Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,477,565 A→T 40.7% K83I (AAA→ATA)  hrpA → putative ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,477,5650AT40.7% 149.3 / 270.2 211K83I (AAA→ATA) hrpAputative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base A (66/59);  new base T (44/42);  total (110/101)
Fisher's exact test for biased strand distribution p-value = 8.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 221 total aligned reads displayed.

GAAGAAGGTTAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCG  >  CP009273/1477419‑1477712
                                                                                                                                                  |                                                                                                                                                   
taagaagTTTAAAAATCCTGATGCACAACAGGACATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCATGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGaaaaaa                                                                                                                                                    <  1:2435315/147‑1 (MQ=255)
gaagaaGGTTAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGaaaaaa                                                                                                                                                    <  1:1733562/148‑1 (MQ=255)
gaagaaGGTTAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGaaaaaa                                                                                                                                                    <  1:356841/148‑1 (MQ=255)
  agaagGTTAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACa                                                                                                                                                  >  1:3775989/1‑148 (MQ=255)
   gaagGTTAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAg                                                                                                                                                 >  1:1210043/1‑148 (MQ=255)
   gaagGTTAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAg                                                                                                                                                 >  1:3673875/1‑148 (MQ=255)
    aagGTTAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAgg                                                                                                                                                >  1:3438879/1‑148 (MQ=255)
    aagGTTAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGAGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAgg                                                                                                                                                <  2:577522/148‑1 (MQ=255)
       gTTAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACa                                                                                                                                             <  2:1822143/148‑1 (MQ=255)
        ttAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACAt                                                                                                                                            >  2:3069061/1‑148 (MQ=255)
        ttAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACAt                                                                                                                                            >  2:62050/1‑148 (MQ=255)
          aaaaaTCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATtc                                                                                                                                          <  1:2943658/148‑1 (MQ=255)
          aaaaaTCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATtc                                                                                                                                          <  1:267777/148‑1 (MQ=255)
          aaaaaTCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATtc                                                                                                                                          <  1:3086209/148‑1 (MQ=255)
           aaaaTCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATtct                                                                                                                                         <  1:1002135/148‑1 (MQ=255)
           aaaaTCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATtct                                                                                                                                         <  1:272954/148‑1 (MQ=255)
            aaaTCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATtctc                                                                                                                                        >  1:1408555/1‑148 (MQ=255)
              aTCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGa                                                                                                                                      >  2:345665/1‑148 (MQ=255)
              aTCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGa                                                                                                                                      >  2:760976/1‑148 (MQ=255)
              aTCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGa                                                                                                                                      >  1:770193/1‑148 (MQ=255)
              aTCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGa                                                                                                                                      >  1:1669729/1‑148 (MQ=255)
               tCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGaa                                                                                                                                     <  1:2029727/148‑1 (MQ=255)
                  tGATGCACGACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCg                                                                                                                                  <  1:138319/148‑1 (MQ=255)
                  tGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCg                                                                                                                                  <  1:840816/148‑1 (MQ=255)
                  tGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCg                                                                                                                                  <  1:3492240/148‑1 (MQ=255)
                    aTGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGAt                                                                                                                                <  2:3094209/148‑1 (MQ=255)
                    aTGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGAt                                                                                                                                >  2:1130662/1‑148 (MQ=255)
                      gCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTc                                                                                                                              >  1:1182257/1‑148 (MQ=255)
                       cacaACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCg                                                                                                                             <  2:225047/148‑1 (MQ=255)
                       cacaACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCg                                                                                                                             <  2:29795/148‑1 (MQ=255)
                        acaacaGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGt                                                                                                                            <  1:3805815/148‑1 (MQ=255)
                         caacaGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTg                                                                                                                           <  1:1077751/148‑1 (MQ=255)
                          aacaGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGa                                                                                                                          >  2:244539/1‑148 (MQ=255)
                             aGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCa                                                                                                                       >  1:555750/1‑148 (MQ=255)
                               gCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCAcc                                                                                                                     >  1:2558445/1‑148 (MQ=255)
                                 cATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAg                                                                                                                   >  2:219666/1‑148 (MQ=255)
                                  aTTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAgg                                                                                                                  >  2:1090936/1‑148 (MQ=255)
                                  aTTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAgg                                                                                                                  <  2:1228797/148‑1 (MQ=255)
                                    tttCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAggtg                                                                                                                >  1:1954532/1‑148 (MQ=255)
                                    tttCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAggtg                                                                                                                <  2:129293/148‑1 (MQ=255)
                                     ttCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGTTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAggtgg                                                                                                               <  1:1334933/148‑1 (MQ=255)
                                        cAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGa                                                                                                            <  2:690269/148‑1 (MQ=255)
                                         aGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTTAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGAt                                                                                                           >  1:2343893/1‑148 (MQ=255)
                                           gagaTGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGAtcg                                                                                                         >  1:1912538/1‑148 (MQ=255)
                                           gagaTGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGAtcg                                                                                                         >  1:3246286/1‑148 (MQ=255)
                                            agaTGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGAtcgt                                                                                                        >  1:2162763/1‑148 (MQ=255)
                                              aTGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGAtcgtcg                                                                                                      >  2:104287/1‑148 (MQ=255)
                                              aTGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGAtcgtcg                                                                                                      >  1:1471514/1‑148 (MQ=255)
                                                    aaaGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGAGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTGACCGGTTAGTCAGAAAAAACACGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCgggg                                                                                                <  2:1585199/148‑1 (MQ=255)
                                                     aaGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGa                                                                                               >  2:240163/1‑148 (MQ=255)
                                                       gagaTTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGaaa                                                                                             >  2:2554010/1‑148 (MQ=255)
                                                        agaTTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGGGGTGATCGTCGCCGGGGacac                                                                                            >  2:3126374/1‑148 (MQ=255)
                                                          aTTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACgg                                                                                          <  1:914430/148‑1 (MQ=255)
                                                           ttGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACggg                                                                                         <  1:2441068/148‑1 (MQ=255)
                                                           ttGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACggg                                                                                         <  2:3790488/148‑1 (MQ=255)
                                                            tGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGt                                                                                        <  2:1738946/148‑1 (MQ=255)
                                                            tGACCAGGCGGCAGGGAAAGTCCTGCCGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGt                                                                                        <  2:3294215/148‑1 (MQ=255)
                                                                  ggcggcAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGACACGGGTTCTGGt                                                                                  >  2:3191184/1‑148 (MQ=255)
                                                                   gcggcAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGGTCTGGTa                                                                                 >  1:1557125/1‑148 (MQ=255)
                                                                     ggcATGGAAAGTCCTGCTGCGTTAAGCTGCACGACCGGTTATTACTTATCCTGACATTTTACCGGTTTGTCTGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGGGATCGTCGCCGGGGAAACGGGTTCTGGTaaa                                                                               <  1:3295323/148‑1 (MQ=255)
                                                                     ggcAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTaaa                                                                               <  1:1880554/148‑1 (MQ=255)
                                                                      gcAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTaaaa                                                                              <  2:1701133/148‑1 (MQ=255)
                                                                      gcAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTaaaa                                                                              <  1:240625/148‑1 (MQ=255)
                                                                      gcAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTaaaa                                                                              <  2:1125249/148‑1 (MQ=255)
                                                                         gggAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGa                                                                           >  1:688147/1‑148 (MQ=255)
                                                                           taaaGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACt                                                                         <  2:3418185/147‑1 (MQ=255)
                                                                            aaaGTCCTGCTGCGTGAAGCGGCACGACCGTAAATTAATTATCCTGAAATTTTCCCGTTTAGTAATAAAAAACAGGACATTCTCGAAGCGATTGGTGATCACCCGGTGGTTATGGTCGCGGGGGAAACGGGTTCTGGTAAAACGACTc                                                                        <  1:3067938/148‑1 (MQ=255)
                                                                            aaaGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTc                                                                        >  1:2844227/1‑148 (MQ=255)
                                                                                           gAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCt                                                         >  2:2248797/1‑148 (MQ=255)
                                                                                             aGCGGCACCACCGTAATTTACTTATCCTTAAAATTGACGGTTTTGTCATAAAAAACAGGACTTTCTCGAAGCGATTCGTGATCACCCGGTGGTGATGGTCGCCGGGTAAAGGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGt                                                       <  1:2328374/148‑1 (MQ=255)
                                                                                              gCGGCACGACCTTAAATTACTTATCCTGACAATTTACCGGTTAGTAAGAAAAAACAGGACATTCTCCAAGCTATTCGTGATCACCAGGTGGTGATCGTCGCCGGGTAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTa                                                      <  2:54569/148‑1 (MQ=255)
                                                                                              gCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTa                                                      >  1:1099603/1‑148 (MQ=255)
                                                                                              gCGGCACGACCGCAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGAACACCCGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTa                                                      <  2:54721/148‑1 (MQ=255)
                                                                                               cGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGCCATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTAt                                                     <  2:1840987/148‑1 (MQ=255)
                                                                                                  cACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGa                                                  >  1:255404/1‑148 (MQ=255)
                                                                                                      aCCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTg                                              >  1:1694969/1‑148 (MQ=255)
                                                                                                          gAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGgc                                          >  2:1088285/1‑148 (MQ=255)
                                                                                                              ttACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCgg                                      >  2:1173945/1‑148 (MQ=255)
                                                                                                              ttACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCgg                                      >  2:1554518/1‑148 (MQ=255)
                                                                                                              ttACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCgg                                      >  1:67733/1‑148 (MQ=255)
                                                                                                               tACTTATCCTGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCggg                                     >  2:406051/1‑148 (MQ=255)
                                                                                                               tACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCggg                                     >  2:704931/1‑148 (MQ=255)
                                                                                                                  ttATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGAtt                                  >  1:2623168/1‑148 (MQ=255)
                                                                                                                        tGACAATTTACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGa                            <  2:2852104/148‑1 (MQ=255)
                                                                                                                         gACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGAc                           >  1:500862/1‑148 (MQ=255)
                                                                                                                         gACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGAc                           >  1:1352832/1‑148 (MQ=255)
                                                                                                                           cAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTg                         >  2:3078668/1‑148 (MQ=255)
                                                                                                                           cAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTg                         >  2:2043686/1‑148 (MQ=255)
                                                                                                                           cAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTg                         >  2:1427718/1‑148 (MQ=255)
                                                                                                                             aTTTACCGGTTAGTCAGAAAATACCGGACATTCTCGAAGCGATTCGTGAACACCTGGTGGTGTTCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGAt                       <  2:1210043/148‑1 (MQ=255)
                                                                                                                               ttACCGGTTAGTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCg                     >  2:2587368/1‑148 (MQ=255)
                                                                                                                                    ggTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCAt                <  2:1538879/148‑1 (MQ=255)
                                                                                                                                     gTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATa               <  2:2558445/148‑1 (MQ=255)
                                                                                                                                      ttAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATAc              >  1:3017466/1‑148 (MQ=255)
                                                                                                                                         gTCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCa           >  1:2308528/1‑148 (MQ=255)
                                                                                                                                          tCAGAAAATACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAg          >  2:2631762/1‑148 (MQ=255)
                                                                                                                                           cAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGc         >  1:780532/1‑148 (MQ=255)
                                                                                                                                           cAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGc         >  1:1432128/1‑148 (MQ=255)
                                                                                                                                              aaaaaaCAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTANAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCcgc      >  1:1937371/1‑148 (MQ=255)
                                                                                                                                                  aaCAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGcgtcg  >  1:610314/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GAAGAAGGTTAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCG  >  CP009273/1477419‑1477712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑