Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 10,444 G→T 100% F17L (TTC→TTA)  satP ← succinate‑acetate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927310,4440GT99.6% 774.2 / ‑4.2 233F17L (TTC→TTA) satPsuccinate‑acetate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (95/137);  total (95/138)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 233 total aligned reads displayed.

AACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGG  >  CP009273/10299‑10588
                                                                                                                                                 |                                                                                                                                                
aaCCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTaa                                                                                                                                                >  2:1741714/1‑148 (MQ=255)
aaCCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTaa                                                                                                                                                >  2:251300/1‑148 (MQ=255)
  ccGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGc                                                                                                                                              >  2:3515261/1‑148 (MQ=255)
      aaGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCAt                                                                                                                                          >  1:3281211/1‑148 (MQ=255)
      aaGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCAt                                                                                                                                          >  2:2205564/1‑148 (MQ=255)
         tgtTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAg                                                                                                                                       >  1:521598/1‑148 (MQ=255)
            tGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGcc                                                                                                                                    >  1:1330200/1‑148 (MQ=255)
             gCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGccc                                                                                                                                   <  2:1710085/148‑1 (MQ=255)
             gCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGccc                                                                                                                                   >  1:2375804/1‑148 (MQ=255)
              ccTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCa                                                                                                                                  >  2:604465/1‑148 (MQ=255)
                ttttttGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGc                                                                                                                                >  2:2316324/1‑148 (MQ=255)
                ttttttGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGc                                                                                                                                >  2:3642406/1‑148 (MQ=255)
                 tttttGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCg                                                                                                                               >  2:1391684/1‑148 (MQ=255)
                 tttttGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCg                                                                                                                               >  1:3550997/1‑148 (MQ=255)
                 tttttGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCg                                                                                                                               >  1:3534474/1‑148 (MQ=255)
                   tttGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGt                                                                                                                             <  1:1717603/148‑1 (MQ=255)
                    ttGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTg                                                                                                                            <  1:1458498/148‑1 (MQ=255)
                       tACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCg                                                                                                                         <  1:2565100/148‑1 (MQ=255)
                        aCTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCgg                                                                                                                        <  1:450153/148‑1 (MQ=255)
                           ccAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGAtt                                                                                                                     <  2:1644602/148‑1 (MQ=255)
                            cagcagACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTa                                                                                                                    <  2:3234817/148‑1 (MQ=255)
                            cagcagACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTa                                                                                                                    <  1:3533041/148‑1 (MQ=255)
                               cagACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGcc                                                                                                                 >  1:2409152/1‑148 (MQ=255)
                                  aCCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAAc                                                                                                              >  1:604299/1‑148 (MQ=255)
                                  aCCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAAc                                                                                                              >  1:1953567/1‑148 (MQ=255)
                                   ccAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACt                                                                                                             <  2:2314935/148‑1 (MQ=255)
                                   ccAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACt                                                                                                             >  1:2219043/1‑148 (MQ=255)
                                     aGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTa                                                                                                           <  2:1067108/148‑1 (MQ=255)
                                     aGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTa                                                                                                           <  2:2386252/148‑1 (MQ=255)
                                      gCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAg                                                                                                          >  2:1275983/1‑148 (MQ=255)
                                              ttGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGccc                                                                                                  <  1:1794912/148‑1 (MQ=255)
                                                   cgATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAAt                                                                                             <  2:2274121/148‑1 (MQ=255)
                                                     aTGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTc                                                                                           <  1:902572/148‑1 (MQ=255)
                                                      tGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTcc                                                                                          <  2:3706231/148‑1 (MQ=255)
                                                            cgTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCaaaa                                                                                    >  1:3225036/1‑148 (MQ=255)
                                                            cgTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCaaaa                                                                                    <  1:3505481/148‑1 (MQ=255)
                                                              tAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAat                                                                                  <  2:3550997/148‑1 (MQ=255)
                                                               aGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAatc                                                                                 <  2:1330258/148‑1 (MQ=255)
                                                                gAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAatca                                                                                >  1:2085865/1‑148 (MQ=255)
                                                                 aaaaTGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAatcat                                                                               <  1:1365681/148‑1 (MQ=255)
                                                                   aaTGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAatcatca                                                                             <  1:2801484/148‑1 (MQ=255)
                                                                   aaTGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAatcatca                                                                             <  1:602664/148‑1 (MQ=255)
                                                                   aaTGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAatcatca                                                                             <  1:3102063/148‑1 (MQ=255)
                                                                    aTGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCACCTTAGTGTTGCCCATAATTCCTCAAAAatcatcat                                                                            >  2:2739837/1‑148 (MQ=255)
                                                                    aTGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAatcatcat                                                                            <  2:3334347/148‑1 (MQ=255)
                                                                     tGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAatcatcatc                                                                           >  1:283657/1‑148 (MQ=255)
                                                                     tGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAatcatcatc                                                                           >  1:1401221/1‑148 (MQ=255)
                                                                       cccATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCga                                                                         <  2:387013/148‑1 (MQ=255)
                                                                        ccATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCgaa                                                                        <  2:308941/148‑1 (MQ=255)
                                                                         cATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCgaat                                                                       <  1:3070702/148‑1 (MQ=255)
                                                                         cATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCgaat                                                                       <  1:393005/148‑1 (MQ=255)
                                                                            ggCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCgaatgaa                                                                    <  1:3515261/148‑1 (MQ=255)
                                                                             gCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCgaatgaat                                                                   <  1:284651/148‑1 (MQ=255)
                                                                              cAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGaatgaatg                                                                  <  2:840090/148‑1 (MQ=255)
                                                                              cAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGaatgaatg                                                                  <  1:2583867/148‑1 (MQ=255)
                                                                                   ataataCCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGaa                                                             >  1:2051479/1‑148 (MQ=255)
                                                                                   ataataCCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGaa                                                             <  2:1702857/148‑1 (MQ=255)
                                                                                      ataCCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAaata                                                          <  1:3384623/148‑1 (MQ=255)
                                                                                        aCCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAaataat                                                        >  1:2904530/1‑148 (MQ=255)
                                                                                        aCCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAaataat                                                        <  1:251300/148‑1 (MQ=255)
                                                                                        aCCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAaataat                                                        <  1:2179484/148‑1 (MQ=255)
                                                                                          cGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAAttt                                                      <  1:1373918/148‑1 (MQ=255)
                                                                                             ccAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTccc                                                   <  2:889807/148‑1 (MQ=255)
                                                                                                 agCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGaa                                               >  2:821281/1‑148 (MQ=255)
                                                                                                   cGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATa                                             <  2:3004959/148‑1 (MQ=255)
                                                                                                   cGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATa                                             <  2:1137744/148‑1 (MQ=255)
                                                                                                     aaaTAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAAc                                           >  1:1282291/1‑148 (MQ=255)
                                                                                                     aaaTAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAAc                                           >  2:2278380/1‑148 (MQ=255)
                                                                                                      aaTAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACt                                          <  2:19044/148‑1 (MQ=255)
                                                                                                      aaTAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACt                                          <  1:1893557/148‑1 (MQ=255)
                                                                                                      aaTAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACt                                          <  2:338951/148‑1 (MQ=255)
                                                                                                       aTAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTg                                         <  1:1216108/148‑1 (MQ=255)
                                                                                                       aTAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTg                                         <  1:1215901/148‑1 (MQ=255)
                                                                                                         aaCCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTa                                       <  1:3167837/148‑1 (MQ=255)
                                                                                                          aCCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGTCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAg                                      >  1:2104490/1‑148 (MQ=255)
                                                                                                           cccACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAgt                                     >  2:1309948/1‑148 (MQ=255)
                                                                                                           cccACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACGGTAgt                                     >  2:1285990/1‑148 (MQ=255)
                                                                                                             cACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAgtgt                                   >  2:1481027/1‑148 (MQ=255)
                                                                                                             cACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAgtgt                                   <  2:2133186/148‑1 (MQ=255)
                                                                                                             cACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAgtgt                                   >  2:2944390/1‑148 (MQ=255)
                                                                                                              tcGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTATCCAAATTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGtt                                  <  1:1095046/147‑1 (MQ=255)
                                                                                                              aCGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTGGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGtt                                  <  1:1705036/148‑1 (MQ=255)
                                                                                                               cGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGttt                                 >  2:1952291/1‑148 (MQ=255)
                                                                                                                gTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTGAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGtttt                                <  2:521598/148‑1 (MQ=255)
                                                                                                                  tgtgCAGGTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCa                              >  1:2419971/1‑148 (MQ=255)
                                                                                                                         gTTAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGcgcg                       >  2:2593764/1‑148 (MQ=255)
                                                                                                                          ttAAGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCgg                      <  2:1800692/148‑1 (MQ=255)
                                                                                                                             aGCAGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCat                   >  2:3516240/1‑148 (MQ=255)
                                                                                                                                aGAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCataat                <  2:3270865/148‑1 (MQ=255)
                                                                                                                                 gAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCataata               <  2:534263/148‑1 (MQ=255)
                                                                                                                                 gAATGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCataata               <  2:2827267/148‑1 (MQ=255)
                                                                                                                                   aTGGTGGTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATaataat             <  1:1634068/148‑1 (MQ=255)
                                                                                                                                    tggtggTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATc            <  1:1029971/148‑1 (MQ=255)
                                                                                                                                    tggtggTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATc            <  1:2288566/148‑1 (MQ=255)
                                                                                                                                    tggtggTCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATc            <  2:226095/148‑1 (MQ=255)
                                                                                                                                       tggtCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGc         >  2:2019328/1‑148 (MQ=255)
                                                                                                                                       tggtCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGc         <  2:1521896/148‑1 (MQ=255)
                                                                                                                                          tCATGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAg      <  2:2148018/148‑1 (MQ=255)
                                                                                                                                            aTGCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTg    <  2:1050271/148‑1 (MQ=255)
                                                                                                                                              gCCTAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTggg  >  2:3230100/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGG  >  CP009273/10299‑10588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑