Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,482,005 G→T 100% D172Y (GAT→TAT)  ydcF → DUF218 superfamily protein, SAM‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,482,0050GT99.2% 829.2 / ‑1.9 255D172Y (GAT→TAT) ydcFDUF218 superfamily protein, SAM‑binding
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base T (118/135);  total (118/137)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 255 total aligned reads displayed.

ACAAACTGCGGTGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCGATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGCTGCCGCGTTTAC  >  CP009273/1481861‑1482149
                                                                                                                                                |                                                                                                                                                
acaaACTGCGGTGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATg                                                                                                                                               >  1:3644414/1‑148 (MQ=255)
 caaACTGCGGTGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATNGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGc                                                                                                                                              >  1:1933149/1‑148 (MQ=255)
 caaACTGCGGTGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGc                                                                                                                                              >  2:415737/1‑148 (MQ=255)
 caaACTGCGGTGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGc                                                                                                                                              >  1:1625453/1‑148 (MQ=255)
  aaaCTGCGGTGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGca                                                                                                                                             <  2:871326/148‑1 (MQ=255)
   aaCTGCGGTGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACTATCCGCCGTATGACTGGGGACAATCCCTATGcac                                                                                                                                            >  1:519223/1‑148 (MQ=255)
    aCTGCGGTGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGcacc                                                                                                                                           <  2:3459058/148‑1 (MQ=255)
           tGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTgg                                                                                                                                    <  1:113067/148‑1 (MQ=255)
            gAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGt                                                                                                                                   <  2:932246/148‑1 (MQ=255)
            gAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGt                                                                                                                                   <  1:3365624/148‑1 (MQ=255)
            gAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGt                                                                                                                                   <  2:501827/148‑1 (MQ=255)
              aaaCGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTa                                                                                                                                 >  2:537625/1‑148 (MQ=255)
                acgcacgcTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAg                                                                                                                               >  1:516383/1‑148 (MQ=255)
                 cgcacgcTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGt                                                                                                                              >  1:1910961/1‑148 (MQ=255)
                 cgcacgcTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGt                                                                                                                              >  1:653217/1‑148 (MQ=255)
                     cgcTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATc                                                                                                                          >  2:443651/1‑148 (MQ=255)
                       cTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATccc                                                                                                                        >  2:2367966/1‑148 (MQ=255)
                       cTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATccc                                                                                                                        >  2:3331539/1‑148 (MQ=255)
                        tttAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCg                                                                                                                       >  1:3464195/1‑148 (MQ=255)
                             cATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTc                                                                                                                  <  1:3157207/148‑1 (MQ=255)
                               tCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGt                                                                                                                >  1:1242189/1‑148 (MQ=255)
                               tCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGATATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGACGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGt                                                                                                                <  1:2154778/148‑1 (MQ=255)
                                cgcgCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGtt                                                                                                               <  1:3390539/148‑1 (MQ=255)
                                cgcgCTATTGAATCAGGCAGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATTCAGCGGCGCACGATGGCGACGTTCCGCAGTATGACTGGGGACAATCCCTATGCACAACGCTGGTTAAGTTATCCCGGATTCGtt                                                                                                               <  1:2194059/148‑1 (MQ=255)
                                  cgcTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTcc                                                                                                             >  2:3401295/1‑148 (MQ=255)
                                    cTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTc                                                                                                           <  2:2879731/148‑1 (MQ=255)
                                      aTTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAg                                                                                                         <  2:696487/148‑1 (MQ=255)
                                       ttGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGt                                                                                                        >  2:599580/1‑148 (MQ=255)
                                             cAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGaa                                                                                                  <  1:993021/148‑1 (MQ=255)
                                             cAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGaa                                                                                                  >  2:3807078/1‑148 (MQ=255)
                                             cAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGaa                                                                                                  >  2:3096899/1‑148 (MQ=255)
                                               ggCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAAt                                                                                                <  2:3425054/148‑1 (MQ=255)
                                                gCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATa                                                                                               <  2:2987923/148‑1 (MQ=255)
                                                gCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATa                                                                                               <  1:942598/148‑1 (MQ=255)
                                                gCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATa                                                                                               <  2:3740154/148‑1 (MQ=255)
                                                gCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCGATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATa                                                                                               <  1:2281959/148‑1 (MQ=255)
                                                 ccGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATaa                                                                                              >  2:2774582/1‑148 (MQ=255)
                                                     aGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTACGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCa                                                                                          >  1:906548/1‑148 (MQ=255)
                                                        aCGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGAc                                                                                       <  2:2099570/148‑1 (MQ=255)
                                                        aCGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGAc                                                                                       >  2:1056494/1‑148 (MQ=255)
                                                          gAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAg                                                                                     <  1:2424760/148‑1 (MQ=255)
                                                          gAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAg                                                                                     >  2:11213/1‑148 (MQ=255)
                                                             ttCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAgtgt                                                                                  >  1:1104243/1‑148 (MQ=255)
                                                              tCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTa                                                                                 <  1:2598108/148‑1 (MQ=255)
                                                               cATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTaa                                                                                >  1:667572/1‑148 (MQ=255)
                                                                    ggCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCttt                                                                           <  2:1638035/148‑1 (MQ=255)
                                                                         tCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTaa                                                                      <  2:1178880/148‑1 (MQ=255)
                                                                         tCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTaa                                                                      >  2:3071624/1‑148 (MQ=255)
                                                                          cGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAAt                                                                     <  2:2724802/148‑1 (MQ=255)
                                                                          cGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAAt                                                                     >  1:1286080/1‑148 (MQ=255)
                                                                           gttgttCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATc                                                                    <  1:552856/148‑1 (MQ=255)
                                                                            ttgttCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCa                                                                   <  2:311951/148‑1 (MQ=255)
                                                                            ttgttCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCa                                                                   <  1:3236563/148‑1 (MQ=255)
                                                                             tgttCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAg                                                                  <  2:1281217/148‑1 (MQ=255)
                                                                                  aGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACa                                                             <  2:3651833/148‑1 (MQ=255)
                                                                                  aGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACa                                                             <  1:1458946/148‑1 (MQ=255)
                                                                                    gACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAg                                                           >  1:1017844/1‑148 (MQ=255)
                                                                                    gACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAg                                                           >  2:2474752/1‑148 (MQ=255)
                                                                                     aCCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAgg                                                          <  1:748276/148‑1 (MQ=255)
                                                                                     aCCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAgg                                                          <  1:1542000/148‑1 (MQ=255)
                                                                                     aCCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAgg                                                          <  1:3442505/148‑1 (MQ=255)
                                                                                      ccccACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGa                                                         <  1:1185167/148‑1 (MQ=255)
                                                                                        ccaccaTGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGAtt                                                       <  1:956878/148‑1 (MQ=255)
                                                                                        ccaccaTGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGAtt                                                       <  1:2358102/148‑1 (MQ=255)
                                                                                          accaTGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTAt                                                     <  1:1791070/148‑1 (MQ=255)
                                                                                            caTGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATgg                                                   >  2:776221/1‑148 (MQ=255)
                                                                                              tGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGcc                                                 >  2:3766019/1‑148 (MQ=255)
                                                                                              tGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGcc                                                 >  1:2556749/1‑148 (MQ=255)
                                                                                              tGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGcc                                                 <  1:3060799/148‑1 (MQ=255)
                                                                                                 aGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGt                                              <  1:2017935/148‑1 (MQ=255)
                                                                                                     gcgcACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAg                                          >  2:254367/1‑148 (MQ=255)
                                                                                                     gcgcACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAg                                          <  1:46569/148‑1 (MQ=255)
                                                                                                     gcgcACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAg                                          >  2:666218/1‑148 (MQ=255)
                                                                                                      cgcACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGc                                         >  1:3055859/1‑148 (MQ=255)
                                                                                                          cGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTa                                     >  2:3072965/1‑148 (MQ=255)
                                                                                                            aTGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTAtc                                   >  1:2935113/1‑148 (MQ=255)
                                                                                                               gCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTAtctct                                <  1:1048847/148‑1 (MQ=255)
                                                                                                               gCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTAtctct                                <  1:2200085/148‑1 (MQ=255)
                                                                                                                cGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTAtctctc                               <  1:2835648/148‑1 (MQ=255)
                                                                                                                cGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTAtctctc                               >  1:1438107/1‑148 (MQ=255)
                                                                                                                cGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTAtctctc                               <  2:3627599/148‑1 (MQ=255)
                                                                                                                cGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTAtctctc                               <  1:388077/148‑1 (MQ=255)
                                                                                                                 gACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCa                              <  1:3128760/148‑1 (MQ=255)
                                                                                                                   cGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCact                            >  2:3187604/1‑148 (MQ=255)
                                                                                                                   cGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCact                            <  2:2762369/148‑1 (MQ=255)
                                                                                                                     ttCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATTGCCAGTTGAGCGTTATCTCTCactac                          >  1:1255674/1‑148 (MQ=255)
                                                                                                                     ttCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCactac                          >  1:3376911/1‑148 (MQ=255)
                                                                                                                         gccgTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCaa                      <  1:1120042/148‑2 (MQ=255)
                                                                                                                          ccgTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACt                     <  2:3604447/148‑1 (MQ=255)
                                                                                                                          ccgTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACt                     >  2:836280/1‑148 (MQ=255)
                                                                                                                          ccgTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACt                     <  2:2893519/148‑1 (MQ=255)
                                                                                                                              aTGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCg                 >  1:2858607/1‑148 (MQ=255)
                                                                                                                               tGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGa                >  2:1919690/1‑148 (MQ=255)
                                                                                                                                 aCTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTAGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGTCAAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGc              >  1:3250938/1‑148 (MQ=255)
                                                                                                                                       gACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGCTGCcgc        >  1:2685270/1‑148 (MQ=255)
                                                                                                                                         cAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGCTGCCGCGt      >  1:1035549/1‑148 (MQ=255)
                                                                                                                                          aaTCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGCTGCCGCGtt     >  2:178598/1‑148 (MQ=255)
                                                                                                                                           aTCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGCTGCCGCGttt    <  2:825661/148‑1 (MQ=255)
                                                                                                                                            tCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGCTGCCGCGTTTa   >  1:2739217/1‑148 (MQ=255)
                                                                                                                                             cccTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGCTGCCGCGTTTAc  >  2:1407406/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
ACAAACTGCGGTGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCGATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGCTGCCGCGTTTAC  >  CP009273/1481861‑1482149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑