Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,277,786 G→A 100% D823N (GAC→AAC)  narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,277,7860GA98.7% 510.3 / ‑2.0 157D823N (GAC→AAC) narGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (101/54);  total (103/54)
Fisher's exact test for biased strand distribution p-value = 5.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 157 total aligned reads displayed.

CCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAA  >  CP009273/1277641‑1277931
                                                                                                                                                 |                                                                                                                                                  
ccGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑c                                                                                                                                                 <  1:732334/148‑1 (MQ=255)
  gTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTg                                                                                                                                               >  1:1337467/1‑148 (MQ=255)
       tCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGaca                                                                                                                                          >  2:102384/1‑146 (MQ=255)
       tCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGaaa                                                                                                                                          >  2:102454/1‑148 (MQ=255)
           gCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTa                                                                                                                                      >  2:1265000/1‑148 (MQ=255)
            cACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTAc                                                                                                                                     >  2:1131510/1‑148 (MQ=255)
              ccTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACaa                                                                                                                                   <  2:1084523/148‑1 (MQ=255)
              ccTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACaa                                                                                                                                   >  2:2466839/1‑148 (MQ=255)
                 gTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGc                                                                                                                                >  1:2362709/1‑148 (MQ=255)
                 gTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGc                                                                                                                                >  1:1821207/1‑148 (MQ=255)
                 gTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGc                                                                                                                                >  2:1983112/1‑148 (MQ=255)
                 gTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGc                                                                                                                                >  2:51319/1‑148 (MQ=255)
                  tctcTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGcc                                                                                                                               <  1:873064/148‑1 (MQ=255)
                   ctctATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCa                                                                                                                              >  2:856111/1‑148 (MQ=255)
                       aTTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATcgc                                                                                                                          <  1:3029118/148‑1 (MQ=255)
                        ttCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATcgcg                                                                                                                         >  2:1308347/1‑148 (MQ=255)
                         tCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGACATCTACAAAGCCATCGCga                                                                                                                        >  2:1563999/1‑148 (MQ=255)
                                tcatTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAAtt                                                                                                                 >  2:3165355/1‑148 (MQ=255)
                                 catTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATtc                                                                                                                >  2:3203242/1‑148 (MQ=255)
                                 catTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATtc                                                                                                                >  2:1052246/1‑148 (MQ=255)
                                  atTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATtct                                                                                                               <  2:2518349/148‑1 (MQ=255)
                                    tttGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTcc                                                                                                             >  1:2124994/1‑148 (MQ=255)
                                      tGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGa                                                                                                           <  1:2788568/148‑1 (MQ=255)
                                        ccGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAg                                                                                                         <  1:2711954/148‑1 (MQ=255)
                                         cGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAgt                                                                                                        <  1:1492559/148‑1 (MQ=255)
                                           actgCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGACCCG‑AAAGCAACCTGGGAAAGATAAAAACCCAACCCCACCAAATAATCCGAAgtgt                                                                                                      >  2:2843144/4‑148 (MQ=17)
                                             ggCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGc                                                                                                    <  1:3621329/148‑1 (MQ=255)
                                                    tGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGcc                                                                                             >  2:2332769/1‑148 (MQ=255)
                                                       tACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATc                                                                                          >  1:2561742/1‑148 (MQ=255)
                                                       tACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATc                                                                                          >  2:1717412/1‑148 (MQ=255)
                                                        aCGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCt                                                                                         >  1:382381/1‑148 (MQ=255)
                                                         cGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTg                                                                                        >  1:1000542/1‑148 (MQ=255)
                                                             aaaGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTa                                                                                    <  1:3144949/148‑1 (MQ=255)
                                                             aaaGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTa                                                                                    >  1:3251627/1‑148 (MQ=255)
                                                             aaaGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTa                                                                                    >  1:3434070/1‑148 (MQ=255)
                                                                gacgacATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAg                                                                                 >  2:2871078/1‑148 (MQ=255)
                                                                   gacATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGTCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGaaa                                                                              <  2:545690/148‑1 (MQ=255)
                                                                   gacATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGaaa                                                                              <  1:2284205/148‑1 (MQ=255)
                                                                    acATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAAc                                                                             <  2:3330878/148‑1 (MQ=255)
                                                                     cATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAAcc                                                                            >  2:408078/1‑148 (MQ=255)
                                                                      aTGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTTCGTCGGCCATCTGGGTAAAGAAACCg                                                                           <  2:3494277/148‑1 (MQ=255)
                                                                      aTGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTTCGTCGGCCATCTGGGTAAAGAAACCg                                                                           <  2:3494296/148‑1 (MQ=255)
                                                                        gAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGAc                                                                         <  2:2179110/148‑1 (MQ=255)
                                                                        gAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGAc                                                                         >  2:2457407/1‑148 (MQ=255)
                                                                        gAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGAc                                                                         >  1:2365740/1‑148 (MQ=255)
                                                                        gAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGAc                                                                         >  1:79336/1‑148 (MQ=255)
                                                                          aTACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACAt                                                                       >  2:1385666/1‑148 (MQ=255)
                                                                          aTACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACAt                                                                       <  2:130216/148‑1 (MQ=255)
                                                                          aTACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CAGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACAt                                                                       >  1:2226578/1‑148 (MQ=255)
                                                                           tACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATc                                                                      >  1:1213469/1‑148 (MQ=255)
                                                                                 ggATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACg                                                                <  2:2324224/148‑1 (MQ=255)
                                                                                  gATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGc                                                               <  2:2439117/148‑1 (MQ=255)
                                                                                   atatGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCTGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCt                                                              >  1:31709/1‑148 (MQ=255)
                                                                                    tatGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTg                                                             <  1:1390818/148‑1 (MQ=255)
                                                                                       gCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCt                                                          <  1:2487413/148‑1 (MQ=255)
                                                                                       gCATCCGTTTATTAACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑ATGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGAGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCt                                                          >  2:3600942/1‑148 (MQ=255)
                                                                                         aTCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTAt                                                        <  1:1901451/148‑1 (MQ=255)
                                                                                          tCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAGGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATc                                                       >  1:566475/1‑148 (MQ=255)
                                                                                          tCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATc                                                       >  2:3645122/1‑148 (MQ=255)
                                                                                           ccGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATcc                                                      >  2:1190754/1‑148 (MQ=255)
                                                                                           ccGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATcc                                                      >  2:2458757/1‑148 (MQ=255)
                                                                                              tttATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGc                                                   >  2:2922574/1‑148 (MQ=255)
                                                                                              tttATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGc                                                   >  1:1355730/1‑148 (MQ=255)
                                                                                               ttATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCa                                                  >  1:487646/1‑148 (MQ=255)
                                                                                               ttATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCa                                                  <  1:1983112/148‑1 (MQ=255)
                                                                                               ttATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCa                                                  <  1:51319/148‑1 (MQ=255)
                                                                                               ttATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCa                                                  >  2:2576645/1‑148 (MQ=255)
                                                                                                 aTTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATg                                                >  1:2899258/1‑148 (MQ=255)
                                                                                                  ttCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGa                                               <  2:2692306/148‑1 (MQ=255)
                                                                                                      cccGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGActct                                           >  1:791286/1‑148 (MQ=255)
                                                                                                       ccGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTg                                          >  2:1679881/1‑148 (MQ=255)
                                                                                                       ccGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTg                                          >  1:2538311/1‑148 (MQ=255)
                                                                                                       ccGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTg                                          >  1:2538776/1‑148 (MQ=255)
                                                                                                         gCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGcc                                        >  1:3551054/1‑148 (MQ=255)
                                                                                                         gCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGcc                                        >  1:3551070/1‑148 (MQ=255)
                                                                                                          cTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCg                                       <  1:1731463/148‑1 (MQ=255)
                                                                                                                 cggcggTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACt                                <  1:3529604/148‑1 (MQ=255)
                                                                                                                 cggcggTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACt                                <  1:2051117/148‑1 (MQ=255)
                                                                                                                   gcggTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTgg                              <  1:1029871/148‑1 (MQ=255)
                                                                                                                   gcggTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTgg                              <  1:1131510/148‑1 (MQ=255)
                                                                                                                    cggTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGgc                             >  2:3451287/1‑148 (MQ=255)
                                                                                                                    cggTCGATCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGgc                             >  2:3451304/1‑148 (MQ=255)
                                                                                                                           tCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTCAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCg                      >  2:1950570/1‑148 (MQ=255)
                                                                                                                           tCCGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCg                      >  2:3616575/1‑148 (MQ=255)
                                                                                                                            ccGGCCTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGc                     >  1:2604777/1‑148 (MQ=255)
                                                                                                                                ccTGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGa                 <  1:269719/148‑1 (MQ=255)
                                                                                                                                  tGGGAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGAtg               <  2:1821207/148‑1 (MQ=255)
                                                                                                                                    ggAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGAtgtg             >  1:2162353/1‑148 (MQ=255)
                                                                                                                                    ggAAGCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGAtgtg             >  1:2162861/1‑148 (MQ=255)
                                                                                                                                        gCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAg         <  1:1296532/148‑1 (MQ=255)
                                                                                                                                        gCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAg         >  2:2325780/1‑148 (MQ=255)
                                                                                                                                        gCGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCAGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAg         >  2:1904553/1‑148 (MQ=255)
                                                                                                                                         cGAAAAGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGa        >  1:1131869/1‑148 (MQ=255)
                                                                                                                                           aaaaGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACt      >  2:479175/1‑148 (MQ=255)
                                                                                                                                           aaaaGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACt      >  2:1534810/1‑148 (MQ=255)
                                                                                                                                             aaGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTgg    >  2:394415/1‑148 (MQ=255)
                                                                                                                                             aaGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTgg    >  2:1944502/1‑148 (MQ=255)
                                                                                                                                             aaGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTgg    >  2:567682/1‑148 (MQ=255)
                                                                                                                                              aGCAA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGa   >  2:1796073/1‑148 (MQ=255)
                                                                                                                                               gCGA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGaa  >  2:2976600/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGA‑CTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAA  >  CP009273/1277641‑1277931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑