1:1127263/1‑148 (MQ=255)
                                                                                                               tttGATGGCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGt                                 >  1:3003733/1‑148 (MQ=255)
                                                                                                                      gCGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCg                          >  1:2716484/1‑148 (MQ=255)
                                                                                                                       cGACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGa                         >  2:2004692/1‑148 (MQ=255)
                                                                                                                        gACGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGAt                        <  1:3184984/148‑1 (MQ=255)
                                                                                                                         aCGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGTAAACTATCGCGGTGGCGATATTTCGCTGGGTATCGGCGATg                       >  1:1732738/1‑148 (MQ=255)
                                                                                                                         aCGTTACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATg                       >  2:2627814/1‑148 (MQ=255)
                                                                                                                            ttACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCACACCGGATTTCAGCGGCAAAAATGTGAATACGCCAATTGTTGAAAACTAACGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAg                    >  1:3699618/1‑148 (MQ=255)
                                                                                                                             tACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGt                   >  2:2239061/1‑148 (MQ=255)
                                                                                                                             tACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGt                   <  2:3314174/148‑1 (MQ=255)
                                                                                                                             tACCGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGt                   <  1:1519417/148‑1 (MQ=255)
                                                                                                                                cGGCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGtttt                <  2:2323352/148‑1 (MQ=255)
                                                                                                                                  gCTAACGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTAt              <  2:527654/148‑1 (MQ=255)
                                                                                                                                     aaCGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTAtaaa           >  1:761959/1‑145 (MQ=255)
                                                                                                                                     aaCGTCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTATCAc           >  2:2524372/1‑148 (MQ=255)
                                                                                                                                      aCGTCCCTGGCTATATCCCGGCGAGTGGGTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTATCAcc          >  1:2407638/1‑148 (MQ=255)
                                                                                                                                         tCCCTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATAGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTATCACCGGt       >  1:2212025/1‑148 (MQ=255)
                                                                                                                                           ccTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTATCACCGGTTa     >  2:576896/1‑148 (MQ=255)
                                                                                                                                           ccTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTATCACCGGTTa     <  1:438011/148‑1 (MQ=255)
                                                                                                                                            cTGGCTATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCACAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTATCACCGGTTAt    >  1:2848634/1‑148 (MQ=255)
                                                                                                                                             tGGCGATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTATCACCGGTGATg   >  2:3218661/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AGGGGTGAGACAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCGATATCCCGGCGATTGGCTTTATTTCTCATGTGGATACCTCACCGGATTGCAGCGGCAAAAATGTGAATCCGCAAATTGTTGAAAACTATCGCGGTGGCGATATTGCGCTGGGTATCGGCGATGAAGTTTTATCACCGGTTATGT  >  CP009273/1181359‑1181648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑