Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,358,855 G→T 100% G123C (GGC→TGC)  puuB → gamma‑glutamylputrescine oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,358,8550GT98.8% 542.0 / ‑1.6 171G123C (GGC→TGC) puuBgamma‑glutamylputrescine oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base T (88/81);  total (88/83)
Fisher's exact test for biased strand distribution p-value = 2.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 174 total aligned reads displayed.

CTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATACC  >  CP009273/1358713‑1358998
                                                                                                                                              |                                                                                                                                               
cTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCtgcggc                                                                                                                                            <  1:1124119/148‑1 (MQ=255)
cTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCtgcggc                                                                                                                                            <  2:2057640/148‑1 (MQ=255)
  ccTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCt                                                                                                                                          <  2:745550/148‑1 (MQ=255)
    tACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGt                                                                                                                                        >  1:3090255/1‑148 (MQ=255)
    tACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGt                                                                                                                                        <  2:1860133/148‑1 (MQ=255)
    tACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGt                                                                                                                                        <  1:3708010/148‑1 (MQ=255)
     aCAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGtt                                                                                                                                       >  2:1062021/1‑148 (MQ=255)
       aGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGAAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTtg                                                                                                                                     >  2:1680524/1‑148 (MQ=255)
        gCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTtgt                                                                                                                                    <  1:1700531/148‑1 (MQ=255)
         ccGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATTTTCGAAGGTGGTGAGATAATCCGCGAACTTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTtgtg                                                                                                                                   <  1:2472439/148‑1 (MQ=255)
             gACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGa                                                                                                                               <  1:700425/148‑1 (MQ=255)
              aCATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGat                                                                                                                              <  2:1944080/148‑1 (MQ=255)
              aCATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGat                                                                                                                              >  2:398325/1‑148 (MQ=255)
                aTCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGatga                                                                                                                            <  2:553028/148‑1 (MQ=255)
                aTCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGatga                                                                                                                            <  2:2516165/148‑1 (MQ=255)
                 tCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGatgaa                                                                                                                           <  2:1708686/148‑1 (MQ=255)
                 tCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGatgaa                                                                                                                           <  1:242842/148‑1 (MQ=255)
                   gACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGatgaatg                                                                                                                         <  2:1985365/148‑1 (MQ=255)
                    aCGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGatgaatga                                                                                                                        >  1:1536631/1‑148 (MQ=255)
                     cGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGAt                                                                                                                       >  1:553583/1‑148 (MQ=255)
                     cGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGAt                                                                                                                       >  1:626093/1‑148 (MQ=255)
                      gTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATa                                                                                                                      <  1:2029325/148‑1 (MQ=255)
                        gATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATaaa                                                                                                                    <  1:1946993/148‑1 (MQ=255)
                         aTCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAAc                                                                                                                   <  2:433346/148‑1 (MQ=255)
                          tCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACa                                                                                                                  >  1:838275/1‑148 (MQ=255)
                          tCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACa                                                                                                                  >  2:2890806/1‑148 (MQ=255)
                          tCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACa                                                                                                                  >  2:2148711/1‑148 (MQ=255)
                          tCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACa                                                                                                                  >  1:1967156/1‑148 (MQ=255)
                           cGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAg                                                                                                                 >  2:1885113/1‑148 (MQ=255)
                           cGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAg                                                                                                                 <  1:1939456/148‑1 (MQ=255)
                           cGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAg                                                                                                                 >  1:2355320/1‑148 (MQ=255)
                           cGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAg                                                                                                                 <  1:3494751/148‑1 (MQ=255)
                            gAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGc                                                                                                                <  1:2668367/148‑1 (MQ=255)
                                aaaGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGc                                                                                                            >  1:2264327/1‑148 (MQ=255)
                                  aGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCca                                                                                                          >  1:1038303/1‑148 (MQ=255)
                                  aGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCca                                                                                                          <  2:626093/148‑1 (MQ=255)
                                         gCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGa                                                                                                   >  2:1437276/1‑148 (MQ=255)
                                            tGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCACACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAaga                                                                                                >  2:3562320/1‑148 (MQ=255)
                                             ggACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAagag                                                                                               >  2:671298/1‑148 (MQ=255)
                                             ggACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAagag                                                                                               >  2:2781067/1‑148 (MQ=255)
                                             ggACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAagag                                                                                               >  1:1246947/1‑148 (MQ=255)
                                             ggACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAagag                                                                                               >  2:671769/1‑148 (MQ=255)
                                                  ccGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGaa                                                                                          <  1:3601681/148‑1 (MQ=255)
                                                    gCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAag                                                                                        <  1:661167/148‑1 (MQ=255)
                                                     cccGTTTGGTGGGCAGCATGTTGTTTTAAGGTTGTTAGATCATCCGCGAAAGTTTCAAACGTTATCAGATTGACTGCGACTAGCGACCCTGCGGCCTGTTTGTTGCGATGAATGATAAACAGCTCGCCTCACTTGAATAGCAGAAaga                                                                                       <  2:1958849/148‑1 (MQ=255)
                                                     cccGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAaga                                                                                       >  1:3745541/1‑148 (MQ=255)
                                                     cccGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAaga                                                                                       >  2:1703862/1‑148 (MQ=255)
                                                        gTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGaa                                                                                    <  2:336217/148‑1 (MQ=255)
                                                        gTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGaa                                                                                    <  1:1342339/148‑1 (MQ=255)
                                                         tATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAACGAGAAc                                                                                   >  2:3312531/1‑148 (MQ=255)
                                                          aTGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGCAAGAGAACt                                                                                  >  1:3425875/1‑148 (MQ=255)
                                                             cTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTggg                                                                               >  2:2966037/1‑148 (MQ=255)
                                                              tCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGa                                                                              <  2:1314553/148‑1 (MQ=255)
                                                                 gcagcaTGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACg                                                                           >  2:1407678/1‑148 (MQ=255)
                                                                   agcaTGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCt                                                                         <  2:1118634/148‑1 (MQ=255)
                                                                    gcaTGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTa                                                                        <  1:1703862/148‑1 (MQ=255)
                                                                     caTGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTAc                                                                       >  1:2202710/1‑148 (MQ=255)
                                                                        gatgTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGACCGCTACGGc                                                                    >  1:1169218/1‑148 (MQ=255)
                                                                        gatgTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGc                                                                    >  1:3214801/1‑148 (MQ=255)
                                                                         atgTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCa                                                                   <  1:3682249/148‑1 (MQ=255)
                                                                              cGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTTACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATaaa                                                              <  2:3365317/148‑1 (MQ=255)
                                                                                  ggtggtGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGc                                                          >  2:1237219/1‑148 (MQ=255)
                                                                                    tggtgAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTg                                                        <  1:1012076/148‑1 (MQ=255)
                                                                                       tgAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGaa                                                     <  1:2148711/148‑1 (MQ=255)
                                                                                       tgAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGaa                                                     <  2:347821/148‑1 (MQ=255)
                                                                                       tgAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGaa                                                     >  1:2314580/1‑148 (MQ=255)
                                                                                       tgAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGaa                                                     <  2:347841/148‑1 (MQ=255)
                                                                                        gagaTCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAAt                                                    <  1:1092335/148‑1 (MQ=255)
                                                                                            tcatcCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCt                                                >  2:2651368/1‑148 (MQ=255)
                                                                                               tcCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGa                                             >  2:2789000/1‑148 (MQ=255)
                                                                                                     aaCGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGacgccaa                                       >  2:154651/1‑148 (MQ=255)
                                                                                                      aCGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGacgccaac                                      >  2:3607395/1‑148 (MQ=255)
                                                                                                         tATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGacgccaacgcc                                   >  1:1904673/1‑148 (MQ=255)
                                                                                                            cAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCAtt                                >  2:247597/1‑148 (MQ=255)
                                                                                                            cAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCAtt                                >  2:243490/1‑148 (MQ=255)
                                                                                                            cAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCAtt                                <  2:3090255/148‑1 (MQ=255)
                                                                                                            cAAACGTTATCAGATTGACTGCGACTACCGCCCATGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACTGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCAtt                                >  1:3154706/1‑148 (MQ=255)
                                                                                                              aaCGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCg                              >  1:11784/1‑148 (MQ=255)
                                                                                                              aaCGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCAGTCg                              >  2:2694420/1‑148 (MQ=255)
                                                                                                               aCGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGc                             >  1:2296050/1‑148 (MQ=255)
                                                                                                                     tCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGaa                       <  2:2968834/148‑1 (MQ=255)
                                                                                                                      cAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAg                      <  1:2306527/148‑1 (MQ=255)
                                                                                                                      cAGATTGACTGCGACTACCGCCCCTGCGGCCTGTGTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAg                      <  2:785405/148‑1 (MQ=255)
                                                                                                                          ttGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGc                  >  2:631307/1‑148 (MQ=255)
                                                                                                                           tGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGcc                 <  1:3524969/148‑1 (MQ=255)
                                                                                                                           tGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGcc                 <  2:418292/148‑1 (MQ=255)
                                                                                                                               tGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCg             <  2:3147758/148‑1 (MQ=255)
                                                                                                                               tGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCg             >  1:45398/1‑148 (MQ=255)
                                                                                                                               tGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCg             >  1:45439/1‑148 (MQ=255)
                                                                                                                                gCGACTACCGCCCCTGCGGCCTGTTTGTGTCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGa            >  1:1017623/1‑148 (MQ=255)
                                                                                                                                gCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGa            >  1:705163/1‑148 (MQ=255)
                                                                                                                                gCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGa            >  1:2645823/1‑148 (MQ=255)
                                                                                                                                gCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGa            >  1:2618676/1‑148 (MQ=255)
                                                                                                                                   aCTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCg         >  2:364057/1‑148 (MQ=255)
                                                                                                                                   aCTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCg         <  2:3491294/148‑1 (MQ=255)
                                                                                                                                   aCTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCg         >  2:3345895/1‑148 (MQ=255)
                                                                                                                                   aCTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCg         >  1:1084058/1‑148 (MQ=255)
                                                                                                                                      aCCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCta      >  1:3077718/1‑148 (MQ=255)
                                                                                                                                       ccGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCtat     >  2:2070554/1‑148 (MQ=255)
                                                                                                                                          ccccTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATAcc  >  1:408973/1‑148 (MQ=255)
                                                                                                                                              |                                                                                                                                               
CTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATACC  >  CP009273/1358713‑1358998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑